Effect of tuberculosis on milk production in dairy cows

A survey of Isospora suis performed in 177 faecal samples from 30 swine farms detected thin wall type I. suis oocysts in seven samples. This type of oocyst measuring 23.9 by 20.7 microns had a retracted thin wall similar to that of the genus Sarcocystis. This type of oocysts, isolated from four different faecal samples, was inoculated in four-five-days-old piglets free of contamination in order to verify the life cycle and pathogenicity of the species. The pigs were kept in individual metal cages and fed with cow milk. Daily faecal collections and examinations were performed until the 21st day after infection. MacMaster and Sheather's methods were used for oocyst counting and identification. Infected piglets produced yellowish-pasty diarrhoea with slight dehydration. The prepatent and patent periods were respectively from 6 to 9 and 3 to 10 days after infection. Oocyst elimination was interrupted on the 10th and 11th days after infection with biphasic cycles. Thin and thick wall oocysts were detected in the same faecal samples. Thin walls were not observed in unsporulated oocytes. The observations suggest that this type of oocysts could appear in specific strains which occur in the later stages of their development. These oocysts seem to be responsible for clinical and pathogenic signs of neonatal isosporosis in pigs.     

Influence of level of deoxynivalenol in the diet of dairy cows on feed intake, milk production, and its composition

Eighteen primiparous Holstein cows were used in a 10-wk lactation study, preceded by a 2-wk covariate period, to determine the effect of concentration of deoxynivalenol in the diet on cow performance and transfer of deoxynivalenol and its metabolite, deepoxydeoxynivalenol, to milk. Diets were formulated to contain deoxynivalenol at 0, 6, and 12 mg/kg of concentrate DM, and daily intake of deoxynivalenol was .59, 42, and 104 mg, respectively. Increasing deoxynivalenol in the diet did not affect intake of concentrate or forage. Total milk output was not affected; however, milk fat responded quadratically; cows given deoxynivalenol at 6 mg/kg of concentrate DM had the lowest milk fat content and fat output. Overall energetic efficiency was not influenced because reduced energy output in milk was compensated by increased BW gains. No transfer of deoxynivalenol or deepoxydeoxynivalenol to milk was observed; concentrations were below detectable limits (1 microgram/ml) using HPLC-mass spectroscopy. We concluded that diets containing deoxynivalenol up to 6 mg/kg of dietary DM did not reduce feed intake of cows in this study and that deoxynivalenol or deepoxydeoxynivalenol was not transferred to milk. Further studies are required to confirm the apparent lack of effect of deoxynivalenol on milk production.     

Effect of Neospora caninum infection on milk production in first-lactation dairy cows

OBJECTIVE: To determine whether cows seropositive to Neospora caninum produced less milk during their first lactation than seronegative cows. DESIGN: Repeated-measures, prospective study. ANIMALS: 372 Holstein cows in their first lactation. PROCEDURE: Cows were tested repeatedly before and during their first lactation for antibodies to N caninum. One-way and repeated-measures ANOVA were used to determine whether mean daily milk weights and milk weights from Dairy Herd Improvement Association testing were less for seropositive cows than for seronegative cows. RESULTS: Weekly mean daily milk weights for the 118 seropositive cows were significantly less than those for the 254 seronegative cows, and milk production for seropositive cows (mean, 55.2 lb/cow/d) was 2.5 lb/cow/d less than that for seronegative cows (mean, 57.7 lb/cow/d). Analysis of results from Dairy Herd Improvement Association testing revealed that production of seropositive cows was less for milk (3.1 lb/cow/d), fat-corrected milk (3.6 lb/cow/d), and fat (0.14 lb/cow/d) than production of seronegative cows. CLINICAL IMPLICATIONS: The economic impact of N caninum infection in dairy cows can include reduced revenues from decreased milk production, which may warrant culling of young, seropositive replacement stock.     

Effect of infused volatile fatty acids and caseinate on milk composition and coagulation in dairy cows

Milk protein secretion is changed by increasing the proportion of energy, mainly as propionic acid, or the availability of AA. Whether associative effects exist between energy nature and protein amounts is unknown. Therefore, ruminal isoenergetic infusions of low or high propionate mixtures were combined factorially with duodenal infusion of sodium caseinate or control. Four ruminally and duodenally fistulated Holstein cows were used. The diet was limited and consisted of 70% forage and 30% concentrate. Caseinate infusion increased milk yield and protein and casein contents and decreased milk fat content; curd yields and coagulation properties of milk were improved. The infusion of propionic acid caused a large increase in rumen propionate. Milk yield tended to decrease, and milk fat decreased, but protein, casein, and curd yields were unchanged; milk-coagulating properties were improved. No interaction existed between energy and protein amounts. Alteration of VFA had little effect on milk composition, but increasing the protein supply to the duodenum increased milk protein.     

