Efficacy of multi-mode adhesive systems on dentin wettability and microtensile bond strength of resin composite

Purpose: To evaluate the wetting ability and the microtensile bond strength of adhesive systems in various depths of dentin. Materials and Method: 48 extracted human molars cut in half in buccolingual direction. Buccal and lingual surfaces were used to obtain deep (n = 48) and superficial (n = 48) dentin. Groups were divided into 4 subgroups: Self-etch (CSE), etch&rinse (SB), multi-mode self-etch (SAU) and multimode etch&rinse (EAU) adhesive systems. 3 consecutive contact-angle measurements were obtained: T0- 3 μl drop of distilled water on dentin; T1-Droplet of the adhesive; T2- Distilled water after polymerization of the adhesive. After composite build-ups, microtensile measurements were performed. Contact angle data were analysed with analysis of variance for repeated measures. Bond strength data were analyzed by repeated measures analysis of variance, comparisons were made according to the logarithmic values (p < 0.05). Results: The difference between groups was not significant regardless of dentin depth for all measurements (p < 0.05). All groups except CSE enhanced the wetting ability of the adhesive but reduced the wetting ability of distilled water after application of the adhesive (p < 0.05). Regarding adhesive systems, the groups showed no significant difference between bond strengths to various depths of dentin except SAU (p > 0.05); in SAU, bond strength to deep dentine were significantly higher than superficial dentin (p < 0.05). Regarding adhesives’ bond strength, CSE showed significantly greater values than the other groups (p < 0.05). Conclusion: The cavity depth does not affect the bonding ability for all adhesive systems; self-etch adhesive systems might be a better choice since different adhesives may influence the wetting ability and microtensile bond strength of the dentin substrates.     

Effect of zinc chloride added to self-etching primer on bond strength to caries-affected dentin and chemical-physical-mechanical properties of adhesives

The aim of this in vitro study was to evaluate the effect over time of zinc chloride (ZnCl₂) incorporated into the primer of a two-step self-etching adhesive system (Clearfil SE Bond, Kuraray - SE) on long-term microtensile bond strength (μTBS) to caries-affected dentin (CAD), and on flexural strength (FS) and conversion degree (CD) of the adhesive. First, the CD of SE with and without 2% (wt) ZnCl₂ solution was evaluated by Fourier transform infrared spectroscopy (FTIR). Then, beam-shaped samples (7 mm × 2 mm x 1 mm) were prepared with the SE primer containing the ZnCl₂ solution, to perform flexural strength (FS) tests. For μTBS testing purposes, CAD surfaces were randomly divided into two groups, according to the presence of ZnCl₂ powder (2 wt%) incorporated into the adhesive system (ZnCl₂), or its absence (NT). An additional group consisting of dentin pretreated with a 2% chlorhexidine digluconate (CHX) solution was proposed as a positive control (n = 10). A two-step self-etching system (SE) was applied following the manufacturer's instructions, and restorations of composite resin were built up on the dentin. After 24 h, the resin-dentin blocks were sectioned into specimens that were submitted to μTBS testing immediately following, or after 12 months of water storage (WS). Both μTBS and FS tests were performed using a universal testing machine (0.5 mm/min). FS and CD data were submitted to the Student t-test, and μTBS data were submitted to two-way ANOVA and Tukey's test (α = 0.05). ZnCl₂ incorporation had no statistically significant influence on flexural strength (p = 0.88) or conversion degree (p = 566). Regarding μTBS, no significant effect of the double interaction of “dentin treatment” versus “WS period” (p = 0.546) was observed. The bond strength was not affected by the WS period (p = 0.805). The highest mean bond strength was observed for the NT group, which did not differ from the ZnCl₂ group. The lowest mean bond strength was observed for the CHX group, which differed statistically from the NT group (p = 0.053). It was concluded that incorporation of ZnCl₂ into the SE self-etching primer did not interfere in the bond strength of caries-affected dentin, in regard to failure mode, flexural strength or CD, and had no other beneficial effects.     

