Effects of processing parameters on immersion vacuum cooling time and physico-chemical properties of pork hams

The effects of agitation (1002 rpm), different pressure reduction rates (60 and 100 mbar/min), as well as employing cold water with different initial temperatures (IWT: 7 and 20 °C) on immersion vacuum cooling (IVC) of cooked pork hams were experimentally investigated. Final pork ham core temperature, cooling time, cooling loss, texture properties, colour and chemical composition were evaluated. The application for the first time of agitation during IVC substantially reduced the cooling time (47.39%) to 4.6 °C, compared to IVC without agitation. For the different pressure drop rates, there was a trend that shorter IVC cooling times were achieved with lower cooling rate, although results were not statistically significant (P > 0.05). For both IWTs tested, the same trend was observed: shorter cooling time and lower cooling loss were obtained under lower linear pressure drop rate of 60 mbar/min (not statistically significant, P > 0.05). Compared to the reference cooling method (air blast cooling), IVC achieved higher cooling rates and better meat quality.     

Feasibility assessment of vacuum cooling of cooked pork ham with water compared to that without water and with air blast cooling

To optimise vacuum cooling for application in the meat industry, an improved cooling method, i.e. vacuum cooling with water (or immersion vacuum cooling), was designed to cool cooked pork ham (2.2 ± 0.2 kg). It was found that the cooling time of vacuum cooling with water was significantly shorter than that of traditional air blast cooling (P < 0.05). For the cooling loss, vacuum cooling with water was significantly lower (6.99%) than that of vacuum cooling without water (13.71%) (P < 0.05). Significant differences in physical and chemical attributes were also observed for ham processed by vacuum cooling with and without water (P < 0.05). Therefore, for a certain size of pork ham, vacuum cooling with water could be an effective method to meet safety guidelines and obtain compatible quality attributes with air blast cooling.     

Vacuum cooling in bulk of beef pieces of different sizes and shape – Evaluation and comparison to conventional cooling methods

The use of vacuum cooling (VC) to chill cooked beef dices, strips and mince in bulk (circa 1 kg samples) to core temperature below 4 °C was investigated. Cooling parameters and some product quality properties were evaluated and compared to those obtained after conventional cooling methods (air blast, cold room and plate cooling). Data on cooling time and profile, mass losses, nutritional content and physical properties (colour and instrumental shear force) were compared. For all studied shapes VC yielded the shortest cooling time. However, benefits of VC mechanisms on cooling time affected studied shapes differently. Diced beef VC time was markedly affected by beef inherent properties, and VC seemed to yield better results when applied to mince than to strips and dices. For all beef shapes studied VC led to higher weight losses (about 10%) than the other cooling methods. Dices and strips showed significantly (P < 0.05) higher protein and lower moisture contents after VC than after the other methods, while for minced beef a significant difference (P < 0.05) was observed only for protein content. Other compositional and quality properties results (salt and fat contents, colour and instrumental tenderness) for the three forms were similar among cooling methods.     

真空冷却-浸入式真空冷却对白煮鸡腿品质影响

以白煮鸡腿为研究对象,使用真空冷却-浸入式真空冷却、真空冷却、浸入式真空冷却、传统的冷却方法风冷、水冷方法比较产品品质和冷却效果。比较结果表明白煮鸡腿的中心温度从72℃降至10℃用真空冷却-浸入式真空冷却方法降温速率最快,并且弹性显著（p<0.05）大于其他冷却方式,a*、剪切力、硬度、胶着性和咀嚼性与水冷无显著性（p>0.05）的差别,各冷却方式的白煮鸡腿pH在一周的贮藏期内无显著性差别（p>0.05）。真空冷却-浸入式真空冷却方法的冷却速率快,且最终冷却产品的品质也较好。      

An innovative method of immersion vacuum cooling for cooked meat products: immersion vacuum cooling with ultrasonic assistance

