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1.
Fu Q Saiz E Rahaman MN Tomsia AP 《Materials science & engineering. C, Materials for biological applications》2011,31(7):1245-1256
The repair and regeneration of large bone defects resulting from disease or trauma remains a significant clinical challenge. Bioactive glass has appealing characteristics as a scaffold material for bone tissue engineering, but the application of glass scaffolds for the repair of load-bearing bone defects is often limited by their low mechanical strength and fracture toughness. This paper provides an overview of recent developments in the fabrication and mechanical properties of bioactive glass scaffolds. The review reveals the fact that mechanical strength is not a real limiting factor in the use of bioactive glass scaffolds for bone repair, an observation not often recognized by most researchers and clinicians. Scaffolds with compressive strengths comparable to those of trabecular and cortical bones have been produced by a variety of methods. The current limitations of bioactive glass scaffolds include their low fracture toughness (low resistance to fracture) and limited mechanical reliability, which have so far received little attention. Future research directions should include the development of strong and tough bioactive glass scaffolds, and their evaluation in unloaded and load-bearing bone defects in animal models. 相似文献
2.
Controlling ion release from bioactive glass foam scaffolds with antibacterial properties 总被引:1,自引:0,他引:1
Jones JR Ehrenfried LM Saravanapavan P Hench LL 《Journal of materials science. Materials in medicine》2006,17(11):989-996
Bioactive glass scaffolds have been produced, which meet many of the criteria for an ideal scaffold for bone tissue engineering
applications, by foaming sol-gel derived bioactive glasses. The scaffolds have a hierarchical pore structure that is very
similar to that of cancellous bone. The degradation products of bioactive glasses have been found to stimulate the genes in
osteoblasts. This effect has been found to be dose dependent. The addition of silver ions to bioactive glasses has also been
investigated to produce glasses with bactericidal properties. This paper discusses how changes in the hierarchical pore structure
affect the dissolution of the glass and therefore its bioactivity and rate of ion delivery and demonstrates that silver containing
bioactive glass foam scaffolds can be synthesised. It was found that the rate of release of Si and Ca ions was more rapid
for pore structures with a larger modal pore diameter, although the effect of tailoring the textural porosity on the rate
of ion release was more pronounced. Bioactive glass scaffolds, containing 2 mol% silver, released silver ions at a rate that
was similar to that which has previously been found to be bactericidal but not high enough to be cytotoxic to bone cells. 相似文献
3.
Local antimicrobial delivery is a potential area of research conceptualized to provide alternative and better methods of treatment for cases, as osteomyelitis where avascular zones prevent the delivery of drugs from conventional routes of administration. Drug-loaded polymers and calcium phosphates as hydroxyapatites have been tried earlier. Bioactive glasses are bone-filling materials used for space management in orthopedic and dental surgery. A new bioactive glass (SSS2) was synthesized and fabricated into porous scaffold with a view to provide prolonged local delivery of gatifloxacin and fluconazole as suitable for the treatment of osteomyelitis. The new SSS2 was characterized by Fourier transform infrared (FTIR) and X-ray diffraction (XRD) analyses. In addition, the bioactivity of the SSS2 glass and resulting scaffold was examined by in vitro acellular method and ascertained by FTIR and XRD. The pore size distribution was analysed by mercury intrusion porosimetry and the release of drugs from scaffolds were studied in vitro. The glass and the resulting scaffolds were bioactive indicating that they can bond with bone in vivo. The scaffolds were porous with pores predominantly in the range of 10-60 μm, released the drugs effectively for 6 weeks and deemed suitable for local delivery of drugs to treat osteomyelitis. 相似文献
4.