The effect of different cementing strategies and adhesive interface aging on microtensile bond strength (μTBS) of lithium disilicate ceramics to dentin

This study evaluated the effect of different cementing strategies and adhesive interface aging on microtensile bond strength (μTBS) of lithium disilicate ceramic (IPS e.max CAD) to dentin. Forty coronal dentin fragments were randomly assigned to four groups according to the cementing strategy used to bond lithium disilicate ceramic to coronal dentin surface (n = 10): U200 (self-adhesive resin cement (RC) RelyX U200®/3 M ESPE), SBU (single-step self-etching adhesive system (AS) Single Bond Universal®/3 M ESPE + RelyX ARC®/3 M ESPE RC), AdperSB (two-step etch-and-rinse AS Single Bond 2®/3 M + RelyX ARC®/3 M ESPE RC) and Scotchbond (three-step etch-and-rinse AS Scotchbond Multi-Purpose®/3 M + RelyX ARC®/3 M RC). After 48 h, the ceramic-tooth blocks were sectioned perpendicular to the adhesive interface in the form of sticks and randomly subdivided into two groups according to when they were to be submitted to μTBS testing: immediately or 6 months after storage in water. Some sticks were kept for analysis of the adhesive interface by scanning electron microscopy (SEM). The μTBS test was performed in a universal testing machine (0.5 mm/min). The data (MPa) were analyzed using split-plot ANOVA and Tukey’s test (α = 0.05). Water storage decreased μTBS in all cementing strategies. The μTBS was greatest in the Scotchbond group and lowest in the U200 group, at both storage times. No signs of interface degradation were detected under SEM after water storage. In conclusion, water storage decreased bond strength, regardless of the adhesive cementation strategy, and that the three-step adhesive system/dual-cure resin cement ultimately performed better in terms of bond strength.     

Influence of storage time on bond strength of self-etching adhesive systems to artificially demineralized dentin after a papain gel chemical–mechanical agent application

ObjectivesThe aim of this study was to evaluate the influence of storage time on the bond strength of self-etching adhesive systems to artificially demineralized dentin submitted to application of a papain-based chemical mechanical agent for carious tissue removal.Materials and MethodsTwenty-four blocks of human coronal dentin were randomly divided into 2 groups: (1) restored with a two-step self-etching adhesive system (Clearfil SE Bond); (2) restored with a one-step self-etching adhesive system (One-Up Bond F Plus). After artificial caries induction, the specimens were treated with papain-based gel, received an application of the adhesive system according to the respective group, and blocks of microhybrid resin composite measuring 5.0 mm high and 5.0 mm wide were fabricated incrementally on the tooth, which would later be cut to obtain sticks with a bond area of around 1 mm², for use in the microtensile tests. After this, half of the sticks obtained from each tooth were submitted to the microtensile test 24 h later, while the other half were stored in water, in an oven at 37° C for a period of 180 d. The specimens were submitted to the microtensile test in a Universal Test Machine at a speed of 0.5 mm/min. The surfaces of the fractured test specimens were examined visually under a stereoscopic loupe in order to classify the fracture type. After exploratory analysis of the data, the two-way Analysis of Variance (ANOVA) and the Tukey test were applied. The data on the fracture types were analyzed by the Exact Fisher test.ResultsNo statistically relevant differences were verified among the means of the microtensile bond strength values of the adhesive systems evaluated in the different storage times. Mixed and adhesive fractures types were observed for both groups.ConclusionStorage time did not influence the bond strength of the two-step or one-step self-etching adhesive systems to artificially demineralized dentin submitted to application of a papain-based chemical mechanical agent for carious tissue removal. Both adhesive systems provided similar bond strength at different storage times.     

Microtensile versus microshear bond strength between dental adhesives and the dentin substrate

This study evaluated the performance of different bonding tests on the bond strength of three adhesive systems to dentin. Forty-eight bovine incisors were used in the study, which were allocated in two groups according to the bond strength test performed: microtensile (µTBS) or microshear (µSBS). Also, each group was divided in three subgroups according to the adhesive system applied: Single Bond (SB), Prime & Bond (PB) and XP Bond (XPB) (n=8). The teeth were prepared according to each bond strength test protocol, and then the specimens were tested in an universal testing machine (EMIC). The data were submitted to two-way ANOVA (bond strength test and adhesive system type factors) and Tukey's post hoc test (α=0.05). The µTBS results were higher for all the adhesives when compared to the µSBS ones. Within the µTBS results, SB showed higher bond strength than PB and XPB, which showed similar adhesive performance among each other; within the µSBS results, all the adhesives presented similar bond strength values to dentin. The µTBS test detected differences between the adhesive systems evaluated; in contrast, the µSBS test was less discriminating in evaluating the adhesive performance of the materials tested.     

