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1.
菊粉酶酶解菊芋提取液的试验 总被引:2,自引:0,他引:2
报道黑曲霉AL-154菊粉酶产生和酶解菊芋提取液的适宜条件:5%菊芋提取液,2%玉米浆、0.3%酵母膏的基本培养基,该酶活力达146.4u/ml,是适培养条件为:PH4.5-5.0,30℃,时间48h,酶解菊芋提取液的最适工艺条件为:PH5.0,60℃,底物总糖浓度为10-20%,酶用量3.0u/g菊糖,酶解时间10h,底物降解率达97.2%,酶解产物中果糖占总糖的86.1%。 相似文献
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克鲁维酵母突变株UV-G-40-3菊粉酶性质的研究 总被引:6,自引:0,他引:6
研究了克鲁维酵母突变株(Kluyveromyces-UV-G-40-3)所产菊粉酶的分布为胞外酶∶胞壁酶∶胞内酶比是5.7∶1.6∶1。该酶S/I为5.3,最适温度为50℃,最适pH为4.5,在50℃以下、pH4.5~8的范围内比较稳定,4℃贮存稳定性好,14d后仍保持76%活力,为外切型菊粉酶,酶解粗菊糖(洋姜提取液)活性为纯菊糖的4倍。 相似文献
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菊粉酶产菌株的诱变选育及产酶条件的研究 总被引:2,自引:1,他引:1
以克鲁维酵母野生为出发菌株,经紫外筛选选到一株产菊粉酶较突变株UV-G-40-3,并对其进行了发酵条件的研究,在菊糖3%、蛋白胨3%、酵母膏1%、PH5.5、36℃,装量30ml/300ml,240r/min摇瓶培养33h,酶活比野生株提高近一倍,为13.9U/ml。 相似文献
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菊粉酶产酶菌株的诱变选育及产酶条件的研究 总被引:4,自引:0,他引:4
以克鲁维酵母(Kluyveromyces)野生株为出发菌株,经紫外线处理筛选到一株产菊粉酶较高的突变株UV-G-40-3,并对其进行了发酵条件的研究,在菊糖3%、蛋白胨3%、酵母膏1%、pH5.5、36℃,装量30ml/300ml,240r/min摇瓶培养33h,酶活比野生株提高近一倍,为13.9U/ml。 相似文献
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在前文基础上,利用V=11m^3传统发酵罐改造为并流型固定床反应器,进行木薯干原料固定化增殖酵母酒精发酵工业性试验。糖化醪糖化率Ys≥50%,载体填充率80L.gel/m^3反应器,发酵时间T16.5hr,成熟醪乙醇浓度9.22%,总糖利用率Uw=92.7%,糖醇化系数YE/SP=0.4717,反应器容积乙醇产率PV=4.5kg,Et-PH/m^3.hr载体乙醇产率Pc=58.18kgEt-OH/ 相似文献
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几丁质固定化无花果蛋白酶的研究 总被引:3,自引:0,他引:3
载体几丁质通过甲酸和戊二醛活化共价偶联无花果蛋白酶,固定化反应在给酶量为1.0mg/g载体,pH7.5,4℃进行15h。制备的固定化酶表观Km值(酪蛋白)为0.95mg/ml,溶液酶的Km值为0.38mg/ml,固定化酶的最适pH范围变宽,由溶液酶的最适pH7.5-7.8变为在pH6-8范围内酶活性保持稳定;固定化酶的最适温度由溶液酶的60℃变为37℃。重复水解酪蛋白7次后,固定化酶保持原酶活性5 相似文献
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酶法水解文蛤肉的研究 总被引:10,自引:2,他引:10
以海产文蛤肉为原料,用酶水解获得复合氨基酸水解液。水解液中含有18种氨基酸,牛磺酸,多种维生素及微量元素,酶水解的最适条件为:水解时间4-6h、温度50-55℃,初始pH7.0,酶添加量1.5-2.0%。酶解后,游离氨基酸含量可达54.62mg/g鲜文蛤肉,其中必需氨基酸占50%以上,牛磺酸为266mg/g鲜文蛤肉,蛋白质的水解率为56.78%。 相似文献
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采用硫酸铵分级沉淀、透析脱盐、DEAE-Cellulose离子交换色谱等分离纯化技术,从Aspergillus ficuum菌株混合酶系中分离得到4种菊粉酶组分,应用薄层层析法分离各组分水解菊粉的产物,发现其中3种组分主要含外切菊粉酶,一种主要含有内切菊粉酶。进一步对所得到的内切菊粉酶组分酶解菊粉制备低聚果糖进行了研究,研究了底物浓度、加酶量、反应温度和反应pH对低聚果糖制备的影响,确定其最适反应条件为:底物浓度50g/L、加酶量10U/g底物,反应温度45℃,反应PH6.0。在此条件下反应72h,菊粉酶解率达74%,低聚果糖得率可达50%以上,酶解产物以DP2~DP4为主。 相似文献
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黑曲霉Aspergillus niger No.26菊粉酶的研究 总被引:10,自引:2,他引:10
通过筛选菌种,分离出3株菊粉酶活力较高的菌株,对其中No.26菌株经过培养基组成的研究,酶活力达37.1u/ml。比原活性6.7u/ml提高5.5倍。经纸上层析鉴定,在菊粉水解过程中,果糖逐步增加,未见中间产物生成,并有很强的水解蔗糖为果糖和葡萄糖,以及水解棉子糖为果糖和蜜二糖的能力。因此该酶主要为外切型菊粉水解酶。该酶在55℃以下较稳定,最适pH为3~5,其低而宽的pH范围有防止微生物污染的能力,具备工业应用的良好性能。 相似文献
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研究了用黑曲霉A1,A15和酵母Y7固态发酵柠檬酸渣生产多酶蛋白饲料。在30℃的最适温度下,3株菌种混合进行固体发酵,粗蛋白增加量(绝干)约为16%-18%,酸性蛋白酶活性5139u/g,纤维素酶活性达61u/g,试验结果表明;该酸性蛋白酶在100℃加热1h有80%的酶存活,系耐高温酸性蛋白酶。 相似文献
11.
