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1.
The acyl and Alk-1-enyl groups of the major phosphoglycerides from ox brain myelin and mouse brain microsomal,mitochondrial and myelin fractions 总被引:1,自引:0,他引:1
The major phosphoglycerides from ox brain myelin and mouse brain microsomal, mitochondrial and myelin fractions were separated
by preparative thin layer chromatography. Alk-1-enyl groups from the alk-1-enyl acyl glycerophosphorylethanolamines were reacted
with 1,3-propanediol to form the 1,3-dioxolane derivatives. Acyl groups were converted to the methyl ester derivatives and
the acyl groups from alk-1-enyl acyl glycerophosphorylethanolamines and diacyl glycerophosphorylethanolamines were also determined
separately. The acyl and alk-1-enyl group compositions of the phosphoglycerides from microsomal and mitochondrial fractions
were quite similar. The ethanolamine and serine phosphoglycerides contained large amounts of 18∶0, 18∶1, 20∶4 and 22∶6 acyl
groups. The choline phosphoglycerides had small amounts of polyunsaturated acyl groups and large amounts of 16∶0, 18∶1 and
18∶0 acyl groups. The mitochondrial cardiolipins contained unusual amounts of several acyl groups including 18∶1, 52%; 18∶2,
6%; and 16.1, 4%. A large portion of the mouse brain 18∶2 is in that fraction. The myelin phosphoglycerides were deficient
in saturated and 22∶6 groups and markedly enriched in 18∶1 and 20∶1 groups when compared with the corresponding microsomal
or mitochondrial phosphoglycerides.
Presented in part at the AOCS Meetings, New York, October 1968 and San Francisco, April 1969. 相似文献
2.
The lipid composition of Yoshida ascites hepatoma cells was analyzed together with that of ascitic plasma and of livers and
blood plasma from host and normal rats. In comparison to normal livers, host livers showed no significant differences in the
content of the various lipid classes, but contained a higher percentage of palmitic acid and a lower proportion of arachidonic
acid in the major phospholipid classes. In addition, tumor growth induced a marked hypertriglyceridemia in host animals; changes
in the concentration of other plasma lipid classes were not statistically significant. The ascitic plasma contained small
amounts of lipids mainly constituted by cholesteryl esters and phospholipids. Yoshida hepatoma cells contained less phospholipids
in comparison to both host and normal liver, while the increased level of triglycerides and the decrease of free fatty acids
were not statistically significant. Hepatoma cells showed appreciable amounts of ether-linked lipids associated in part to
neutral lipids (as glyceryl ether diesters) and, in part, to ethanolamine and choline phosphoglycerides. The alkyl groups
in GEDE as well as in ethanolamine and choline phosphoglycerides were mainly constituted by C16∶0 and C18∶0 followed by C18∶1. The alk-1-enyl groups in ethanolamine and choline phosphoglycerides were C16∶0 and C18∶0 with only a minor proportion of C18∶1. In comparison to both host and normal liver, Yoshida hepatoma cells showed significant changes in the fatty acid composition
of neutral lipids and phospholipids. Some of the major changes consisted of an increase of monoenoic acids associated with
a decrease of arachidonic and docosahexaenoic acids in phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol. 相似文献
3.
Effects of parenteral nutrition with high doses of linoleate on the developing human liver and brain
The developmental changes in the fatty acid composition of ethanolamine phosphoglycerides (EPG) and choline phosphoglycerides
(CPG) were studied in the liver and brain of 18 newborn infants with gestational ages ranging from 20 to 44 wk. A small group
of five newborns receiving total parenteral nutrition (TPN) with high doses of linoleic acid (18∶2ω6) was also studied and
compared to controls of the same gestational age to look for effects on the developmental fatty acid patterns of liver and
brain EPG and CPG. TPN with Intralipid 20% was given for 4–12 days, the total fat intake being 14.7–90 g (mean ±S.D.=47.1±29.8
g). The main developmental changes in the liver and brain of the control group were an increase in 22∶6ω3 (docosahexaenoic
acid) at the end of gestation and a linear decrease in 20∶4ω6 (arachidonic acid) and 18∶1ω9 (oleic acid) in EPG and CPG. A
very good correlation in the percent values of these fatty acids in the brain and liver tissues was obtained. Very significant
changes in the fatty acid composition of liver EPG and CPG could be found in the infants receiving TPN with Intralipidmainly
an increase in 18∶2ω6, a decrease in the linoleate elongation/desaturation to longer members of the series and a decrease
in the 22∶6ω3 levels of liver EPG and CPG. In the brain, only an increase in the 18∶2ω6 value of CPG, not accompanied by any
increase in the longer ω6 fatty acids, could be detected. Possible adverse effects of high doses of 18∶2ω6 on the tissue levels
of long chain polyunsaturated fatty acids (PUFA), especially of 22∶6ω3, are discussed. 相似文献
4.
