共查询到20条相似文献,搜索用时 0 毫秒
1.
Zheng Zheng Dongyu Li Zhiyang Liu Hui‐Qing Peng Herman H. Y. Sung Ryan T. K. Kwok Ian D. Williams Jacky W. Y. Lam Jun Qian Ben Zhong Tang 《Advanced materials (Deerfield Beach, Fla.)》2019,31(44)
Nonlinear optical microscopy has become a powerful tool in bioimaging research due to its unique capabilities of deep optical sectioning, high‐spatial‐resolution imaging, and 3D reconstruction of biological specimens. Developing organic fluorescent probes with strong nonlinear optical effects, in particular third‐harmonic generation (THG), is promising for exploiting nonlinear microscopic imaging for biomedical applications. Herein, a simple method for preparing organic nanocrystals based on an aggregation‐induced emission (AIE) luminogen (DCCN) with bright near‐infrared emission is successfully demonstrated. Aggregation‐induced nonlinear optical effects, including two‐photon fluorescence (2PF), three‐photon fluorescence (3PF), and THG, of DCCN are observed in nanoparticles, especially for crystalline nanoparticles. The nanocrystals of DCCN are successfully applied for 2PF microscopy at 1040 nm NIR‐II excitation and THG microscopy at 1560 nm NIR‐II excitation, respectively, to reconstruct the 3D vasculature of the mouse cerebral vasculature. Impressively, the THG microscopy provides much higher spatial resolution and brightness than the 2PF microscopy and can visualize small vessels with diameters of ≈2.7 µm at the deepest depth of 800 µm in a mouse brain. Thus, this is expected to inspire new insights into the development of advanced AIE materials with multiple nonlinearity, in particular THG, for multimodal nonlinear optical microscopy. 相似文献
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Wei Wu Yanqing Yang Yang Yang Yuming Yang Kaiyuan Zhang Li Guo Hongfei Ge Xiaowei Chen Jie Liu Hua Feng 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(20)
Design and synthesis of new fluorophores with emission in the second near‐infrared window (NIR‐II, 1000–1700 nm) have fueled the advancement of in vivo fluorescence imaging. Organic NIR‐II probes particularly attract tremendous attention due to excellent stability and biocompatibility, which facilitate clinical translation. However, reported organic NIR‐II fluorescent agents often suffer from low quantum yield and complicated design. In this study, the acceptor unit of a known NIR‐I aggregation‐induced emission (AIE) luminogen (AIEgen) is molecularly engineered by varying a single atom from sulfur to selenium, leading to redshifted absorption and emission spectra. After formulation of the newly prepared AIEgen, the resultant AIE nanoparticles (referred as L897 NPs) have an emission tail extending to 1200 nm with a high quantum yield of 5.8%. Based on the L897 NPs, noninvasive vessel imaging and lymphatic imaging are achieved with high signal‐to‐background ratio and deep penetration. Furthermore, the L897 NPs can be used as good contrast agents for tumor imaging and image‐guided surgery due to the high tumor/normal tissue ratio, which peaks at 9.0 ± 0.6. This work suggests a simple strategy for designing and manufacturing NIR‐II AIEgens and demonstrates the potential of NIR‐II AIEgens in vessel, lymphatic, and tumor imaging. 相似文献
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Junlong Geng Kai Li Wei Qin Lin Ma Gagik G. Gurzadyan Ben Zhong Tang Bin Liu 《Small (Weinheim an der Bergstrasse, Germany)》2013,9(11):2012-2019
A simple strategy is developed to prepare eccentrically or homogeneously loaded nanoparticles (NPs) using poly (DL‐lactide‐co‐glycolide) (PLGA) as the encapsulation matrix in the presence of different amounts of polyvinyl alcohol (PVA) as the emulsifier. Using 2,3‐bis(4‐(phenyl(4‐(1,2,2‐triphenylvinyl)‐phenyl)amino)‐phenyl)‐fumaronitrile (TPETPAFN), a fluorogen with aggregation‐induced emission (AIE) characteristics, as an example, the eccentrically loaded PLGA NPs show increased fluorescence quantum yields (QYs) as compared to the homogeneously loaded ones. Field emission transmission electron microscopy and fluorescence lifetime measurements reveal that the