The effect of enhanced milk yield of dairy cows by frequent milking or suckling on intake and digestibility of the diet

The prevalence of osteoporosis and the incidence of fractures are substantially lower in black than in white subjects, a finding generally attributed to racial differences in adult bone mass. Whether these racial differences are present in childhood is the subject of considerable interest, as the amount of bone gained during growth is a major determinant of future susceptibility to fractures. We measured the density and size of the vertebrae and femurs of 80 black and 80 white healthy children, 8-18 yr of age, matched for age, gender, height, weight, and stage of sexual development, using computed tomography. Race had a significant and differential effect on the bones in the axial and appendicular skeletons. In the axial skeleton, black children had greater cancellous bone density, but similar cross-sectional area of the vertebral bodies. In contrast, in the appendicular skeleton, black children had greater femoral cross-sectional area, but similar cortical bone area and cortical bone density. Compared to white children, vertebral bone density and femoral cross-sectional area at sexual maturity were, on the average, 10.75% and 5.7% higher, respectively, in black children. Such significant variations may contribute to the racial differences in the prevalence of osteoporosis between black and white adults.     

Modeling the impact of mastitis on milk production by dairy cows

Individual milk production curves of 542 cows with 722 cases of mastitis were compared with control curves drawn from healthy lactating cows. First, differences were classified into patterns of milk loss, and their distribution was analyzed among the patterns with regard to breed, season, lactation number, stage of lactation, and milk production. Then, individual milk losses were estimated and analyzed according to the same factors. In early lactation, almost 7% of mastitis cases necessitated culling or drying off. For 36% of the cases of mastitis occurring in early lactation, milk production was affected for an extended period, and the milk loss induced was 911 kg on average over the entire lactation. Fifty-two percent of the mastitis cases occurring after lactation peak was not accompanied by marked modifications of the lactation curves. For 38% of cases of mastitis occurring from mid to late lactation, milk production was affected for an extended period. When milk production was the most affected (26% of total cases), the average loss was 850 kg over the entire lactation. For cases in early or mid to late lactation, the production at mastitis onset was a determining factor of the amount and pattern of milk production loss induced. Results were applied in a simulation over two simulated groups of cows.     

Breed group effects on milk production of Brazilian crossbred dairy cows

Lactation records (n = 2362) of 1402 crossbred cows in 22 cooperating dairy herds in southeastern Brazil were evaluated. Cows were mixtures of Zebu (Gir, Guzera, and unknown) and European breeding (mostly Holstein). Lactation milk yields were expressed as total, 3050-d, or deviated 305-d yields, either adjusted or unadjusted for days in milk (DIM). Mean DIM was 280. Arithmetic means unadjusted for DIM were 1942, 1666, and 5 kg per record. Milk yields of daughters from sires of 6/8 and 7/8 European breeds were higher than yields of daughters from sires of 5/8 European breeds when data were either adjusted or unadjusted for DIM. The differences associated with breed group of sire were only slightly reduced when records were adjusted for DIM. There was no evidence of a decline in milk yield as the fraction of European breeding of the sire increased from 6/8 to 7/8. For a given breed group of sire, whether the grandsire was purebred or crossbred had no detectable effect. These results should be useful in determining strategies for crossbreeding of dairy cows in tropical areas, particularly when crossbred sires are used.     

Alteration of milk fat by variation in the source and amount of starch in a total mixed diet fed to dairy cows

The effect on milk yield and composition of the supplementation of the diets of dairy cows with wheat or potato peelings was studied at three different starch intakes (< 5, 6, and > 7.5 kg/d) for dry matter intakes around 20 kg/d. Starch supply was varied using different dietary concentrations of corn silage. Trials were conducted using Latin square designs, and all cows were fed a total mixed diet composed of corn silage, grass silage, or both; soybean meal; a mixed meal with formaldehyde treatment; minerals; and one of the following energy concentrates: potato peelings or wheat. Dry matter and energy intakes varied significantly only at the low starch concentration; higher intakes were observed when the potato peelings were fed. Body weight, milk yield, true protein content, lactose content, and protein yield were not affected by treatment. Milk fat content was higher (+3.3 g/kg) when potato peelings were fed at the high starch concentration but was unaffected at the low and medium starch concentration. Slow degradation of starch from the potato peelings in the rumen could enhance a higher delivery of precursors of milk fat synthesis in the udder. The effect on fat yield differed among starch concentrations. Milk fat content appeared to decrease for cows fed diets containing quickly degradable starch at a starch intake > 7 kg/d.     