Grace Y. Sun 《Lipids》1979,14(11):918-924
The phospholipid composition and their acyl group profiles from subcellular fractions of guinea pig adrenal gland and the
same fractions from the cortex and medulla of the bovine gland were compared. The phospholipids of guinea pig adrenal were
enriched in diacyl-glycerophosphocholines (GPC) which comprised over 50% of the total phospholipids, but the proportions of
ethanolamine and choline plasmalogens, sphingomyelin and diacyl-glycerophosphoserine (GPS) were lower in guinea pig adrenals
as compared to the bovine adrenals. In the bovine adrenal, sphingomyelin and diacyl-GPS were enriched in the medulla, whereas
diacyl-glycerophosphoinositol (GPI) were enriched in the cortex. Although lysolecithin was present (up to 4.5%) in the bovine
adrenal, only trace amounts of this lipid were detected in the guinea pig adrenal. Characteristic acyl group profiles were
found associated with each type of the phosphoglycerides in adrenal membranes. However, acyl group profiles of the phosphoglycerides
were not greatly different either between the bovine and guinea pig adrenal or with respect to the type of subcellular membranes
isolated. Diacyl-GPC were enriched in 16∶0 and 18∶1, but also contained considerable amounts of 18∶0, 18∶2 and 20∶4. Diacyl-GPE
were enriched in 18∶0, 18∶1 and 20∶4, while diacyl-GPI, diacyl-GPS, as well as alkenylacyl-GPE, were enriched in 20∶4. The
lysolecithin from bovine adrenal membranes contained mainly 16∶0, 18∶0 and 18∶1 with only trace amounts of the polyunsaturated
fatty acids. Other polyunsaturated fatty acids, such as 22∶4 and 22∶6, are apparently not prominent in the phosphoglycerides
from either the bovine or the guinea pig adrenal gland. 相似文献
5.
A fat-deficient diet was initiated in mice before birth and at different ages during development and maturation. The induction
of an essential fatty acid deficiency in brain was most effective when the deficient diet was initiated prenatally. With increasing
time on the deficient diet, there was an increase in 20∶3(n−9) and a decrease in 20∶4(n−6) in the phosphoglycerides of subcellular
brain fractions. The highest ratio of 20∶3(n−9) to 20∶4(n−6) observed was 1.5 for both diacyl and alkenylacyl glycerophosphorylethanolamines
in the synaptosome-rich fraction from mice on the deficient diet from before birth to 7 months of age. The acyl groups of
brain ethanolamine plasmalogens are quite susceptible to alteration by the fat-deficient diet. Elongated products of 20∶3(n−9),
tentatively identified as 22∶3(n−9) and 22∶5(n−9), also were present in brain during essential fatty acid deficiency. These
fatty acids were preferentially linked to the alkenylacyl glycerophosphorylethanolamines. It further was observed that, even
when the deficient diet was initiated after maturation, similar changes in fatty acid composition occurred in both myelin
and synaptosomerich fractions, but at a slower rate. For mice on the deficient diet from 12–18 months of age, the highest
ratio of 20∶3(n−9) to 20∶4(n−6) was 0.6 for the alkenylacyl glycerophosphorylethanolamines from the myelin. 相似文献
6.
