Occurrence of giant fibers in muscles from wild pigs native to the United States

The semimembranosus (SM) and triceps brachii (TB) muscles from wild pigs native to Florida. USA, were evaluated for histological characteristics. All three fiber types were present in both muscles in varying proportions, reflecting differences between tonic and phasic functioning muscles. Typical fiber arrangements were observed with red fibers grouped in clumps and surrounded by white fibers. The triceps brachii muscle contained a greater percentage of βR and αR fibers and possessed more giant fibers than the SM muscle which contained more αW fibers. The giant fibers observed resembled βR or αR fibers. The presence of hypertrophied fibers in the muscles from these wild pigs suggests that the 'giant fiber syndrome' is an intrinsic susceptibility for muscle fiber enlargement in these animals and not necessarily associated with breeding for muscularity since the carcasses from these pigs contained very small muscles and very little fat.     

Elastin in bovine Semitendinosus and Longissimus dorsi muscles

The structural organization of elastin present in bovine semitendinosus and longissimus dorsi muscles was examined using light and scanning electron microscopy. Qualitatively, both muscles were found to have the same organization of the two structural forms of elastin present.

Coarse elastin fibres (5–10 μm diameter) were found in the epimysium and perimysium, aligned with the long axis of the muscle fibres. In addition, finer elastin fibres (1–2 μm diameter) were found in the epimysial and perimysial sheets of collagen. These finer elastin fibres approximated the course of the collagen fibres, i.e. at an angle to the muscle fibre long axis.

Quantitatively, the semitendinosus muscle epimysium and perimysium contained very heavy deposits of the coarse elastin fibres and nearly every sheet of perimysium, even the thinest, contained the fine elastin fibres. By comparison, less of the coarse elastin was present in the longissimus dorsi epimysium and it was quite rare in the perimysium. The finer elastin fibres were, however, reasonably common.

The significance of this structural arrangement is discussed in relation to muscle function and to meat toughness assessment.     

Histochemical properties of fibre types in muscles of wild and domestic pigs and the effect of growth rate on muscle fibre properties

This study reports the results of a comparison of the histochemical properties of various types of porcine muscles in wild and domestic pigs. In domestic pigs, the influence of growth rates on these properties were studied in five muscles: longissimus dorsi (LD), semimembranosus (SM), gluteus superficialis (GS), infra spinam (IS) and masseter (MAS). The growth rate was expressed as: (i) live weight (LW) at 165±2 days and (ii) daily LW gain from day 88 to day 165 (DG) on the experimental diet. LD, SM, GS and IS of wild pigs were found to contain a higher area percentage of oxidative type IIA fibres (type IIA%(area)) and a lower percentage area of glycolytic type IIB fibres (type IIB%(area)) than the same muscles of domestic pigs. The capillary density in the light muscles (LD, SM, GS) of wild pigs was twice that of domestic pigs, indicating higher oxidative capacity. In domestic pigs the cross sectional area of type IIB fibres (CSA(IIB)) was markedly larger than the cross sectional area of type I (CSA(I)) and IIA (CSA(IIA)) fibres. The average fibre cross sectional area (CSA(fibre)) was about the same in the muscles of wild and domestic pigs except in LD and SM, where the average fibre cross sectional area was approximately 25% smaller in wild pigs than in domestic pigs. This difference was caused by the large cross sectional area of type IIB fibres in the light muscles of domestic pigs. In the light muscles of domestic pigs, the cross sectional area of type IIA fibres increased most with increasing growth rate. Growth rate influences muscle fibre properties only in light muscles, not in dark muscles.     

Variation in haem pigment concentration and colour in meat from British pigs

Variation in chemically determined total haem pigment concentration and instrumentally determined colour was examined in 223 samples of M. longissimus dorsi (LD) representative of the majority of slaughter pigs currently produced in the UK. Whether pigs were sired by White (Large White or Landrace) or Meat-line boars did not affect any measured characteristic but source breeding company influenced total haem pigment concentration (P < 0·01). Haem pigment concentration was higher in muscles from gilts, compared with castrates, boars being intermediate. Gilts also had darker muscles, based on EEL Reflectance values (P < 0·05), and lower hue values (P < 0·05). When compared with animals fed ad-libitum, restricted-fed pigs had higher concentrations of muscle haem pigment (P < 0·001) and this resulted in meat that was slightly darker (P < 0·05), despite having lower ultimate pH (pHu) (P < 0·05), and had a lower hue value (P < 0·001). Measurements of reflectance, total soluble protein and pHu indicated that differences in the incidence of potentially pale, soft, exudative or dark, firm, dry muscle were unlikely to be important contributors to variation in the colour of the meat in this study.     

