澳洲茄胺盐酸盐对小鼠肝癌H22抑制作用研究

目的 研究观察澳洲茄胺盐酸盐对移植性实体型肿瘤小鼠肝癌H22的瘤重抑制率.方法 本项研究采用肿瘤细胞悬液接种法制备小鼠移植性实体型肿瘤H22动物模型.腹腔注射给药,连续10 d.给药结束后,解剖小鼠,取瘤称质量,进行统计学处理及分析,观察药物作用,连续3次重复实验.结果 经过研究发现:澳洲茄胺盐酸盐对移植性实体型小鼠肝癌H22抑制实验3次重复实验证实,在药物倍数剂量设置下,11mg/kg剂量组与盐水对照组比较,有显著性差异;44mg/kg剂量组瘤质量抑制率在55.1%～73.7%之间,平均抑瘤率为62.3%,P＜0.01.结论 通过连续3次重复实验,澳洲茄胺盐酸盐对小鼠移植性实体型肿瘤肝癌H22有明显的抑制作用.     

核桃楸树皮乙酸乙酯提取物对原位移植荷胃癌小鼠的抑瘤作用

目的:研究核桃楸树皮乙酸乙酯提取物(HYT)对原位移植荷胃癌小鼠的抑瘤作用及其对免疫功能的影响,阐明HYT抗胃癌作用机理,为研发低毒高效的抗胃癌中草药提供理论依据.方法:建立HYT灌胃的原位移植荷胃癌小鼠研究模型,分为HYF高剂量组(60 mg·kg-1)、HYF低剂量组(40 mg·kg-1)、假手术组、阳性对照组(5-FU)及阴性对照组,观察每组小鼠抑瘤率及胸腺、脾脏指数,采用ELISA法及流式细胞术等检测荷胃癌小鼠IL-4 、IL-12、TNF-α水平及T淋巴细胞亚群情况.结果:HYT高剂量组抑瘤率与阳性对照组比较差异无统计学意义(P>0.05);与阴性对照组比较,HYT高剂量组小鼠胸腺指数及脾脏指数明显增加(P<0.05),IL-12水平升高(P<0.05),IL-4水平降低(P<0.05),TNF-α水平升高(P<0.05),T淋巴细胞亚群CD4+/CD8+比值升高(P<0.05).结论:HYT可以增强机体免疫功能,具有明确的抑瘤作用,可以作为抗胃癌低毒高效的中草药.     

海藻色素糖蛋白对小鼠H22肝癌细胞增殖与凋亡的影响

目的:研究麒麟菜海藻色素糖蛋白(seaweed pigment glycoprotein,SPG)对小鼠H22肝癌细胞增殖与凋亡的影响.方法:将不同浓度SPG与小鼠H22肝癌细胞共同培养,用MTT法测定癌细胞增殖活性.建立H22移植瘤小鼠肝癌模型,随机分为SPG高、中、低剂量组,对照组及环磷酰胺组.实验组小鼠每天分别给予不同剂量(100、50、10 mg/kg)的SPG灌胃,连续10 d,对照组同法给予等量生理盐水,环磷酰胺组小鼠腹腔注射20 mg/kg环磷酰胺,隔日一次.各组小鼠均于末次给受试物后24 h处死,取肿瘤组织用免疫组化法检测各组Bcl-2和Bax蛋白表达.结果:SPG高剂量组瘤细胞增殖活性(0.545±0.002)明显高于对照组(0.404±0.008)(P<0.05);SPG高剂量组Bcl-2和Bax蛋白阳性表达率分别为16.78%和38.1%,对照组分别为65.16%和4.68%,两组间的差异具有统计学意义(P<0.05).结论:SPG具有抑制小鼠H22肝癌细胞增殖、促进其凋亡的作用.     

人参三七提取物抗疲劳作用的研究

[目的]研究人参三七提取物缓解体力疲劳的作用,为进一步开发利用人参三七提供试验依据.[方法]将小鼠分为4批,每批48只,随机分为4组,分别为对照组和50、100、300 mg/kg 3个给药组,各组小鼠经口灌胃给药30 d后,分别测定其负重游泳时间及血清尿素氮、血乳酸、肝糖原含量.[结果]100和300 mg/kg 剂量组人参三七提取物小鼠负重游泳时间显著长于对照组(P<0.01),血清中尿素氮含量显著低于对照组(P<0.01;P<0.05),小鼠肝糖原水平明显高于对照组(P<0.01;P<0.05);小鼠游泳后血乳酸含量显著低于对照组(P<0.01;P<0.05).[结论]人参三七提取物具有缓解体力疲劳的作用.     

