Effect of modified atmosphere packaging on quality factors and shelf-life of surface mould ripened cheese: Part I constant temperature

In the present study, packaging of a surface mould ripened cheese under 2 atm: MAP-A (0% O₂, 27 ± 6% CO₂) and MAP-B (2 ± 1% O₂, 19 ± 2% CO₂) was studied at 12 °C and the results were compared with the existing commercial packaging system (wrapped with waxed paper and inserted in cardboard box). Quality parameters such as colour, texture, pH and moisture content were evaluated after 0, 7 and 14 days of storage, together with a sensory evaluation. Tuckey test and principal components analysis showed that after 14 days of storage, the best conditions for the preservation of the cheeses corresponded to MAP-B. The predicted shelf-life was found to be 14, 6 and 17 days for control, MAP-A and MAP-B respectively. It was concluded that modified atmosphere packaging of surface mould ripened cheese with low levels of O₂ (1-3%) and relatively high levels of CO₂ (17-21%) can be used to extend the shelf-life of soft cheese; however the package has to be suitably designed, as total loss of O₂ (as in MAP-A) would shorten the shelf-life.     

Effect of modified atmosphere and active packaging on the shelf-life of fresh bluefin tuna fillets

The aim of this work was to study the influence of the combined use of MAP and antioxidant-based active packaging on the shelf-life of fresh bluefin tuna fillets stored at 3 °C. Active packaging films were produced by embedding α-tocopherol into an unstabilized low density polyethylene (LDPE) matrix at three concentrations (0.1%, 0.5%, 1%). α-Tocopherol release kinetics, in vitro antioxidant activity, oxygen permeability and crystallinity degree were determined to characterize the film. Preliminary shelf-life tests were performed to select critical quality indices, the best gas composition and the best α-tocopherol concentrations in the active film. Then, the effectiveness of the chosen active packaging film in combination with MAP was assessed by monitoring critical quality indices of fresh bluefin tuna fillet during storage at 3 °C for 18 days. Obtained results showed that (i) 100% N₂ atmosphere has a protective effect on haemoglobin and lipid oxidation processes monitored, (ii) active film is able to reduce fat oxidation, (iii) the combined effect of MAP and active packaging can be considered a valuable tool to increase the shelf-life of raw fish products.     

Quality and shelf-life of washed fresh-cut asparagus in modified atmosphere packaging

Fresh-cut asparagus is one of the most popular fresh vegetables for healthy consumption. However, the level of microbial load in the raw vegetable can cause food poisoning and shorten its shelf-life of asparagus. The objectives of this work were to determine the effect of chlorinated and ozonated water in the washing process to reduce the microbial load on fresh green asparagus and the effect of modified atmosphere packaging (MAP) on asparagus quality. Washing at 10 °C for 15 min with chlorinated water (100 mg/L free Cl₂) reduced aerobic plate count which had higher efficiency on microbial reduction than the use of ozonated water (0.1 mg/L O₃). No significant differences on the amount of Escherichia coli contamination among washing methods were found. Asparagus in modified atmosphere packaging retarded the deterioration process. Changes in hue angle followed a first-order kinetic reaction. Temperature dependence of the kinetic rate constant during storage time of asparagus obeyed the Arrhenius relationship with an activation energy (E_a) 29.33 ± 4.60 kJ/mol. The shelf-life prediction equation was related well with real practice. The combination of appropriate washing process and MAP increased the food safety, maintained the quality and prolonged the shelf-life of asparagus.     

Development of shelf-life kinetic model for modified atmosphere packaging of fresh sliced mushrooms

Mushrooms are highly perishable and their shelf-life depends on processing, package properties and environmental conditions during storage and distribution. The aims of this work were to evaluate the effect of temperature and number of film perforations on quality and develop shelf-life kinetic model for a modified atmosphere packaging (MAP) for sliced button mushrooms. Sliced mushrooms were packed in a tray, covered with cellophane film, and stored for 7 days at four levels of temperature (0, 5, 10, and 15 °C) and three levels of perforations at each temperature ranging the number of perforations from 1 (58 perforations per m²) to 6 (349 perforations per m²). Headspace gas composition and quality parameters (weight loss, pH, firmness and colour) were measured throughout the storage period. Increasing the storage temperature required an increase of the number of perforations in order to obtain the optimum MAP conditions. Temperature had a significant effect (p < 0.05) on quality of sliced mushrooms. Firmness was identified as a critical quality parameter; therefore, a kinetic model was developed to describe the influence of temperature on firmness and predict shelf-life of sliced mushrooms. Fresh sliced mushrooms had a shelf-life of 1, 2, 4, and 7.5 days at 15, 10, 5, and 0 °C, respectively, under optimum MAP conditions.     