higher QY of the eccentrically loaded NPs is due to the more compact aggregation of AIE fluorogens that restricts intramolecular rotations of phenyl rings, which is able to more effectively block the non‐radiative decay pathways. The eccentrically loaded NPs show far red/near infrared emission with a high fluorescence QY of 34% in aqueous media. In addition, by using poly([lactide‐co‐glycolide]‐b‐folate [ethylene glycol]) (PLGA‐PEG‐folate) as the co‐encapsulation matrix, the obtained NPs are born with surface folic acid groups, which are successfully applied for targeted cellular imaging with good photostability and low cytotoxicity. Moreover, the developed strategy is also demonstrated for inorganic‐component eccentrically or homogeneously loaded PLGA NPs, which facilitates the synthesis of polymer NPs with controlled internal architectures. 相似文献
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Biocompatible Red Fluorescent Organic Nanoparticles with Tunable Size and Aggregation‐Induced Emission for Evaluation of Blood–Brain Barrier Damage 下载免费PDF全文
Xiaolei Cai Aishwarya Bandla Duo Mao Guangxue Feng Wei Qin Lun‐De Liao Nitish Thakor Ben Zhong Tang Bin Liu 《Advanced materials (Deerfield Beach, Fla.)》2016,28(39):8760-8765
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Precise Two‐Photon Photodynamic Therapy using an Efficient Photosensitizer with Aggregation‐Induced Emission Characteristics 下载免费PDF全文
Bobo Gu Wenbo Wu Gaixia Xu Guangxue Feng Feng Yin Peter Han Joo Chong Junle Qu Ken‐Tye Yong Bin Liu 《Advanced materials (Deerfield Beach, Fla.)》2017,29(28)
Two‐photon photodynamic therapy (PDT) is able to offer precise 3D manipulation of treatment volumes, providing a target level that is unattainable with current therapeutic techniques. The advancement of this technique is greatly hampered by the availability of photosensitizers with large two‐photon absorption (TPA) cross section, high reactive‐oxygen‐species (ROS) generation efficiency, and bright two‐photon fluorescence. Here, an effective photosensitizer with aggregation‐induced emission (AIE) characteristics is synthesized, characterized, and encapsulated into an amphiphilic block copolymer to form organic dots for two‐photon PDT applications. The AIE dots possess large TPA cross section, high ROS generation efficiency, and excellent photostability and biocompatibility, which overcomes the limitations of many conventional two‐photon photosensitizers. Outstanding therapeutic performance of the AIE dots in two‐photon PDT is demonstrated using in vitro cancer cell ablation and in vivo brain‐blood‐vessel closure as examples. This shows therapy precision up to 5 µm under two‐photon excitation. 相似文献
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Spatiotemporal Drug Release Visualized through a Drug Delivery System with Tunable Aggregation‐Induced Emission 下载免费PDF全文
Xiangdong Xue Yuanyuan Zhao Luru Dai Xu Zhang Xiaohong Hao Chunqiu Zhang Shuaidong Huo Juan Liu Chang Liu Anil Kumar Wei‐Qiang Chen Guozhang Zou Xing‐Jie Liang 《Advanced materials (Deerfield Beach, Fla.)》2014,26(5):712-717
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Real‐Time and High‐Resolution Bioimaging with Bright Aggregation‐Induced Emission Dots in Short‐Wave Infrared Region 下载免费PDF全文
Ji Qi Chaowei Sun Abudureheman Zebibula Hequn Zhang Ryan T. K. Kwok Xinyuan Zhao Wang Xi Jacky W. Y. Lam Jun Qian Ben Zhong Tang 《Advanced materials (Deerfield Beach, Fla.)》2018,30(12)
Fluorescence imaging in the spectral region beyond the conventional near‐infrared biological window (700–900 nm) can theoretically afford high resolution and deep tissue penetration. Although some efforts have been devoted to developing a short‐wave infrared (SWIR; 900–1700 nm) imaging modality in the past decade, long‐wavelength biomedical imaging is still suboptimal owing to the unsatisfactory materials properties of SWIR fluorophores. Taking advantage of organic dots based on an aggregation‐induced emission luminogen (AIEgen), herein microscopic vasculature imaging of brain and tumor is reported in living mice in the SWIR spectral region. The