Influence of pH cycling and mechanical loading on the resin-dentin microtensile bond strength

The durability of adhesive interfaces is still a problem in adhesive restorations in dentistry. Laboratorial ageing methods have been proposed to predict the performance of materials, but they still require standardization. The objective of this study was to evaluate the resin-dentin microtensile bond strength of composite restorations subjected to pH cycling and different levels of mechanical loading. Resin blocks were built over a flat superficial dentin of bovine incisors (n = 40), using a three-step adhesive system. Half of the specimens were submitted to 10 cycles of pH cycling, followed by mechanical loading (0, 500,000; 1,000,000; 2,000,000 cycles – 98 N, 4 Hz). The other half was directly subjected to mechanical loading. After ageing, all groups were subjected to a microtensile bond test. Data were analyzed using two-way ANOVA and Tukey’s test (α = 0.05). The frequency of the early de-bonding was compared using the Chi-square test (α = 0.05). The statistical analysis revealed that the cross-product interaction ‘pH cycling’ vs. ‘number of mechanical loading cycles’ (p = 0.72) as well as the main factor ‘pH cycling’ (p = 0.49) was not statistically significant, and they were not able to reduce the resin–dentin bond strength values. Regarding the number of mechanical loading cycles, the groups subjected to 2,000,000 cycles showed lower bond strength values than the others (p = 0.003). The frequency of debonded specimens increased with mechanical loading. Therefore, it can be concluded that in this restorative model, at least 2,000,000 mechanical cycles are need to decrease the microtensile strength and simulate the ageing of the interface.     

Post-treatment with high-power lasers to improve bond strength of adhesive systems to bleached dentin

To assess the effect of Er:YAG and diode lasers on the shear bond strength (SBS) of adhesive systems to bovine dentin submitted to bleaching with a high concentration agent. One hundred and twenty bovine dentin fragments were used. Fragments were distributed into 12 groups (n = 10) considering the bleaching (present or not), surface post-treatment (untreated, Er:YAG laser or diode laser) and adhesive system (total-etching or self-etching). Specimens received two applications of 38% hydrogen peroxide. Er:YAG laser (2940 nm, 200 mJ, 4 Hz) and diode laser (980 nm, 1.5 W) were applied for 15 s on bleached dentin surface. Restoration was performed with resin using split matrix. Specimens were submitted to SBS test and data (MPa) were analyzed by ANOVA and Tukey’s test (α = 0.05). SBS of bleached specimens decreased in comparison with non-bleached (p < 0.05). The highest values were obtained for the post-treatment with Er:YAG laser (p < 0.05). Total-etching adhesive was superior to self-etching system (p < 0.05). The irradiation of bleached dentin with Er:YAG laser followed by the application of the total-etching adhesive had similar SBS to unbleached dentin with no post-treatment (control) (p > 0.05). Er:YAG laser post-treatment followed by the total-etching adhesive system improve the bond strength of restorative material to bleached dentin.     

Influence of chlorhexidine in cavities prepared with ultrasonic or diamond tips on microtensile bond strength

This study evaluated the effect of chlorhexidine (CLX) in cavities prepared with either ultrasound-mounted CVDentUS diamond tips or conventional diamond burs on dentin bond strength after 24 h and 180 days. Forty-eight dentin surfaces from sound third molars were flattened and divided into four groups (n = 12), according to the type of cavity preparation (CVDentUS ultrasound tip or conventional diamond) and with or without 2% CLX (Consepsis) treatment. After application of the adhesive system (Clearfil SE Bond), microhybrid composite resin blocks (Charisma) were made on the dentin surface in increments. After 24 h, the specimens were sectioned into stick-shaped samples with an adhesive interface of approximately 1 mm². From each tooth, half of the sticks were evaluated after 24 h of storage in distilled water and the other half after 180 days, using a universal testing machine. Three-way analysis of variance showed no significant triple interactions (p = 0.722) or double interactions between factors. Higher bond strength values were observed with the use of ultrasonic tips (p = 0.019), irrespective of whether or not CLX was applied in either time period. No difference in bond strength values was observed in terms of CLX application (p = 0.581). No significant difference in bond strength values was shown after storage for 24 h or 180 days (p = 0.302). In conclusion, the ultrasonic tips promoted greater bond strength to dentin, irrespective of whether or not CLX was applied, and storage time.     