Extending the shelf-life and proximate composition stability of ready to eat foods in vacuum or modified atmosphere packaging

The stability of the proximate composition (moisture, proteins, lipids and ash) and the microbiological state of cooked ‘ready to eat’ foods in vacuum or modified atmosphere packaging were examined and compared with conventionally packaged (in air) foods. The study was carried out for 7 and 29 days, during which time the food products were stored at 3°C. Vacuum or modified atmosphere packaging were effective for prolonging the shelf-life of the studied products up to 29 days with minimal changes in the proximate composition. Aerobic mesophilic, psychrotrophic microrganisms, and yeasts and moulds increased with time regardless of packaging type but more rapidly under conventional packaging.     

Effect of modified atmosphere packaging and storage conditions on photooxidation of sliced Havarti cheese

European Food Research and Technology - The impact of different modified atmosphere packaging and storage conditions on photooxidation of sliced Havarti cheese during 168&;nbsp;h storage has...     

Use of galactomannan edible coating application and storage temperature for prolonging shelf-life of “Regional” cheese

The objectives of this work were to determine the influence of the application of two different coatings (galactomannan and chitosan) and of storage temperature on the gas exchange rate of “Regional” cheese; subsequently, the coating that showed the greatest influence on the cheese gas exchange and simultaneously decreased the O₂ consumption (_RO₂)$(R_{{\text{O}}_2})$ and the CO₂ production (_RCO₂)$(R_{{\text{CO}}_2})$ rates was applied on cheese, being the shelf-life parameters monitorized through the performance of chemical and microbiological analyses. Both coatings caused a reduction of _RO₂$R_{{\text{O}}_2}$ and _RCO₂$R_{{\text{CO}}_2}$ of the cheese (between 0.19- and 1.30-fold for _RO₂$R_{{\text{O}}_2}$ and between 0.19- and 1.50-fold for _RCO₂$R_{{\text{CO}}_2}$, depending on the temperatures). The cheese coated with the galactomannan coating was the one with the lower values of _RO₂$R_{{\text{O}}_2}$ (between 0.195 and 0.635 mL kg⁻¹ h⁻¹) and _RCO₂$R_{{\text{CO}}_2}$ (between 0.125 and 0.900 mL kg⁻¹ h⁻¹). Temperature was also found to have an important effect on _RO₂$R_{{\text{O}}_2}$ and _RCO₂$R_{{\text{CO}}_2}$, its influence being well described by an Arrhenius equation with coefficients of determination, R², of 0.85 and above. The chemical and microbiological analyses showed that the application of the coating in cheese samples can be used to decrease the water loss and the colour changes during the storage time. The presence of the coating decreased the moisture loss of the cheese in 2.5% and 1.9%, and the weight loss in 3.8% and 3.1% at 4 °C and 20 °C, respectively. Also, the hardness of the cheese can be decreased as a result of the interaction of the presence of the coating with changes in the storing temperature. In the studied range (4–20 °C) temperature has a statistically significant effect in moisture loss, colour change, hardness and total mesophilic bacterial growth.     

Combined effect of irradiation and modified atmosphere packaging on shelf-life extension of chicken breast meat: microbiological, chemical and sensory changes

In the present study the combined effect of gamma irradiation (2 and 4 kGy) and modified atmosphere packaging (MAP) (30% CO₂/70% N₂ and 70% CO₂/30% N₂) on shelf life extension of fresh chicken meat stored under refrigeration was investigated. The study was based on microbiological (TVC, Pseudomonas spp., Lactic Acid Bacteria, Yeasts, Brochothrix thermosphacta, Enterobacteriaceae), physicochemical (pH, TBA, color) and sensory (odor, taste) changes occurring in chicken samples. Microbial populations were reduced by 1–5 log cfu/g for a given sampling day depending on the specific treatment. The effect was more pronounced in the case of the combination of MAP (70% CO₂/30% N₂) and the higher irradiation dose of 4 kGy. Of the chemical indicators of spoilage, TBA values for all treatments remained lower than 1 mg malondialdehyde (MDA)/kg meat throughout the 25 day storage period. pH values varied between 6.4 (day 0) and 5.9 (day 25). The values of the color parameters L*, a* and b* were not considerably affected by MAP. Irradiation resulted in a small increase of the parameter a*. Irradiation had a greater effect in extending the shelf life of chicken as compared to MAP. Sensory evaluation showed that the combination of irradiation at 4 kGy and MAP (70% CO₂/30% N₂) resulted in the highest shelf-life extension by 12 days compared to the air packaged samples.     