Effect of soybean hulls, soy lecithin, and soapstock mixtures on ruminal fermentation and milk composition in dairy cows

To clarify the regulatory mechanism of pro-gelatinase A (proGelA) activation at a cellular level, expression of gelatinase A (GelA), three MT-MMPs, and TIMP-2 was examined with 11 human cancer cell lines cultured in the presence and absence of stimulants. MT1-MMP mRNA was expressed in 8 cell lines, while MT2-MMP and MT3-MMP mRNAs were expressed in fewer cell lines. The cells with high proGelA activation strongly expressed MT1-MMP mRNA but not MT2-MMP and MT3-MMP mRNAs, suggesting that MT1-MMP was responsible for the proGelA activation in the cancer cells. Treatments with concanavalin A (Con A) and a phorbor ester (TPA) enhanced the MT1-MMP expression, but only Con A stimulated the proGelA activation in many cell lines. In HT1080 fibrosarcoma cells, however, TPA also stimulated the activation. The level of TIMP-2 secreted into culture medium inversely correlated with proGelA activation. For example, 2 squamous cell carcinoma lines (HSC-3 and HSC-4) and 3 HT1080 clones, which efficiently activated proGelA, secreted little TIMP-2 into medium, whereas other cell lines and other HT1080 clones, which hardly activated proGelA, secreted TIMP-2 at high levels. When HSC-3 cells were incubated with TIMP-2 protein or transfected with TIMP-2 cDNA, the proGelA activation was strongly inhibited. These results indicated that extracellular TIMP-2 was an important negative regulator of proGelA activation. However, the level of extracellular TIMP-2 was not consistent with that of TIMP-2 mRNA in some cell lines. Other experimental results suggested that TIMP-2 might be rapidly metabolized after binding to MT1-MMP, and Con A treatment might stabilize the complex of TIMP-2 and MT1-MMP on cell membranes.     

Effect of monensin on milk production and efficiency of dairy cows fed two diets differing in forage to concentrate ratios

Four primiparous Holstein cows were gradually introduced, according to a Latin square design, to four diets obtained from the factorial combination of two forage to concentrate ratios (70:30 and 50:50) and two concentrations of monensin sodium (0 and 300 mg/d per cow). Addition of monensin tended to depress feed intake and milk fat content without affecting milk production and without interactions with forage to concentrate ratios. Ruminal propionate percentage was increased more by the addition of monensin to the low forage diet than by the addition of monensin to the high forage diet. Serum urea and concentrations of nonesterified fatty acids tended to decrease when monensin was added to the high forage diet but did not change when monensin was added to the low forage diet. The results suggested that monensin had moderate positive effects on efficiency of milk production and might have an antiketogenic effect with high forage diets.     

Effect of extreme walking conditions for dairy cows on milk yield, chemical composition, and somatic cell count

Thirty-two cows (16 Montbeliardes and 16 Tarentaises) in midlactation were used in an experiment utilizing a 2 x 2 factorial arrangement of treatments. Throughout the trial, cows received first-cutting cocksfoot hay for ad libitum intake supplemented with a fixed amount of concentrate that was individually adapted to the milk yield of each cow. During a 23-d experimental period, one group of cows walked 9.6 km/d; the other group of cows remained in the barn. Cows that walked daily ate less hay (-1.3 and -2.1 kg/d of dry matter for Tarentaise and Montbeliarde cows, respectively) and yielded less milk (-1.7 and -2.5 kg/d for Tarentaise and Montbeliarde cows, respectively) than did cows that did not walk daily. A residual effect of walking on milk yield was observed during the 10 d following the experimental period. For both breeds, fat content and, to a lesser extent, protein content were higher (+6.4 and +1.0 g/kg, respectively) for cows that walked. Somatic cell count was also higher for cows that walked (+115,000 cells/ml). This difference was more marked in cows that were initially infected by a minor or major pathogen (+185,000 cells/ml) than in uninfected cows (+47,000 cells/ml) and on the 1st d of walking when walking was linked to increases in pH, bovine serum albumin, and immunoglobulin G1 contents of milk (+0.08 unit, +0.16 g/L, and +0.19 g/L, respectively). Throughout the experimental period, walking induced a rise in body temperature (+1 degree C) and in plasma nonesterified fatty acids (+0.63 mM/L). On the 1st d of walking, plasma glucose, lactic acid, and cortisol contents were significantly higher for cows that walked (+0.25 g/L, +0.64 g/L, and +28.8 ng/ml, respectively).     