Effect of self-etching dual-curing universal adhesive system application on bond strength of dentin resinous liners dentin

Purpose: The aim of this study was to evaluate the influence of previous application of an adhesive system on bond strength of resinous liner materials to dentin. Methods: Ninety bovine incisors crowns had a 6 × 6 mm area of dentin exposed, with minimum of 2 mm thickness. They were embedded in acrylic resin, and the dentin was polished with P600 SiC sandpaper for 30 s to standardize the smear layer. The specimens were divided into 6 groups (n = 15) according to the application or not of a self-etching system (Futurabond U – Voco) and the type of resinous liner used: A+Ionoseal (adhesive and Ionoseal – Voco); Ionoseal (Ionoseal only); A+Vitrebond (adhesive and Vitrebond – 3M/ESPE); Vitrebond (Vitrebond only); A+Ionosit (adhesive and Ionosit – DMG) and Ionosit (Ionosit only). Adhesives were used following manufacturer’s instructions, and the liner materials were applied inside a 2-mm-depth matrix and light-cured for 20 s. The bond strength was measured by microtensile test, using a universal testing machine with a cross-speed of 1 mm/min. Data were analyzed using one-way ANOVA and Tukey’s test (p < 0.05). Results: The adhesive system application increased bond strength of all liners tested. Ionoseal presented the highest bond strength when the adhesive system was used and exhibited similar performance to Vitrebond without adhesive. Ionosit without adhesive showed the smallest bond strength compared with the other liners tested. Conclusion: The application of an adhesive system prior to the use of the resinous liners improved the bond strength to dentin and should be preconized.     

The influence of blood and/or hemostatic agent contamination on Micro-TBS to dentin

Aim: The purpose of this study was to evaluate the effects of blood contamination and hemostatic agent Ankaferd Blood Stopper (ABS: Ankaferd Drug Inc, Istanbul, Turkey) on the microtensile bond strength of a self-etching adhesive. Material and methods: Flat dentin surfaces were created from 40-M teeth and randomly divided to four groups according to contamination and adhesive procedure. The specimens of Group 1 are contaminated with blood; ABS was applied to the specimens of Group 2 after blood contamination and applied to the specimens of Group 3 without blood contamination. Group 4 is control group and self-etching adhesive was applied to all specimens. Teeth were restored with a nanohybrid composite. The specimens were sectioned to the beams and microtensile testing was carried out and the data were statistically analyzed with analysis of variance test. Tukey’s honestly significant difference post hoc test was also performed for multiple comparisons to compare subgroups. Results: Group 4 had the highest strength value, followed by Group 3, while Group 1, which contacts only with blood, had the lowest strength value. Conclusion: ABS has a negative effect on the bond strength of one-step self-etching adhesive system.     

Incorporation of EGCG into an etch-and-rinse adhesive system: mechanical properties and bond strength to caries affected dentin

This study evaluated how a matrix metalloproteinase (MMP) inhibitor (EGCG) incorporated into an etch-and-rinse adhesive system (AS) affects the long-term microtensile bond strength (MTBS) to caries affected dentin (CAD) and flexural strength (FS) of AS. Forty CAD surfaces received acid conditioning and were randomly divided into four groups (n?=?10): EGCG-PRE – dentin treatment with 200?μg/mL EGCG solution; EGCG-INC – 200?μg/mL EGCG solution incorporated into SA; CHX-PRE – dentin treatment with 2% digluconate solution; NT – no treatment. The AS (Adper Single Bond 2, 3M ESPE) was applied and resin composite blocks were built on the dentin. Beam-shaped specimens (0.8?mm²) were obtained submitted to MTBS testing following 24?h, 6 months or 1 year of water storage (WS). Adhesive interface was analyzed by SEM. FS testing was performed by preparing specimens with the adhesive system, with or without the EGCG solution (200?μg/mL) (n?=?10). Tests were carried out in a universal machine (0.5?mm/min). MTBS data were submitted to two-way ANOVA and Tukey’s test, whereas FS data, to the Student’s t-test (α?=?0.05). MTBS values were not affected by EGCG application, either incorporated or used as a dentin treatment agent, regardless of the WS period; however, CHX negatively affected MTBS to CAD (p?<?0.001). WS significantly reduced MTBS values (p?<?0.001) from the 6-months time point. FS was not affected by EGCG incorporation into the SA (p?=?0.2527). EGCG incorporation into AS was unable to maintain bond stability to CAD over time, whereas it did not compromise adhesive’s property.     