中剂量螺旋藻多糖和银杏提取物的联合抗衰老作用研究

[目的]研究中剂量螺旋藻多糖和银杏提取物的联合抗衰老作用.[方法]每天对试验小鼠颈下注射D-半乳糖125 mg/kg,连续42d,建立衰老模型.给药组小鼠每天灌服100 mg/kg螺旋藻多糖、银杏叶及复合药液,观察中剂量复合螺旋藻多糖和银杏叶有效成分对D-半乳糖致衰老小鼠的作用,并测定免疫器官重量、血清SOD活性和心肌、脑丙二醛(MDA)含量等相关衰老指标.[结果]螺旋藻多糖和银杏提取物均能有效对抗D-半乳糖所致小鼠衰老,二者的复合药效要强于单一药效.[结论]该研究为螺旋藻多糖和银杏提取物的联合抗衰老作用提供了试验依据.     

苯并(a)芘和滴滴涕联合暴露对小鼠肝脏细胞凋亡的影响

目的:探讨不同剂量苯并(a)芘[benzo(a)pyrene,B(a)P]、滴滴涕(chlomphenothane,DDT)单独及联合暴露对小鼠肝脏细胞的毒性效应.方法:成年雌性昆明种小鼠66只,随机分为11组:分别为0.5、5、50 mg/(kg·d)B(a)P染毒组,0.025、0.25、2.5mg/(kg·d)DDT染毒组,0.5 mg/(kg·d)B(a)P+0.025 mg/(kg·d)DDT联合染毒组,5 mg/(kg·d)B(a)P+0.25 mg/(kg·d)DDT联合染毒组,50 mg/(kg·d)B(a)P+2.5 mg/(kg·d)DDT联合染毒组,空白对照组(正常饲养)和溶剂对照组(植物油处理).染毒组用含B(a)P、DDT的食用油进行腹腔注射,每天1次,连续21 d,于末次给药24 h后处死小鼠.取肝脏制作冰冻切片,利用原位缺口末端标记(terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling,TUNEL)法检测肝细胞凋亡情况.结果:5和50 mg/(kg·d)B(a)P染毒组小鼠肝脏细胞凋亡率显著高于对照组(P<0.05),且50 mg/(kg·d)剂量组显著高于0.5和5 mg/(ks·d)剂量组(P<0.05);各DDT染毒组与对照组比较,差异无统计学意义(P>0.05);各浓度联合染毒组小鼠肝脏细胞凋亡率均高于对照组(P<0.01),各联合染毒组细胞凋亡率间的差异无统计学意义(P>0.05),联合染毒组细胞凋亡率和相应的B(a)P染毒组比较,差异无统计学意义(P>0.05).结论:B(a)P的单独暴露以及与DDT的联合暴露均可导致小鼠肝脏细胞凋亡的发生,并可能引发其他毒性效应.     

胡桃楸水提物的遗传毒性及抗诱变作用研究

目的:探讨胡桃楸水提物(juglans mandshurica maxim extract,JMME)的抗诱变作用,及评价胡桃楸是否具有遗传毒性.方法:取80只昆明小鼠,随机分成不同JMME剂量的诱变组(6.55、13.09、26.18 g/kg,分别为1/8、1/4、1/2 LD50),不同剂量的JMME与环磷酰胺(cyclophosphamide,CP)(0.04 g/kg)联合应用的抗诱变组,阴性对照组(Ns)和阳性对照组(CP,0.04 g/kg),共8组,每组10只.采用小鼠骨髓细胞微核试验(MNT)检测JMME是否具有遗传毒性及抗诱变作用.结果:阳性对照组微核发生率(28.82‰)明显高于阴性对照组(2.65‰),其差异具有统计学意义(P<0.01),而JMME各剂量组与阴性对照组间的差异无统计学意义(P>0.05).抗诱变各组的微核发生率与阳性对照组比较,除JMME低剂量组(6.55 g/kg)无统计学意义外(P>0.05),JMME中剂量组(13.09 g/kg)和高剂量组(26.18 g/kg)均显著低于阳性对照组(P<0.05),其中高剂量组明显低于中剂量组(P<0.05).结论:在本实验条件下,JMME无遗传毒性,JMME对CP诱发的微核率,具有一定抵抗作用,且呈量-效关系.     