Feed intake and milk production in dairy cows fed whole fat soybeans]

Although the effect of hyperoxia on antioxidant enzymes is well known, the effect of subtoxic levels of hyperoxia on gamma-glutamyltransferase (gamma-GT), involved in the degradation and uptake of extracellular GSH for intracellular GSH synthesis, is unknown. The aim of the study was to investigate (1) the effects of in vitro hyperoxia on gamma-GT activity of type II cells and (2) the effects of the lazaroid U-74389G and N-acetylcysteine (NAC) on the hyperoxia-induced changes in gamma-GT and antioxidant enzyme activities. At 48 h after isolation, rat type II cells were exposed for 2 days to air, 60% O2 or 85% O2 with or without 30 microM U-74389G or 100 microM NAC. After the exposure, the cells were harvested and assayed for superoxide dismutase (SOD), glutathione peroxidase (GPx), gamma-GT activity, and GSH levels. In another series of experiments 85% O2-exposed cells, with or without U-74389G, were used for Northern blotting of gamma-GT mRNA. Exposure to 60% O2 decreased gamma-GT and GSH by -47 and -34%, respectively, while SOD and GPx activities remained unchanged. After 85% O2-exposure gamma-GT decreased by -55%, SOD and GPx increased by +55 and +87%, respectively, while GSH decreased by -35%. NAC treatment decreased gamma-GT activity by -42% in the air-exposed cells. After 60% O2, U-74389G led to significantly higher gamma-GT (+117%) and GSH (+26%) while NAC only led to higher GSH (+28%) compared to the oxygen-exposed cells not treated with NAC or U-74389G. After 85% O2 U-74389G increased gamma-GT, SOD, and GSH by +72, +58, and +68%, respectively, while NAC only increased SOD (+49%) and GSH (+26%) compared to the oxygen-exposed cells not treated with NAC or U-74389G. The 85% O2 exposure, with or without U-74389G, had no effect on gamma-GT mRNA levels. The results show that hyperoxia decreases rat type II cell gamma-GT activity in vitro. This effect was not related to an altered regulation at mRNA level and it was not associated with the hyperoxia-induced decrease in intracellular GSH, since restoration of the GSH levels by NAC did not restore gamma-GT activity. The lazaroid U-74389G with vitamin E-like properties effectively prevented the decrease in gamma-GT and GSH, so that direct inactivation of the membrane-bound gamma-GT by hyperoxia is the most likely mechanism.     

Effects of monensin on the reproduction, health, and milk production of dairy cows

A randomized clinical trial including 1109 cows from 12 Australian dairy herds was used to evaluate the effects of monensin on the health (n = > 686 cows), production (n = 915 cows), and reproduction (n = > 908 cows) of dairy cows. Cows were allocated to a treatment group receiving a slow-release intraruminal bolus containing 32 g of sodium monensin that was administered 40 d before and 50 d following the anticipated calving date or to a control group. Treatment did not significantly alter any reproductive outcome; 54.5% of cows treated with monensin and 58.2% of control cows were pregnant at first service, and days to conception were lower for cows treated with monensin. The hazard rate (0.95) was not significant for these cows. The percentage of cows pregnant was 83.8 for control cows, and days to first estrus (hazard rate = 1.04) and first service (hazard rate = 1.04) were not significantly higher for treated cows. Treatment with monensin did not significantly alter the risk of any disease. The incidence of retained fetal membranes, pyometra, lameness, abortion, and infectious diseases was not significantly lower for cows in the treatment group, and the incidence of mastitis was not significantly higher for cows in the treatment group. Monensin significantly increased milk production by 0.75 L/d per cow and tended to increase milk fat and protein yields but had no significant effect on milk fat or milk protein percentages. Changes in the production of milk and milk constituents were consistent throughout lactation.     