Effect of grafting and modified atmosphere packaging on eggplant quality parameters during storage

The physico‐chemical parameters (pH, mechanical firmness and vitamin C) and sensory parameters of grafted and ungrafted eggplant plants were studied in relation to storage time (up to 17 days at 10 °C). Eggplant plants of cultivar ‘Tsakoniki’ were grafted on Solanum torvum and S. sisymbriifolium rootstocks in order to avoid the soil borne disease caused by Verticillium dahliae. The fruits were stored under modified atmosphere packaging (MAP). Vitamin C was negatively affected by grafting it storage, while MAP prolonged the shelf life. Although pH was not affected by grafting but was positively affected by MAP. Flesh firmness was negatively affected by grafting and reduced over storage, but positively affected by MAP. Sensory analysis showed higher ratings of fruits from ungrafted plants for sweetness, acceptance and hardness whereas no difference was detected for overall acceptance. Fruits stored under MAP were better maintained compared with those stored in air.     

Effects of packaging atmospheres on shelf-life quality of ground ostrich meat

Fresh ground ostrich meat was packaged under high oxygen (O2), high nitrogen (N2), vacuum (VAC) and ambient air (AIR) atmospheres, stored at 4 ± 1 °C and displayed under 1700 ± 100 lux of fluorescent lighting for 9 days. The meat was evaluated for changes in typical shelf-life characteristics consisting of pH, color properties (CIE L^∗, a^∗, b^∗, and total color difference, ΔE), oxidative changes (thiobarbituric acid value and hexanal content) and bacterial counts (total viable cell, coliform, lactic acid bacteria, Enterobacteriaceae, Pseudomonas spp.) Initial meat pH was 6.16 and declined slightly during storage. TBA values and hexanal content were highest in O2 and lowest (P ? 0.05) in VAC and N2 atmospheres. Surface lightness (L^∗) and redness (a^∗) were highest in O2 packaging initially, decreasing (P ? 0.05) by day 9. ΔE of the ground ostrich increased during storage in only O₂ and AIR packaging. All packaging methods had generally similar effects on microbial outgrowth. Total aerobic bacteria attained >10⁶ CFU/g meat between day 3 and day 6. Ground ostrich meat was below saleable quality in less than 6 days based on all of the meat attributes. For O2 packaging however, quality based on lipid oxidation and color properties indicated a shelf-life of less than 3 days. Oxidation is likely the limiting factor for shelf-life of ground ostrich meat.     

气调包装对太平洋牡蛎冷藏保鲜效果的研究

研究了气调包装(60%CO2/40%N2、60%CO2/30%N2/10% O2)对太平洋牡蛎的气调保鲜效果.以空气包装组为对照,通过感官评定、pH、TVB-N、需氧菌数几项指标来评价气调包装的牡蛎样品在冷藏务件下(4±1℃)的贮藏效果,并对对照组和气调包装组的腐败样品进行了细菌菌相分析.实验结果表明,气调包装有效延长了牡蛎的冷藏货架期,对照组、CO2/N2组和CO2/N2/O2组的赁架期分别为6～7d、9～10d和11～12d.货架期终点时,对照组牡蛎样品以革兰氏阴性菌为主,其中假单胞菌(Pseudomonas)比例高达55%,是常规包装牡蛎冷藏的特定腐败菌;气调包装组样品以革兰氏阳性菌为主,其中CO2/N2组的优势菌为乳酸菌(Lactic acid bacteria),CO2/N2/O2组的优势菌为棒状杆菌(Corynebacterium).     

Effects of bactericides and modified atmosphere packaging on shelf-life of Chinese shrimp (Fenneropenaeus chinensis)