Jung-Ju Kim Guang-Zhen Jin Hye-Sun Yu Seong-Jun Choi Hae-Won Kim Ivan B. Wall 《Materials science & engineering. C, Materials for biological applications》2012,32(8):2545-2551
Development of bone scaffolds with excellent osteogenic potential is highly important for stem cell-based bone engineering. Here we developed novel scaffolds made of poly(lactic acid) (PLA) biopolymer with bioactive glass nanocomponent. In vitro bone bioactivity and osteogenic potential of the nanocomposite scaffolds were determined using bone marrow mesenchymal stem cells. Glass nanocomponent was evenly embedded within the PLA matrix while preserving the scaffold pore structure. Simulated body fluid (SBF) test revealed rapid induction of bone mineral-like apatite over the surface of the nanocomposite scaffold, which was not readily observed in the PLA. Cells adhered well onto the nanocomposite scaffold and multiplied during culture period. Nanocomposite scaffold significantly stimulated alkaline phosphatase (ALP) activity and the expression of bone-associated genes (collagen I, ALP, osteopontin and osteocalcin) with respect to PLA. Western blot analysis confirmed the osteogenic protein level was also higher on the nanocomposite scaffold. Results suggest that the nanocomposite scaffolds provide favorable conditions for osteogenesis of MSCs and thus find potential uses in bone tissue engineering. 相似文献
5.
Sheng Yue Peter D. Lee Gowsihan Poologasundarampillai Zhengzhong Yao Peter Rockett Andrea H. Devlin Christopher A. Mitchell Moritz A. Konerding Julian R. Jones 《Journal of materials science. Materials in medicine》2010,21(3):847-853
X-ray microtomography (μCT) is a popular tool for imaging scaffolds designed for tissue engineering applications. The ability
of synchrotron μCT to monitor tissue response and changes in a bioactive glass scaffold ex vivo were assessed. It was possible
to observe the morphology of the bone; soft tissue ingrowth and the calcium distribution within the scaffold. A second aim
was to use two newly developed compression rigs, one designed for use inside a laboratory based μCT machine for continual
monitoring of the pore structure and crack formation and another designed for use in the synchrotron facility. Both rigs allowed
imaging of the failure mechanism while obtaining stress–strain data. Failure mechanisms of the bioactive glass scaffolds were
found not to follow classical predictions for the failure of brittle foams. Compression strengths were found to be 4.5–6 MPa
while maintaining an interconnected pore network suitable for tissue engineering applications. 相似文献
6.
The development of the new technologies of bone tissue engineering requires the production of bioactive and biodegradable macroporous scaffolds. Hydroxyapatite (HA) ceramics are useful bone substitutes, but they degrade minimally. Tricalcium phosphates also show poor ability of Ca-P formation both in-vitro and in-vivo, although they are degradable. The present study introduces a biodegradable, bioactive, and macroporous scaffold with suitable mechanical properties. The prepared hydroxyapatite scaffold was coated with a nanocrystalline bioactive glass layer to be subsequently sintered at different temperatures. The bioactivity and degradability of the coated scaffolds were investigated by standard procedures. The ability to induce Ca-P formation in SBF (simulated body fluid) was also investigated semi-quantitatively. BS1 scaffolds (scaffolds sintered at 800 °C with a holding time of 2 h) showed remarkable bioactivity and degradability simultaneously. Formation of a nanocrystalline phase (Si2PO7) during the sintering considerably decreased the capability of BS1 scaffolds for Ca-P formation and the rate of degradation but enhanced their mechanical properties. The BS1 scaffolds showed not only significant bioactivity but also good degradability and suitable mechanical property. 相似文献
7.
Here we produced macroporous and nanofibrous scaffolds with bioactive nanocomposite composition, poly(lactic acid) (PLA) incorporating bioactive glass nanoparticles (BGnp) up to 30 wt%, targeting bone regeneration. In particular, the nanofibrous structure in the scaffolds was generated by using a bicyclic monoterpene, camphene (C10H16), through a phase-separation process with PLA-BGnp phase in chloroform/1,4-dioxane co-solvent. Furthermore, macropores were produced by the impregnation of salt particles and their subsequent leaching out, followed by freezing and lyophilization processes. The produced PLA-BGnp scaffolds presented highly porous and nanofibrous structure with porosities of 90–95% and pore sizes of over hundreds of micrometers. BGnp with sizes of ∼90 nm were also evenly impregnated within the PLA matrix, featuring a nanocomposite structure. The nanofibrous scaffolds exhibited enhanced hydrophilicity and more rapid hydrolytic degradation as the incorporated BGnp content increased. The bone-bioactivity of the scaffolds was substantially improved with the incorporation of BGnp, exhibiting rapid formation of apatite throughout the scaffolds in a simulated body fluid. The developed macroporous and nanofibrous scaffolds with PLA-BGnp bioactive composition are considered as a novel 3D matrix potentially useful for bone tissue engineering. 相似文献
8.