A Comparison of the Light and Dark Portions of a Striated Muscle

SUMMARY— The levels of physio'ogically related muscle constituents were determined in the light (white) and dark (red) portions of a striated muscle from the pig (SW domcsticus). Myoglobin level, percent red fibers and succinic dehydrogenase activity were two-fold higher in the semitendinosus dark portion whereas ADP and inorganic phosphate levels were similar in both portions. Phosphorus levels were higher and sodium levels lower in the semitendinosus light portion than in the semitendinosus dark portion. Zinc and iron contents were greater in the dark portion than in the light portion; calcium, nickel, boron and potassium levels were similar in both portions. The semitendinosus light portion also had more lipid and more sarcoplasmic nitrogen than did the semitendinosus dark portion. These data suggest that the light (white) and dark (red) portions within the semitendinosus have physio-chemical properties similar to uniformly white and uniformly red muscles, respectively.     

Comparisons of longissimus muscle metabolic enzymes and muscle fiber types in Korean and western pig breeds

We compared differentially expressed genes and muscle fiber types in the longissimus muscles of Korean native pigs (KNP) and the western meat-producing breeds Landrace and Yorkshire. The KNP breed exhibited a higher muscle fat content and more red meat color as determined by the a^* (redness) value (P < 0.01) and b^* (yellowness) value (P < 0.05) compared to the western breeds. Using differential display RT-PCR, we detected two genes that were differentially expressed in skeletal muscle among the pig breeds. These genes were identified as NADH dehydrogenase subunit 1 and ATPase subunit 6 by cloning and sequencing analysis. Both of these genes are involved oxidative phosphorylation and therefore energy metabolism. The genes were more highly expressed in the KNP breed than in the other breeds, indicating that KNPs exhibit more oxidative metabolism than do the western breeds. We also analyzed the mRNA levels of myosin heavy-chain isoforms such as type I (oxidative), type IIb (glycolytic), and types IIa and IIx (intermediate) fibers using real-time RT-PCR. The mRNA levels of oxidative and intermediate fibers were elevated in the KNP breed, whereas the glycolytic fibers were more highly expressed in the Landrace and Yorkshire pigs. These results suggest that the elevated expression of the oxidation-related metabolism genes NADH dehydrogenase and ATPase is related to meat quality as indicated by a higher content of oxidative fibers and muscle fat, as well as redder meat color.     

Identification and characterization of pigs prone to producing 'RSE' (reddish-pink, soft and exudative) meat in normal pigs

RSE (reddish-pink, soft and exudative) meat was investigated using pigs of three different halothane genotypes. A significantly lower pH_1h, value was observed in RSE compared with that of RFN (red, firm and non-exudative) -meat, both of which have values higher than 6.0 at 1 hr post-mortem. Drip loss (%) in RSE-meat was ≥7%, which was twice that of RFN-meat. Normal values for fibre optic probe and Minolta L and a were observed for RSE-meat. RSE-meat could be derived from NN and Nn pigs, and its formation could be induced from RFN-prone pigs by poor post-slaughter management. Pigs expected to produce RSE-meat were identified using small biopsy samples of M. longissimus dorsi (LD). Predicted RSE-meat in live pigs was confirmed by post-mortem assessments of meat quality using LD muscle. With NN Landrace-Yorkshire × Duroc pigs, 15.6% were identified to be RSE-prone in live pigs, and a further 6.7% RSE was induced after slaughter from RFN pigs. The rate of glycolysis determined from biopsy LD samples and at 1 hr post-mortem (pH_1h) were significantly (p < 0.001) faster in RSE than in RFN-prone pigs, but significantly slower than those of PSE-prone pigs. Good correlations (p < 0.001) were observed between biopsy fluid (F) values, an indicator of water-holding capacity (WHC), and drip loss (r = 0.652) from post-mortem LD muscle, and between biopsy pH (F), an indicator for the rate of glycolysis, and F (r = −0.828). These results show that the skeletal muscle test using biopsy LD muscle could be employed to reduce the incidence of RSE-meat.     

