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对几种水解玉米蛋白的酶进行了筛选,确定碱性蛋白酶AlcalaseAF2.4L为最佳酶。以水解度为考察指标通过正交试验对碱性蛋白酶AlcataseAF2.4L水解玉米蛋白的条件进行优化并确定最佳水解条件,并对所制备的短肽的抗氧化性进行初步研究。实验结果表明:碱性蛋白酶在pH值为9.0、温度为50.0℃、酶浓度与底物浓度之比(E/S)为3.0%、固液比为1:18、酶解时间为3.0h的条件下水解度最好为22.80%,总氮回收率为89.58%,所制得短肽的平均肽链长度为3.93。所制得的短肽有较好的抗氧化性,其中对羟自由基的清除率达82.20%。 相似文献
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酶解玉米蛋白制备玉米短肽最佳工艺条件的研究 总被引:1,自引:0,他引:1
酶解玉米蛋白(corn gluten meal,CGM)对生产高营养且易于吸收、高附加值的、具有生物学功能特性的生物产品具有重要意义。本研究通过采用不同的正交试验设计确定了Alcalase AF2.4L和Flavourzyme酶解玉米蛋白的各因素及水平,确定了二次酶解玉米蛋白的最佳酶解条件。 相似文献
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以高水解度水解蛋白为目标,对酶解玉米醇溶蛋白制备短肽的工艺进行了研究。选择了碱性蛋白酶、中性蛋白酶、胃蛋白酶,采用均匀试验设计法对三种酶的酶解工艺进行了考察,同时还对复合酶水解、理化前处理技术的作用进行了探讨。实验结果对三种酶的酶解工艺条件进行了优化;明确了三种酶复合水解时,得到的水解度均显著高于单酶水解结果,且其中以"碱性蛋白酶+中性蛋白酶+胃蛋白酶"顺序的复合酶组合得到的水解度最高达(29.95±0.87)%;考察的加热、添加亚硫酸钠、超声等三种理化前处理技术,对玉米醇溶蛋白水解度没有明显的影响。总而言之,应用合适的蛋白酶及酶解方式,玉米醇溶蛋白水解制备短肽,可以达到较高的水解度。 相似文献
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该文阐述玉米黄粉中各蛋白组分性质及提取方法,指出超声波乙醇法提取醇溶蛋白和超声波辅助碱法提取谷蛋白是目前较有推广应用价值提取法;且玉米黄粉中各蛋白组分提取及应用要根据其性质确定。 相似文献
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Y Victor Wu 《Journal of the science of food and agriculture》2001,81(13):1223-1227
Corn gluten meal with good emulsifying activity and emulsion stability may have potential in food uses. Commercial corn gluten meal from various producers had a pH around 4 and showed no emulsifying activity. Reducing the particle size to below 15 µm or increasing the pH to 6.6 (with particle size above 53 µm) did not improve the emulsifying activity. When the particle size was reduced to below 44 µm and the pH was adjusted to 6.6 or above, good emulsifying activity and good emulsion stability could be obtained. The emulsifying activities of corn gluten meals (<44 µm) from four different producers at around pH 8 ranged from 49.3 to 51.5 and the corresponding emulsion stabilities ranged from 39.7 to 49.5. There was no significant variation in emulsifying activity between pH 6.9 and 7.8 when NaOH, LiOH and KOH were used to adjust the pH, but LiOH‐ and KOH‐adjusted meal at around pH 7.8 showed better emulsion stability than NaOH‐adjusted meal. Good emulsifying activity of corn gluten meal could be obtained in NaCl solutions at sufficiently high pH. Published in 2001 for SCI by John Wiley & Sons, Ltd. 相似文献
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