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碱-酶法提取啤酒酵母粉中β-(1,3)-D-葡聚糖 总被引:1,自引:0,他引:1
以啤酒酵母粉为原料,综合考虑提取率、蛋白质含量和多糖含量3个指标,在单因素实验的基础上,采用正交实验,得出碱-酶法提取废啤酒酵母中β-(1,3)-D-葡聚糖的理想工艺条件:酵母粉经过水洗,脱色后,按照150mL∶10g的比例加入3%的NaOH溶液,在80℃水浴条件下水解2h,离心,洗涤,调整pH至8.5~9.0,再加入600U/g碱性蛋白酶(以干酵母粉计),在55℃下水解24h,离心,沉淀,干燥得到成品。β-(1,3)-D-葡聚糖的提取率为13.8%,产品多糖含量85.2%、蛋白质含量1.2%、水分9.2%,产品为乳白色粉状固体,色度为L值83.07、a值2.12、b值8.86。 相似文献
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自溶-碱法提取啤酒酵母β-(1,3)-D葡聚糖工艺研究 总被引:1,自引:0,他引:1
通过单因素和正交实验得出自溶-碱法提取啤酒酵母β-(1,3)-D葡聚糖的最佳工艺条件为:定量酵母粉,以料液比1∶30加入3%NaCl水溶液,40℃水浴24h;4000r/min离心去上清,以料液比1∶30加入2%NaOH水溶液,95℃水浴提取3h,4000r/min离心去上清,蒸馏水洗两次,60℃烘干,得成品。 相似文献
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以啤酒酵母为原料提取β-葡聚糖,建立了一种气相色谱测定β-葡聚糖和甘露聚糖(副产物)含量的方法,并与日本ADEKA株式会社的β-葡聚糖产品中相应多糖含量进行了对比。样品用三氟乙酸(TFA)进行水解后,对水解单糖进行乙酰化,制备成糖腈乙酰酯衍生物,然后采用中极性柱进行气相色谱分析,最后以外标法定量测定。结果表明,β-葡聚糖和甘露聚糖的单糖成分经乙酰化后能得到较好的色谱分离效果,本实验制备的产品中β-葡聚糖和甘露聚糖的含量分别为32.39%和16.82%,而日本产品中对应多糖含量则分别为30.63%和16.13%。方法的相对标准偏差分别为5.09%(β-葡聚糖)和6.25%(甘露聚糖)(n=3),回收率在87.14%~101.2%,此法可准确测定β-葡聚糖的含量。 相似文献
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以啤酒酵母为原料提取β-葡聚糖,建立了一种气相色谱测定β-葡聚糖和甘露聚糖(副产物)含量的方法,并与日本ADEKA株式会社的β-葡聚糖产品中相应多糖含量进行了对比。样品用三氟乙酸(TFA)进行水解后,对水解单糖进行乙酰化,制备成糖腈乙酰酯衍生物,然后采用中极性柱进行气相色谱分析,最后以外标法定量测定。结果表明,β-葡聚糖和甘露聚糖的单糖成分经乙酰化后能得到较好的色谱分离效果,本实验制备的产品中β-葡聚糖和甘露聚糖的含量分别为32.39%和16.82%,而日本产品中对应多糖含量则分别为30.63%和16.13%。方法的相对标准偏差分别为5.09%(β-葡聚糖)和6.25%(甘露聚糖)(n=3),回收率在87.14%~101.2%,此法可准确测定β-葡聚糖的含量。 相似文献
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谷物β-葡聚糖是β-D-吡喃型葡萄糖基单元通过(1→4)-β-键重复连接并被单一(1→3)-β-D-键分离而形成的一种线性同聚多糖,同时也是谷物水溶性膳食纤维的主要成分,主要存在于大麦、燕麦、青稞、小麦和黑麦中。谷物可溶性β-葡聚糖的生理效应与其独特的结构具有密切关系,本文注重于谷物β-葡聚糖的分子结构特征与其在胃肠道中的生理活性的关系,总结了谷物可溶性β-葡聚糖在降低胆固醇、餐后血糖指数与胰岛素水平上等生理活性的研究,探讨了谷物β-葡聚糖对肠道菌群与免疫作用的新机制。对几种β-葡聚糖生理活性机理的研究进行了描述:增加小肠黏度水平因此延缓胃排空、消化和分子吸收,包括葡萄糖、膳食胆固醇和胆汁酸;于小肠中与胆汁酸相结合降低胆汁酸重吸收,进而促进利用胆固醇的胆汁酸合成;降低餐后血糖指数与胰岛素水平改善胰岛素敏感性;于盲肠、结肠中发酵改善肠道健康。 相似文献
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啤酒废酵母综合破壁法提取酵母味素技术 总被引:1,自引:0,他引:1
本文研究了高压均质、酶法溶胞、冻融法三种细胞壁破碎法对啤酒废酵母抽提物(酵母味素)的得率、蛋白质的转化率及氨基氮溶出率的影响。实验结果,在适宜的自溶条件下,利用综合破壁法可使酵母抽提物中上述三项指标显著提高。 相似文献
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Dispersal of beer on a commercially available tube of kieselguhr and elution with dichloromethane was found to be a much faster and more efficient method of obtaining a beer flavour extract than liquid-liquid extraction. The constitution of each type of extract was qualitatively similar. The use of an internal standard allowed major flavour components to be quantitatively analysed. 相似文献