Effect of steam-flaked or steam-rolled corn with or without Aspergillus oryzae in the diet on performance of dairy cows fed during hot weather

The objective of this study was to determine the effects of steam-rolled versus steam-flaked corn in the diet with or without the addition of a culture of Aspergillus oryzae on the performance of high producing dairy cows during hot summer weather. Thirty-two Holstein cows averaging 92 (+/- 60) d in milk were fed a pretreatment diet for 21 d followed by a 70-d experimental period in a completely randomized block design with a 2 x 2 factorial arrangement of treatments. Diets were 1) steam-flaked corn plus 3 g/d of A. oryzae, 2) steam-flaked corn, 3) steam-rolled corn plus 3 g/d of A. oryzae, and 4) steam-rolled corn. Intake was not affected significantly by grain processing or addition of A. oryzae. Compared with effects from steam-rolled corn in the diet, steam-flaked corn increased milk production; percentage of milk protein; yields of milk protein, lactose, and SNF; and the efficiency of conversion of dry matter to fat-corrected milk. Addition of A. oryzae tended to increase protein percentage and increased the percentage of SNF. Changes in body weight and body condition score tended to be higher, and somatic cell count tended to be lower, for cows fed the flaked corn than for cows fed the rolled corn. No interactions were significant. Treatments did not affect rectal temperatures or respiration rates; however, high mean values measured at 1400 h once weekly indicated thermal stress. These data show improved milk production from cows fed steam-flaked corn but not from those fed diets supplemented with A. oryzae.     

Effects of rumen-undegradable protein and feed intake on nitrogen balance and milk protein production in dairy cows

An experiment was designed to determine the response of milk protein production and N utilization in dairy cows to supplementation of a predominantly rumen-undegradable protein (RUP) mixture with a fixed amino acid (AA) pattern and the response to the amount of feed intake. The experiment was designed as a 6 x 6 Latin square with a 3 x 2 factorial arrangement of treatments. The factors were three concentrations of RUP supplement (4.5, 14.9, and 29.1% of dry matter intake) and two levels of feed intake restriction (10 and 20%) of the basal diet. The supplement was designed to approximate a postruminal AA pattern that was similar to bovine caseins for Met, Lys, Phe, His, and Thr. Measurements were made during the last 5 d of each 21-d period. Milk protein production responded linearly as the concentration of RUP supplement in the treatment diet increased within the given range. The difference in feed intake restriction did not affect milk protein production. Efficiency of N utilization for milk production exceeded 30% for cows fed the lowest RUP supplement. Results indicated that there is an opportunity to increase milk protein production by using RUP formulations that are balanced for AA while minimizing waste N excretion.     

Influence of processed corn grain in diets of dairy cows on digestion of nutrients and milk composition

Five primiparous Holstein cows (55 d in milk) that were fitted with ruminal and duodenal cannulas were used in a 4 x 5 incomplete Latin square to determine the effects of blends of steam-flaked and dry-rolled corn on site and extent of nutrient digestion and milk yield and composition. Diets were fed as total mixed rations and consisted of 45% forage and 55% concentrate; each diet contained 27% corn grain. Dietary treatments were composed of blends of dry-rolled and steam-flaked corn in ratios of 100:0, 67:33, 33:67, and 0:100. Intake of dry matter; digestibilities of dry matter, organic matter, acid detergent fiber, cellulose, neutral detergent fiber, fatty acids, and N; and microbial efficiency were unaffected by diet. Ruminal, postruminal, and total tract digestion of starch increased linearly, and starch passage to the duodenum decreased linearly, as the proportion of dry-rolled corn in the diet decreased. Ruminal propionate and valerate increased linearly, and acetate, butyrate, isovalerate, and the acetate to propionate ratio decreased linearly, as proportions of dry-rolled corn in the diet decreased; however, no changes in total volatile fatty acid concentrations in ruminal fluid were observed. Ruminal fluid pH was similar across diets. A decrease in dry-rolled corn decreased ruminal ammonia N and plasma urea N linearly. Milk yield and composition, as well as milk N fractions, were similar across diets. Although changes in fatty acid composition of milk fat were small, linear decreases in percentages of trans-C16:1 and cis-9- and cis-10-C18:1, as well as a linear increase in the percentage of C18:2 occurred as the proportion of dry-rolled corn in the diet decreased. An increased proportion of dry-rolled corn in the diet decreased digestion of starch in the rumen, and patterns of volatile fatty acid concentrations shifted accordingly. However, no effects on lactational parameters were observed.     

