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本文介绍了一类作用机制较特殊的新农药--苯甲酰基苯基脲类杀虫剂的研究近况和主要品种的制备方法。 相似文献
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微胶囊技术在农药剂型中的应用 总被引:42,自引:1,他引:41
微胶囊剂是农药剂型中技术含量最高的一种,它将成为今后农药新剂型的发展方向。本文概述了微胶囊剂所具有的优点和功能,着重介绍了界面缩聚法和就地界面缩聚法制备农药微胶囊剂的基本工艺,微胶囊的控制释放机制和当前已经商品化的各种制剂。 相似文献
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《现代农药》2015,(2):54
<正>日前,第五届中华优秀出版物奖评选结果揭晓并颁奖,由南开大学元素有机化学所杨华铮教授等编著,化学工业出版社出版的大型农药专著——《现代农药化学》获得第五届中华优秀出版物(图书)奖。农药品种及其生产过程和安全使用的"绿色性"要求,已经形成21世纪农药研究的时代特征。新农药的研究已经进入开发超高活性及环境友好产品的"绿色生态化"时代。通过探讨农药与作用靶标间的相互作用来开展新农药创制的"生物合理的"农药分子设计策略正成为新的研究热点和主流,这对于农药进入"绿色生态化"时代具有重要的意义。在这种新形势下,传统的以化学合成为主线介绍农药化学的专业书籍已远不能满足需要,亟需一本从新的角度来介绍农药基本内容的专业图书。《现代农药化学》首次以农药作用机制为"主线",系统 相似文献
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1前言早期的农药用量大、毒性高、对环境污染大、抗性严重,有破坏自然平衡的危险。人类的目的是控制与限制病虫草害对粮食生产的危害,而不是消灭生物物种。由早期研究工作中类同作用机制农药分子结构的改进、优化到现代不断推出多样性作用机制化学新农药是目前农药研究中的明显特点。现代农药以不给自然界带来异源物质的危害为方针,出现了超高效、低残留、低污染的特异性农药。2—氯-5-氯甲基吡啶就是新型烟碱类农药吡虫啉、吡虫清、噻虫啉等的重要中间体,同时也是医药工业的重要原料。 相似文献
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目的用5-溴脱氧尿嘧啶核苷(BrdU)标记家兔皮肤成纤维细胞,确定最佳标记方法,探讨其作为成纤维细胞示踪方法的可行性。方法取12~16周龄健康中国大耳白兔颈部皮肤,胶原酶消化,分离并培养成纤维细胞。取第3代细胞,加入终浓度分别为2.5、5、10和15μg/ml的BrdU共培养,MTT法检测细胞的生长情况。分别用2.5、5、10和15μg/ml的BrdU标记第3代细胞,标记6、12、24和48h后,采用免疫荧光法检测各组细胞的标记阳性率。以最佳剂量及时间标记家兔成纤维细胞,与壳聚糖-甘油磷酸钠水凝胶混合后,注入兔颈内动脉,1周后,免疫荧光法观察植入细胞。结果加入BrdU48h内,各浓度的BrdU对家兔成纤维细胞生长的影响均不明显。标记剂量及标记时间均可影响标记率,但标记时间的影响更大。5μg/ml剂量标记24h,标记率可达(94.50±2.08)%,再加大标记剂量或延长标记时间,标记率提高不明显。以该方法标记的家兔成纤维细胞注入家兔颈内动脉1周后,可在兔颈内动脉观察到大量标记细胞。结论BrdU对家兔成纤维细胞的最佳标记方法为5μg/ml标记24h,该方法对细胞影响小,标记率高,适用于成纤维细胞体内示踪。 相似文献
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Dr. Yuxin Fang Prof. Dr. Peng Zou 《Chembiochem : a European journal of chemical biology》2023,24(8):e202200745
Investigating the subcellular organization of biomolecules is important for understanding their biological functions. Over the past decade, proximity-dependent labeling methods have emerged as powerful tools for mapping biomolecules in their native context. These methods often capitalize on the in-situ generation of highly reactive intermediates for covalently tagging biomolecules located within nanometers to sub-micrometers of the source of labeling. Among these, photocatalytic proximity labeling methods achieve precise spatial and temporal control of labeling with visible light illumination. In this review, we summarize the mechanisms and applications of existing photocatalytic proximity labeling methods and discuss future opportunities for improving the method. 相似文献
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Transglutaminase-mediated N- and C-terminal fluorescein labeling of a protein can support the native activity of the modified protein 总被引:1,自引:0,他引:1
Fluorescein and its analogs are among the best fluorophores to label proteins and the labeling generally involves chemical modification of a translated protein. Using this methodology, labeling at a specific position remains difficult. It is known that the guinea pig liver transglutaminase (TGase)-catalyzed enzymatic modification method can allow terminal-specific fluorophore labeling of a protein by monodansylcadaverine. However, native activity of the fluorescent protein has not been investigated so far, nor has direct comparison between the chemical modification and the TGase-catalyzed modification been attempted. Therefore, we compared the possibility of fluorescein labeling via chemical labeling and via TGase-catalyzed modification. The latter method was found to be very practical and overcame some of the problems associated with the specificity of the former; fluorescein was covalently attached only to the N- or C-terminal site of glutathione S-transferase when the reaction was catalyzed by TGase and the resulting labeled protein completely retained its native activity. The TGase-mediated labeling occurred not only at room temperature but also at 4 degrees C to the same extent, which is more desirable for preventing the inactivation of proteins. 相似文献
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对国家食品药品监督管理总局发布的防晒化妆品防晒效果标识要求进行了总结与阐释,将新规与旧规进行对比,详细列举了新规下防晒产品防晒指数(SPF)的标识方法,并对法规过渡期内防晒化妆品防晒指数的变更申请情况予以说明。 相似文献
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Site‐Specific Incorporation of Chemical Fluorescence on Live Enterovirus‐71 Virion by Using an Organometallic Palladium Reagent To Monitor Virus Entry
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Dr. Yaxin Wang Jingwei Liu Lin Cao Wenjing Wang Dr. Yuna Sun Dr. Zheng Yin Dr. Zhiyong Lou 《Chembiochem : a European journal of chemical biology》2018,19(14):1465-1470
Imaging live virus to monitor the viral entry process is essential to understand virus–host interactions during pathogen infection. However, methods for efficient labeling of live viruses, in particular labeling non‐enveloped viruses and tracing virus entry processes, remain limited. Recently, labeling by using organometallic palladium reagents has provided a highly efficient and selective way to bioconjugate cysteines of virus proteins. Here, site‐specific bioorthogonal labeling mediated by an organometallic palladium reagent on the surface of live enterovirus‐71 (EV71) was used to visualize its entry into live cells. In contrast to currently used immunofluorescence and membrane‐anchored dyes, this site‐specific and quantitative labeling of live EV71 allows temporal imaging of its entry into host cell membranes on the timescale of seconds with little negative impact on its virulence. This method revealed details of EV71 virus entry and has broad applicability for monitoring virus entry that is difficult to assess by using conventional protein‐labeling approaches. 相似文献