An Automated System for Processing Sample Vials Stored at Ultra-Low Temperatures

The BIOPHILE Individual Vial Retriever (IVR) system has been developed to provide automated access to vials stored at ultra-low temperatures. The IVR performs storage, retrieval, sorting, cataloging, volume estimation (weighing), barcode reading, and re-racking. All racking operations are performed in an environment designed to keep samples frozen at their optimal temperature. Operating temperatures are −80°C, −40°C, −20°C, and room temperature. Laboratory Information Management System (LIMS) integration, automation integration, chain of custody tracking, and FDA 21CFR Part 11 compliance are supported. This article introduces the IVR and provides information on its characteristics.     

Maximum isometric trunk muscle strength and activity at trunk axial rotation during sitting

This study was performed to provide information relating to the twisted posture being characteristic of the driver of an agricultural tractor working in the field. The relationship of trunk axial strength and muscle activity to trunk twisting angle of prerotation was determined and quantified. Differences between tractor drivers and office workers, and between the two directions of twisting action were also studied. Nine male tractor drivers and nine male office workers performed isometric maximum efforts at about −40, −20, 0, 20 and 40° of pre-set trunk twisting angles in both the clockwise and counterclockwise directions. Exerted torque, true angle of prerotation and muscle activity from left and right side of each of obliquus externus, rectus abdominis and erector spinae were measured simultaneously. The results showed that the subjects could exert the greatest torques when being prerotated in the opposite direction and the lowest torques when being prerotated in the same direction to the direction of exertion. The exerted torques were within the range of 65 – 145 Nm. There were large differences in obliquus externus and erector spinae activity due to the twisting direction. There were also changes in muscle activity from obliquus externus and rectus abdominis due to prerotation angle. The results raised questions concerning the involvement of the passive tissues and the use of deeper muscles during trunk axial rotation, which should be further investigated.     

Change in contact temperature of finger touching on cold surfaces

This study deals with human fingers touching cold surfaces of four materials (aluminium, steel, nylon and wood) at different surface temperatures (−20°C, −15°C, −10°C, −4°C, 0°C and 2°C). Contact finger skin-surface interface temperature and subjective responses on thermal and pain sensations were determined during touching. Type of material and their surface temperature clearly affected the contact cooling of the finger. Individual variation in finger contact cooling was significant. Contact temperature limits for human fingers touching cold surfaces are suggested according to the experimental results. In addition, time to reach a critical temperature (7°C, 5°C or 0°C) when contacting a cold metallic surface is discussed.

Relevance to industry

The outcome of this experimental study supplies as a basis for the development of an ergonomics database to determine temperature limit values for cold touchable surfaces. The critical temperatures are relevant to all industries where cold surfaces cause a risk of finger contact cooling for the manual protection in the cold operations.     

Quartz rate gyro sensor for automotive control

A new quartz rate gyro sensor has been developed for automotive controls. The principle of the sensor is detection of the Coriolis force with a vibrator. An H shape vibrator supported with a stem at its center was used. The vibrator supported at the center was good for the isolation of a small detection motion from a large excitation motion. A new mechanical coupling model and the FEM analysis were used to reduce output offset of the sensor. The detection range of the sensor is from −100 to 100°/s. The sensor output offset is small within ±1°/s in the wide temperature range from −30 to 85 °C. The developed quartz rate sensor satisfied required specifications and wide temperature stability.     

Polysilicon strain-gauge transducers

Strain-gauge transducers with polysilicon resistors can be used successfully in various sensors. They have some advantages in comparison to monocrystalline silicon strain-gauge transducers. However, not enough information is available for the qualitative design of such transducers. The theoretical principles of the temperature dependence of the output signal for a constant supply voltage and constant supply current are described. A polysilicon strain-gauge transducer with a square diaphragm fabricated on the discussed principles is reported. It has an operating temperature range of −190- +300 °C, and weak temperature dependence of the output signal.     

