Combination treatment of high-pressure and CaCl2 for the reduction of sodium content in chicken meat batters: effects on physicochemical properties and sensory characteristics

A combination of high-pressure processing (HPP, 200 MPa, 10 min) and CaCl₂ (0.2%, w/w) on the cooking loss (CL), expressible moisture (EM), textural properties (hardness, springiness, cohesiveness, chewiness and firmness) and sensory attributes of reduced-sodium (1.25% NaCl) chicken meat batters (RCMB) was investigated. The results revealed that combining HPP with CaCl₂ significantly decreased EM, elevated sensory properties and acted synergistically in reducing CL and improving hardness, chewiness and firmness, which enabled RCMB to obtain quality characteristics similar to those with high salt (2.5% NaCl) content. These changes brought about by combined HPP and CaCl₂ could be attributed to increased apparent viscosity and proportion of immobilised water, enhanced rheological (elastic) properties and a denser, homogeneous protein gel network with evenly dispersed small fat globules. It is interesting to take advantage of the synergy between moderate HPP and low concentrations of CaCl₂ to develop meat products with reduced sodium content.     

Effect of phosphate compounds on gel-forming ability of surimi from bigeye snapper (Priacanthus tayenus)

The properties of surimi gel from bigeye snapper (Priacanthus tayenus) added with various phosphate compounds (sodium pyrophosphate, PP; sodium tripolyphosphate, TPP; and sodium hexametaphosphate, HMP) at different levels (0%, 0.05%, 0.1%, 0.3% and 0.5% w/w) and heated under various conditions were studied. Kamaboko and directly heated gels from bigeye snapper surimi added with 0.05% PP had the increase in breaking force and deformation by 17.35% and 11.52%, and 13.54% and 3.53%, respectively, compared with the control gel (without PP addition). At the same level used (0.05%), TPP had no influence, but HMP exhibited a detrimental effect on kamaboko gel. The addition of PP (0.025%) in combination with 50 mmol CaCl₂/kg increased the breaking force by 38.68% as compared with the control gel (without additives), suggesting that the sufficient amount of CaCl₂ could enhance the setting of the gel. Generally, the marked decrease in breaking force with the coincidental increased expressible moisture was observed when the excessive amount of phosphate compounds was used (p<0.05). Microstructure study revealed that a gel with a fine network was formed with addition of PP. Therefore, the addition of PP in combination with CaCl₂ could increase the gel strength as well as water holding capacity of surimi gel.     

Effect of Concentration and Cations on Texture of Green Gram (Vigna radiata L.) Gels

The textural characteristics of gels formed with green gram (Vigna radiata L.) flour at different concentrations (8–14 g/100 g, dry solid basis) along with selected cations were studied. Gels were prepared at different concentrations of monovalent (NaCl) and divalent (CaCl₂ and FeSO₄) cations. A minimum concentration of 10 g/100 g green gram was needed to obtain a well set gel having adequate integrity, and textural (fracture strain 42–43%) and sensory characteristics (categorized as best gel by panel). The gel forming ability improved with an increase in concentrations of CaCl₂ and FeSO_4, while a high level (2 g/100 g) of NaCl was required for a well set gel having a fracture strain of about 43%. Acceptable gel with the desirable sensory attributes could be achieved with green gram at a concentration of 10 to 11 g/100 g. The presence of 0.5 g/100 g of CaCl₂ or 0.2 g/100 g of FeSO₄ could offer a gel with a green gram solid concentration of 9 g/100 g. Protein digestibility of the raw sample (55 g/100 g) improved to 89 g/100 g on gelling.     

Effects of different deodorising processes on the off‐odour compounds and gel properties of common carp surimi

In order to improve the utilisation of the common carp (Cyprinus carpio), the effects of deodorising agents [NaCl, β‐CD, malic acid (MA) and hawthorn extract (HE)] on the volatile compounds of common carp mince and its surimi gel properties were investigated. In total, thirty‐two volatile compounds were identified, seven of which were considered the main compounds of off‐odour, using HS‐SPME‐GC‐MS and OVA analyses. HS‐SPME‐GC‐MS analysis showed that MA and HE removed 84.51% and 77.18% off‐odour compounds, respectively. Three relaxation components (T₂₁, T₂₂, T₂₃) were found in surimi, and their amounts were not affected by deodorising; however, the relaxation time of major component T₂₂ was significantly increased. Surimi deodorised by HE had the highest gel strength because of its increased breaking displacement; however, a slight decrease in whiteness was observed. Thus, appropriate levels of HE could improve the palatability of surimi.     