大红袍茶降血糖作用研究

[目的]探讨大红袍茶的降血糖作用.[方法]取50只小鼠以200 mg/kg剂量、间日2次腹腔注射四氧嘧啶制造高血糖模型,4 d后得到24只血糖高于9.00 mmol/L的高血糖模型小鼠.将高血糖小鼠按相似血糖水平分为对照组和试验组,血糖值分别为(15.11±6.47)和(14.77±5.40)mmol/L,组间无显著差异(P＞0.05).对照组饲喂基础饲料和凉开水,试验组在基础饲料中添加3.0%的大红袍茶,饮0.5%的大红袍茶汤.饲养7 d后再次测定血糖值,研究其变化.[结果]对照组小鼠血糖值为(12.23±5.11)mmol/L,无显著变化(P＞0.05);试验组小鼠血糖值下降到(7.93±1.88)mmol/L,降低了46.3%,变化极显著(P＜0.001).[结论]大红袍茶对高血糖小鼠有极显著的降糖作用.     

排毒清火凉茶粉的急性毒性和致突变性实验研究

目的:研究排毒清火凉茶粉的急性经口毒性和致突变性,对其安全性作出评价.方法:采用最大耐受剂量法观察动物急性毒性反应和死亡情况,测定受试物最大耐受量(MTO).采用Ames试验、小鼠精子畸变试验和骨髓细胞微核试验测定排毒清火凉茶粉的致突变性.结果:小鼠对排毒清火凉茶粉的最大耐受量为36.0 g/kg,大鼠的最大耐受量为18.0 g/kg.Ames试验结果显示,凉茶粉5个剂量(每皿分别为100、500、1 000、2 500、5 000 μg)下的TA97、TA98、TA100、TA102菌株回变菌落数与阴性对照比较,差异均无统计学意义(P均>0.05).小鼠精子畸变试验显示,凉茶粉各剂量组(3、6、12 g/kg)小鼠精子畸形率在1.75%～1.92%,与阴性对照组(1.72%)比较,差别无统计学意义(P>0.05).小鼠骨髓微核试验显示,凉茶粉各剂量组(3、6、12g/kg)小鼠骨髓嗜多染红细胞微核率为0.4‰～1‰,与阴性对照组(0.6‰～0.8‰)比较,差别无统计学意义(P>0.05).结论:排毒清火凉茶粉属实际无毒物质.在本实验条件下,未显示其有致突变作用.     

五味子多糖对甲状腺癌细胞株SW579凋亡及survivin表达的影响

目的:探讨五味子多糖对人甲状腺癌SW579细胞株的生长抑制作用及其机制.方法:48只成年雄性Wistar大鼠分为阴性对照组和药物组,阴性对照组予以生理盐水,药物治疗组予以不同剂量的五味子多糖,分别提取其血清.SW579细胞分为空白组、阴性对照组和药物组.空白组为15%小牛血清的RPMI-1640培养液,阴性对照组细胞加入阴性组大鼠的纯化血清,药物组分别加入不同浓度(100、200 和 400 mg·kg-1)的含药血清,AO/EB荧光染色和流式细胞仪检测细胞凋亡情况,MTT法检测细胞增殖情况,Western blotting检测细胞survivin的表达.结果:流式细胞术结果显示,低、中和高剂量药物组细胞凋亡率为8.63%、35.63%和38.32%,明显高于空白对照组和阴性对照组细胞凋亡率(4.23%和4.10%)(P<0.01),并随剂量增加而增加.阴性对照组细胞生长抑制率为(0.12±0.06)%,低、中和高剂量药物组细胞生长抑制率为(27.51±1.62)%、(34.69±0.79)% 和(45.83±1.33)%,药物组与阴性对照组比较差异有统计学意义(P<0.01).Western blotting 检测低、中、高剂量药物组细胞survivin的表达明显低于空白对照组和阴性对照组(P<0.01).结论:五味子多糖能有效抑制人甲状腺癌细胞株SW579中survivin基因表达,诱导SW579细胞凋亡,抑制肿瘤细胞增殖.     