Dead cells of Kluyveromyces marxianus having inulase (β-D-fructo fructanohydrolase E.C.3.2.1.7) activity were immobilized in alginate beads and used as a biocatalyst in a packed bed reactor and a stirred batch reactor. Fructosans of Jerusalem artichoke tubers, after extraction, were utilized for continuous or semi-continuous production of fructose. In a bed reactor packed with 100 ml of beads, a volumetric productivity of 36 g/l/hr total reducing sugars was obtained with 98% substrate conversion. When operated continuously for 30 days, a half life of 28 days was observed for the biocatalyst. Using artichoke extract containing 20% fructan solution, 98% conversion could be achieved in a batch reactor in 20 hr. Repeated cycling of beads resulted in considerable loss of catalyst from the reactor and subsequent loss in catalytic activity, thus giving a half life of only 14 days. 相似文献
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The yeast Saccharomyces cerevisiae ATCC 36859, which preferentially utilizes glucose in glucose-fructose mixtures, was immobilized and used for the continuous production of very enriched fructose syrup. A syrup containing fructose as 99% of the reducing sugars was produced from a 10.1% w/v glucose and 9.8% w/v fructose mixture at a dilution rate of 0.106 h−1 . Later in this process when the dilution rate was increased to 0.366 h−1 the ethanol productivity was 12.7 g 1−1 h−1 . This is 16% less than the value attained in a similar medium containing only glucose as the carbohydrate. The fructose content was also increased from 55 to 95% of the reducing sugars in a food grade mixture of high fructose corn syrup and Jerusalem artichoke juice. Purification of the product with activated carbon and ion-exchange resin produced a stable, colourless and very enriched fructose syrup suitable for human consumption. 相似文献
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Non-viable immobilized cells of Kluyveromyces marxianus in alginate beads having inulase (ß-2,1-fructan fructano-hydrolyase E.C.3.2.1.7) acitivity were used as biocatalyst in a packed bed reactor. Extracts of Jerusalem artichoke tubers were contacted with the biocatalyst for continuous conversion of the fructan component to fructose. In a bed reactor packed with 100 mL of beads, a volumetric productivity of 136 g/L/hr total reducing sugars was obtained with 98% substrate conversion. When operated continuously for 30 days, a 55% loss in the original activity was observed, giving a half life for the biocatalyst of 28 days. 相似文献
15.
使用产菊粉酶的酿酒酵母可实现一步法发酵菊芋生产乙醇.该试验以菊芋汁为发酵培养基,研究了起始糖浓度、无机盐、温度、种子培养基、接种量和氮源对高菊粉酶活酿酒酵母Y05-步发酵菊粉生成乙醇的影响.试验结果表明:(1)菊芋汁中最适起始总糖浓度为250g/L.菊芋汁含有的较多无机盐对乙醇发酵不但没有抑制作用,反而有促进作用;(2)最适乙醇发酵温度为37℃;(3)种子培养基中使用菊粉做为碳源有利于酿酒酵母Y05产生较多菊粉酶,进而促进其乙醇发酵;(4)最适乙醇发酵接种量为15%;(5)尽管菊芋汁是一个良好乙醇发酵培养基,但补加氮源仍是必要的.补加5.0g/L玉米浆可显著酿酒酵母Y05的提高总糖利用率、最终乙醇浓度和乙醇得率. 相似文献
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Edward R Farnworth 《Journal of the science of food and agriculture》1994,64(2):217-221
Weaned pigs were fed diets with added Jerusalem artichoke for 28 days. In a first trial, the pigs received raw tubers in addition to their normal ration. In a second trial, the pigs received dried Jerusalem artichoke (10–60 g kg-1) in the diet. Pigs eating the raw tuber had significantly increased body weight gains due to the consumption of the raw tuber, but significantly decreased feed efficiency. Adding dried Jerusalem artichoke to the diet significantly increased weight gains. Feed efficiency was improved, but not significantly. Faecal valeric acid concentration was significantly increased in animals eating Jerusalem artichoke. 相似文献
20.
H. Klaushofer 《Starch - St?rke》1986,38(3):91-94
On the Biotechnology of Plants with Fructosan Content. Inulin, as to its chemical nature, is a polyfructosane. Fructosanes (fructanes) are hardly less abundant in nature than starch. However, there are only few plants with sufficient fructosane content for technological utilization. The most important of them are Jerusalem artichoke and chicory. At first this paper deals with inulin and the homologous series of fructosanes. The importance of Jerusalem artichoke and chicory as agricultural crops and their respective peculiarities are pointed out as far as they are relevant for their use as technical source materials. Inulin containing plants are of interest with respect to the production of fructose and fructose syrup and as energy plants (production of ethanol). Chicory is particularly suitable as source material for fructose-(syrup-)production, Jerusalem artichoke mainly for the production of ethanol, although principally both product groups can be obtained from chicory roots and from topinambur bulbs. If inulin containing raw materials are used for ethanol production, it's of interest that yeast invertase splits polyfructosanes of higher molecular mass considerably more slowly than inulinase and yields are less satisfactory with S. cerevisiae and increasing inulin content in the mash. If yeast types are used containing inulinase and invertase, e. g. Kl. fragilis and marxianus, satisfactory fermentation can be obtained even with these types of mashes. 相似文献