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1.
目的构建二硫键稳定的抗HIV-1 gp41单链抗体(dsFv)基因原核表达载体,并进行表达和鉴定。方法采用PCR定点突变的方法,构建含二硫键稳定的抗HIV-1 gp41单链抗体突变基因质粒pUC57-d41,BamHⅠ和HindⅢ双酶切后,定向插入pET-28a(+),转化大肠杆菌BL21(DE3),IPTG诱导表达,用SDS-PAGE、Western blot鉴定表达产物。对目的蛋白进行纯化和复性,并进行抗原结合活性及相对稳定性检测。结果重组载体pET-d41经酶切鉴定,证实构建正确。表达产物相对分子质量约为28000,与理论预期值完全相符。目的蛋白最高表达量可占菌体总蛋白的45.48%。经Ni-NTA亲和层析法纯化并复性后,蛋白纯度达95%以上,抗HIV-1 gp41 dsFv具有抗原结合活性,稳定性优于scFv。结论已成功构建了二硫键稳定的抗HIV-1 gp41单链抗体(dsFv)基因原核表达载体,并获得表达,为进一步研究其生物学功能奠定了基础。 相似文献
2.
Wang LX Song H Liu S Lu H Jiang S Ni J Li H 《Chembiochem : a European journal of chemical biology》2005,6(6):1068-1074
C34 is a 34-mer peptide derived from the C-terminal ectodomain of HIV-1 envelope glycoprotein, gp41. The C34 region in native gp41 carries a conserved N-glycan at Asn637 and the sequence is directly involved in the virus-host membrane fusion, an essential step for HIV-1 infection. This paper describes the synthesis of glycoforms of C34 which carry a monosaccharide, a disaccharide, and a native oligosaccharide moiety. The synthesis of the glycopeptide which carries a native high-mannose type N-glycan was achieved by a chemoenzymatic approach by using an endoglycosidase-catalyzed oligosaccharide transfer as the key step. The effects of glycosylation on the inhibitory activity and the helix-bundle forming ability of C34 were investigated. It was found that glycosylation moderately decreases the anti-HIV activity of C34 and, in comparison with C34, glyco-C34 forms less compact six-helix bundles with the corresponding N-terminal peptide, N36. This study suggests that conserved glycosylation modulates the anti-HIV activity and conformations of the gp41 C-peptide, C34. 相似文献
3.
Oliva R Falcigno L D'Auria G Dettin M Scarinci C Pasquato A Di Bello C Paolillo L 《Chembiochem : a European journal of chemical biology》2003,4(8):727-733
Proteolytic activation of the HIV-1 envelope glycoprotein gp160 is selectively performed by the proprotein convertase furin at the C-terminus of the sequence R508-E-K-R511 (site 1), in spite of the presence of another consensus sequence, Lys500-Ala-Lys-Arg503 (site 2). On the basis of the solution structural analysis of the synthetic peptide p498, spanning the gp160 sequence Pro498-Gly516, we previously suggested a possible role of an N-terminal helix in regulating the exposure and accessibility of the gp160 physiological cleavage site, enclosed in a loop. Here we report on the activity and conformation of the 23-residue peptide h-REKR, designed to exhibit a large N-terminal helix, followed by the gp160 native sequence, Arg508-Gly516. h-REKR is digested by furin with high efficiency, comparable to the full native p498. Circular dichroism analyses, in mixtures from pure water to 98 % trifluoroethanol, outline a significant content of helical structure in the peptide conformation. The molecular model obtained from NMR data collected in trifluoroethanol/water, by means of DYANA and AMBER simulations, indeed has helical structure on a large N-terminal segment. Such a long helix does not seem to affect the loop conformation of the C-terminal site 1-containing sequence, which exhibits the same proton chemical shifts already observed for the full native p498. 相似文献
4.