The distribution of phospholipids as well as their fatty acid compositions of rat mammary tissues were examined during pregnant,
lactating, and post-weaning periods. There was no apparent change in phospholipids and their acyl groups during the early
and late pregnant periods. However, tissue phospholipid composition was different during pregnant, early, and late lactating
periods. After parturition, there was a marked increase in the proportion of diacyl-glycerophosphorylcholine in the phospholipids
of mammary tissue, but this proportion decreased gradually during lactation. The decrease in diacyl-glycerophosphorylcholine
during lactation was marked by a corresponding increase in diacyl-glycerophosphorylethanolamine. Although the shorter chain
fatty acids of triglycerides were increased during lactation, only a small proportion of these fatty acids was found in the
phosphoglycerides. Marked changes in acyl group composition of individual phospholipids are found during these different physiological
stages. In general, there was a rapid decrease in 20∶4 and an increase in 18∶2 in the major phosphoglycerides during parturition.
The proportion of 20∶4 in the phosphoglycerides remained low throughout the entire lactating period, while that of 18∶2 continued
to increase 2–3 fold. Most of the changes in acyl group of the phosphoglycerides during lactation returned to normal ca. 10
days after weaning. A possible relationship of the variation of phospholipid and acyl group compositions in mammary tissue
to changes in hormonal pattern during different physiological stages is discussed. 相似文献
7.
In guinea pig heart homogenate, 34% of both choline and ethanolamine phosphoglycerides were in the form of plasmalogens (1-alkenyl,
2-acyl glycerophospholipid). Plasmalogens accounted for 39% of the choline phosphoglycerides and 36% of the ethanolamine phosphoglycerides
in the mitochondrial fraction. Ethanolamine plasmalogen was the major ethanolamine phosphoglyceride (63%) in the guinea pig
heart microsomal fraction. A high arachidonyl content was found in both diacyl and 1-alkenyl, 2-acyl glycerophosphoethanolamine.
The C-2 fatty acyl profiles of the diacyl and 1-alkenyl, 2-acyl choline phosphoglycerides differed considerably from each
other in the homogenate as well as in the subcellular fractions. Significant differences in the C-2 fatty acyl profiles also
were observed in diacyl and 1-alkenyl, 2-acyl ethanolamine phosphoglycerides. Such differences suggest there is no direct
metabolic relationship between the diacyl glycerophosphocholine (-ethanolamine) and its plasmalogen analog. 相似文献
8.
Alterations in lipid composition occur in the retinal pigment epithelium and photoreceptor cells of the Royal College of Surgeons
(RCS) dystrophic rat, a model for inherited retinal degeneration. With respect to lipid composition of nonretinal tissues,
the developmental timing of lipid alterations and the incidence of dystrophy are unknown. We determined the fatty acid composition
in choline phosphoglycerides (ChoGpl) and ethanolamine phosphoglycerides (EtnGpl) in the brain, liver, and retina from dystrophic
RCS rats and from their nondystrophic congenics (controls) at the ages of 3 and 6 wk. At 3 wk, the fatty acid compositions
were specific to individual phospholipid classes without any difference between dystrophic and nondystrophic tissues. In plasma
phospholipids, there was an age-related increase in the relative contents of monounsaturated and n-3 polyunsaturated fatty
acids, with only minor differences between dystrophic and nondystrophic rats. At 6 wk, the fatty acid compositions in ChoGpl
and EtnGpl from dystrophic brain and retina were significantly different from those of nondystrophics. The effect of strain
on developmental changes in brain fatty acid composition was significant for 18∶0 and 22∶6n−3 in EtnGpl and for 16∶0, 18∶0,
18∶1n−9, and 20∶4n−6 in ChoGpl. The brain ChoGpl fatty acid composition in nondystrophic rats was similar at 6 wk to that
of normal rats, and there were almost no postweaning changes in the dystrophics. In retinal phospholipids, the effect of dystrophy
was to increase the 20∶4n−6 content in EtnGpl and to decrease 22∶6n−3 in ChoGpl. The 18∶2n−6 and 22∶6n−3 contents in dystrophic
liver ChoGpl were also significantly affected, while no difference was observed in the EtnGpl fraction. The dystrophy affected
the phospholipid fatty acid developmental changes in a tissue- and class-specific manner. Fatty acid metabolism could be selectively
altered in neural and nonneural tissues of developing dystrophic RCS rats. 相似文献
9.