In this paper, immersion vacuum cooling with ultrasonic assistance (IVCUA) is compared with immersion vacuum cooling alone (IVC) and vacuum cooling (VC) for cooling time, mass loss, colour, texture profile and water mobility and compartmentalization for cooked meat products. The results reveal that IVCUA clearly enhances the boiling intensity of impregnation liquid compared to IVC. The total cooling time using IVC (126.56 min) for samples from 72 to 4 °C was significantly longer (P < 0.05) than that of IVCUA (96.89 min) and VC (80.48 min). The cooling time of samples from 10 to 4 °C by IVC (46.26 min) was higher (P < 0.05) than that by IVCUA (28.55 min). There were no significant differences (P > 0.05) in mass loss, colour and texture profile among all samples. However, IVCUA had a higher transverse relation time of bulk water (T₂₄) and MRI proton densities (P < 0.05), as well as a more uniform water distribution compared to IVC.     

Application of immersion vacuum cooling to water-cooked beef joints - Quality and safety assessment

Immersion vacuum cooling (IVC) was compared against air blast cooling (AB) and vacuum cooling (VC), to establish its applicability to cool water-cooked beef joints. Process parameters (cooling time and cooling loss) and quality parameters (proximate; physical; sensory and microbial analysis) were compared. Results showed IVC samples have lower cooling losses and improved quality properties compared to VC, while generally comparable to AB samples. IVC cooling times were slower then VC, but faster than AB. Sensory results showed no preference between samples (P > 0.05), even though differences in tenderness and juiciness were detected. Cooling methods had no significant effect on thermoduric spore survival or heat activation in the case of Bacillus atrophaeus and Geobacillus stearothermophilus spores. Results confirmed IVC is capable of combining rapid process times and lower mass losses producing microbiologically safe beef joints of overall high quality in terms of sensory, physical and composition characteristics.     

Physico-chemical, textural and structural characteristics of sous-vide cooked pork cheeks as affected by vacuum, cooking temperature, and cooking time

This paper describes the influence of different factors on sous-vide cooked pork. Pork cheeks were cooked at different combinations of temperature (60 °C or 80 °C), time (5 or 12 h) and vacuum (vacuum or air packaged). Weight losses were lower and moisture content higher in samples cooked for a shorter time (P = 0.054) and at a lower temperature (P < 0.001). Samples cooked at 60 °C showed more lightness (L*) and redness (a*) (P < 0.001). Lipid oxidation showed an interaction between cooking time and temperature (P = 0.007), with higher TBARs values for samples cooked for 12 h at 60 °C and lower for those cooked for 12 h at 80 °C. Samples cooked at 80 °C for 12 h showed lower (P < 0.05) values for most textural parameters than all the other types of samples. Vacuum packaging showed no influence on any of the studied variables. For the treatments evaluated, cooking temperature × time combination seems to be more important than vacuum packaging in the textural and colour parameters of pork cheeks.     

真空冷却技术对低温乳化肠品质影响的研究

为了提高熟肉制品的食用品质和延长货架期,探索适当的冷却技术对熟肉制品贮藏具有重要的意义。本实验分别采用流水冷却、风冷却、真空冷却、流水和真空混合冷却方式使天然肠衣包装的乳化香肠的中心温度从80℃降至室温20℃,探究不同的降温方式对低温乳化香肠的品质和货架期影响。结果显示:混合冷却方式耗时明显低于其他单独使用的冷却方式;与真空冷却相比混合冷却方式可以明显降低产品的失水率(p<0.05)、获得更优的感官评价;风冷却使储藏期间的脂肪氧化水平明显提高(p<0.05),真空冷却和混合冷却均可延长货架期,可使乳化香肠的保质期在26d左右,比其他两种方式延长5d。本实验认为利用流水冷却和真空冷却相结合的混合冷却方式更适于天然肠衣包装的乳化型香肠的冷却。      