Physicochemical properties, gel-forming ability and myoglobin content of sardine (Sardinella gibbosa) and mackerel (Rastrelliger kanagurta) surimi produced by conventional method and alkaline solubilisation process

Characteristics and gel properties of sardine and mackerel surimi produced by conventional washing process and alkaline solubilising process were investigated. The decrease in Ca²⁺-ATPase activity with the changes in the surface hydrophobicity was found in surimi produced by alkaline solubilising process (p<0.05), suggesting the denaturation of protein induced by this process. The alkal-ine solubilising process with prewashing could remove myoglobin most effectively from sardine muscle, whereas the process without prewashing resulted in the greatest myoglobin removal in mackerel muscle (p<0.05). Surimi conventionally prepared by water or NaCl washing showed the gel with greater breaking force and deformation than that from alkaline solubilising process (p<0.05). The hig-her expressible moisture was found in the gels of surimi pre-pared by alkaline process, indicating the poor water holding capacity of the gel matrix. The highest whiteness was found in the gel of sardine surimi produced by alkaline process with prewashing but the highest whiteness was obtained in the gel of mackerel surimi washed with distilled water.     

Effects of CaCl<Subscript>2</Subscript> on chemical interactions and gel properties of surimi gels from two species of carps

Effects of CaCl₂ on chemical interactions, textural properties and expressible moisture content of suwari and kamaboko gels from yellowcheek carp and grass carp were investigated. And the correlations between the contents of chemical interactions and physical properties of surimi gels were analyzed. The contents of chemical interactions, especially non-disulfide covalent bonds, disulfide bonds and hydrophobic interactions of suwari and kamaboko gels, varied with addition concentration of CaCl₂ and fish species. Suwari and kamaboko gels from yellowcheek carp exhibited higher breaking force, deformation and gel strength than these from grass carp. Surimi gels (suwari and kamaboko gels) from yellowcheek carp and grass carp exhibited their maximum gel strength when 40 mmol/kg and 100 mmol/kg CaCl₂ was added, respectively. Addition of CaCl₂ at high concentration resulted in low water holding capacity of surimi gels. Correlation analysis indicated that the contents of nonspecific associations, ionic bonds, hydrophobic interactions and sulfhydryl groups exhibited significant correlation with breaking force of surimi gels from yellowcheek carp and grass carp. Additionally, the content of non-disulfide covalent bonds had significant positive correlations with breaking force and expressible moisture of surimi gel from yellowcheek carp.     

Effect of chitooligosaccharide from squid pen on gel properties of sardine surimi gel and its stability during refrigerated storage

Effect of chitooligosaccharide from squid pen prepared using lipase (COS-L) at various concentrations (0–30 g kg⁻¹) on gel properties of sardine surimi gel was investigated. Breaking force (BF) and deformation (DF) of gel were increased, when COS-L level was increased up to 10 g kg⁻¹ (P < 0.05). Water holding capacity and whiteness of gel were improved with the addition of COS-L than those of control. Gel added with 10 g kg⁻¹ COS-L had denser network with higher likeness score for all sensory attributes, compared to control. When gel incorporated with 10 g kg⁻¹ COS-L was stored at 4 °C, BF, DF and whiteness were maintained during 10 days of storage. Textural properties of surimi gel added with COS-L were higher than those of control throughout storage. Thus, incorporation of 10 g kg⁻¹ COS-L could improve gel properties of sardine surimi gel and retarded the deterioration of gel properties during refrigerated storage.     

Effect of laver powder on textual,rheological properties and water distribution of squid (Dosidicus gigas) surimi gel

In order to ameliorate the gel quality of Dosidicus gigas surimi, the effects of laver powder on gel properties, rheological properties, and water-holding capacity (WHC) were investigated. Results indicated that the addition of laver powder could significantly increase the hardness, chewiness, and breaking force of surimi gels. However, the texture indexes and gel strength began to decline when additional amount exceeded 0.6%. Rheological results demonstrated that the addition of laver powder increased the storage modulus (G′) and viscosity of surimi, prolonged protein denaturation temperature in surimi gels. Moreover, the WHC of surimi gel was improved with the increase of laver powder. Further analyses in low-field nuclear magnetic resonance revealed that laver powder could shorten the transverse relaxation time, enhanced the combination with water, and altered the distribution of different water categories. The proportion of bound water and immobilized water reached its maximum and minimum at 0.6% of laver powder, respectively. Correlation analyses showed that WHC of surimi gel was negatively correlated well with the proportion of loose-bound water, but positively correlated with the strong-bound water and free water. In conclusion, the results supported that 0.6% was the optimal additional amount of laver powder for the squid-based surimi production based on the current ingredients of surimi products.     