In this study, the 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine content of human platelets was determined. The distribution of arachidonate among the 1,2-diacyl,
1-O-alkyl-2-acyl, and 1-O-alk-l′-enyl-2-acyl classes of choline- and ethanolamine-containing phosphoglycerides was also assessed. The major platelet
phospholipids were choline-containing phosphoglycerides (38%), ethanolamine-containing phosphoglycerides (25%) and sphingomyelin
(18%), with smaller amounts of phosphatidylserine (11%) and phosphatidylinositol (4%). In addition to the diacyl class, the
choline-linked fraction was found to contain both 1-O-alkyl-2-acyl (10%) and 1-O-alk-l′-enyl-2-acyl (9%) species. The ethanolamine-linked fraction, on the other hand, had an elevated level of the 1-O-alk-l′-enyl-2-acyl (60%) species and a small amount of the 1-O-alkyl-2-acyl component (4%). The major fatty acyl residues found in all classes of the choline and ethanolamine phospholipids
were 16∶0, 18∶0, (Δ9), 18∶2(n−6) and 20∶4(n−6). The 1-O-alk-l and 1-O-alk-l′-enyl fraction of the ethanolamine-linked phospholipids also contained substantial amounts of 22∶4(n−6), 22∶5(n−3)
and 22∶6(n−3) acyl chains. Arachidonate comprised 44% of the acyl residues in thesn-2 position of 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine. Corresponding values for the diacyl and 1-O-alk-l′-enyl-2-acyl species were 23% and 25%, respectively, based on all 20∶4(n−6) being linked to thesn-2 position of all classes. In the ethanolamine-linked phosphoglycerides, arachidonate constituted 60%, 20% and 68% of the
acyl groups in thesn-2 position of the 1,2-diacyl, 1-O-alkyl-2-acyl and 1-O-alk-l′-enyl-2-acyl classes, respectively. The content of 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine appears sufficient to support the synthesis of platelet activating factor by a deacylation-reacylation
pathway in platelets. Our findings also demonstrate that human platelets contain a significant amount of 1-O-alkyl-2-arachidonyl-sn-glycero-3-phosphocholine that could possibly serve as a precursor of both platelet activating factor and bioactive arachidonate
metabolites. 相似文献
10.
Platelet phospholipid and fatty acid composition was determined in nine normal subjects and in 11 patients with primary defects
in platelet function. Two of the patients had thrombasthenia (Glanzmann) and nine had various types of abnormalities in platelet
aggregation and platelet factor 3 availability attributed to impairment of the platelet release reaction. The values observed
for platelet lipids in the normal subjects were similar to those reported by others. Four of the patients with a disturbance
in the platelet release reaction were in the same family and showed the same abnormal pattern of platelet lipid composition.
Phospholipid analysis showed a decrease in the relative amount of phosphatidyl ethanolamine (PE) and an increase in lecithin.
Abnormalities in fatty acids consisted of an increase in the relative amounts of 18∶1ω9, 20∶0 and 20∶1ω9 and a decrease in
the 22∶4ω6+24∶1 fraction. Similar changes in PE and 18∶1ω9 were also observed in another patient with a similar defect in
platelet function. In this patient the relative amount of platelet sphingomyelin was also increased. The platelet lipid composition
in the other six patients and in one normal subject given aspirin was essentially normal. 相似文献
11.