Effects of different bactericides and modified atmosphere packaging (MAP) on aerobic plate counts (APCs), total volatile base-nitrogen (TVB-N) and organoleptic evaluation of overall acceptable score (OA score) of Chinese shrimp (Fenneropenaeus chinensis) during cold storage were investigated. Results indicated that APC in MAP(40%CO₂/30%O₂/30%N₂) shrimp treated with compound bactericide reached 10⁷ cfu/g on the 13th day of storage, while that of ozonated water or water control treatments exceed 10⁷ cfu/g on the 9th day. APC in shrimps treated with MAP (40%CO₂/30%O₂/30%N₂) or 100%CO₂ after soaking with compound bactericide reached close to 10⁷ cfu/g at day 13, while that of air treatment exceed 10⁷ cfu/g. TVB-N value in MAP(40%CO₂/30%O₂/30%N₂) shrimp treated with compound bactericide was slightly higher than the upper threshold of 30 mg/100 g on the 17th day, while that of ozonated water treatment or water control increased to or over the threshold value on the 9th day. TVB-N value in shrimps treated with MAP (40%CO₂/30%O₂/30%N₂) or 100%CO₂ after soaking with compound bactericide were significantly lower than that of air control on the 17th day (P ≤ 0.01), with a value of 33.6 mg/100 g and 42-47.6 mg/100 g respectively, compared to 78.4-86.8 mg/100 g in air control. The lowest OA score of MAP(40%CO₂/30%O₂/30%N₂) whole and decapitated shrimps treated with compound bactericide appeared on the 17th and 21th day, respectively, compared with the 9th day in whole shrimp and the 13th day in decapitated shrimps treated with ozonated water and water control. The lowest OA score of whole and decapitated shrimps treated with MAP (40%CO₂/30%O₂/30%N₂) or 100%CO₂ after soaking with compound bactericide appeared on the 17th and 21th day, respectively, compared with the 13th and 17th day in air control. In conclusion, when combined the parameters determined together, the shelf-life of Chinese shrimp at 2 ± 1 °C, either whole or decapitated, treated with MAP (40%CO₂/30%O₂/30%N₂) and 100%CO₂ after soaking with compound bactericide were 13 and 17 days, respectively.     

包装对低温贮藏盐水鸡产品货架期及优势污染菌群的影响

盐水鸡是一种具有客家风味的传统鸡肉制品。为寻找一种能经济、有效地延长冷链销售盐水鸡货架期的包装方式,本文比较了普通包装、真空包装和气调包装对盐水鸡产品理化指标和优势污染菌群的影响。研究结果表明,盐水鸡在低温贮藏期间,各种包装对盐水鸡的含水量、pH和挥发性盐基态氮的影响不显著(p>0.01);在低温贮藏期间,各种包装中好氧性菌落总数呈线性增长,其增长速率为普通包装>气调包装>真空包装。在整个短期低温贮藏期间,没有在各种包装的盐水鸡样品中检测出产气芽孢梭菌,大肠菌群及乳酸菌,但能检测出少量的霉菌和酵母。采用16srDNA序列分析法对各种包装中污染的优势好氧性菌落进行了分类鉴定。鉴定结果表明,各种包装中的优势好氧性菌有Pseudomonas sp., Staphyloccocus aureus,Exiguobacterium sp.,和Carnobacterium sp.,且各种包装中优势菌群存在明显差异。     

Effect of modified atmosphere and vacuum packaging on some quality characteristics and the shelf-life of “morcilla”, a typical cooked blood sausage

The effect of modified atmosphere and vacuum packaging on the shelf-life of “morcilla”, a traditional cooked blood sausage, was investigated. A total of 99 “morcillas” were packaged under vacuum and in modified atmosphere using three different gas mixtures: 15:35:50/O₂:N₂:CO₂ (atmosphere 1), 60:40/N₂:CO₂ (atmosphere 2) and 40:60/N₂:CO₂ (atmosphere 3), and stored during 2, 4, 6 and 8 weeks at 4 °C. Shelf life evaluation was based on pH, water activity (a_w), colour (CIE L*, a*, b*, C* and h*), TBARS formation and microbial counts. The results indicated that, in general, storage time affected (P < 0.05) all parameters whereas no significant differences were observed (P > 0.05) among packaging conditions. Based on the microbial counts, the shelf-life of “morcilla” would be greater than 8 weeks for all packaging conditions. Samples packaged with high CO₂ concentrations (40:60/N₂:CO₂) showed the lowest values of TBARS at the end of storage.     

自发气调包装对辣椒贮藏品质的影响

为探究不同自发气调包装对辣椒贮藏品质的影响,以\     

Effect of temperature and modified atmosphere packaging on overall quality of fresh-cut bell peppers

The effect of vacuum packaging (VP) and modified atmosphere packaging without vacuum (MAP) on shelf-life of fresh-cut green bell pepper stored at 5°C and 10°C were evaluated. In-package atmosphere, overall quality, percentage of leaked juice, texture, ascorbic acid content, ethanol and acetaldehyde, and microbial growth, were evaluated at different intervals of storage. MAP-fresh-cut peppers presented better visual quality, less leaked juice and higher firmness than those stored under VP. Microbiological and quality analysis revealed a limit of shelf-life of 14 and 21 days, when fresh-cut peppers were stored at 10°C and 5°C, respectively. We conclude that MAP could be used to maintain quality attributes of fresh-cut peppers for up 21 days at 5°C.     