Effect of Partial Substitutes of NaCl on the Cold-Set Gelation of Grass Carp Myofibrillar Protein Mediated by Microbial Transglutaminase

The objectives of this study were to investigate the changes and the relationship between structure and physiochemical properties of low sodium salt substitutes (NaCl partially replaced by KCl, CaCl₂, and MgCl₂) on grass carp myofibrillar protein gels mediated by microbial transglutaminase during cold-set gels and to provide more information about the gel characteristics. The gel strength, water holding capacity, whiteness, rheological characteristics, differential scanning calorimeter (DSC), and Raman spectra of cold-set gels were determined. The Raman spectra data were fitted to four secondary structures (α-helix, β-sheet, β-turn, and random coil). The gel properties of cold-set gels varied both with the low sodium salt types and incubation time. Myofibrillar protein (MP) gels added with NaCl and KCl had significantly higher water holding capacity than the MgCl₂, CaCl₂, and control groups. Additionally, the results showed that the gel strength and G’ value increased with the incubating time. No significant difference was detected in whiteness between the NaCl group and partial substituted groups. Cold-set gels added with the same molar amount of NaCl and KCl had fairly similar gel properties. There is a strong correlation between structural properties and gel properties of MP gels determined by DSC during the cold-set gelation process.     

The emulsifying properties of Persian gum (Amygdalus scoparia Spach) as compared with gum Arabic

The current study compares the emulsifying properties of Persian gum (PG) and gum Arabic (GA) in emulsions. The effects of concentration (0.5–3% w/v PG, 2.5–15% w/v PG), pH (2–7), ionic strength [NaCl, CaCl₂ (0–300 mM)], and temperature (40–90°C) were investigated. The surface–volume mean diameter (D₃₂) of the emulsions showed that the minimum values were 9.89 ± 0.68 and 4.52 ± 0.03 µm for emulsions containing 1.5% w/v PG and 15% w/v GA, respectively. In addition, the zeta potential of PG and GA emulsion changed from ?23.5 to ?39.5 and ?30.5 to ?46.0 mV, respectively. The interfacial tensions of PG and GA emulsions were varied in the ranges of 34.0–15.0 and 29.0–9.0 mN/m, respectively. Changes in the D₃₂ value of GA emulsions showed were not significantly different (p > 0.05) with respect to the effects of pH, NaCl, CaCl_2, and temperature. The NaCl concentration had no significant effect on D₃₂; but its value decreased from 13.11 to 5.70 µm as the CaCl₂ concentration increased. The interfacial tension of PG significantly increased with decreasing pH 7–2 and increasing 0–300 mM salts. After heating (25–90°C), the D₃₂ values of PG and GA emulsions changed to 11.04–14.54 and 4.21–4.21 μm, respectively. The results of this study can be useful for the application of PG as an emulsifier in beverage emulsions.     

Effect of NaCl on physical characteristics and qualities of chicken breast surimi prepared by acid and alkaline processing

We investigated the effect of NaCl on the physical characteristics and qualities of chicken breast surimi prepared by acid (pH 2.5 and pH 3.0) and alkaline (pH 10.5 and pH 11.0) adjustments. Salt addition resulted in increased (p < 0.05) yellowness, but decreased (p < 0.05) lightness and whiteness of surimi, regardless of pH adjustment. Surimi prepared by alkali had the highest (p < 0.001) values of lightness and whiteness. Textural properties except cohesiveness were higher (p < 0.05) in acid-prepared surimi without salt, however, addition of salt caused higher (p < 0.05) springiness, gumminess or gel strength of chicken surimi. Salt addition also caused an increase in the sensory properties. Furthermore, alkali-treated (pH 10.5 or pH 11.0) surimi had higher (p < 0.001) values of all the sensory properties. Therefore, the alkali treatment diminished gumminess and chewiness of texture property, while better color (high lightness or whiteness), gel characteristics and sensory properties were obtained from salt-added surimi. Especially the surimi at pH 10.5 and with salt was better one which had high values of whiteness, springiness and sensory properties among the surimi.     