The molecular species compositions of the main diacyl phosphoglyceride classes and ether-linked subclasses from sperm of three
species of fish, sea bass Dicentrarchus labrax, Atlantic salmon Salmo salar and Chinook salmon Onchorhynchus tsawytscha, were determined. The phospholipids from sperm were highly unsaturated, dipolyunsaturated fatty acid (diPUFA) molecular species
comprised 64.6 to 71.8% of phosphatidylserine (PS), 10.1 to 17.4% of phosphatidylethanolamine (PE), and 3.3 to 10.1% of phosphatidylcholine
(PC). In sea bass sperm, di22∶6n-3 phospholipid was the predominant diPUFA molecular species, but in both salmon species 22∶5n-3/22∶6n-3
was also a major constituent of PS. Phospholipids containing 22∶6n-3 dominated in sea bass sperm with 16∶0/22∶6n-3 as a major
component of PC and PE, and 18∶0/22∶6n-3 of PE and PS in addition to di22∶6n-3 in the latter two classes. In contrast, both
salmon species contained much more 20∶5n-3 and less 22∶6n-3 so that saturated/20∶5n-3 and monounsaturated/20∶5n-3 molecular
species were more abundant than the corresponding molecules containing 22∶6n-3. Ether-linked lipids comprised 11.3–36.3% of
choline and ethanolamine phosphoglycerides in each fish species. Molecular species containing 22∶6n-3 were the major components
of 1-O-alkyl-2-acyl-glycerophosphocholine, especially 16∶0a/22∶6n-3 in sea bass and 18∶1a/∶6n-3 in the two salmon species, while
in 1-O-alk-1′-enyl-2-acyl-glycerophosphoethanolamine, 16∶0a/22∶6n-3 was the major component in both salmon and 18∶0a/22∶6n-3 in
sea bass with 18∶1a/22∶6n-3 abundant in all three species. In Atlantic salmon 1-O-alkyl-2-acylglycerophosphoethanolamine comprised 24.6% of ethanolamine glycerophospholipids which were predominantly 16∶0a/22∶6n-3
and 18∶1a/22∶6n-3. Phosphatidylinositol from sperm was dominated by stearoyl/C20 PUFA molecular species, in sea bass overwhelmingly 18∶0/20∶4n-6, while in both salmon species 18∶0/20∶4n-6 and 18∶0/20∶5n-3
were equally abundant. 相似文献
12.
The fatty acid composition of testicular lipids has been determined and related to fertility data from groups of dubbed White
Leghorn cockerels after a 50-week feeding period on rations containing 10% safflower oil or coconut oil. Supplements of ethoxyquin
ord-α-tocopherol acetate maintained fertility in birds raised on rations containing safflower oils. This response was associated
with higher proportions of 22∶4 ω6 and lower proportions of 18∶2 ω6 in testicular lipids. Testes size was quite variable in
the unsupplemented group with changes in fatty acid composition being more pronounced in the smaller testes. A multiple regression
was calculated using data from those birds on the safflower oil ration. With a correlation ratio of 0.90 fertility was expressed
as a function of testes size, semen concentration and the proportions of 18∶2 ω6, 20∶4 ω6 and 22∶4 ω6 in testicular lipids.
Despite the low intake of linoleate significant levels of polyunsaturated fatty acids were maintained in testicular lipids
of birds fed the coconut oil rations. The major changes in fatty acid composition of testicular lipids produced by this variable
was a decrease in the proportion of 18∶2 ω6 and an increase in the proportion of 18∶1.
Paper No. 3050, Oregon Agricultural Experiment Station. 相似文献
13.
Edward N. Lambremont 《Lipids》1972,7(8):528-533
When14C-labeled acetate, fatty acids or fatty alcohols were injected into or fed to the tobacco budworm, acyl, alkyl and alk-1-enyl
moieties of the phospholipids incorporated radioactivity. Fatty acids were the principal precursor in acyl bond formation
and fatty alcohols in the synthesis of alkyl and alk-1-enyl glyceryl ethers. Detailed analysis of the etherlinked phosphoglycerides
revealed that most of the radioactivity was in the ethanolamine phosphoglycerides, and very little14C was found in the choline phosphoglycerides. In experiments of a short duration, the alkyl glyceryl ethers incorporated more
radioactivity than the alk-1-enyl glyceryl ethers. The reverse was found with long term experiments, when the alk-1-enyl ethers
had higher radioactivity. In addition to demonstrating the synthesis of ether-linked ethanolamine phosphoglycerides, the data
suggested that fatty alcohols and acids were interconverted by insects and that the alk-1-enyl ethers were derived from the
alkyl ethers.