Xin Liu Zongping Xie Changqing Zhang Haobo Pan Mohamed N. Rahaman Xin Zhang Qiang Fu Wenhai Huang 《Journal of materials science. Materials in medicine》2010,21(2):575-582
The objective of this work was to evaluate borate bioactive glass scaffolds (with a composition in the system Na2O–K2O–MgO–CaO–B2O3–P2O5) as devices for the release of the drug Vancomycin in the treatment of bone infection. A solution of ammonium phosphate,
with or without dissolved Vancomycin, was used to bond borate glass particles into the shape of pellets. The in vitro degradation
of the pellets and their conversion to a hydroxyapatite-type material in a simulated body fluid (SBF) were investigated using
weight loss measurements, chemical analysis, X-ray diffraction, and scanning electron microscopy. The results showed that
greater than 90% of the glass in the scaffolds degraded within 1 week, to form poorly crystallized hydroxyapatite (HA). Pellets
loaded with Vancomycin provided controlled release of the drug over 4 days. Vancomycin-loaded scaffolds were implanted into
the right tibiae of rabbits infected with osteomyelitis. The efficacy of the treatment was assessed using microbiological
examination and histology. The HA formed in the scaffolds in vivo, resulting from the conversion of the glass, served as structure
to support the growth of new bone and blood vessels. The results in this work indicate that bioactive borate glass could provide
a promising biodegradable and bioactive material for use as both a drug delivery system and a scaffold for bone repair. 相似文献
9.
Zhipeng Gu Xu Zhang Li Li Qiguang Wang Xixun Yu Ting Feng 《Materials science & engineering. C, Materials for biological applications》2013,33(1):274-281
The development of suitable bioactive three-dimensional scaffold for the promotion of bone regeneration is critical in bone tissue engineering. The purpose of this study was to investigate in vivo osteogenesis of the porous strontium-doped calcium polyphosphate (SCPP) scaffolds for bone repair, as well as the relationship between osteogenic properties of SCPP scaffolds and the secretion of bFGF and VEGF from osteoblasts stimulated by SCPP. Besides, the advantages of scaffolds seeded with mesenchymal stem cells (MSCs) for bone repair were also studied. Firstly, the bone repair evaluation of scaffolds was performed on a rabbit segmental bony defects model over a period of 16 weeks by histology combined with X-ray microradiography. And then, in order to avoid the influence from the other factors such as hypoxia which emerge in vivo study and affect the secretion of VEGF and bFGF from host cells, human osteoblast-like cells (MG63) were seeded to SCPP, CPP and HA scaffolds in vitro to determine the ability of these scaffolds to stimulate the secretion of angiogenic growth factors (VEGF and bFGF) from MG63 and further explore the reason for the better osteogenic properties of SCPP scaffolds. The histological and X-ray microradiographic results showed that the SCPP scaffolds presented better osteogenic potential than CPP and HA scaffolds, when combined with MSCs, the SCPP scaffolds could further accelerate the bone repair. And the amounts of VEGF measured by ELISA assay in SCPP, CPP and HA groups after cultured for 7 days were about 364.989 pg/mL, 244.035 pg/mL and 232.785 pg/mL, respectively. Accordingly, the amounts of bFGF were about 27.085 pg/mL, 15.727 pg/mL and 8.326 pg/mL. The results revealed that the SCPP scaffolds significantly enhanced the bFGF and VEGF secretion compared with other scaffolds. The results presented in vivo and in vitro study demonstrated that the SCPP could accelerate bone formation through stimulating the secretion of VEGF and bFGF from osteoblasts, making it attractive for bone regeneration. 相似文献
10.