Fatty acid profiles and lipid oxidation in beef steer muscles from different anatomical locations

Beef muscles at four different anatomical locations (longissimus dorsi, LD; psoas major, PM; semimembranosus, SM; semitendinosus, ST) were excised 24 h post-mortem from each of 12 steer carcasses and analyzed for total lipids, fatty acid proofiles and lipid oxidation catalysts. Also, the accumulation of thiobarbituric acid (TBA)-reactive substances in ground muscles stored at 4°C was determined. Total lipids and fatty acid composition of total lipid extracts were similar among the muscles from different locations. The microsomal enzymic lipid peroxidation activity was higher for the ST than for other muscles whereas total heme pigment was lower for the ST than for others. The nonheme iron was higher for the PM and SM than for the LD and ST. The accumulation of TBA-reactive substances in stored, raw ground muscle was highest for the PM and lowest for the LD. TBA values of ground muscle samples were positively correlated with heme pigment content and microsomal enzymic lipid peroxidation activity while not correlated with nonheme iron content. It also was positively correlated with the percentage of polyunsaturated fatty acids.     

Quality attributes of major porcine muscles: A comparison with the Longissimus Lumborum

Quality attributes of the longissimus lumborum (LL) were compared to the attributes of the muscles semimembranosus (SM), rectus femoris (RF), biceps femoris (BF), gluteus medius (GM) and the lateral portion of the semitendinosus (ST) in the ham, the psoas major (PM) in the loin and the supraspinatus (SS), infraspinatus (IS) and triceps brachii (TB) in the shoulder. Carcasses were allocated to quality groups based on measurements of muscle exudate and lightness (CIE-L^*) in the LL. The SM, BF, GM and ST in the ham were similar to the LL (P > 0·05) in meat ultimate pH (pHu) and exudate and the GM, ST and LL were similar (P > 0·05) to the LL in lightness. The TB, RF, SS, PM and IS were similar (P > 0·05) in exudate, lightness and pHu and compared to the LL, were less exudative (P < 0·05) and darker (P < 0·05). When the LL was classed pale, and exudative, the GM, BF, SM and ST were also pale and exudative but the other muscles were darker and non-exudative. When the LL was classed dark and non-exudative, all other muscles were dark, non-exudative and the pHu was high. Therefore, the LL could serve as a reliable indicator of colour and exudate for all porcine musculature when the condition was dark and non-exudative (i.e. DFD). However, when the condition was pale and exudative (i.e. PSE), then the LL would serve as a reliable indicator only for the four major ham muscles, excluding the RF.     

Identification of halothane genotypes by calcium accumulation and their meat quality using live pigs

The three halothane genotypes (NN, Nn, and nm) were identified by measuring the capacity for Ca²⁺ accumulation by sarcoplasmic reticulum in whole muscle homogenate preparations of M. longissimus dorsi with a Ca²⁺ specific electrode at 35°C. Significant differences (P < 0·001) in deterioration (%) of Ca²⁺ accumulation, 12% for NN, 35% for Nn, and 81% for nn pigs, were observed after ageing the whole muscle homogenate preparations for 24 h in ice.

Predictions of meat quality in live pigs (n = 34) based on the values for water-holding capacity, assessed as fluid (g/0·5 g wet wt LD), and pH (fluid) by using small biopsy LD samples (Cheah et al. 1993) were performed on all the halothane genotypes. The halothane genotype NN (n = 11) showed a fluid value of 0·37 ± 0·01 and a pH (fluid) value of 6·62 ± 0·03 as compared with 0·61 ± 0·02 and 5·84 ± 0·04, respectively, for the halothane genotype nn (n = 13). The Nn pigs (n = 10) showed fluid (0·49 ± 0·03) and pH (fluid) (6·19 ± 0·11) values between those values observed for the two homozygotes (NN and nn). Predictions of meat quality in live pigs from biopsy LD muscles were confirmed from assessments on post-mortem LD muscles based on pH₁ and fibre optic probe (FOP) measurements.