Effect of caries removal techniques on bond strength to caries affected dentin on gingival wall: AFM observation of dentinal surface

Objective: The purpose of this study was to evaluate the effect of different caries removal techniques on the bond strength of two adhesive systems. Materials and Methods: Seventy human molar teeth that had proximal carious lesions were randomly divided into four groups according to the technique used to remove the caries: a conventional steel bur, an Er:YAG laser, a chemomechanical Carisolv^® gel, and air polishing. The groups were then divided into two subgroups according to the adhesives used: Clearfil S³ (Kuraray Co., Ltd, Tokyo, Japan) and Adper SE Plus (3 M ESPE, St Paul, MN, USA). The enamel and superficial dentin of the crown was flattened and caries were removed using different methods. Teeth were restored with composite and three 1 mm² stick-shaped microtensile specimens were prepared from each tooth. For each removal technique, the surface roughness of one dentin sample was analyzed using atomic force microscopy (AFM). The microtensile bond strength data were then analyzed using a two-way ANOVA, Kruskal–Wallis and Mann–Whitney U tests, and multiple comparison tests (p = 0.05). Results: The two-way ANOVA showed that the effects of the cavity preparation techniques and adhesive systems and their interaction were statistically significant (p < 0.05). The μTBS values were highest when bonding with Adper SE Plus to cavities prepared with the steel bur (37.20 ± 11.65 MPa) and lowest when bonding with Clearfil S³ Bond to cavities prepared with the Er:YAG laser (16.74 ± 6.95 MPa). Conclusion: The caries removal techniques affected the bond strength values of the self-etch adhesive systems.     

Effect of epigallocatechin gallate,green tea extract and chlorhexidine application on long-term bond strength of self-etch adhesive to dentin

This study evaluated the effect of dentin pretreatment with metalloproteinase inhibitors on long-term microtensile bond strength (μTBS) of self-etch adhesive (SEA) to dentin. Thirty-two dentin fragments received pretreatments (n=8): C: control (not treated); GT: 2% green tea extract; CLX: 2% chlorhexidine; EGCG: 2% epigallocatechin gallate. SEAs (Clearfil SE Bond) and resin composite were applied to dentin surface. After 24 h, resin-blocks were cut into sticks submitted to μTBS testing immediately or after 6 months of storage in water. Two-way ANOVA and Tukey test (α=5%) indicated that CLX group showed μTBS values statistically superior to the other groups at immediate testing, whereas those of EGCG group were the lowest (p=0.018). After 6 months, a significant increase in μTBS values was observed for all groups, except for CLX (p=0.018). In conclusion, μTBS increased in dentin pretreatment with EGCG and green tea groups, and in the non-treated group.     

Effect of Different Application Techniques of All-in-One Adhesives on Microtensile Bond Strength to Sound and Caries-Affected Dentin

The aim of this study was to evaluate the microtensile bond strength of two all-in-one self-etch adhesives applied to sound and caries-affected dentin with four different application techniques. Forty extracted third molars with occlusal caries were randomly divided into four groups for G-Aenial Bond and S3 Bond: (1) according to manufacturer's instructions; (2) with acid etching before applying adhesives; (3) doubling adhesive application time; and (4) doubling adhesive coating. Teeth were sectioned to obtain 1-mm- ± 0.2-mm-thick dentin sticks and subjected to a tensile force. For G-Aenial Bond, doubling the time and application of two consecutive coats produced significantly higher strength than that obtained by following manufacturers' instructions and acid etching application to sound dentin. Prior acid etching and application of two consecutive coats to caries-affected dentin generated significantly higher bond strength than that using other techniques. For S3 Bond, there was no significant difference between application techniques in caries-affected dentin. For sound dentin, double-time application of S3 Bond produced significantly higher strength than application according to the manufacturers' instructions. Compared to the application according to manufacturer's instructions, acid etching before applying adhesives, doubling application time, and doubling the coating had a positive effect on bond strength to caries-affected dentin for all-in-one adhesives.     