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Beer foam generated under conditions encountered at dispense was separated from beer, allowed to collapse and sampled at regular intervals. The yield of collapsed beer foam decreased logarithmically with time. Analysis of the composition of collapsed beer foam fractions over a period of time, considered to be relevant to actual consumption of beer (30 minutes) showed that polypeptide material and isomerised α acids were selectively partitioned in beer foam. The concentration of total polypeptides, soluble polypeptides, isomerised α acids and foam precipitate material in collapsed beer foam, firstly increased with foam collapse time and then approached maximal values. Under these conditions the concentration of polypeptide material and isomerised α acids increased 10–30 fold and 7 fold respectively as compared to beer. No evidence was obtained for the preferential partition of purine nucleosides and free bases in foam (adenosine + deoxyadenosine, guanosine + deoxyguanosine, adenine, guanine and xanthine). 相似文献
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啤酒用玉米米工艺检测指标的研究 总被引:1,自引:0,他引:1
通过对啤酒用辅料玉米米的工艺检测指标的研究,从水分、粒度、浸出物、脂肪等几方面制定啤酒用玉米米产品标准。以其为根据,采购原料玉米,验证、修订、完善玉米联产加工工艺(中试)及其参数,选择最佳加工工艺,确定更为合理的工艺参数。 相似文献
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S. A. Halsey 《Journal of the Institute of Brewing》1985,91(5):306-312
Both transmission and transflectance near infrared spectroscopy have been evaluated for the rapid analysis of beer components. Results of sufficient accuracy have been obtained for ethanol content and Original Gravity. Application of a first derivative mathematical treatment on the spectral data improved calibration equations. Better accuracy was achieved using the transmission mode compared to that using the transflectance mode. 相似文献
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食品工业废料生产饲料酵母的研究进展 总被引:1,自引:0,他引:1
论述了以食品工业废液、废渣为原料,采用生物技术生产饲料酵母的生产工艺,并对其营养价值及饲用价值进行了分析。技术经济分析表明:有显著的经济效益和社会效益。 相似文献
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When inoculated into a defined minimal medium, yeast could not absorb all the magnesium, even if this element was limiting. The cells tended to absorb a constant amount of magnesium, ca 1·1 pg per cell, so long as the content of the medium was sufficient, rather than absorb magnesium in proportion to its initial concentration. Calcium competed with magnesium and prevented yeast growth almost entirely when present at a ten times excess. At a two to three times excess the effect was more on the length of the lag phase and mean generation time than on the total amount of growth. 相似文献