The extent of deterioration (%) in Ca²⁺ accumulation showed high correlations with fluid (r = −0·861) and pH (fluid) (r = −0·831) in the biopsy LD samples, and with pH₁ (r = 0·663), FOP (r = −0·812), and drip (%) loss (r = −0·777) in the post-mortem LD samples.     

Muscle fiber type characterization and myosin heavy chain (MyHC) isoform expression in Mediterranean buffaloes

This study aimed to evaluate myosin heavy chain (MyHC) isoform expression and muscle fiber types of Longissimus dorsi (LD) and Semitendinosus (ST) in Mediterranean buffaloes and possible fibers muscles modulation according to different slaughter weights. The presence of MyHC IIb isoforms was not found. Only three isoforms of MyHC (IIa, IIx/d and I) were observed and their percentages did not vary significantly among slaughter weights. The confirmation of the presence of hybrid muscles fibers (IIA/X) in LD and ST muscles necessitated classifying the fiber types into fast and slow according to their contractile activity, by m-ATPase assay. For both muscles, the muscle fiber frequency was higher for fast than for slow fibers in all weight groups. There was a difference (P<0.05) in the frequency of LD and ST muscle fiber types according to slaughter weights, which demonstrate that the slaughter weight influences the profile of muscle fibers from buffaloes.     

Characterization of French and Spanish dry-cured hams: influence of the volatiles from the muscles and the subcutaneous fat quantified by SPME-GC

The influence of the volatile compounds on the characterization of Spanish and French dry-cured hams was studied. Thirty volatiles were quantified in each one of four locations (biceps femoris, semimembranosus and semitendinosus muscles and subcutaneous fat) of 29 dry-cured hams by solid-phase microextraction gas-chromatography (SPME-GC). The Brown–Forsythe univariate test allowed determination of the volatiles that individually could characterize (p < 0.05) the samples by their geographical origin (France, Spain) and breed type (Iberian, white). Stepwise linear discriminant procedure, under very strict conditions (F-to-Enter for a F-distribution > 0.95), then selected the most remarkable volatile compounds. Four compounds from the subcutaneous fat (methyl benzene and octanol) and the semitendinosus muscle (2-butanone and 2-octanone) allowed 100% correct classifications by geographic origin. On the other hand, only two compounds from the subcutaneous fat (octanol) and the biceps femoris muscle (3-methyl 1-butanol) correctly classified all the samples by the breed type. The ability of these variables to classify the samples was checked by the unsupervised procedure of principal components.     

Physicochemical characteristics of three muscles from free-range reared Iberian pigs slaughtered at 90 kg live weight

Three muscles from free-range reared pigs with different metabolic pattern were studied. m. Masseter (M), m. Longissimus dorsi (LD) and m. Serratus ventralis (SV) the first having an oxidative metabolism and the other two an intermediate and glycolytic metabolism. m. Masseter contained the highest content of myoglobin (M: 6.65 mg/g, LD: 3.00 mg/g and SV: 3.64 mg/g; P<0.001) and exhibited the highest CIE a* (M:17.10, LD: 14.83, SV:15.34, P<0.001) and C* (M: 17.95, LD: 15.61, SV: 15.54, P<0.001) values. m. L. dorsi and S. ventralis contained a higher intramuscular fat content than m. Masseter (M: 2.26 g/100 g muscle, LD: 4.79 g/100 g muscle, SV: 3.52 g/100 g muscle, P=0.001) and lower amounts of phospholipids (M: 0.33 g/g imf, LD: 0.12 g/g imf, SV: 0.19 g/g imf; P<0.001). Fatty acid profiles from total intramuscular fat and lipid fractions, neutral lipids and polar lipids, significantly differed among muscles, there being a higher content of unsaturated fats (especially in the C18:2 and C20:4 percentages) in the m. Masseter than in the other two muscles analysed. Comparatively, muscles from 90 kg live weight Iberian pigs contained more fat and heme pigments and were redder than those from the commercial pig crosses usually produced.     