Mzoughi O Gaston F Granados GC Lakhdar-Ghazal F Giralt E Bahraoui E 《ChemMedChem》2010,5(11):1907-1918
The objective of this project was to study the interaction between HR1 and HR2, the stability of the complex formed, and to characterize the antibodies produced against monomeric HR1 and HR2 peptides as well as the HR1-HR2 complex. In this work, HR1 was mimicked by peptide N36, and HR2 was mimicked by peptide C34L and its analogues C34M2, C34M3, and C34D. Whereas C34M2 and C34M3 are partially composed of D-amino acids, C34D has same sequence as C34L, but is assembled entirely of D-amino acids. Using CD analysis, SPR assays, and gel filtration chromatography, we demonstrate the physical interaction between N36 and C34L and its analogues C34M2 and C34M3, but not C34D. We show that the HR1-HR2 complex is formed rapidly (<1?min) and remains stable, as demonstrated by its inability, in contrast to each free peptide, to inhibit the formation of syncytia. To generate antibodies with predetermined specificity against the transiently exposed intermediate that corresponds to the six-helix bundle structure, purified preformed HR1-HR2 complex was used, in parallel with monomeric HR1 and HR2 peptides, as immunogens in mice. Although the produced antibodies recognize total HIV-1 envelope glycoproteins in ELISA, they are unable to neutralize HIV-1-mediated fusion at 37?°C. However, if the incubation with these antibodies is carried out at 27?°C, a temperature that allows stabilization of the transient intermediate complex, anti-peptide antibodies are able to bind their corresponding domains in HeLa cells expressing HIV-1 gp41 in co-culture with HeLa CD4-CCR5/CXCR4 during the dynamic mechanism of membrane fusion. In agreement with the latter results, these antibodies, if previously incubated for 2?h at 27?°C, are able to strongly neutralize HIV-1 entry by membrane fusion, as shown by their ability to block the formation of syncytia. 相似文献
5.
Stigler Rolf-Dietrich; Ruker Florian; Katinger Dietmar; Elliott Graham; Hohne Wolfgang; Henklein Peter; X.Ho Joseph; Keeling Kim; Carter Dan C.; Nugel Elsa; Kramer Achim; Porstmann Tomas; Schneider-Mergener Jens 《Protein engineering, design & selection : PEDS》1995,8(5):471-479
The molecular interaction of the Fab fragment of the human monoclonalantibody 3D6, directed against the transmembrane protein gp41of human immunodeficiency virus (HTV) 1, with its peptide epitopeis characterized by a panel of overlapping peptides, a peptideepitope library and molecular modeling techniques. The sequenceCSGKLICTTAVPW, corresponding to amino acids 605617 ofgp41, was identified as the best binding peptide (KD = 1x10-8mol/1). This peptide served as a starting point to prepare acellulose-bound peptide epitope library in which each residueof the epitope is substituted by all L- and D-amino acids, resultingin 494 epitope peptide variants which were subsequently analyzedfor binding 3D6. The library was synthesized to identify residuescritical for binding and to obtain information about the molecularenvironment of the epitope peptide bound to 3D6. Both cysteineresidues, as well as isoleucine 6, threonine 8 and proline 12,of the epitope were highly sensitive to substitution. Usingthe data obtained from the epitope characterization, as wellas a low-resolution electron density map of a 3D6 Fab-peptidecomplex, a 3-D model of the Fab-peptide complex was generatedby molecular modeling. The modeling experiments predict bindingof the peptide, which is cyclized via the two cysteine residues,to a pocket formed dominantly by the hypervariable loops complementaritydetermining regions CDR3L, CDR2H and CDR3H. 相似文献
6.