Effect of pH and NaCl/CaCl2 on the solubility and emulsifying properties of sweet potato protein

BACKGROUND: The effects of NaCl and CaCl₂ on the solubility and emulsifying properties, namely emulsifying activity index (EAI) and emulsion stability index (ESI) of sweet potato proteins (SPPs) at pH 1–10, were investigated. RESULTS: At lower NaCl (0.1 mol L^?1) and CaCl₂ (0.05 mol L^?1) concentrations, the solubility profiles of the SPPs were very similar to those in distilled water, and the lowest solubility occurred at pH 4. Increased NaCl and CaCl₂ concentration resulted in lower SPP solubility in most of the pH studied (P < 0.05). At pH < 3, NaCl improved the EAI of SPP while at pH > 7 it reduced the EAI of the SPP (P < 0.05). Moreover, addition of NaCl also resulted in reduction of ESI of the SPP in most of the pH studied (P < 0.05). On the other hand, the presence of 0.2 mol L^?1 CaCl₂ rendered the EAI and ESI of the SPPs independent of the influence of pH. CONCLUSION: The present studies show that pH and salts modified the emulsifying properties of the SPPs, and CaCl₂ at a certain concentration could be used to improve the emulsifying properties of the protein. Copyright © 2008 Society of Chemical Industry     

Modification of volatile profiles of silver carp surimi gel by immersion treatment with hydrogen peroxide (H2O2)

The effect of immersion treatment with different concentration (0, 1.0, 5.0, 20.0 and 100.0 mm ) of hydrogen peroxide (H₂O₂) on volatile profiles and gel properties of silver carp surimi gel were investigated. The results showed that the immersion treatment with H₂O₂ obviously changed flavour characteristic of surimi gel depending on H₂O₂ concentration and different layers (outside, middle and inner). The contents of typical fishy off-flavour substances such as 1-octen-3-ol, heptanal, nonanal, octanal and decanal were largely reduced in the outside layer of surimi gel after H₂O₂ treatment, with no obvious change in the inner portion. The gel strength and whiteness were increased after H₂O₂ treatment with concentration higher than 20 mm . The content of carbonyl compounds was positively correlated with the concentration of H₂O₂, suggesting that the volatiles variation of surimi gel probably be highly related to the protein oxidation. In conclusion, immersion treatment with H₂O₂ provided a promising method to remove fishy odour of surimi gel with improved gel properties.     

Trypsin Inhibitory Activity and Gel‐Enhancing Effect of Sarcoplasmic Proteins from Common Carp

Abstract: Proteinase inhibitory activity of sarcoplasmic protein (SP) extracted from common carp (Cyprinus carpio) muscle and its gel‐improving ability were investigated. SPs displayed 89% and 54% inhibitory activity toward trypsin at 40 and 65 °C, respectively. Protein bands with molecular mass of 69, 50, 44, 41, and 35 kDa appeared on trypsin inhibitory activity staining under nonreducing condition when incubated at 40 °C, while 2 protein bands at 54 and 35 kDa were observed at 65 °C. Addition of SP at 0.18 g protein/100 g increased textural properties of threadfin bream surimi gel. However, when SP was added in combination with various CaCl₂ concentrations (0.1% to 0.5%) it did not further improve textural properties as compared to the addition of SP alone. Retention of myosin heavy chain of threadfin bream surimi was greater with the addition of SP. These results indicated that the gel‐enhancing effect of common carp SP was due to the inhibitory activity toward endogenous trypsin‐like proteinases in threadfin bream surimi. Practical Application: Sarcoplasmic protein from common carp muscle could be used as a functional protein ingredient that minimizes muscle proteolysis and improves textural properties of surimi containing trypsin‐like endogenous proteinases.     

不同品种虾糜的凝胶品质比较

本文以南美白对虾(Penaeus vannamei,PV)、斑节对虾(Penaeus monodon,PM)、罗氏沼虾(Macrobrachium rosenbergii,MR)为研究对象,通过比较不同品种虾糜的流变特性、蒸煮得率、保水性、质构特性及感官特性,研究不同品种虾糜的品质差异,以期为虾滑制品的原料选择提供理论...     

The role of salt washing of fish skins in chemical and rheological properties of gelatin extracted

Dover sole (Solea vulgaris) skins were washed with different salt solutions previous to gelatin extraction by a mild acid pre-treatment. The effects of NaCl, KCl, MgCl₂, MgSO₄ and no-salt washing on the mineral content, yield of extraction, molecular weight distribution, gel strength, aggregation phenomena and viscoelastic properties of gelatin newly dissolved and after overnight cold maturation were evaluated. Skin washing with NaCl and KCl induced noticeable changes in molecular weight distribution, and consequently in gel strength and rheological properties, especially when compared with unsalted gelatin preparations. However, salts containing Mg²⁺ gave rise to the retaining of this bivalent cation in the resultant gelatin, which is detrimental to the gelatin quality.     