The buffering capacity of porcine muscles

The aim of this study was to investigate the buffering capacity (BC) of five porcine muscles. The pH of muscles with zero lactate was also estimated. The BC was calculated on the basis of the amount of lactate accumulating in the muscle between two sampling times and the simultaneous pH decline. Two muscle samples were obtained from each muscle (n=13–36): one as soon as possible after slaughter and the other 24 h post-mortem. The BCs (mmol lactate/(pH kg)) were in the light gluteus superficialis, longissimus dorsi and semimembranosus muscles 48.3 ± 8.8, 48.6 ± 9.2 and 46.8 ± 13.0, and in the dark infraspinatus and masseter muscles 45.3 ± 13.1 and 32.0 ± 11.5, respectively. The dark masseter muscle differed significantly from the other muscles studied (p<0.01). The estimated pH values of muscles with zero lactate were in the gluteus, longissimus dorsi, semimembranosus muscles 7.14 ± 0.06; 7.18 ± 0.06; 7.38 ± 0.08, and in the infraspinatus and masseter muscles 6.87 ± 0.07; 7.03 ± 0.08, respectively. It was suggested since lactate is continuously formed in the muscles, the resting pH of living light and dark muscles may, however, be the same. The approach used in this study to determine the BC resulted in values which are close to values previously reported in the literature (measured by using titration curves).     

The effect of post-mortem ageing and heating on water retention in bovine muscles

The muscles semitendinosus (ST) and psoas major (PM) were removed from chilled young bull carcasses 24 h after slaughter and stored at 4 °C. At the 1st, 6th and 12th day of post-mortem ageing the chemical composition (moisture, fat, protein, collagen) and contents of free, immobilized and unfreezable water in the muscles were estimated. The muscle steaks were boiled at 100 °C, roasted at 170 °C or fried at 160 °C to an internal temperature of 75 °C, and the amounts of total, free, immobilized, and unfreezable water in heated muscles were evaluated. The unfreezable water was estimated by DSC. In the raw muscles immobilized water constituted 74–75%, free water 16.6–17.6% and unfreezable water 7–8% of the total water. Independent of time of ageing, PM muscle contained significantly more free water than ST muscle. During post-mortem ageing, changes in free, immobilized and unfreezable water in muscles were not significant. The level of free water was highest in boiled and least in fried meat, however the amount of immobilized water was highest in fried and lowest in boiled meat. The amount of unfreezable water in muscles heated after 12 days of post-mortem ageing decreased.     

Temperature Acclimation and Its Effects on Porcine Muscle Properties in Two Humidity Environments

SUMMARY— The effects of temperature and humidity on postmortem and associated muscle properties during growth of "stress susceptible" pigs were evaluated. Exposure to ambient temperatures of 32 and 21°C for alternating 3-day periods caused rapid post-mortem glycolysis, high percent light reflectance, and increased light to dark fiber ratios in the longissimus dorsi muscle as compared to constant (27°C) temperature, but only in moderate (38–42% relative) humidity environments. The above events due to temperature acclimation were masked when the humidity was low (17–23% relative). Humidity effects that were independent of temperature acclimation resulted in high percent light reflectance and high muscle temperature in the post-mortem muscle of pigs reared in low humidity. No significant differences were found in lactic dehydrogenase or succinic dehydrogenase enzyme activities of longissimus dorsi or gluteus medius muscles.     

Comparison of different magnesium sources on lamb muscle quality

Wether lambs (n=20) were used to compare the effect of dietary magnesium oxide (MgO), unweathered Magnesium Mica® (UMM) and weathered Magnesium Mica® (WMM) on muscle quality. Lambs were fed a corn-based, control diet (Ctrl), or the Ctrl supplemented with either MgO, UMM, or WMM for 95 days before harvest. Following a 24-h chill, carcasses were fabricated, and L*, a* and b* values were determined on the triceps brachii (TB), longissimus thoracis (LT), semimembranosus (SM), and semitendinosus (ST) muscles. Supplemental magnesium had no (P > 0.10) effect on live and carcass weights, fat thickness, loin eye area, and USDA yield grade. Carcasses from lambs supplemented with MgO had higher (P < 0.10) flank streaking scores and USDA quality grades than those from lambs fed diets containing UMM. Although magnesium-supplementation had no (P > 0.10) effect on marbling scores, the LT from Ctrl-fed lambs had more (P < 0.05) intramuscular lipid than lambs fed diets containing UMM or WMM. Lambs supplemented with UMM had greater (P < 0.05) LT shear force values than lambs fed MgO or WMM. Magnesium-supplementation had no (P > 0.10) effect on muscle color; however, supplementing finishing diets with UMM may result in less palatable lamb.     