Evaluation of Bubbling Vacuum Cooling for the Small-Size Cooked Pork

Vacuum cooling has notable advantages including fast cooling rate, cleanness, and high energy efficiency. However, the weight loss of food after being vacuum cooled was unsatisfactory, especially for meat products. Immersion vacuum cooling can significantly reduce the weight loss of food compared with traditional vacuum cooling procedures, but the cooling rate is unacceptable. To overcome this problem, here, a novel vacuum cooler, bubbling vacuum cooler, was designed and evaluated for the small-size cubic cooked pork with a side length of 1.5 cm from about 60 to 4 °C. Experimental results indicated that bubbling vacuum cooling can reduce the weight loss (about ??2.3%) of food compared to both vacuum cooling (about 12.4%) and immersion vacuum cooling (about 0.5%) (P?<?0.05). Further, bubbling vacuum cooling can cool the sample with a slightly more rapid cooling rate (0.10 °C/s) contrasted with immersion vacuum cooling (0.07 °C/s) (P?>?0.05). For the chromatism value of sample, no significant difference was found between immersion vacuum cooling and bubbling vacuum cooling (P?>?0.05). The textural property of sample cooled by bubbling vacuum cooling was close to (for hardness, elasticity, chewiness, and shear force, P?>?0.05) and better (for cohesiveness, P?<?0.05) than that of immersion vacuum cooling. Thus, our experiment demonstrated that cooked pork cooled by bubbling vacuum cooling has a lower weight loss rate and a more rapid cooling rate than that of immersion cooling.     

Immersion vacuum cooling of cooked beef - Safety and process considerations regarding beef joint size

Cooked beef samples (1, 2, and 3kg; 4.7, 5.6, and 6.2cm average radius, respectively) were cooled from ～72 to 4°C core temperature using either air blast (AB), immersion vacuum (IVC) or vacuum (VC) cooling. IVC cooled larger samples within 4h and took less than 2.5h between 72 and 10°C. IVC cooling times were on average shorter than AB and longer than VC for all sizes. Differences increased with size. IVC and AB cooling losses were comparable (P>0.05) while lower on average (P<0.05) than VC losses for same size samples. Additionally, samples between 1.0 and 4.3kg (4.2-8.7cm average radius) were cooled by either IVC or VC. Cooling times were between 2.8 and 5.5h for IVC and between 1.1 and 3.2h for VC. There was a significant effect (P<0.01) of sample size on IVC cooling times. Cooling profiles of larger samples were tested using USDA cooling growth model for Clostridium perfringens in beef broth. According to the model, none of the analyzed profiles would support significant growth of the bacteria.     

Predictive model for growth of Clostridium perfringens during cooling of cooked uncured meat and poultry

Comparison of Clostridium perfringens spore germination and outgrowth in cooked uncured products during cooling for different meat species is presented. Cooked, uncured product was inoculated with C. perfringens spores and vacuum packaged. For the isothermal experiments, all samples were incubated in a water bath stabilized at selected temperatures between 10 and 51 °C and sampled periodically. For dynamic experiments, the samples were cooled from 54.4 to 27 °C and subsequently from 27 to 4 °C for different time periods, designated as x and y hours, respectively. The growth models used were based on a model developed by Baranyi and Roberts (1994. A dynamic approach to predicting bacterial growth in food. Int. J. Food Micro. 23, 277-294), which incorporates a constant, referred to as the physiological state constant, q₀. The value of this constant captures the cells’ history before the cooling begins. To estimate specific growth rates, data from isothermal experiments were used, from which a secondary model was developed, based on a form of Ratkowsky’s 4-parameter equation. The estimated growth kinetics associated with pork and chicken were similar, but growth appeared to be slightly greater in beef; for beef, the maximum specific growth rates estimated from the Ratkowsky curve was about 2.7 log₁₀ cfu/h, while for the other two species, chicken and pork, the estimate was about 2.2 log₁₀ cfu/h. Physiological state constants were estimated by minimizing the mean square error of predictions of the log₁₀ of the relative increase versus the corresponding observed quantities for the dynamic experiments: for beef the estimate was 0.007, while those for pork and chicken the estimates were about 0.014 and 0.011, respectively. For a hypothetical 1.5 h cooling from 54 °C to 27° and 5 h to 4 °C, corresponding to USDA-FSIS cooling compliance guidelines, the predicted growth (log₁₀ of the relative increase) for each species was: 1.29 for beef; 1.07 for chicken and 0.95 log₁₀ for pork. However, it was noticed that for pork in particular, the model using the derived q₀ had a tendency to over-predict relative growth when the observed amount of relative growth was small, and under-predict the relative growth when the observed amount of relative growth was large. To provide more fail-safe estimate, rather than using the derived value of q₀, a value of 0.04 is recommended for pork.     