Effect of amount of dietary supplement and source of protein on milk production, ruminal fermentation, and nutrient flows in dairy cows

An experiment with a multiple Latin square design using 36 midlactation Friesian cows was carried out to determine the effect of fish meal or soybean meal in a supplement fed at two amounts (3.5 or 7 kg/d) with grass silage. The diets were also fed to four ruminally and duodenally cannulated cows to determine digestibility, ruminal fermentation, and nutrient flows. Dry matter intake, milk production, milk constituent yields, and concentrations of protein and lactose were significantly increased by the supplements when provided at 7 kg/d. The supplement containing fish meal increased milk production, protein and lactose yields, and milk protein concentration, but the increases were smaller than those obtained by increasing the amount of supplement fed. Neither the type nor the amount of supplement fed affected digestibility of dry matter or organic matter, and few significant effects on ruminal fermentation were observed. Supplements fed at the higher allowance significantly increased the flow of dry matter, organic matter, all nitrogenous components, and amino acids to the duodenum. The supplement containing fish meal significantly increased the duodenal flow of nonammonia nonmicrobial N and some amino acids. Results indicated that an increase in the concentration of supplement in the diet is more effective in increasing milk production, protein concentration and yield, and flow of amino acids to the duodenum than is increasing the concentration of ruminally undegradable protein in the supplement.     

Impact of social disorganization on behavior, milk yield, and body weight of dairy cows

To measure the effect of intergroup transfer, four cows were added to a group of 20 cows at 28-day intervals in five trials. The two groups of cows were housed in adjoining lots and fed identical rations from opposite sides of a feed bunk which provided .9 m linear feeding space per cow. Means of the number of agonistic encounters per transferred cow were 9.4, 4.4, 4.3, 3.8, 3.3, and 2.6 during the 1-h period immediately following the first feeding on days 1, 2, 7, 14, 21, and 28. The average dominance values of the transferred cows during the 28 days (.19, .14, and .32) showed no perceptible trend, and mean body weight (567, 569, and 571 kg) was not limited. On day 1 the mean 4% fat-corrected milk yield of the transferred cows decreased .51 kg or 3%, and nontransferred cows increased .01 kg. The decrease in milk yield of the transferred cows was not associated with number of agonistic encounters per se. After day 1 regrouping had no effect on milk yield.     

Loss in milk yield and related composition changes resulting from clinical mastitis in dairy cows

Relationships between clinical mastitis and milk yield and composition in dairy cows were reviewed. Requirements for inclusion were: data had to be collected after 1975; dependent variables for milk yield and milk composition had to be defined at the cow level; mastitis cases had to be defined by clinical signs, and at least 250 lactations had to be considered. Twenty studies dealing with milk-yield loss and, for four of them, also with related composition changes were selected. Study populations, design of data collection, and analysis methods differed widely between studies. As expected from these differences--but also due to structural-variation factors such as type of pathogen, animal and production level--estimates for milk-yield losses differed in average magnitude and pattern. Literature data regarding changes in milk-fat % and total-protein % changes were contradictory. Critiques of materials and methods allowed us to propose some final recommendations for values to be used as basic inputs in economic calculations of losses caused by mastitis.     

The influence of dietary protein intake on milk production and blood composition of high-yielding dairy cows

Isolated rat adipocytes were incubated with serum lipoproteins or lymphchylomicrons which contained 14c-labeled cholesterol. The specific activity of lipoprotein free cholesterol decreased and that of cellular free cholesterol increased linearly up to 7 h. At this time the cell cholesterol specific activity was only 11% of that of medium cholesterol indicating that the rate of exchange was slow. The specific activity of lipoprotein esterified cholesterol remained unchanged while that of cells showed a slight increase suggesting esterification of incorporated free cholesterol. No detectable change of the lipoprotein or cellular cholesterol concentration occurred indicating that the uptake of radioactive free cholesterol was due to exchange without net movement of sterol. The radioactive cholesterol was incorporated into both membrane fraction and the fat droplet of the adipocytes. The rate of cholesterol exchange was temperature-dependent but it was not influenced by the metabolic state of the cells and not by addition of metabolic inhibitors. Trypsin or pronase treatment of the cells were without influence on the rate of the exchange and denaturation of the plasma lipoproteins with formalin increased the rate of exchange. These results indicate that the exchange of cholesterol is a physical chemical process, which is not linked to energy metabolism of the cells, and which is not mediated by either specific lipoprotein receptors on fat cell membranes or pinocytic uptake of lipoproteins. The rate of free cholesterol exchange showed a linear correlation with the concentration of lipoprotein particles in the medium. The relative transfer rate was highest for chylomicrons and decreased in order chylomicron remnants greater than very low density lipoprotein greater than low density lipoprotein greater than high density lipoprotein. A saturation of the system could be obtained only with high density lipoprotein.