Schiavone M Fiume G Caivano A de Laurentiis A Falcone C Masci FF Iaccino E Mimmi S Palmieri C Pisano A Pontoriero M Rossi A Scialdone A Vecchio E Andreozzi C Trovato M Rafay J Ferko B Montefiori D Lombardi A Morsica G Poli G Quinto I Pavone V de Berardinis P Scala G 《International journal of molecular sciences》2012,13(5):5674-5699
The Bridging Sheet domain of HIV-1 gp120 is highly conserved among the HIV-1 strains and allows HIV-1 binding to host cells via the HIV-1 coreceptors. Further, the bridging sheet domain is a major target to neutralize HIV-1 infection. We rationally designed four linear peptide epitopes that mimic the three-dimensional structure of bridging sheet by using molecular modeling. Chemically synthesized peptides BS3 and BS4 showed a fair degree of antigenicity when tested in ELISA with IgG purified from HIV(+) broadly neutralizing sera while the production of synthetic peptides BS1 and BS2 failed due to their high degree of hydrophobicity. To overcome this limitation, we linked all four BS peptides to the COOH-terminus of GST protein to test both their antigenicity and immunogenicity. Only the BS1 peptide showed good antigenicity; however, no envelope specific antibodies were elicited upon mice immunization. Therefore we performed further analyses by linking BS1 peptide to the NH2-terminus of the E2 scaffold from the Geobacillus Stearothermophylus PDH complex. The E2-BS1 fusion peptide showed good antigenic results, however only one immunized rabbit elicited good antibody titers towards both the monomeric and oligomeric viral envelope glycoprotein (Env). In addition, moderate neutralizing antibodies response was elicited against two HIV-1 clade B and one clade C primary isolates. These preliminary data validate the peptide mimotope approach as a promising tool to obtain an effective HIV-1 vaccine. 相似文献
7.
HIV-1 integrase (IN) is composed of three domains, the N-terminal domain (NTD, residues 1-50), the catalytic core domain (CCD, residues 51-212), and the C-terminal domain (CTD, residues 213-288). All the three domains are required for the two known integration reactions. CCD contains the catalytic triad and is believed to bind viral DNA specifically, and CTD binds viral DNA in a nonspecific manner. As no clear evidence has confirmed the involvement of NTD in DNA binding directly, NTD has not been seriously considered and less is known about its function in viral replication. In the current work, using a SPR technology-based assay, the HIV-1 viral DNA was determined to bind directly to NTD with a K(D) value of 8.8 microM, suggesting that the process of preintegrated complex formation for HIV-1 IN might involve the direct interaction of NTD with viral DNA in addition to binding of viral DNA to the catalytic core domain and C-terminal domain. Moreover, such viral DNA/IN binding could be inhibited by the marine product hyrtiosal from the marine sponge Hyrtios erectus with an IC(50) of 9.60+/-0.86 microM. Molecular dynamic analysis correlated with a site-directed mutagenesis approach further revealed that such hyrtiosal-induced viral DNA/IN binding inhibition was caused by the fact that hyrtiosal could bind HIV-1 NTD at Ser17, Trp19, and Lys34. As hyrtiosal was recently discovered by us as a protein tyrosine phosphatase 1B (PTP1B) inhibitor,1 this work might also supply multiple-target information for this marine product, and the verified HIV-NTD/HIV-1 IN interaction model could have further implications for new HIV-1 IN inhibitor design and evaluation. 相似文献
8.
Exploration of potent inhibitors of the HIV-1 gp41 fusion core formation is a promising strategy to discover small-molecule HIV-1 entry inhibitors for the treatment of HIV-1 infection. In this paper, a series of novel betulinic acid-polyphenol conjugates was designed, guided by molecular modeling of the binding of betulinic acid (BA) and phenolic galloyl/caffeoyl groups in the groove on the gp41 N-terminal heptad repeat (NHR) trimeric coiled coil. These conjugates were synthesized via conjugation of galloyl and caffeoyl groups with BA at the C-28 position. Their inhibitory activities of HIV gp41 six-helix bundle (6-HB) formation between the NHR peptide N36 and the C-terminal heptad repeat (CHR) peptide C34 were evaluated with size-exclusion HPLC. Conjugates bearing a galloyl group were found to exhibit four to sixfold higher inhibitory activities than that of parent compound BA, suggesting that they may be exploitable as HIV-1 fusion/entry inhibitors targeting gp41. The docking study on BA and its derivatives suggests that hydrophobic and hydrogen-bonding pockets exist in the groove of the gp41 NHR trimeric coiled coil and that a potent inhibitor should have amphiphilic structures to cooperatively interact with both pockets. This possibility was explored by incorporating both lipophilic and hydrophilic groups into the conjugates in a well-defined orientation to bind with both pockets in the gp41 NHR-trimer. 相似文献
9.