Mehdikhani-Nahrkhalaji M Fathi MH Mortazavi V Mousavi SB Hashemi-Beni B Razavi SM 《Journal of materials science. Materials in medicine》2012,23(2):485-495
This study aimed at preparation and in vitro and in vivo evaluation of novel bioactive, biodegradable, and antibacterial nanocomposite
coating for the improvement of stem cells attachment and antibacterial activity as a candidate for dental implant applications.
Poly (lactide-co-glycolide)/bioactive glass/hydroxyapatite (PBGHA) nanocomposite coating was prepared via solvent casting process. The nanoparticle
amounts of 10, 15, and 20 weight percent (wt%) were chosen in order to determine the optimum amount of nanoparticles suitable
for preparing an uniform coating. Bioactivity and degradation of the coating with an optimum amount of nanoparticles were
evaluated by immersing the prepared samples in simulated body fluid and phosphate buffer saline (PBS), respectively. The effect
of nanocomposite coating on the attachment and viability of human adipose-derived stem cells (hASCs) was investigated. Kirschner
wires (K-wires) of stainless steel were coated with the PBGHA nanocomposite coating, and mechanical stability of the coating
was studied during intramedullary implantation into rabbit tibiae. The results showed that using 10 wt% nanoparticles (5 wt%
HA and 5 wt% BG) in the nanocomposite could provide the desired uniform coating. The study of in vitro bioactivity showed
rapid formation of bone-like apatite on the PBGHA coating. It was degraded considerably after about 60 days of immersion in
PBS. The hASCs showed excellent attachment and viability on the coating. PBGHA coating remained stable on the K-wires with
a minimum of 96% of the original coating mass. It was concluded that PBGHA nanocomposite coating provides an ideal surface
for the stem cells attachment and viability. In addition, it could induce antibacterial activity, simultaneously. 相似文献
11.
Wang S Falk MM Rashad A Saad MM Marques AC Almeida RM Marei MK Jain H 《Journal of materials science. Materials in medicine》2011,22(5):1195-1203
Recently, nano–macro dual-porous, three-dimensional (3D) glass structures were developed for use as bioscaffolds for hard
tissue regeneration, but there have been concerns regarding the interconnectivity and homogeneity of nanopores in the scaffolds,
as well as the cytotoxicity of the environment deep inside due to limited fluid access. Therefore, mercury porosimetry, nitrogen
absorption, and TEM have been used to characterize nanopore network of the scaffolds. In parallel, viability of MG 63 human
osteosarcoma cells seeded on scaffold surface was investigated by fluorescence, confocal and electron microscopy methods.
The results show that cells attach, migrate and penetrate inside the glass scaffold with high proliferation and viability
rate. Additionally, scaffolds were implanted under the skin of a male New Zealand rabbit for in vivo animal test. Initial
observations show the formation of new tissue with blood vessels and collagen fibers deep inside the implanted scaffolds with
no obvious inflammatory reaction. Thus, the new nano–macro dual-porous glass structure could be a promising bioscaffold for
use in regenerative medicine and tissue engineering for bone regeneration. 相似文献
12.