Effects of cavity disinfectants on bond strength of an etch-and-rinse adhesive to water- or ethanol-saturated sound and caries-affected dentin

Objective: To evaluate the effect of cavity disinfectants on the immediate microtensile bond strength (μTBS) of an etch-and-rinse adhesive to water- and ethanol-saturated sound and caries-affected dentin (CAD). Material and Methods: Thirty-six human molars were sectioned to expose 1/3 of the mid-coronal dentin surface. Sound (n = 18) and CAD (n = 18) specimens were divided into six groups each (n = 3): one positive control (sound), one negative control (CAD), and five experimental groups each. In the control group, dentin surfaces were bonded using an etch-and-rinse adhesive with a traditional water-wet bonding technique. In the experimental groups, ozone was applied before etching and chlorhexidine after etching. In the ethanol-wet bonding groups, acid-etched dentin surfaces were treated with ethanol. Following adhesive application and composite buildups, bonded specimens were sectioned to form sticks. Failure modes were analyzed using a stereomicroscope. Results: The water-wet bonded sound control group yielded the highest μTBS among all groups (p < 0.001). The lowest μTBS values were observed in the ozone groups (p < 0.05). The ethanol-wet bonded CAD group exhibited a higher μTBS than the water-wet bonded negative controls. Although compared to the positive control, chlorhexidine decreased the μTBS (p < 0.05), an increase with no significant difference was observed in the negative control (p > 0.05). Conclusions: The μTBS values of CAD were lower than those of sound dentin. Ethanol-wet bonding improved the μTBS of CAD. Ozone application reduced the μTBS in both sound and CAD; chlorhexidine improved the immediate μTBS after etching in CAD.     

Which is effective on bond strength of resin-based sealers: incorporation of powdered dentin to primer or adhesive?

The aim of this study was to investigate the effect of adding powdered dentin to primer or adhesive in a self-etch system on the shear bond strength of three resin-based sealers. Seventy-two premolars were sectioned buccolingually, and 144 root halves were divided into three groups according to the sealer used: epoxy resin-based sealer (AH Plus), methacrylate resin-based (RealSeal, Hybrid Root SEAL) n = 48. The surfaces were irrigated with 5% NaOCl, 17% EDTA, distilled water for 5 min. Four subgroups were created (n = 12): control group; Clearfil Liner Bond 2 V treated group; powdered dentin added to the primer of Clearfil Liner Bond 2 V (40 wt.%); and powdered dentin added to the adhesive of Clearfil Liner Bond 2 V (20 wt.%). Dentin powder was prepared. Three mm high buildups with a constant surface area of 3.45 mm² were created using the sealers and allowed to set (37 ºC, 100% humid, 72 h). The samples were tested to failure for shear bond strength (1 mm/min). The data were calculated (MPa) and analyzed using two-way ANOVA, one-way ANOVA, and Tukey HSD tests. Adhesive use decreased the bonding performance of AH Plus (p = 0.00). Mean bond strength of the other sealers was found similar to control. Primer or adhesive resin with powdered dentin did not increase the adhesive performance of the self-etch system used. The shear bond strength of RealSeal was significantly increased when powdered dentin was added to primer or adhesive (p = 0.00). The effect of adding powdered-dentine to primer or adhesive in a self-etch system on the shear bond strength was sealer-dependent .     

Iodonium salt improves the dentin bonding performance in an experimental dental adhesive resin

The aim of this study was to evaluate the influence of an iodonium salt on the immediate and long-term microtensile bond strength to dentin of an experimental dental two-step, self-etching adhesive system. A model dental adhesive resin was formulated using bisphenol A glicidyl dimethacrylate (Bis-GMA), triethylene glicol dimethacrylate (TEGDMA), and hydroxyethyl methacrylate (HEMA), which was polymerized using four combinations of photo-initiators and coinitiators. A group using only camphorquinone (CQ), two binary systems (CQ and ethyl 4-dimethylamine benzoate (CQ+EDAB), CQ and diphenyliodonium hexafluorphosphate (CQ+DPIHFP)) and a ternary system (CQ+EDAB+DPIHFP) was investigated. Clearfil SE Bond (CSEB) was used as a commercial reference. Bond strength to bovine dentin was assessed through microtensile bond tests measured in MPa in a universal testing machine. The beam specimens were stored in distilled water at 37 °C for 24 h and 1 year. Two-way ANOVA and Student–Newman–Keuls' post-hoc tests were used to compare means of groups (α=5%) and failure modes classified under magnification. After 24 h, the ternary system showed a bond strength similar to that of the binary system CQ+EDAB and the commercial material. After 1 year of storage, the μTBS values of the group CQ+EDAB+DPIHFP showed a bond strength significantly higher (p<0.05) than those of the CQ, CQ+DPIHFP, and CQ+EDAB groups and no difference from that of the CSEB group. The ternary photo-initiator system with iodonium salt maintained increased bond strength to dentin of model self-etching adhesive systems after 1 year of aging.     