De Pinto V Tomasello F Messina A Guarino F Benz R La Mendola D Magrì A Milardi D Pappalardo G 《Chembiochem : a European journal of chemical biology》2007,8(7):744-756
Mitochondrial porin or VDAC (voltage-dependent anion-selective channel) is the most abundant protein in the mitochondrial outer membrane. The structure of VDAC has been predicted to be a transmembrane beta-barrel with an alpha-helix at the N terminus. It is a matter of debate as to whether this putative alpha-helix plays a structural role as a component of the pore walls or a function in the pore activity. We have synthesised the human VDAC1 (HVDAC1) N-terminal peptide Ac-AVPPTYADLGKSARDVFTK-NH2 (Prn2-20) and determined its structure by CD and NMR spectroscopy. CD studies show that the Prn2-20 peptide exists in aqueous solvent as an unstructured peptide with no stable secondary structure. In membrane-mimetic SDS micelles or water/trifluoroethanol, however, it assumes an amphipathic alpha-helix conformation between Tyr5 and Val16, as deduced from NMR. No ordered structure was observed in dodecyl beta-maltoside. Differential scanning calorimetric measurements were carried out in order to examine the membrane affinity of the peptide. Upon interaction with the negatively charged 1,2 dipalmitoyl-sn-glycero-3-phosphoserine membrane, Prn2-20 exhibited distinctive behaviour, suggesting that electrostatics play an important role. Interaction between the peptide and artificial bilayers indicates that the peptide lies on the membrane surface. Recombinant HVDAC1 deletion mutants, devoid of seven or 19 N-terminal amino acids, were used for transfection of eukaryotic cells. Over-expression of HVDAC1 increases the number of Cos cells with depolarised mitochondria, and this effect is progressively reduced in cells transfected with HVDAC1 lacking those seven or 19 amino acids. The mitochondrial targeting of the deletion mutants is unaffected. The overall picture emerging from our experiments is that the VDAC N-terminal peptide plays a role in the proper function of this protein during apoptotic events. 相似文献
10.
应用 HIV-18 P41抗原决定簇的合成肽作为抗原,建立了合成肽 ELISA 试剂盒,并用于血清HIV—1抗体的初筛试验。其 P/N 值为7.58,阴性平均 OD 值仅0.139,无假阳性和假阴性。用该试剂检测云南边境居民血清807份,其结果与应用进口 ELISA 试剂盒和免疫印迹法所测的结果相同,该试剂可用于HIV—1抗体检测。 相似文献
11.
Dietz J Koch J Kaur A Raja C Stein S Grez M Pustowka A Mensch S Ferner J Möller L Bannert N Tampé R Divita G Mély Y Schwalbe H Dietrich U 《ChemMedChem》2008,3(5):749-755
The interaction of the nucleocapsid NCp7 of the human immunodeficiency virus type 1 (HIV-1) Gag polyprotein with the RNA packaging signal Psi ensures specific encapsidation of the dimeric full length viral genome into nascent virus particles. Being an essential step in the HIV-1 replication cycle, specific genome encapsidation represents a promising target for therapeutic intervention. We previously selected peptides binding to HIV-1 Psi-RNA or stem loops (SL) thereof by phage display. Herein, we describe synthesis of peptide variants of the consensus HWWPWW motif on membrane supports to optimize Psi-RNA binding. The optimized peptide, psi-pepB, was characterized in detail with respect to its conformation and binding properties for the SL3 of the Psi packaging signal by NMR and tryptophan fluorescence quenching. Functional analysis revealed that psi-pepB caused a strong reduction of virus release by infected cells as monitored by reduced transduction efficiencies, capsid p24 antigen levels, and electron microscopy. Thus, this peptide shows antiviral activity and could serve as a lead compound to develop new drugs targeting HIV-1. 相似文献
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13.