Guojin Hou Fang Zhou Yan Guo Zhongwei Yang Ailing Li Chen Wang Dong Qiu 《Journal of materials science. Materials in medicine》2017,28(11):181
The purpose is to study the in vivo bioactivity of this scaffold and verify its ability to simulate the characteristics of cancellous bone. Twenty-four adult New Zealand white rabbits were divided into three groups. Bone defects above the femoral condylar of both sides were created. A newly designed bioactive nanoparticle–gelatin composite scaffold was implanted to the experimental side, while the control side was left without implantation. The repair of bone defect was monitored by X-ray examination, gross observation, Micro-CT examination and histological observation of the area of bone defect 4, 8 and 12 weeks after surgery. There was void of new bone tissue in medullary cavity in the bone defect area of the control side. In the experimental side, the composite scaffold displayed excellent biodegradability, bioactivity and cyto-compatibility. With the time laps, new bone tissue grew from the edge to center as revealed by both Micro-CT image and staining biopsy, which complies with the “creeping substitution” process. The mechanical properties of the newly designed bioactive nanoparticle–gelatin composite scaffold and the 3-D structure of new bone tissue are comparable to the surrounding cancellous bones. This newly developed bioactive nanoparticle–gelatin composite scaffold possesses good biocompatibility and in vivo osteogenic capability for bone defect repair. It may be a promising artificial bone grafts. 相似文献
13.
《Drug development and industrial pharmacy》2013,39(5):538-546
Local antimicrobial delivery is a potential area of research conceptualized to provide alternative and better methods of treatment for cases, as osteomyelitis where avascular zones prevent the delivery of drugs from conventional routes of administration. Drug-loaded polymers and calcium phosphates as hydroxyapatites have been tried earlier. Bioactive glasses are bone-filling materials used for space management in orthopedic and dental surgery. A new bioactive glass (SSS2) was synthesized and fabricated into porous scaffold with a view to provide prolonged local delivery of gatifloxacin and fluconazole as suitable for the treatment of osteomyelitis. The new SSS2 was characterized by Fourier transform infrared (FTIR) and X-ray diffraction (XRD) analyses. In addition, the bioactivity of the SSS2 glass and resulting scaffold was examined by in vitro acellular method and ascertained by FTIR and XRD. The pore size distribution was analysed by mercury intrusion porosimetry and the release of drugs from scaffolds were studied in vitro. The glass and the resulting scaffolds were bioactive indicating that they can bond with bone in vivo. The scaffolds were porous with pores predominantly in the range of 10–60 µm, released the drugs effectively for 6 weeks and deemed suitable for local delivery of drugs to treat osteomyelitis. 相似文献
14.
Bioactive borosilicate glass scaffolds: improvement on the strength of glass-based scaffolds for tissue engineering 总被引:1,自引:0,他引:1
Liu X Huang W Fu H Yao A Wang D Pan H Lu WW 《Journal of materials science. Materials in medicine》2009,20(1):365-372
Three-dimensional macroporous scaffolds with the pore size of 200-500 mum were fabricated by replication method using bioactive borosilicate glass from Na(2)O-K(2)O-MgO-CaO-SiO(2)-P(2)O(5)-B(2)O(3) system. The effects of the strength of the strut in reticulated scaffold, as well as the geometrical parameter of the scaffold on the strength of reticulated scaffold were investigated. Scanning electron microscope (SEM) and X-ray diffraction (XRD) results show that the solidified glass struts in the reticulated scaffold could be obtained through a sufficient vicious flow of glass, during the fabrication. By increasing the solid content in slurries, from which the scaffold was made, the load-bearing units of the reticulated scaffold switch from struts to the walls between the pores, and the compressive strength dramatically climbs higher than the theoretical strength calculated by Gibson model. In particular, the compressive strength of the reticulated scaffold, as high as approximately 10 MPa with the porosity of approximately 70%, is close to the reported compressive values of human cancellous bone. This indicates the bioactive borosilicate glass-based scaffold is a promising candidate for bone tissue engineering. 相似文献
15.