Fiber composite thin shells subjected to thermal buckling loads

The results of parametric studies to assess the effects of various parameters on the buckling behavior of angle-ply, laminated thin shells in a hot environment are presented in this paper. These results were obtained by using a three-dimensional finite element analysis. An angle-ply, laminated thin shell with fiber orientation of [θ/ −θ]₂ was subjected to compressive mechanical loads. The laminated thin shell has a cylindrical geometry. The laminate contained T300 graphite fibers embedded in an intermediate-modulus, high-strength (IMHS) matrix. The fiber volume fraction was 55% and the moisture content was 2%. The residual stresses induced into the laminated structure during the curing were taken into account. Parametric studies were performed to examine the effect on the critical buckling load of the following parameters: cylinder length and thickness, internal hydrostatic pressure, different ply thicknesses, different temperature profiles through the thickness of the structure, and different layup configurations and fiber volume fractions. In conjunction with these parameters the ply orientation varied from 0° to 90°. Seven ply angles were examined: 0°, 15°, 30°, 45°, 60°, 75°, and 90°. The results show that the ply angle θ and the laminate thickness had significant effects on the critical buckling load. The fiber volume fraction and the internal hydrostatic pressure had important effects on the critical buckling load. The cylinder length had a moderate influence on the buckling load. The thin shell with [θ/−θ]₂ or [θ/−θ]^s angle-ply laminate had better buckling-load performance than the thin shell with [θ]₄ off-axis laminate. The temperature profiles through the laminate thickness and various laminates with the same thickness but with the different ply thickness had insignificant effects on the buckling behavior of the thin shells.     

Maximal dynamic grip force and wrist torque: the effects of gender, exertion direction, angular velocity, and wrist angle

The objective of this study was to examine the effects of gender, exertion direction, angular velocity and wrist angle on simultaneous grip force and wrist torque under the isokinetic condition. The study used 20 participants (10 males and 10 females) and included 6 angular velocities (15, 30, 45, 60, 75, and 90°/s) and 2 wrist exertion directions (flexion and extension) over the wrist range of motion of 70° flexion to 60° extension in 5° increments. Similar to other studies, males and flexion exertion produced larger forces than females and extension exertion, respectively. However, the largest forces were generated at near extreme flexion of the wrist and the dependent variable of angular velocity was not practically significant. These results can contribute to the evaluation of cumulative trauma syndromes, but there is a need for more research on the dynamic measures of the hand and wrist complex and for standard development for dynamic force measurement.     

The importance of heel compression in the measurement of diurnal stature variation

Heel pad thickness was measured in 20 subjects (18 males aged 21–39 years and two females aged 21 and 25 years) using a 2 MHz ultrasound transducer linked to an Echo Scan body composition meter, Thandar SC110A oscilloscope and M150 amplifier. With the subjects standing erect and weight-bearing equally through both feet, the average time to achieve equilibrium heel compression was 81·7 s. Compression of the heel pad soft tissue averaged 4·4 mm (range 0·8–8·9 mm). Following equilibrium with one foot weight-bearing, an additional average loss of 1·6 mm of thickness (range 0–4 mm) was observed occurring after an average time period of 51 s.

This study has confirmed the suspicion that heel pad shrinkage may be a confounding factor in measurements of change of stature. The results of this study have indicated that a period of 2 min is needed for the heel pad thickness to compress sufficiently before measurements are taken.     

Isokinetic strength characteristics in wrist flexion and extension

A laboratory experiment was conducted to measure strength characteristics in dynamic (isokinetic) wrist flexion and extension. Twenty four college-age males exerted their maximum torque in both concentric flexion and extension at 60, 120, and 180°/s of angular velocity through a ±60° range of deviation from wrist neutral. Results show that velocity and motion direction significantly effected both peak torque as well as the postural displacement of peak torque. The value of peak torque decreased with an increase in velocity and the wrist angle at peak torque generally moved to a more deviated, flexed posture (from neutral) with increasing velocity as well. Peak torque for all velocity and motion-type conditions tested occurred in a flexed posture relative to neutral. It is anticipated that these results may be of use as biomechanically based considerations in the evaluation and design of upper extremity tasks involving wrist flexion/extension as well as to perhaps give insight into functional characteristics of the wrist. Finally, regression equations were developed to aid in the prediction of peak torque based upon task, individual and/or population parameters.