long‐wavelength emission of AIE dots with certain brightness facilitates resolving brain capillaries with high spatial resolution (≈3 µm) and deep penetration (800 µm). Owning to the deep penetration depth and real‐time imaging capability, in vivo SWIR microscopic angiography exhibits superior resolution in monitoring blood–brain barrier damage in mouse brain, and visualizing enhanced permeability and retention effect in tumor sites. Furthermore, the AIE dots show good biocompatibility, and no noticeable abnormalities, inflammations or lesions are observed in the main organs of the mice. This work will inspire new insights on development of advanced SWIR techniques for biomedical imaging. 相似文献
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A Luminogen with Aggregation‐Induced Emission Characteristics for Wash‐Free Bacterial Imaging,High‐Throughput Antibiotics Screening and Bacterial Susceptibility Evaluation 下载免费PDF全文
Engui Zhao Yilong Chen Sijie Chen Haiqin Deng Chen Gui Chris W. T. Leung Yuning Hong Jacky W. Y. Lam Ben Zhong Tang 《Advanced materials (Deerfield Beach, Fla.)》2015,27(33):4931-4937
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Activatable Fluorescent Nanoprobe with Aggregation‐Induced Emission Characteristics for Selective In Vivo Imaging of Elevated Peroxynitrite Generation 下载免费PDF全文
Zhegang Song Duo Mao Simon H. P. Sung Ryan T. K. Kwok Jacky W. Y. Lam Deling Kong Dan Ding Ben Zhong Tang 《Advanced materials (Deerfield Beach, Fla.)》2016,28(33):7249-7256
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Bright Aggregation‐Induced‐Emission Dots for Targeted Synergetic NIR‐II Fluorescence and NIR‐I Photoacoustic Imaging of Orthotopic Brain Tumors 下载免费PDF全文
Zonghai Sheng Bing Guo Dehong Hu Shidang Xu Wenbo Wu Weng Heng Liew Kui Yao Jingying Jiang Chengbo Liu Hairong Zheng Bin Liu 《Advanced materials (Deerfield Beach, Fla.)》2018,30(29)
Precise diagnostics are of significant importance to the optimal treatment outcomes of patients bearing brain tumors. NIR‐II fluorescence imaging holds great promise for brain‐tumor diagnostics with deep penetration and high sensitivity. This requires the development of organic NIR‐II fluorescent agents with high quantum yield (QY), which is difficult to achieve. Herein, the design and synthesis of a new NIR‐II fluorescent molecule with aggregation‐induced‐emission (AIE) characteristics is reported for orthotopic brain‐tumor imaging. Encapsulation of the molecule in a polymer matrix yields AIE dots showing a very high QY of 6.2% with a large absorptivity of 10.2 L g?1 cm?1 at 740 nm and an emission maximum near 1000 nm. Further decoration of the AIE dots with c‐RGD yields targeted AIE dots, which afford specific and selective tumor uptake, with a high signal/background ratio of 4.4 and resolution up to 38 µm. The large NIR absorptivity of the AIE dots facilitates NIR‐I photoacoustic imaging with intrinsically deeper penetration than NIR‐II fluorescence imaging and, more importantly, precise tumor‐depth detection through intact scalp and skull. This research demonstrates the promise of NIR‐II AIE molecules and their dots in dual NIR‐II fluorescence and NIR‐I photoacoustic imaging for precise brain cancer diagnostics. 相似文献
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A Highly Efficient and Photostable Photosensitizer with Near‐Infrared Aggregation‐Induced Emission for Image‐Guided Photodynamic Anticancer Therapy 下载免费PDF全文
Wenbo Wu Duo Mao Fang Hu Shidang Xu Chao Chen Chong‐Jing Zhang Xiamin Cheng Youyong Yuan Dan Ding Deling Kong Bin Liu 《Advanced materials (Deerfield Beach, Fla.)》2017,29(33)