Bond Strength and Interfacial Ultramorphology of Current Adhesive Systems

This study evaluated the bond strength and ultramorphology of the resin-dentin interfaces produced by current dental adhesive systems. Nine dentin bonding agents were investigated. Restored teeth were vertically, serially sectioned to obtain bonded slices for interfacial TEM analysis or to produce bonded beams for the microtensile bond strength test. The one-step self-etching adhesives (Futurabond® NR and Hybrid Bond®) showed lower bond strength values than the three-step etch-&-rinse adhesive system All-Bond 3. Most bonding agents presented statistically similar mean bond strength values, which ranged from 41.3 ± 17.9 to 35.0 ± 5.3 MPa. The thickness of the hybrid layer varied according to the type of adhesive system used. While the etch and rinse adhesives with alcohol as organic solvent showed bond strength means higher than 40 MPa, the self-etching systems showed bond strength lower than 40 MPa. Resin-dentin interdifusion zone and resin tags were noted in all bonded interfaces.     

EFFECTS OF SURFACE HYDRATION AND APPLICATION METHOD ON THE BOND STRENGTH OF SELF-ETCHING ADHESIVES TO DENTIN

The effect of application methods and dentin hydration on the bond strength of three self-etching adhesives (SEA) were evaluated; 195 extracted bovine incisors were used. The buccal surface was ground in order to expose the dentin, which remained 2-mm minimum thickness, measured by a thickness meter through an opening on the lingual surface. Adper Single Bond 2? was used for the control group. The SEA were applied following two modes of application: passive or active and two hydration states of the dentin surface—dry and wet. After light-curing, composite buildups were made using Grandio? composite. The specimens were sectioned and tested with a microtensile bond strength test. The application method and the hydration state resulted in statistical differences (p = 0.000) making the values of active application for μTBS to dentin higher than passive application. The wet surfaces showed higher μTBS to dentin ratios than dry surfaces. There were no statistical differences in μTBS among the SEA tested but there were differences regarding to control group.     

Bonding ability of self-adhesive resin-cements after dentin biomodification with hyaluronic acid

Purpose: The present study evaluated the influence of the hyaluronic acid (HA) on the bonding ability of self-adhesive resin cements to dentin regarding the bond strength. Eighty bovine incisors were ground flat to obtain a 2-mm thick slices which received conical preparations. The specimens were randomly distributed into 4 groups (n = 15) according to the dentin pretreatment (1 – control: untreated dentin; 2 – application of HA) and the evaluation time (1 – control: immediate evaluation; 2 – hydrolytic degradation: 6 months of storage in water at 37 °C). Preparations received the application of a self-adhesive resin cement (RelyX U200 or MaxCem Elite). Push-out bond strength test was conducted (0.5 mm/min). The bond strength data was submitted to two-way ANOVA/Tukey’s test (α = 0.05). For U200, no significance was observed when comparing the immediate (24 h) and 6 months means for the control groups (unexposed specimens). Previous application of HA to dentin significantly reduced the bond strength of U200 to dentin in both evaluation times (p < 0.05). HA had no significant influence on the push-out bond strength means for the cement MAX in both evaluation times (p > 0.05). The type 1 failure mode (adhesive mode) occurred in 100% of the specimens, irrespective of the dentin treatment or evaluation times. Pretreatment of dentin with HA produces a material-dependent influence on the push-out bond strength. The bonding ability of RelyX U200 is negatively influenced by the pretreatment of dentin with HA, whereas the biomodification of dentin with this bioactive agent causes no impact for the cement MaxCem Elite.