Relevance to industry

Results from this study should enhance the overall understanding of wrist functioning. Specifically, motion type, velocity of movement and wrist posture are important ergonomic design considerations. These results can also be used to modify existing biomechanical models that do not consider wrist variables.     

The BIOPHILE Sample Process Management System - Automated Sample Access at Ultra Low Temperatures

The genomics, proteomics, clinical, and drug discovery laboratories have a growing need to maintain valuable samples at ultra-low (−80°C) temperatures in a validated, secure environment. Automated sample processing systems have until now required manual (off-line) storage of samples at −80°C, reducing system reliability and speed. Both of these important needs are addressed by the Sample Process Management System being introduced by BIOPHILE Inc. Conventional sample management processes, such as storage, retrieval, and cataloging, are increasingly strained by the growing sample populations. There are variable sample types, access requirements and storage requirements. Security and inventory procedures are implemented manually. The evolving technologies present in the laboratory cannot interface with conventional manual storage techniques. Addressing these limitations, the primary benefits of BIOPHILE's solutions are:

• Fully validated sample management process that coordinates the life-cycles of samples and their related data.

• Robotic technology to securely store and retrieve samples, improving their accessibility and stability. Thermal shock is reduced, improving sample longevity and quality. The robotic technology allows integration with larger automation systems.

• A process program to develop a Sample Management Strategy. This strategy is developed by analyzing long-term research goals, current baseline processes, and identification of current sample life cycles. A full validation documentation package can be generated, providing a high level of quality assurance.

• Improved sample visibility and quality assurance - automated sample population cataloging; controlled sample management access and security.

     

Sol–gel derived polycrystalline Cr1.8Ti0.2O3 thick films for alcohols sensing application

The powder sample of Cr_1.8Ti_0.2O₃ (CTO) was obtained by a sol–gel method. The thick films were developed on identical ceramic tubes of 4 mm length comprising of two Au-electrodes and printing an eight-layer film prepared by mixing CTO with glass powder and -terpinol as an organic vehicle. X-ray powder diffraction (XRD) patterns showed the formation of a single phase. The scanning electron microscope (SEM) images of the ceramic sensor treated at 850 °C revealed that the grain size was larger than 400 nm for the individual isolated grains on the surface, and the agglomerated dense spheroidal platelets had the size of 1–4 μm in diameter. The AC impedance measurement in ambient air showed that the resistance decreased nearly by two orders of magnitude with an increase in temperature in the range of 400–600 °C for both the powder sample and the thick film, and the activation energy E_a derived from the measurement was found to be 0.35 and 0.36 eV for the powder and the film, respectively. The films were exposed to various concentrations of alcohols (0.4–1.2 ppm of methanol and 1.0–5.0 ppm of ethanol), followed by determination of sensor response, sensitivity and reversibility and reproducibility. The origin of the gas response was attributed to the surface reaction of R-OH (R = methyl and ethyl group) with O⁻_(ads) to form adsorbed R-CHO, which was desorbed as a gas at 400 °C after the sensor departing from the gas.     

Thermodynamics of the cao-si02 system

In order to maintain consistency, analytical expressions for the free energy of mixing of phases should reproduce not only the phase diagrams but also the experimentally determined activities. Information on the partial molar free energies and the phase boundaries, in turn, can be used to estimate the free energy of formation of compounds.

An examination of thermochemical data in the CaO-SiO₂ system showed that ΔGδf values for -Ca₂Si0₄, which are stable at temperatures above 1710°K, are limited a maximum of 1800°K. The free energy of formation in a temperature range from about 1700 to 2400°K was estimated from the phase boundary and the activity of silica to be as follows:

2Ca0(s) + Si0₂(cristo.) = Ca2Si0₄() ΔG°f = −86303.50 − 34.338 Tjoules

An analytical expression for the free energy of mixing of the liquid phase was obtained for the entire composition range in the CaO-Si0₂ system. Confidence in the estimated G‡f for -Ca₂Si0₄ was demonstrated by good agreement of the calculated phase diagram and the experimentally determined activity of silica.     