Köhler K Lellouch AC Vollmer S Stoevesandt O Hoff A Peters L Rogl H Malissen B Brock R 《Chembiochem : a European journal of chemical biology》2005,6(1):152-161
Cellular signal transduction proceeds through a complex network of molecular interactions and enzymatic activities. The timing of these molecular events is critical for the propagation of a signal and the generation of a specific cellular response. To define the timing of signalling events, we introduce the combination of high-resolution confocal microscopy with the application of small-molecule inhibitors at various stages of signal transduction in T cells. Inhibitors of Src-family tyrosine kinases and actin dynamics were employed to dissect the role of the lymphocyte-specific tyrosine kinase Lck in the formation and maintenance of T cell receptor/CD3-dependent contacts. Anti-CD3epsilon-coated coverslips served as a highly defined stimulus. The kinetics of the recruitment of the yellow fluorescent protein-tagged signalling protein ZAP-70 were detected by high-resolution confocal microscopy. The analysis revealed that at 5 min after receptor engagement, Lck activity was required for maintenance of contacts. In contrast, after 20 min of receptor engagement, the contacts were Lck-independent. The relevance of the timing of inhibitor application provides a pharmacological concept for the maturation of T cell-substrate contacts. 相似文献
14.
Narendra Kumar Ville Nieminen Lars Eric Lindfors Tapio Salmi Dmitry Yu. Murzin Ensio Laine Teemu Heikkilä 《Catalysis Letters》2002,78(1-4):105-110
Cu–H-MCM-41, H-MCM-41 and Na-MCM-41 mesoporous molecular sieve catalysts were synthesized, characterized and investigated in the isomerization of 1-butene to isobutene. Introduction of copper in MCM-41 was found to play a positive role in enhancing the conversion of 1-butene and yield of isobutene and Cu–H-MCM-41 exhibited higher conversion of 1-butene and yield to isobutene than H-MCM-41 and Na-MCM-41 catalysts. The pretreatments of Cu–H-MCM-41 catalyst with synthetic air or hydrogen were observed to influence the 1-butene conversion, yield of isobutene and selectivity to isobutene. Pre-treated with the synthetic air the Cu–H-MCM-41-Ox catalyst exhibited higher conversion of 1-butene, yield of isobutene and selectivity to isobutene than hydrogen pre-treated Cu–H-MCM-41-Red. The reason for such a behavior of Cu–H-MCM-41-Red is the reduction of copper species to metallic form. The X-ray powder diffraction pattern of Cu–H-MCM-41-Red exhibited a peak attributed to the reduction of Cu2+ to Cu0. FTIR spectra of adsorbed pyridine showed the presence of Brønsted and Lewis acid sites in the H-MCM-41, Na-MCM-41 and Cu–H-MCM-41 catalysts. 相似文献
15.
Mekmouche Y Coppel Y Hochgräfe K Guilloreau L Talmard C Mazarguil H Faller P 《Chembiochem : a European journal of chemical biology》2005,6(9):1663-1671
Aggregation of the human peptide amyloid-beta (Abeta) is a key event in Alzheimer's disease (AD). Zinc ions play an important role in AD and in Abeta aggregation. In vitro, Zn(II) binds to Abeta and accelerates its aggregation. In this work we have investigated Zn(II) binding to the synthetic peptide Abeta1-16, which contains the metal-binding domain of Abeta. Cd(II) was used to probe the Zn(II) site. Abeta1-16 bound one equivalent of Zn(II) with an apparent dissociation constant (Kd) of 10(-4) M. This Kd value is in the same range as the Zn concentration needed to precipitate Abeta. Circular dichroism and NMR indicated predominantly random-coil secondary structures of apo-Abeta1-16, Zn(II)-Abeta1-16 and Cd(II)-Abeta1-16, which were all highly dynamic and flexible. The three histidines at positions 6, 13 and 14 were suggested to be ligands to Zn(II) and Cd(II). Evidence that the aspartate at position 1 served as a fourth ligand to Zn(II) and Cd(II) was found at pH 8.7. 111Cd(II) NMR showed a resonance at 84 ppm, in line with a mixed oxygen-/nitrogen-ligand environment. The tyrosine at position 10 could be excluded as a ligand. 相似文献
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18.