Presented at the AOCS Meeting, Houston, May 1971.
The following abbreviations and terminology will be used: PE, PC, PI and PS for the generic terms ethanolamine, choline, inositol
and serine phosphoglycerides, respectfully. Alkyl glyceryl ether for 1-alkyl-2-acyl-sn-glycerol-3-phosphoryl-, and alk-1-enyl glyceryl ether for 1-alk-1′-enyl-2-acyl-sn-glycerol-3-phosphoryl-(commonly called plasmalogen). These are adapted from the tentative rules published inJ. Lipid Res. 8:522–528 (1967). 相似文献
14.
The lipid composition of the yellow clam,Mesodesma mactroides, that lives in the northern beaches of the Buenos Aires province of Argentina was studied. The main nonpolar lipids are triglycerides
and alkoxyglycerides. Phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl serine are the main phospholipids.
The predominant fatty acids are 16∶0, 16∶1ω7, 18∶0, 18∶1ω9, 20∶5ω3, and 22∶6ω3. The are mainly provided by the clam's food
and stored in the hepatopancreas. The content of polyunsaturated acids increases in summer together with an increase in nonpolar
lipids and is correlative with an increase in phytoplankton in the sea water. Sexual maturity modifies the lipid composition
of gametes. 相似文献
15.
Manuela Martinez 《Lipids》1989,24(4):261-265
The fatty acid composition of red blood cells, fibroblasts, forebrain, liver and kidney were studied in a 3-month-old infant
who died from Zellweger Syndrome, and the results were compared with those of age-matched controls. Besides a typical increase
in the very long chain fatty acids 26∶0 and 26∶1 and a great reduction in the plasmalogen levels, confirming the diagnosis
of Zellweger Syndrome, some striking changes in the polyunsaturated fatty acid patterns were discovered. The most important
was a very drastic decrease in the values of 22∶6ω3 and 22∶5ω6, the two products of Δ4-desaturation. In the kidney, the level
of 22∶6ω3 fell below that of 26∶0. Consequently, the ratio 26∶0/22∶6ω3 (and 26∶1/22∶6ω3) was most useful in emphasizing the
fatty acid anomalies, especially in renal tissue, where the 26∶0/22∶6ω3 ratio increased to almost 200 times the normal values.
Other significant, although less consistent fatty acid alterations were increases in 18∶2ω6, 18∶3ω6, 20∶3ω6, 18∶4ω3 and 20∶4ω3,
and a decrease in 20∶4ω6 in some tissues. The existence is proposed of a new enzyme defect in peroxisomal disorders, involving
the desaturase system of long chain polyunsaturated fatty acids. 相似文献
16.
The effects of the mixedcis- 18∶1 isomers and mixedtrans-18∶1 isomers present in partially hydrogenated soybean oil (PHSO) upon the patterns of polyunsaturated fatty acids (PUFA)
in liver phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were studied in rats fed concentrates ofcis- 18∶1 ortrans- 18∶1 isomers isolated as triacylglycerides from PHSO. Thecis- 18∶1 andtrans- 18∶1 concentrates were fed at levels equal to those present in PHSO fed at 17.9% of the diet. All diets contained the required
amounts of both linoleic and linolenic acids. Thetrans- 18∶1 concentrate was found to suppress the levels of 20∶4ω6 and 20∶3ω9, and to increase the levels of 18∶2ω6 and 20∶5ω3
in PC and PE. Thecis- 18∶1 concentrate suppressed 20∶4ω6 in PC, 20∶5ω3 in PC and PE, and 18∶2ω6 was more effective than thetrans concentrate in suppressing 22∶6ω3. Thetrans- 18∶1 concentrate was more effective in suppressing 20∶4ω6. Thetrans-18∶ isomers appear to modify PUFA metabolism by inhibition of PUFA synthesis, whereas thecis- 18∶1isomers appear to compete with 2-position fatty acyl transfer and to inhibit ω3 PUFA acylation. 相似文献
17.