Humidity sensing and electrical properties of a composite material of nano-sized SiO2 and poly(2-acrylamido-2-methylpropane sulfonate)

A composite material of nano-sized SiO₂ and poly(2-acrylamido-2-methylpropane sulfonate) (poly(AMPS)) was used to make a humidity sensor. The infrared (IR) spectra and microstructure of the material were analyzed, and the humidity sensing and electrical properties of the sensor were measured. The sensor well responded to humidity with a relative good linearity, though it depended on the applied frequency. The temperature influence between 15 and 35 °C was −0.71 and −0.15% RH/°C at 30 and 90% RH, respectively. The sensor showed a negligible hysteresis and fast response time upon humidification and desiccation. The stability of the sensor in a highly humid and alcoholic environment increased with increasing the SiO₂ content. The activation energy for conduction reduced with water adsorption. The different impedance plots observed at low and high relative humidity suggested different sensing mechanisms of the SiO₂/poly(AMPS) composite material.     

High-resolution high-voltage sensor based on SAW

A surface acoustic wave (SAW)-based high-voltage sensor is described. The sensor consists of a SAW oscillator fabricated on a 10 mm × 10 mm 128° rotated Y-cut, X-propagating LiNbO₃ substrate. The voltage is applied to electrodes on the substrate, and the resulting electric field changes the propagation time of the SAW. The propagation time is directly related to the output frequency of the SAW oscillator. The high-voltage sensor offers a small-sized high-voltage measurement device with several attractive features: a high resolution (better than 0.2 V up to 2.4 kV, better than 0.4 V for higher voltages), a large range (−10 to +10 kV), a high input impedance (> 10¹³ ω) and a low input capacitance (< 10 pF). The sensitivity amounts to 16 Hz V⁻¹.     

Comparison between required clothing insulation and that actually worn by workers exposed to artificial cold

Required Clothing Insulation (IREQ) is a new thermal index submitted to the International Organisation for Standardisation (ISO) for discussion. It is designed to prevent general body cooling and is based on an analysis of heat exchanges. The thermal clothing insulation actually worn (lcl) is estimated using a new method, also submitted to ISO.

IREQ of 54 workers exposed to artificial cold (air temperature between −30° C and +10° C) was compared with lcl actually worn by these workers. The results of the present study show that, on average, the workers choose accurately lcl they need if their IREQ is below and up to 1·5 clo. Moreover, these workers prefer to wear garments which provide them with thermal comfort. If IREQ of workers is higher than 1·5–2 clo (i e, workers exposed to −20° C), it is difficult for them to increase their thermal insulation with additional garments. Although their lcl is not sufficient, there is no risk of gradual body cooling because of their continuous time exposure (CTE) which is shorter than the calculated Duration Limited Exposure (DLE). On the other hand, Wind Chill Index (WCI), which is proposed to prevent local cooling, is better adapted to prevent cold injuries than physiological thermal strain; for example, impairment of manual dexterity cannot be prevented with this index.     

Silicon pressure sensor with integrated bias stabilization and temperature compensation

A piezoresistive pressure sensor for relative or differential pressure applications has been developed. The use of a 10 μm thick bossed membrane provides high sensitivity (typically 3.3 μV/V Pa) in the low-pressure range (0–60 hPa). Adaptation of sensor fabrication to a triple-diffusion bipolar process by application of an electrochemical etch-stop at a diffused n-type layer on a p-substrate allows monolithic integration of two different conditioning circuits operating with battery voltages of 12 to 20 V. Both circuits provide bias stabilization and temperature compensation for temperatures between −30 and +90°C. Thus the possibility of sensor fabrication by using standard IC technologies is demonstrated.     