转炉炉壳热应力分析

根据实际转炉建立实体模型。以有限元为手段,考虑了炉衬和炉壳材料的物性参数随温度变化的特点和炉衬与炉壳之间膨胀间隙,计算了转炉炉壳在温度载荷和炉衬膨胀压力同时作用下的热应力。所得结果可为转炉炉壳设计提供依据。     

焦炭热性能指标意义浅析

通过对焦炭在高炉内的历程及其变化规律的剖析,阐述焦炭热性能指标的意义,形象地把焦炭在高炉内的损耗分为三部分(M1,M2,M3)。指出M2对焦比及焦炭价格有一定的指导作用。     

Results of treatment of cancer of the cardia

Cancer of the cardia is traditionally discussed with cardiac cancer of the lower portion of the esophagus and upper gastric cancer invading the esophagus, and the specific characteristics of cancer of the cardia have never been clearly defined. We reviewed the outcome of 172 patients with adenocarcinoma of the cardia who had undergone radical surgery between 1949 and 1994 in the Division of Gastrointestinal Surgery of the Cancer Institute Hospital. The centers of the tumors were located within 2 cm above and below the boundary between the esophagus and the stomach, and their longitudinal diameter was less than 8 cm. We divided the patients into an early period (1949-1979; n = 79) and a late period (1980-1994; n = 93), and focused on the historical transition. There were no differences in patient gender or histology between the two periods. However, the late period was associated with fewer cases of esophageal invasion and shorter longitudinal diameter, as the age of the population advanced. The number of advanced cancers such as the localized and infiltrative type had decreased, and early cancer and early clinical stage had become more common, but despite the fact that the number of early cancers had increased, extended dissection, such as thoracic and paraaortic lymph node dissection, was performed more frequently. By clinical stage, the long-term outcome markedly improved in Stage I patient in the late period, and tended to improve in Stage II and III patients. This appears to have been attributable to the prevention of micrometastasis by extended dissection, although the number of early cancers is another major potential cause. There were no differences in the outcome of Stage IV patients between the two periods, and further advances in multimodality therapy must be awaited. The range of resection is basically proximal gastrectomy, and if there are adequate indications, the prognosis is favorable. In view of the status of lymphatic flow and lymph node metastasis, and long-term results, lateroaortic lymph node dissection is important. Since the number of early cancer patients has been increasing, if intraabdominal recurrence is prevented, intrathoracic lymph node dissection will contribute greatly to the outcome of such patients.     

The effect of amount of possession of the trait of hostility on accuracy of perception of hostility in others.

Some theories of personality hold that an individual's perception of others is influenced by his own personality characteristics; more specifically, that a trait of low social value will be projected more into others, and that insight into the possession of these characteristics reduces this distortion. These 2 hypotheses were tested by having a group of college students rate each other (including themselves) on degree of friendliness or hostility. Agreement with group rating about self yielded the insight score. In general, the hypotheses were supported; the "friendly" Ss were most accurate in their perception of others, and insight was positively associated with accurate perception. From Psyc Abstracts 36:04:4HL16M. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effect of antibiotics of chemotaxis of human leukocytes

The effect of 20 different antibiotics on chemotaxis by human neutrophils was studied. Human leukocytes incubated with chloramphenicol, rifampin, sodium fusidate, and tetracyclines in vitro showed markedly depressed migration. The mechanisms by which these antibiotics affect leukotaxis are discussed.     

Quality of life of relatives of mental patients

Usage of quality of life of psychiatric patients' relatives as the parameter of estimation of the quality of psychiatric care is considered. By means of standard questionnaire elaborated by the authors as well as by means of both individual and group conversations the quality of life was analyzed in 40 relatives which lived together with psychiatric patients. Different spheres of their life and difficulties (financial, social-psychologic, production) were examined in families with such patients. The defects of psychiatric care that had direct influence on the quality of life of the psychiatric patients' relatives are outlined.