基于SolidWorks的机床夹具标准件三维图库的开发

机床夹具标准件图形库系统是制造工艺技术信息系统的重要组成部分.本文根据夹具设计的特点,介绍基于Delphi面向对象编程和数据库等技术的基础上,运用SolidWorks特征化、参数化造型方法及提供的二次开发工具,提出了基于SolidWorks环境下的三维机床夹具标准件库子系统的开发方法,为用户提供一种基于三维建模的快速夹具设计环境.     

基于Visual LISP的二三维标准件图形参数化设计研究

AutoCAD作为一种通用设计软件,它提供了多种二次开发环境,以弥补它在自动绘图方面的不足.同时以GB5780-2000系列六角头螺栓为例,详细说明了如何采用内嵌于AutoCAD的二次开发环境Visual LISP来实现二三维标准件图形的参数化设计,从而为用户提供简便且界面友好的标准件零件自动绘制交互式环境.     

From 2D slices to 3D volumes: image based reconstruction and morphological characterization of hippocampal cells on charged and uncharged surfaces using FIB/SEM serial sectioning

3D imaging at a subcellular resolution is a powerful tool in the life sciences to investigate cells and their interactions with native tissues or artificial objects. While a tomographic experimental setup achieving a sufficient structural resolution can be established with either X-rays or electrons, the use of electrons is usually limited to very thin samples in transmission electron microscopy due to the poor penetration depths of electrons. The combination of a serial sectioning approach and scanning electron microscopy in state of the art dual beam experimental setups therefore offers a means to image highly resolved spatial details using a focused ion beam for slicing and an electron beam for imaging. The advantage of this technique over X-ray μCT or X-ray microscopy attributes to the fact that absorption is not a limiting factor in imaging and therefore even strong absorbing structures can be spatially reconstructed with a much higher possible resolution. This approach was used in this study to elucidate the effect of an electric potential on the morphology of cells from a hippocampal cell line (HT22) deposited on gold microelectrodes. While cells cultivated on two different controls (gold and polymer substrates) did show the expected stretched morphology, cells on both the anode and the cathode differed significantly. Cells deposited on the anode part of the electrode exhibited the most extreme deviation, being almost spherical and showed signs of chromatin condensation possibly indicating cell death. Furthermore, EDX was used as supplemental methodology for combined chemical and structural analyses.