Effects of halothane genotype and pre-slaughter treatment on pig meat quality. Part 1. Post mortem metabolism, meat quality indicators and sensory traits of m. Longissimus lumborum

Forty-eight castrated F₂ offspring of Piétrain and Large White pigs were allocated to a 3 × 2 factorial design in order to study the interactive effect of halothane genotype (NN, Nn and nn) and pre-slaughter treatment [referred to as ‘Experimental’ (EXP) and ‘Commercial-like’ (COL) conditions; the latter combining short transportation, mixing unfamiliar pigs and slaughtering shortly after transport] on muscle post mortem changes and meat quality. The pigs were slaughtered over 4 days. Pre-slaughter glycogen depletion in M. longissimus lumborum (LL) was greater in the nn pigs, compared with the two other genotypes. Lactate accumulation post mortem in LL muscle was greater and the pH value at 40 min post mortem was lower in nn compared with NN pigs. Nn pigs were close to nn pigs for lactate accumulation and showed intermediate pH values in the LL muscle. In the M. semimembranosus (SM), NN and Nn pigs showed the same rate of post mortem changes, as evidenced by similar glycogen, lactate, creatine phosphate and ATP levels, and pH values at 40 min post mortem. Pre-slaughter treatment did not affect the rate of post mortem changes in both muscles and no interactive effect with halothane genotype was found. The pigs slaughtered under the ‘COL’ conditions had a significantly higher ultimate pH in the LL and SM muscles than those slaughtered under the ‘EXP’ conditions. The LL muscle from nn pigs was paler (higher L*) than that of NN and Nn pigs. In SM muscle, Nn pigs showed a significantly higher L* value than NN pigs. Drip loss of the LL muscle was significantly higher in nn compared with NN pigs, the heterozygous pigs being intermediate. Sensory evaluation of the LL muscle showed that nn pigs had a lower colour intensity and colour homogeneity of raw meat than NN and Nn pigs. Tenderness was significantly lower in nn compared with NN pigs, the Nn pigs being intermediate. Pre-slaughter treatment significantly increased ultimate pH in both muscles (LL and SM) but did not affect significantly the rate of pH fall (pH₄₀). It did not affect any of the meat quality traits and no interactive effect with halothane genotype was found. These results confirmed the influence of the halothane gene on the kinetics of muscle post mortem changes and related meat quality traits. They also confirmed the intermediate position of heterozygous pigs in terms of meat quality.     

Post-natal changes in some histochemical and enzymatic characteristics of three pig muscles

Post-natal changes in histoenzymatic and enzymatic characteristics were studied in porcine longissimus, psoas major and tibialis cranialis muscles. The experiment was carried out on thirty-five female large White pigs: fifteen were slaughtered between birth and 120 kg body weight (BW) and twenty at the market liveweight of 100 kg. The relative growth of each of the three muscles in relation to BW appeared to be monophasic, the allometric growth rates differed significantly between muscles: 1·21, 1·08 and 0·97, respectively. Myofibers were classified according to their contractile and metabolic properties as βR, αR and αW fibers. In the three muscles, the percentage of β fibers increased from birth up to 2 months of age and little change occurred thereafter. Glycolytic fibers were detectable only from about one month onwards. Their percentage then increased rapidly up to 30-50 kg BW to reach 60%, 30% and 12% in longissimus, psoas major and tibialis cranialis muscles, respectively. Thereafter, these values only changed slightly. This histoenzymatic differentiation led to the typical type grouping found in pigs. Throughout the period studied, the cross-sectional area of the myofibers increased with some differences according to fiber type and muscle. Lactate dehydrogenase (LDH) specific activity was similar in the three muscles at birth and then increased rapidly up to 4-5kg BW (15-20 days of age), although to a greater extent in the future glycolytic muscle (longissimus) This increase then abruptly slowed down. The period between 15 and 20 days of age corresponds to a critical stage already known in the rearing of pigs, i.e. growth rate, body composition and food intake. Changes in isocitrate dehydrogenase (ICDH) specific activity were much slighter and differed according to the muscle. There were close correlations between percentages of αW fibers and enzymatic kinetic measurements from one month onwards.