M. Diba M. Kharaziha M.H. Fathi M. Gholipourmalekabadi A. Samadikuchaksaraei 《Composites Science and Technology》2012
Biocomposite scaffolds made from polymers and bioceramics can provide the mechanical structure necessary for osteoinductivity in the growth of new bone. The aim of this research was to investigate the properties of a novel nanocomposite scaffold made from a combination of polycaprolactone (PCL) and forsterite nanopowder which could find use in bone tissue engineering applications. The scaffold itself was fabricated by a method of solvent casting and particle leaching. The effect of forsterite content on the mechanical properties, bioactivity, biodegradability, and cytotoxicity of the scaffolds was investigated. Significant improvement in the mechanical properties was observed in the nanocomposite scaffolds as compared to that seen in the pure PCL scaffolds. Bioactivity was also observed in the nanocomposite scaffolds, a trait which was not present in the pure PCL scaffolds. Biodegradation assay indicated that the addition of forsterite nanopowder could modulate the degradation rate of PCL. In vitro tests of cytotoxicity and osteoblast proliferation showed that the nanocomposite scaffolds were non-cytotoxic, thereby allowing cells to adhere, grow, and proliferate on the surface of these scaffolds. The results obtained in this experiment suggest that the combination of PCL with forsterite nanopowder can be used to form scaffolds suitable for use in bone tissue engineering. The exact material behavior required can be adjusted through variation of the ratio between PCL and forsterite nanopowder used to form the scaffold. 相似文献
16.
Gautam Gupta Ali Zbib Ahmed El-Ghannam Marwan Khraisheh Hussein Zbib 《Composite Structures》2005,71(3-4):423-428
A novel bioactive, porous silica–calcium phosphate nanocomposite (SCPC) that can be used to treat large bone defects in load-bearing positions has been tested and has shown great potential for applications in tissue engineering. Porosity is essential to the performance of the composite material as a tissue engineering scaffold, as porous scaffolds provide a physical, 3-D template to support new tissue formation. However, porosity characterization using conventional techniques such as porosimetry or scanning electron microscopy requires extensive preparation of samples and may destroy important features during preparation and analysis stage. In this work, the new composite is characterized using an advanced high resolution X-ray computed tomography, which is a non-destructive testing technique that allows construction of the 3-D topology of the microstructure. The results clearly show the effectiveness and versatility of this technique in characterizing the porous architecture of the novel composite biomaterial. The pore distribution, morphology and interconnectivity in the composite scaffolds were found to be ideal for use in tissue engineering applications. 相似文献
17.
Quanying Liu Manyu Chen Peiyang Gu Lei Tong Peilei Wang Jiayi Zhu Yang Xu Gonggong Lu En Luo Jie Liang Yujiang Fan Xingdong Zhang Yong Sun 《Small (Weinheim an der Bergstrasse, Germany)》2023,19(19):2206960
Integrating a biomimetic extracellular matrix to improve the microenvironment of 3D printing scaffolds is an emerging strategy for bone substitute design. Here, a “soft–hard” bone implant (BM-g-DPCL) consisting of a bioactive matrix chemically integrated on a polydopamine (PDA)-coated porous gradient scaffold by polyphenol groups is constructed. The PDA-coated “hard” scaffolds promoted Ca2+ chelation and mineral deposition; the “soft” bioactive matrix is beneficial to the migration, proliferation, and osteogenic differentiation of stem cells in vitro, accelerated endogenous stem cell recruitment, and initiated rapid angiogenesis in vivo. The results of the rabbit cranial defect model (Φ = 10 mm) confirmed that BM-g-DPCL promoted the integration between bone tissue and implant and induced the deposition of bone matrix. Proteomics confirmed that cytokine adhesion, biomineralization, rapid vascularization, and extracellular matrix formation are major factors that accelerate bone defect healing. This strategy of highly chemically bonded soft–hard components guided the construction of the bioactive regenerative scaffold. 相似文献
18.