The assessment of heat radiation

Approximately 900 climatic chamber experiments were performed with 16 male subjects to study the thermal strain at climates including increased heat radiation. Based on the reactions of heart rate, rectal temperature and sweat rate, a heat stress index was developed for the assessment of climates with effective heat radiation intensities up to 1400 W m⁻². The index considers different combinations of dry air temperature (5–55°C), globe temperature (25–76°C), mean radiant temperature (25–160°C), air velocity (0.5–2.0 m s⁻¹), clothing, physical work load and directions of radiation and air flow.

The index integrates combinations of the variables producing the same degree of thermal strain into a single value. This value indicates the temperature of the physiologically equivalent climate in which air and radiant temperature are equal. It can be determined from a simple formula or from correspondent graphs.

In comparison, the international recommended heat stress indices are less capable to evaluate heat radiation correctly. The incorporation of the new partial index into the used indices may improve substantially their physiological validity in the assessment of climates with radiant heat stress.

Relevance to industry

The goal of this paper is to provide an improved assessment of thermal stress in working environments in which heat radiation is an important heat stress factor.     

Optimal estimation of sea surface temperature from split-window observations

Optimal estimation (OE) improves sea surface temperature (SST) estimated from satellite infrared imagery in the “split-window”, in comparison to SST retrieved using the usual multi-channel (MCSST) or non-linear (NLSST) estimators. This is demonstrated using three months of observations of the Advanced Very High Resolution Radiometer (AVHRR) on the first Meteorological Operational satellite (Metop-A), matched in time and space to drifter SSTs collected on the global telecommunications system. There are 32,175 matches. The prior for the OE is forecast atmospheric fields from the Météo-France global numerical weather prediction system (ARPEGE), the forward model is RTTOV8.7, and a reduced state vector comprising SST and total column water vapour (TCWV) is used. Operational NLSST coefficients give mean and standard deviation (SD) of the difference between satellite and drifter SSTs of 0.00 and 0.72 K. The “best possible” NLSST and MCSST coefficients, empirically regressed on the data themselves, give zero mean difference and SDs of 0.66 K and 0.73 K respectively. Significant contributions to the global SD arise from regional systematic errors (biases) of several tenths of kelvin in the NLSST. With no bias corrections to either prior fields or forward model, the SSTs retrieved by OE minus drifter SSTs have mean and SD of − 0.16 and 0.49 K respectively. The reduction in SD below the “best possible” regression results shows that OE deals with structural limitations of the NLSST and MCSST algorithms. Using simple empirical bias corrections to improve the OE, retrieved minus drifter SSTs are obtained with mean and SD of − 0.06 and 0.44 K respectively. Regional biases are greatly reduced, such that the absolute bias is less than 0.1 K in 61% of 10°-latitude by 30°-longitude cells. OE also allows a statistic of the agreement between modelled and measured brightness temperatures to be calculated. We show that this measure is more efficient than the current system of confidence levels at identifying reliable retrievals, and that the best 75% of satellite SSTs by this measure have negligible bias and retrieval error of order 0.25 K.     

A 400°C silicon Hall sensor

The maximum operating temperature of conventional silicon sensors is limited to about 200°C, due to excessive thermal generation of carriers at higher temperatures. The minority-carrier exclusion effect can be exploited to reduce the number of thermally generated carriers, ultimately maintaining extrinsic carrier concentrations at intrinsic temperatures. Based on this effect, a silicon magnetic-field sensor with a maximum operating temperature of about 400°C is presented. The sensitivity has been improved by about 500% with respect to a previously reported version, and now measures about 60 V (A T)⁻¹ at room temperature. Additionally, the theoretical support of the exclusion effect has been improved with a more accurate analytical model.     

Strain–temperature discrimination using a step spectrum profile fibre Bragg grating arrangement

A sensing head for simultaneous measurement of temperature and strain is presented and analyzed. The proposed configuration is based on the combination of two Bragg gratings, written in different fibres and with different reflectivities, to form a single signature with a reflected step spectrum profile. This characteristic minimizes the spectrum allocated to each sensor in a series multiplexing topology. By measuring the changes in the peak wavelength and spectral width of this signature, resolutions of ±0.65 °C/√Hz and ±2.55 μ/√Hz were achieved for temperature and strain measurements, respectively.