Evaluation of innovative immersion vacuum cooling with different pressure reduction rates and agitation for cooked sausages stuffed in natural or artificial casing

Effects of different pressure reduction rates and liquid agitation (523 rpm) on sausage successful rate, cooling time, cooling loss, texture properties, chemical and physical parameters were analysed by ANCOVA. Tested linear pressure drop rate from 320 mbar to 50 mbar was 20 (L 20), 30 (L 30), or 40 (L 40) mbar/min for artificial casing sausages (ACS) and 60 (L 60), 80 (L 80), or 100 (L 100) mbar/min for natural casing sausages (NCS). From 50 mbar until 6.4 mbar, 5 mbar/min was used for both casings. NCS were more suitable than ACS for IVC. The recommended pressure reduction rate was 30 mbar/min for ACS and 60 mbar/min for NCS. ACS under 30 mbar/min with agitation (LA 30) presented significantly higher texture property values than that without agitation (L 30) (P < 0.05). Cooling time (to 4 °C) of NCS under 60 mbar/min and agitation (LA 60) was significantly shorter than that without agitation (L 60) (P < 0.05). This study could assist meat processors or manufactures when choosing a suitable pressure drop rate for different types of sausages.     

Comparisons of housing, bedding, and cooling options for dairy calves

Housing, bedding, and summer cooling were management options evaluated. Holstein calves (42 ± 2 kg of body weight) initially 2 to 5 d of age were managed in southwest Ohio in poly hutches or wire mesh pens in a curtain-sided nursery with no supplemental heat. Calves were fed milk replacer (27% crude protein, 17% fat fed at 0.657 kg of dry matter per calf daily), starter (20% crude protein dry matter, textured, fed free-choice), and water (free-choice). Measurements were for 56 d. In trial 1, 28 calves per treatment were bedded with straw and housed in either hutches or nursery pens. This trial was conducted from September to March; the average temperature was 8°C and ranged from −17 to 31°C. In trial 2a, 16 calves per treatment were managed in nursery pens bedded with straw, in nursery pens bedded with sand, or in hutches bedded with sand. This trial was conducted from May to September; the average temperature was 21°C and ranged from 7 to 33°C. In trial 2b, 26 calves per treatment were housed in nursery pens and bedded with straw. This trial was conducted from May to September; the average temperature was 22°C and ranged from 8 to 34°C. One treatment was cooled with fans between 0800 and 1700 h and the other was not. Data were analyzed as repeated measures in a completely randomized block design by trial, with calf as the experimental unit. In trial 3, air in the nursery and calf hutches used above was sampled 35 d apart for calves aged 5 and 40 d. Air in individual hutches on 2 commercial farms was sampled for 5- and 40-d-old calves for 2 hutch types. Air in the multi-calf hutches was sampled for calves of 75 and 110 d of age. Bacterial concentrations of air samples were analyzed (log₁₀) as odds ratios by Proc Logistic in SAS software (SAS Institute Inc., Cary, NC); differences were declared at P < 0.05. In trial 1, weight gain of calves in nursery pens was 6% greater and feed efficiency was 4% greater than that of calves in hutches. In trial 2a, weight gain and starter intake of calves in the nursery with straw bedding were greater and scouring was less than that in calves bedded with sand in the nursery or hutches. The relative humidity was greater in the hutches than in the nursery pens. In trial 2b, weight gain, feed efficiency, and hip width change were greater and breaths per minute were less for calves cooled with fans compared with calves that were not cooled. In trial 3, airborne bacteria concentrations were greater in the hutches than in the nursery pens. Straw bedding (vs. sand), nursery pens (vs. hutches), and summer daytime cooling with fans improved calf weight gain.     