In view of the findings that ω3 fatty acids inhibit the synthesis of prostaglandins (PG) from arachidonic acid (20∶4ω6) and
that among immunologically active cells, the macrophage, is a major producer of PG, we undertook a study of the effect of
dietary α-linolenic acid (18∶3ω3) on PG synthesis in the macrophage. Rats were fed purified diets containing either 10% corn
oil (CO) or linseed oil (LO), providing either a low (1/32) or high (3.5/1) ratio of 18∶3ω3 to 18∶2ω6, respectively, for 6
weeks. Fatty acid analysis of macrophage phospholipids showed that there was an appreciable increase in the percentage of
ω3 fatty acids and a decrease in the ω6 fatty acids in macrophages from rats fed the LO diet. The changes in fatty acid composition
were associated with a significant decrease in the synthesis of prostaglandin E (PGE) by macrophages from rats fed the LO
diet. Macrophages from rats fed the 2 dietary, oils did not differ in their ability to degrade PG, thus the difference in
PG production appeared to be a consequence of decreased synthesis only. The dietarily induced changes in PGE synthesis were
readily overcome in vitro by culturing macrophages with complexes of fat-free bovine serum albumin and either 20∶4ω6 or 20∶5ω3.
Part of a dissertation submitted by Linda J. Magrum in partial fulfillment of the requirements for the Ph.D. degree in Nutritional
Sciences.
Honored Student Presentation at the AOCS 74th Annual Meeting, Chicago, 1983. 相似文献
18.
Boars fed diets containing rapeseed oil for 8 weeks showed significantly higher levels of neutral lipids and similar levels
of phospholipids, compared to those fed corn oil. Erucic and eicosenoic acids were found to be high in ethanolamine phosphoglycerides,
and in particular alkenyl acyl-ethanolamine phosphoglyceride. Furthermore, both long chain monoenes were incorporated preferentially
in position 2 of the choline and ethanolamine phosphoglycerides. The alkenyl group composition of the cardiac lipids of pigs
was influenced by dietary fatty acids. When rapeseed oil was fed, small amounts of 20∶1 and 22∶1 alkenyl constituents were
detected.
Contribution No. 641 Animal Research Institute 相似文献
19.
The de novo synthesis of choline and ethanolamine phosphoglycerides in brain microsomes from 18 month-old male rats was investigated
in vitro by using labeled cytidine-5′-diphosphate choline and cytidine-5′-diphosphate ethanolamine as lipid precursors. The
rate of synthesis of the two phospholipid classes was found to be noticeably decreased, as compared to that of adult animals.
The addition of exogenous diacyl glycerols to microsomes from ageing rat brain brings the rate of synthesis nearly to the
adult levels. The synthesis of choline and ethanolamine phosphoglycerides is not affected in the liver microsomes of ageing
rats. The molar distribution of fatty acids in brain microsomal diacyl glycerols of ageing rats is noticeably different from
that of adult animals. The content of monoenoic and dienoic species is increased, whereas that of the tetraenoic species is
decreased. Base exchange reaction for choline and ethanolamine incorporation into respective phospholipids is not affected
in the brain microsomes of the aged rats. 相似文献
20.
An analysis of the phospholipid, cholesterol, and phospholipid fatty acyl composition of isolated plasma membranes of 3T3
and SV3T3 mouse embryo cells has been performed. The results show that the plasma membrane of SV3T3 cells contain relatively
less phosphatidylethanolamine and sphingomyelin and more cholesterol than 3T3 plasma membranes. The fatty acyl composition
of individual phospholipid classes as determined by gas liquid chromatography also showed differences between 3T3 and SV3T3
plasma membranes. The plasma membranes of SV40 transformed 3T3 cells contain: (a) a higher percentage of 18∶1 and less 20∶3
and 20∶4 in phosphatidylethanolamine; (b) a higher percentage of 18∶1 in phosphatidylserine; and (c) a higher percentage of
18∶2 and 20∶4 in phosphatidylinositol. 相似文献