L-alpha-amino acids with a nucleobase in the side chain (nucleobase amino acids; NBAs) were used to enhance the function of RNA-binding proteins that recognize structured RNA. These NBAs were utilized in the three-dimensional structure of the protein to enhance RNA binding affinity and specificity as a result of selective recognition of NBAs by RNA bases. NBA units were incorporated at various positions into the HIV-1 nucleocapsid protein NCp7 (residues 1-55), which contains two CCHC-type (Cys-X(2)-Cys-X(4)-His-X(4)-Cys-type; X=an amino acid residue) zinc knuckle domains. The binding ability was evaluated by using the stem-loop (SL)3 region of HIV-1 Psi-RNA. Visible light absorption measurements revealed that two zinc ions bound strongly and quantitatively to the NBA-NCp7 molecule and to the wild-type NCp7 protein. This result indicates that the incorporation of NBA units composed of L-alpha-amino acids did not influence the formation of the specific structure of NCp7. Binding analysis with fluorescein-labeled SL3 RNA revealed that incorporation of NBA units into the NCp7 protein at appropriate positions increased its RNA binding affinity and specificity. An NBA-NCp7 protein that possessed cytosine and guanine NBA units at positions 13 and 46, respectively, showed a binding affinity for SL3 RNA ninefold higher than that of wild-type NCp7 as a result of the specific and cooperative interaction of the NBA units with RNA bases. These results clearly demonstrate that inclusion of NBA units in the three-dimensional structure of an RNA-binding protein is a useful strategy for enhancing the function of the protein. 相似文献
19.
Sussman Fredy; Villaverde M.Carmen; Martinez Luis 《Protein engineering, design & selection : PEDS》2002,15(9):707-711
One of the most successful drug targets against AIDS in thelast decade has been the HIV-1 protease (HIV-1 PR), an enzymethat processes the polyprotein gene products into active replicativeviral proteins. In our quest for a wide-ranging, binding freeenergy function we have extended the solvent accessibility freeenergy predictor (SAFE_p) method, recently developed for peptidicHIV-1 PR inhibitors, to the study of the binding of cyclic urea(CU) HIV-1 PR inhibitors. Our results show that there is a needfor a specific term depicting polar contacts to be added tothe original SAFE_p analytical expression, an outcome not seenin our studies of HIV-1 PR peptidic inhibitors. Nevertheless,despite the higher profile of the electrostatic interactionsin the binding of the CU inhibitors, our analysis indicatesthat CU inhibitor binding is still driven by the hydrophobicentropic contribution, as much as for the peptidic inhibitors. 相似文献
20.
Mansilla F; Knudsen CR; Laurberg M; Clark BF 《Protein engineering, design & selection : PEDS》1997,10(8):927-934
We have mutated lysine 2 and arginine 7 in elongation factor Tu from
Escherichia coli separately either to alanine or glutamic acid. The aim of
the work was to reveal the possible interactions between the conserved
N-terminal part of the molecule, which is rich in basic residues and
aminoacyl-tRNA. The enzymatic characterization, comprising GDP and GTP
temperature stability assays and measurement of nucleotide dissociation and
association rate constants, GTPase activity and aminoacyl-tRNA binding,
shows that position 2 is not involved in aminoacyl-tRNA binding, while
position 7 is necessary to accomplish this activity. Furthermore, arginine
7 seems to play a role in regulating the binding of GTP. The
three-dimensional structure of the ternary complex, EF-Tu:GTP:Phe-tRNAPhe,
involving Thermus aquaticus EF- Tu and yeast Phe-tRNA(Phe), shows that Arg7
is in a position which permits salt bridge formation with Asp284, thus
binding the N-terminus tightly to domain 2. We propose that this
interaction is needed for aminoacyl-tRNA binding, and also for completing
the structural rearrangement, which takes place when the factor switches
from its GDP to its GTP form.
相似文献