Effects of dry,vacuum, and special bag aging; USDA quality grade; and end-point temperature on yields and eating quality of beef Longissimus lumborum steaks

This study evaluated the effects of three aging methods: (dry (D), wet (W), and special bag (SB)); two quality grades [USDA Choice((≥ Small⁵⁰ marbling) and Select); and two cooked end-point temperatures (62.8 °C and 71.1 °C) on physico-chemical traits of instrumental tenderness, color, and sensory properties of Longissimus lumborum beef muscle. Dry-aged loins had higher (P < 0.0001) weight loss than W or SB aged loins. However, D and SB aged loins had similar (P > 0.05) combined losses. W aged loins had higher (P < 0.01) L* values than D or SB aged loins. Warner–Bratzler shear force of steaks was not affected (P > 0.05) by aging method or quality grade but increased (P < 0.0001) as end-point temperature increased. Sensory panel evaluation also showed no effect (P > 0.05) of aging method or quality grade on myofibrillar tenderness, juiciness, connective tissue amount, overall tenderness or off flavor intensity. Steaks cooked to 62.8 °C were juicier (P < 0.05) than those cooked to 71.1 °C. Neither D nor SB aging had advantages over W aging.     

Oxidation in HiOx-packaged pork Longissimus muscle predisposes myofibrillar and sarcoplasmic proteins to N-nitrosamine formation in nitrite-curing solution

The effect of meat protein in situ oxidation on the formation of N-nitrosodiethylamine (NDEA) was investigated. Fresh minced pork was untreated (Con) or treated with 700 mg/kg α-tocopherol (Toc) or 300 mg/kg tea polyphenols (PPE), packaged in a HiOx atmosphere (78.8% O₂, 18.8% CO₂, 2.4% N₂), then stored at 2 ± 1 °C for up to 10 days. Crude myofibrillar (MP) or sarcoplasmic (SP) protein (20 mg/mL) extracted from stored meat was reacted with 43 μM sodium nitrite at 80 °C for 1 h. Lipid oxidation was totally inhibited in PPE pork but increased in Con and Toc samples after 10 days. There was significant protein oxidation (losses of sulfhydryls, formation of protein carbonyls) in both MP and SP in all samples during storage. However, the Con group suffered more extensive protein oxidation than Toc and PPE and produced more NDEA (P < 0.05), indicating that protein oxidation promoted nitrosation.     

EFFECTS OF COMBINED WATER COOKING–VACUUM COOLING WITH WATER ON PROCESSING TIME, MASS LOSS AND QUALITY OF LARGE PORK HAM

The catering system of pork ham is normally a cook–chill system, which is based on the full cooking of pork ham followed by chilling. In order to obtain large pork ham with high quality and safety, a novel method was developed in this work, i.e., combined water cooking–vacuum cooling with water (immersion vacuum cooling) (WC+VW). The effects of WC+VW on processing time, mass loss and quality of large pork ham were evaluated by comparing them with those of four traditional pork ham processing methods, i.e., dry air cooking combined with air blast cooling (DAC+AB), dry air cooking combined with cold room cooling (DAC+CR), wet air cooking combined with air blast cooling (WAC+AB) and wet air cooking combined with cold room cooling (WAC+CR). WC+VW achieved the shortest processing time, resulting from the shortest time of vacuum cooling with water (308.0 min). Furthermore, present results showed that WC+VW could produce mass loss and quality of large pork ham comparable to those of the four traditional processing methods.     