H. Lu T. Zhang X. P. Wang Q. F. Fang 《Journal of materials science. Materials in medicine》2009,20(3):793-798
Submicron bioactive glass fibers 70S30C (70 mol% SiO2, 30 mol% CaO) acting as bone tissue scaffolds were fabricated by electrospinning method. The scaffold is a hierarchical pore
network that consists of interconnected fibers with macropores and mesopores. The structure, morphological characterization
and mechanical properties of the submicron bioactive glass fibers were studied by XRD, EDS, FIIR, SEM, N2 gas absorption analyses and nanoindentation. The effect of the voltage on the morphology of electrospun bioactive glass fibers
was investigated. It was found that decreasing the applied voltage from 19 to 7 kV can facilitate the formation of finer fibers
with fewer bead defects. The hardness and Young’s modulus of submicron bioactive glass fibers were measured as 0.21 and 5.5 GPa,
respectively. Comparing with other bone tissue scaffolds measured by nanoindentation, the elastic modulus of the present scaffold
was relatively high and close to the bone. 相似文献
19.
Huang J Lin YW Fu XW Best SM Brooks RA Rushton N Bonfield W 《Journal of materials science. Materials in medicine》2007,18(11):2151-2157
Nano-sized hydroxyapatite (nanoHA) reinforced composites, mimicking natural bone, were produced. Examination by transmission
electron microscopy revealed that the nanoHA particles had a rod-like morphology, 20–30 nm in width and 50–80 nm in length.
The phase composition of hydroxyapatite was confirmed by X-ray diffraction. The nanoHA particles were incorporated into poly-2-hydroxyethylmethacrylate
(PHEMA)/polycaprolactone (PCL) matrix to make new nanocomposites: nanoHA-PHEMA/PCL. Porous nanocomposite scaffolds were then
produced using a porogen leaching method. The interconnectivity of the porous structure of the scaffolds was revealed by non-destructive
X-ray microtomography. Porosity of 84% was achieved and pore sizes were approximately around 300–400 μm. An in vitro study
found that the nanocomposites were bioactive as indicated by the formation of a bone-like apatite layer after immersion in
simulated body fluid. Furthermore, the nanocomposites were able to support the growth and proliferation of primary human osteoblast
(HOB) cells. HOB cells developed a well organized actin cytoskeletal protein on the nanocomposite surface. The results demonstrate
the potential of the nanocomposite scaffolds for tissue engineering applications for bone repair. 相似文献
20.
Robin Augustine Pan Dan Alejandro Sosnik Nandakumar Kalarikkal Nguyen Tran Brice Vincent Sabu Thomas Patrick Menu Didier Rouxel 《Nano Research》2017,10(10):3358-3376
Piezoelectric materials that generate electrical signals in response to mechanical strain can be used in tissue engineering to stimulate cell proliferation.Poly (vinylidene fluoride-trifluoroethylene) (P(VDF-TrFE)),a piezoelectric polymer,is widely used in biomaterial applications.We hypothesized that incorporation of zinc oxide (ZnO) nanoparticles into the P(VDF-TrFE) matrix could promote adhesion,migration,and proliferation of cells,as well as blood vessel formation (angiogenesis).In this study,we fabricated and comprehensively characterized a novel electrospun P(VDF-TrFE)/ZnO nanocomposite tissue engineering scaffold.We analyzed the morphological features of the polymeric matrix by scanning electron microscopy,and utilized Fourier transform infrared spectroscopy,X-ray diffraction,and differential scanning calorimetry to examine changes in the crystalline phases of the copolymer due to addition of the nanoparticles.We detected no or minimal adverse effects of the biomaterials with regard to blood compatibility in vitro,biocompatibility,and cytotoxicity,indicating that P(VDF-TrFE)/ZnO nanocomposite scaffolds are suitable for tissue engineering applications.Interestingly,human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells cultured on the nanocomposite scaffolds exhibited higher cell viability,adhesion,and proliferation compared to cells cultured on tissue culture plates or neat P(VDF-TrFE) scaffolds.Nanocomposite scaffolds implanted into rats with or without hMSCs did not elicit immunological responses,as assessed by macroscopic analysis and histology.Importantly,nanocomposite scaffolds promoted angiogenesis,which was increased in scaffolds pre-seeded with hMSCs.Overall,our results highlight the potential of these novel P(VDF-TrFE)/ZnO nanocomposites for use in tissue engineering,due to their biocompatibility and ability to promote cell adhesion and angiogenesis. 相似文献