Advances in Optical and Electron Microscopy. Vol. 3. Edited by R. Barer and V. E. Cosslett

The production of vacuolated mitochondria and myelin figures can be markedly reduced with aldehyde perfusion fixation by perfusing a concentrated aldehyde solution before the conventional perfusate. A method is presented for accomplishing this with no practical time delay. Factors causing myelin figures and damaged mitochondria are discussed. Gum acacia helps prevent ‘washed-out’ cells and processes, and seems to aid myelin preservation. A clear solution is necessary for a good perfusion. Techniques for accomplishing this are presented. A concentrated formaldehyde solution perfused as a small volume immediately prior to the conventional perfusate causes alterations of the endoplasmic reticulum, including the subsurface cisterns, and an increase in numbers of spine apparatuses. Evidence is presented by using tubes of gelatin containing Schiff's reagent that formaldehyde is the fastest penetrating aldehyde but that it only fixes about half the distance it penetrates. Acrolein penetrates more rapidly than glutaraldehyde but fixes only as far as formaldehyde. Glutaraldehyde fixes as far as it penetrates.     

Tribological behaviour of polyalphaolefins: Wear and rolling‐contact fatigue tests

Polyalphaolefin (PAO) fluids have become widely accepted as high‐performance lubricants and functional fluids due to certain inherent, and highly desirable, characteristics. One of these characteristics is their low toxicity, which, combined with excellent viscometrics and lubricity, have made low‐viscosity PAO fluids an important component in lubricant formulations. Typical data found in product specifications for lubricants are the kinematic viscosity and the viscosity index. These values do not give enough information with which to choose the optimum lubricant for a lubricated contact. In mechanical systems, rolling, sliding, and rolling/sliding contacts occur, and lubricants have to work optimally under these operating conditions. In this study the rolling‐contact fatigue lives (L₅₀ and L₁₀) of PAOs of different viscosities were experimentally determined. The tests were carried out using a four‐ball machine. Wear tests were also carried out using another four‐ball tester in order to measure the wear‐scar diameter and the flash temperature parameter. The lubricants were characterised by infrared spectroscopy, and the pitting of the balls was observed using scanning electron microscopy.     

Wear of some F.C.C. metals during unlubricated sliding Part III: A mechanical aspect of wear

The relations between the size of wear fragments and the sliding velocity or normal load were studied from a mechanical point of view for the unlubricated wear of nickel. It was found that the size of mean wear fragments has a definite quantitative correlation with the thickness of the plastically deformed substrate layer, irrespective of the experimental conditions. The thickness of the deformed layer is determined by the actual forces working on real contact points, which are dependent on the dynamic properties of the specific sliding system. These mechanical factors govern wear-velocity and wear-normal load characteristics.