Effect of oxidised tannic acid on the gel properties of mackerel (Rastrelliger kanagurta) mince and surimi prepared by different washing processes

Effect of oxidised tannic acid (OTA) at different levels (0, 0.25, 0.50 and 0.75% of protein content) on the gel properties of mackerel (Rastrelliger kanagurta) mince and surimi prepared by different washing processes was investigated. Breaking force and deformation of gels varied with washing processes and concentrations of OTA. The gel of alkaline-saline washing process surimi (ASWPS) added with 0.25% OTA had the increases in breaking force and deformation by 166.2 and 45.9%, respectively, compared with that of conventional washing process surimi (CWPS) without OTA addition. Those increases were associated with the lowered expressible moisture content. Electrophoretic studies revealed that the greater polymerisation was found in ASWPS added with 0.25% OTA. Slight retention of myosin heavy chain (MHC) with lowered trichloroacetic acid (TCA) soluble peptide contents was observed in ASWPS gel added with 0.25% OTA, suggesting the decreased degradation induced by indigenous proteases. The microstructure of ASWPS gels became more ordered, compact and denser with the addition of 0.25% OTA. The use of OTA in conjunction with alkaline-saline washing process could improve the properties of gel from mackerel surimi without adverse effect on sensory properties.     

Quality attributes and cell wall properties of strawberries (Fragaria annanassa Duch.) under calcium chloride treatment

Effects of CaCl₂ (0%, 1% and 4%) treatment on quality attributes and cell wall pectins of strawberry fruits stored at 4 °C for 15 d were investigated. Strawberry firmness was not significantly affected by CaCl₂ treatment. Compared to the other groups, the 1% CaCl₂ group had better quality attributes, including decay rate, weight loss and soluble solids content. The treatment with 4% CaCl₂ inhibited weight loss but caused phytotoxicity. During storage, the chain widths and lengths of water-soluble pectin (WSP), chelate-soluble pectin (CSP) and sodium carbonate-soluble pectin (SSP) decreased. Strawberry softening seemed to be due to modifications of CSP and SSP, especially the side chains. CaCl₂ treatment significantly slowed the breakdown of CSP and SSP chains by strengthening the ionic crosslinkages among these pectin molecules. These results illustrate the fundamental CaCl₂ effects and will help improve the application of CaCl₂ to postharvest fruits.     

Effect of furcellaran incorporation on gel properties of sardine surimi

Effects of furcellaran, powder (FURP) and solution (FURS), incorporation (2-8% based on surimi solid content) on gel properties of sardine (Sardinella albella) surimi were investigated. Breaking force, hardness, gumminess, chewiness, and whiteness of gels increased with increasing FURP or FURS contents (P < 0.05), where the highest values were observed in gel containing 8% FURS (P < 0.05). Expressible moisture content of gel decreased as levels of furcellaran increased (P < 0.05). The gel deformation, springiness, and cohesiveness were not affected by furcellaran (P > 0.05) as well as the polymerization of myosin heavy chain as visualised by SDS-PAGE. FURP and FURS could enhance the interconnection between chains during heating as indicated by the higher G′. Addition of furcellaran, especially FURS, up to 6% increased sensory property of resulting gel (P < 0.05). Anywise, an abatement in overall likeness score was found in gel with 8% FURS. Finer and denser microstructural network was observed in gel containing 6% FURS when compared to control. Therefore, furcellaran, prepared as solution, at an appropriate level can improve the overall quality of sardine surimi.     

Effects of Ca++, Mg++ and Na+ on Heat Aggregation of Whey Protein Concentrates

Effect of Ca⁺⁺ on the heat aggregation of whey protein concentrates (WPC) was compared with that of Na⁺ and Mg⁺⁺. On the alkaline side of the isoelectric zone, aggregation of WPC was increased by the addition of CaCl₂, MgCl₂ or NaCl, among which CaCl₂ showed the greatest effect. The denaturation temperature of WPC determined by differential scanning calorimetry significantly decreased in the presence of CaCl₂ or MgCl₂, but increased slightly in the presence of NaCl. In the electrophoretic patterns of heated WPC, the most sensitive protein to Ca⁺⁺ was β-lactoglobulin.