Modified atmosphere packaging and vacuum packaging for long period chilled storage of dry-cured Iberian ham

Dry-cured Iberian ham slices were stored under vacuum and under four different modified atmospheres (60/40 = 60%N₂ + 40%CO₂; 70/30 = 70%N₂ + 30%CO₂; 80/20 = 80%N₂ + 20%CO₂; argon = 70%argon + 30%CO₂) at 4 ± 1 °C during 120 days. Gas composition, moisture content, pH, colour, pigment content, and lipid stability were measured, as well as sensory and microbial analysis were carried out throughout storage. A loss of intensity of red colour (a^*-values) was observed during storage in ham slices (P < 0.05). Consistently, MbFe(II)NO content also decreased throughout storage (P > 0.05). Slices of ham packed in 40%CO₂ (60/40) and 30%CO₂ (70/30) showed lower a^*-values than the rest of the batches after 60 days (P < 0.05), though differences were not evident after 120 days (P > 0.05). TBARs values showed an upward trend during the storage of packaged slices (P < 0.05). Vacuum-packed slices showed the lowest TBARs values and those packed with 40%CO_2, the highest. Sensory attributes did not vary significantly (P > 0.05) throughout storage under refrigeration and packed either in vacuum or in modified atmospheres. No safety problems were detected in relation to the microbial quality in any case.     

A simple,reliable and reproductive method to obtain experimental pale,soft and exudative (PSE) pork

The inferior quality and economic risk of pale, soft and exudative (PSE) pork warrant continuing research. However, such research efforts are often hindered by the challenge to obtain reliable PSE muscle samples with similar quality characteristics. The objective of this study was to establish a reliable and convenient method to produce PSE-like pork. A PSE condition was induced by incubation of 30-min postmortem Longissimus muscle at 35 °C for 7 h followed by chilling to 4 °C. Compared to normal red, firm and non-exudative (RFN) pork (kept at 4 °C), PSE muscle had consistently lower pH_2h (5.46 vs. 5.74) and pH_4h (5.35 vs. 5.52), higher L* (lightness) value (56.5 vs. 51.0), and reduced protein solubility and thermal stability (enthalpy and temperature) than RFN muscle (P < 0.05). The highly reproducible results indicate that incubation of muscle immediately postmortem at 35 °C offers a simple and consistent method to produce PSE pork.     

Temperature- and pH-dependent effect of lactate on in vitro redox stability of red meat myoglobins

Our objective was to evaluate the influence of lactate on in vitro redox stability and thermostability of beef, horse, pork, and sheep myoglobins. Lactate (200 mM) had no effect (P > 0.05) on redox stability at physiological (pH 7.4, 37 °C) and meat (pH 5.6, 4 °C) conditions. However, lactate increased (P < 0.05) metmyoglobin formation at a condition simulating stressed live skeletal muscle (pH 6.5, 37 °C). The redox stability of myoglobins at stressed live skeletal muscle and meat conditions was species–specific (P < 0.05). Myoglobin thermostability at 71 °C was lower (P < 0.05) in the presence of lactate compared with controls and was influenced (P < 0.05) by species. The results of the present study indicate that the effects of lactate on myoglobin are temperature and pH dependent. The observed lack of influence of lactate on myoglobin redox stability at meat condition suggests that the color stability of lactate-enhanced fresh meat is not due to direct interactions between the ingredient and the heme protein.     

The physicochemical and microbiological characteristics of pork jerky in comparison to beef jerky

This study was carried out to compare the physicochemical and microbiological characteristics of beef and pork jerky, prepared from whole muscle of beef semimembranosus (BSM), pork semimembranosus (PSM), pork longissimus dorsi (PLD), and pork psoas major (PPM). The BSM and PSM jerky had higher moisture content, and PPM jerky had lower water activity than other jerky samples during 30 days of storage at 25 °C (P < 0.05). Pork jerky samples had higher lightness value than beef jerky, while PSM jerky had higher pH value than other jerky samples (P < 0.05). The shear force and TBARS values of PPM jerky were higher than those of other jerky samples (P < 0.05). Saturated fatty acid (SFA, %) was significantly higher in the BSM jerky than others, while unsaturated fatty acid (UFA, %) was significantly higher in the PSM and PLD than BSM and PPM jerky samples (P < 0.05). The PPM jerky showed a significant increase in UFA (%) during storage, and a significantly decrease in microbial count after storage of 30 days (P < 0.05).