Combined effect of oregano essential oil and modified atmosphere packaging on shelf-life extension of fresh chicken breast meat, stored at 4 degrees C

The combined effect of oregano essential oil (0.1% and 1% w/w) and modified atmosphere packaging (MAP) (30% CO2/70% N2 and 70% CO2/30% N2) on shelf-life extension of fresh chicken meat stored at 4 degrees C was investigated. The parameters that were monitored were: microbiological (TVC, Pseudomonas spp., lactic acid bacteria (LAB), yeasts, Brochothrix thermosphacta and Enterobacteriaceae), physico-chemical (pH, TBA, color) and sensory (odor and taste) attributes. Microbial populations were reduced by 1-5 log cfu/g for a given sampling day, with the more pronounced effect being achieved by the combination of MAP and oregano essential oil. TBA values for all treatments remained lower than 1 mg malondialdehyde (MDA) kg(-1) throughout the 25-day storage period. pH values varied between 6.4 (day 0) and 5.9 (day 25). The values of the color parameters L*, a* and b* were not considerably affected by oregano oil or by MAP. Finally, sensory analysis showed that oregano oil at a concentration of 1% imparted a very strong taste to the product for which reason these lots of samples were not scored. On the basis of sensory evaluation a shelf-life extension of breast chicken meat by ca. 3-4 days for samples containing 0.1% oregano oil, 2-3 days for samples under MAP and 5-6 days for samples under MAP containing 0.1% of oregano oil was attained. Thus oregano oil and MAP exhibited an additive preservation effect.     

Effect of ripeness on the shelf-life of fresh-cut melon preserved by modified atmosphere packaging

Fresh-cut ‘Piel de Sapo’ melon was processed at different ripeness stages and stored under modified atmosphere packaging for 35 days at 5 °C. Raw material firmness and soluble solids content ranged from 6.5 to 3.9 N and 11.1 to 14.3 °Brix, respectively. The effects of a 2.5 kPa O₂+7 kPa CO₂ packaging atmosphere and a dip of 1% ascorbic acid and 0.5% calcium chloride on physiology, microbiological stability as well as color and firmness were evaluated. An intermediate stage of ripeness at processing was the most suitable to extend the shelf-life of fresh-cut ‘Piel de Sapo’ melon. Green-mature fresh-cut melon reduced CO₂ accumulation and ethanol production. In addition, a treatment with ascorbic acid and calcium chloride in combination with modified atmosphere packaging, contributed to a greater extension of the shelf-life of fresh-cut melon than that reported for fruits stored under non-modified atmosphere, slowing down the growth of microbial populations, maintaining the original color and reducing softness. Thus, the shelf-life of green-mature fresh-cut ‘Piel de Sapo’ melon dipped in an ascorbic acid and calcium chloride solution and packaged under modified atmosphere was about 10 days.     

气调储藏中不同平衡气体对鸡蛋保鲜品质的影响

采用高浓度的CO2,以不同浓度的N2、O2、空气作为平衡气体储藏鸡蛋,以新鲜度指标中的失重率、蛋黄指数、哈夫单位、蛋白pH为指标,研究了气调包装对鸡蛋保鲜效果的影响。本实验结果表明:鸡蛋采用体积分数60%~100%CO2的气调包装,在25℃条件下贮藏,质量损失率、蛋白pH保持相对较低水平,蛋黄指数、哈夫单位保持相对较高水平。贮藏28d后,不包装的对照组已经散黄,空气包装组鸡蛋降到了B级,而气调包装组鸡蛋仍保持AA级左右,各气调实验组保鲜效果差异不显著。      

Shelf-life of Domiati cheese under modified atmosphere packaging

The present study describes the effects of modified atmosphere packaging (MAP) on shelf-life extension, chemical, microbiological, and sensory properties of Domiati cheese. Five different MAP were studied [10% CO₂/90% N₂ (G1), 15% CO₂/85% N₂ (G2), 25% CO₂/75% N₂ (G3), 100% CO₂ (G4), and 100% N₂ (G5)]. Control samples were packaged in air (CA) and under vacuum. In both groups of cheeses, chemical analysis was significantly affected by MAP during cold storage. Ripening indexes were significantly affected by MAP during cold storage. Microbiological data showed that G4, followed by G5, were the most effective groups inhibiting the growth of total aerobic mesophilic and psychrotrophic bacteria, and yeasts and molds until the end of storage. Sensory evaluation was significantly affected by MAP and storage period, at 45 d CA cheese samples were judged as unacceptable. The best sensory properties were obtained in G5, G4, and G3 treatments, and recorded a relatively higher sensory evaluation scores. The best shelf-life extension was obtained in G5, G4, and G3 treatments.