Photodynamic therapy (PDT), which relies on photosensitizers (PS) and light to generate reactive oxygen species to kill cancer cells or bacteria, has attracted much attention in recent years. PSs with both bright emission and efficient singlet oxygen generation have also been used for image‐guided PDT. However, simultaneously achieving effective 1O2 generation, long wavelength absorption, and stable near‐infrared (NIR) emission with low dark toxicity in a single PS remains challenging. In addition, it is well known that when traditional PSs are made into nanoparticles, they encounter quenched fluorescence and reduced 1O2 production. In this contribution, these challenging issues have been successfully addressed through designing the first photostable photosensitizer with aggregation‐induced NIR emission and very effective 1O2 generation in aggregate state. The yielded nanoparticles show very effective 1O2 generation, bright NIR fluorescence centered at 820 nm, excellent photostability, good biocompatibility, and negligible dark in vivo toxicity. Both in vitro and in vivo experiments prove that the nanoparticles are excellent candidates for image‐guided photodynamic anticancer therapy. 相似文献
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Xing‐Huo Wang Nan Song Wei Hou Chun‐Yu Wang Yan Wang Jun Tang Ying‐Wei Yang 《Advanced materials (Deerfield Beach, Fla.)》2019,31(37)
Linear copolymer hosts bearing a number of pillar[5]arene dangling side chains are synthesized for the facile construction of highly emissive supramolecular polymer networks (SPNs) upon noncovalently cross‐linking with a series of tetraphenyethylene (TPE)‐based tetratopic guests terminated with different functional groups through supramolecular host–guest interactions. An extremely high fluorescence quantum yield (98.22%) of the SPNs materials is obtained in tetrahydrofuran (THF) by fine‐tuning the parameters, and meanwhile supramolecular light‐harvesting systems based on spherical supramolecular nanoparticles are constructed by interweaving 9,10‐distyrylanthracene (DSA) and TPE‐based guest molecules of aggregation‐induced emission (AIE) with the copolymer hosts in the mixed solvent of THF/H2O. The present study not only illustrates the restriction of the intramolecular rotations (RIR)‐ruled emission enhancement mechanism regulated particularly by macrocyclic arene‐containing copolymer hosts, but also suggests a new self‐assembly approach to construct high‐performance light‐harvesting materials. 相似文献
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Highly Efficient Photosensitizers with Far‐Red/Near‐Infrared Aggregation‐Induced Emission for In Vitro and In Vivo Cancer Theranostics 下载免费PDF全文
Dong Wang Michelle M. S. Lee Guogang Shan Ryan T. K. Kwok Jacky W. Y. Lam Huifang Su Yuchen Cai Ben Zhong Tang 《Advanced materials (Deerfield Beach, Fla.)》2018,30(39)
Fluorescence‐imaging‐guided photodynamic therapy has emerged as a promising protocol for cancer theranostics. However, facile preparation of such a theranostic material for simultaneously achieving bright emission with long wavelength, high‐performance reactive oxygen species (ROS) generation, and good targeting‐specificity of cancer cells, is highly desirable but remains challenging. In this study, a novel type of far‐red/near‐infrared‐emissive fluorescent molecules with aggregation‐induced emission (AIE) characteristics is synthesized through a few steps reaction. These AIE luminogens (AIEgens) possess simple structures, excellent photostabilities, large Stokes shifts, bright emission, and good biocompatibilities. Meanwhile, their ROS generation is extremely efficient with up to 90.7% of ROS quantum yield, which is far superior to that of some popularly used photosensitizers. Importantly, these AIEgens are able to selectively target and ablate cancer cells over normal cells without the aid of any extra targeting ligands. Rather than using laser light, one of the presented AIEgens (MeTTPy) shows a remarkable tumor‐targeting photodynamic therapeutic effect by using an ultralow‐power lamp light (18 mW cm?2). This study thus not only extends the applications scope of AIEgens, but also offers useful insights into designing a new generation of cancer theranostics. 相似文献
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Tao Wang Xiaoge Su Xuepeng Zhang Xiancheng Nie Linkun Huang Xingyuan Zhang Xiang Sun Yi Luo Guoqing Zhang 《Advanced materials (Deerfield Beach, Fla.)》2019,31(51)
Aggregation‐induced emission (AIE) is a beneficial strategy for generating highly effective solid‐state molecular luminescence without suffering losses in quantum yield. However, the majority of reported AIE‐active molecules exhibit only strong fluorescence, which is not ideal for electrical excitation in organic light‐emitting diodes (OLEDs). By introducing various substituent groups onto the biscarbazole compound, a series of molecular materials with aggregation‐induced phosphorescence (AIP) is designed, which exhibits two distinctly different phosphorescence bands and an absolute solid‐state room‐temperature phosphorescence quantum yield up to 64%. Taking advantage of the AIE feature, the AIP molecules are fabricated into OLEDs as a homogeneous light‐emitting layer, which allows for relatively small efficiency roll‐off and shows an external electroluminescence quantum yield of up to 5.8%, more than the theoretical limit for purely fluorescent OLED devices. The design showcases a promising strategy for the production of cost‐effective and highly efficient OLED technology. 相似文献
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Junlong Geng Kai Li Dan Ding Xinhai Zhang Wei Qin Jianzhao Liu Ben Zhong Tang Bin Liu 《Small (Weinheim an der Bergstrasse, Germany)》2012,8(23):3655-3663
Folate functionalized nanoparticles (NPs) that contain fluorogens with aggregation‐induced emission (AIE) characteristics are fabricated to show bright far‐red/near‐infrared fluorescence, a large two‐photon absorption cross section and low cytotoxicity, which are internalized into MCF‐7 cancer cells mainly through caveolae‐mediated endocytosis. One‐photon excited in vivo fluorescence imaging illustrates that these AIE NPs can accumulate in a tumor and two‐photon excited ex vivo tumor tissue imaging reveals that they can be easily detected in the tumor mass at a depth of 400 μm. These studies indicate that AIE NPs are promising alternatives to conventional TPA probes for biological imaging. 相似文献
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Mitochondrion‐Anchoring Photosensitizer with Aggregation‐Induced Emission Characteristics Synergistically Boosts the Radiosensitivity of Cancer Cells to Ionizing Radiation 下载免费PDF全文
Chris Y. Y. Yu Huae Xu Shenglu Ji Ryan T. K. Kwok Jacky W. Y. Lam Xiaolin Li Sunil Krishnan Dan Ding Ben Zhong Tang 《Advanced materials (Deerfield Beach, Fla.)》2017,29(15)
The first mitochondrion‐anchoring photosensitizer that specifically generates singlet oxygen (1O2) in mitochondria under white light irradiation that can serve as a highly effective radiosensitizer is reported here, significantly sensitizing cancer cells to ionizing radiation. An aggregation‐induced emission luminogen (AIEgen), namely DPA‐SCP, is rationally designed with α‐cyanostilbene as a simple building block to reveal AIE, diphenylamino (DPA) group as a strong electron donating group to benefit red emission and efficient light‐controlled 1O2 generation, as well as a pyridinium salt as the targeting moiety to ensure specific mitochondrial localization. The AIE signature endows DPA‐SCP with the capacity to visualize mitochondria in a fluorescence turn‐on mode. It is found that under optimized experimental condition, DPA‐SCP with white light does not lead to apoptosis/death of cancer cells, whereas provides an elevated 1O2 environment in the mitochondria. More importantly, increasing intracellular level of 1O2 originated from mitochondria is demonstrated to be a generic method to enhance the radiosensitivity of cancer cells with a supra‐additive synergistic effect of “0 + 1 > 1.” Noteworthy is that “DPA‐SCP + white light” achieves a high SER10 value of 1.62, which is much larger than that of the most popularly used radiosensitizers, gold nanoparticles (1.19), and paclitaxel (1.32). 相似文献
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Photoacoustic Imaging: Bright Aggregation‐Induced‐Emission Dots for Targeted Synergetic NIR‐II Fluorescence and NIR‐I Photoacoustic Imaging of Orthotopic Brain Tumors (Adv. Mater. 29/2018) 下载免费PDF全文
Zonghai Sheng Bing Guo Dehong Hu Shidang Xu Wenbo Wu Weng Heng Liew Kui Yao Jingying Jiang Chengbo Liu Hairong Zheng Bin Liu 《Advanced materials (Deerfield Beach, Fla.)》2018,30(29)