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1,8-萘酰亚胺类化合物的荧光光谱性能研究 总被引:2,自引:0,他引:2
研究了11种新型1,8-萘二甲酰亚胺类荧光化合物的荧光光谱性能。利用紫外光谱仪和荧光光谱仪测定了这两类化合物的紫外光谱和荧光光谱,分别得到最大吸收波长、最大激发波长、最大荧光发射波长,并以硫酸奎宁的0.5mol/l硫酸水溶液为参比标准,测定了各化合物的荧光量子产率在此基础上,研究了浓度、溶荆对荧光性能的影响、以及化合物结构与荧光性能的关系。结果表明,1,8-萘酰亚胺粪化合物随着浓度的增大,荧光光谱发生红移,且斯托克斯位移增大。随着溶剂极性的增大,最大荧光发射波长发生红移,斯托克斯位移增大,荧光量子产率增大在1,8-萘酰亚胺类化合物的4-位引入笨并呋喃取代基后,最大荧光发射波长红移70nm~100nm,斯托克斯位移增大20nm~50nm,荧光量子产率明显增大。 相似文献
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设计合成了8-羟基喹啉衍生物5以及它的金属锌配合物6,经质谱(MS)、元素分析(EA)、红外光谱(IR)、紫外光谱(UV)、核磁共振氢谱(1HNMR)进行了表征,同时测定了化合物6在365 nm处的发光图和热重分析。结果表明,化合物6在365 nm的光照射下,能够发出橙红色的光,同时具有较高的热稳定性。 相似文献
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合成了对氧极为敏感的荧光试剂Ru(phen)3^2 ,通过光学显微镜,紫外-可见分光光度计,荧光分光光度计,质谱仪等仪器分析了试剂的晶体结构,紫外-可见吸收峰和荧光光谱等特性,以醋酸纤维素为载体制备了Ru(phen)3^2 荧光试剂薄膜,测试了氧气对试剂的猝灭性,为胆固醇生物传感器的研究奠定了基础。 相似文献
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用5—溴水杨醛设计合成了5—溴,N(2—磺酸基乙基)水杨醛亚胺Schiff碱,其组成和结构己由元素分析和红外光谱所表征。 相似文献
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Jasmine N. Tutol Hiu C. Kam Prof. Dr. Sheel C. Dodani 《Chembiochem : a European journal of chemical biology》2019,20(14):1759-1765
Chloride-sensitive fluorescent proteins generated from laboratory evolution have a characteristic tyrosine residue that interacts with a chloride ion and π-stacks with the chromophore. However, the engineered yellow-green fluorescent protein mNeonGreen lacks this interaction but still binds chloride, as seen in a recently reported crystal structure. Based on its unique coordination sphere, we were curious if chloride could influence the optical properties of mNeonGreen. Here, we present the structure-guided identification and spectroscopic characterization of mNeonGreen as a turn-on fluorescent protein sensor for chloride. Our results show that chloride binding lowers the chromophore pKa and shifts the equilibrium away from the weakly fluorescent phenol form to the highly fluorescent phenolate form, resulting in a pH-dependent, turn-on fluorescence response. Moreover, through mutagenesis, we link this sensing mechanism to a non-coordinating residue in the chloride binding pocket. This discovery sets the stage to further engineer mNeonGreen as a new fluorescent protein-based tool for imaging cellular chloride. 相似文献
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Xiangyu Guan Defeng Li Jiangwei Song Yanyan Ji Feng-Shou Xiao 《Journal of Porous Materials》2008,15(5):527-533
A fluorescent mesoporous silica solid (F-MCM-41) has been successfully prepared by one-pot hydrothermal synthesis from TEOS
and 2-((3-(triethoxysily)propylimino)methyl)phenol in the presence of CTMABr surfactant, followed by removal of CTMABr in
F-MCM-41 from extraction of ethanol solution at 50 °C for 5 h. The fluorescent sample was characterized with X-ray diffraction,
nitrogen isotherms, nuclear magnetic resonance (NMR), and fluorescence spectroscopy, and the obtained results show that the
sample has ordered hexagonal mesoporous symmetry, uniform pore size, high BET surface area, large pore volume as well as good
fluorescent properties. When this sample is used to detect Be2+ ions in water, the degree of fluorescence quenching is dependent on the concentration of Be2+ solutions ranged from 1 to 20 μg/L. 相似文献
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Vladimir Burilov Egor Makarov Diana Mironova Elza Sultanova Islamiya Bilyukova Kevser Akyol Vladimir Evtugyn Daut Islamov Konstantin Usachev Timur Mukhametzyanov Svetlana Solovieva Igor Antipin 《International journal of molecular sciences》2022,23(23)
Artificial gene delivery systems are in great demand from both scientific and practical biomedical points of view. In this paper, we present the synthesis of a new click chemistry calix[4]arene precursor with free lower rim and new water-soluble calixarene triazoles with 12 amino-groups on the upper rim (one with free phenol hydroxyl groups and two another containing four butyl or tetradecyl fragments). Aggregation in the series of amino-triazole calixarenes of different lipophilicity (calixarene with free phenol hydroxyl groups or butyl and tetradecyl fragments on the lower rim) was studied using dynamic light scattering and fluorescent pyrene probe. It was found that calix[4]arene with a free lower rim, like alkyl-substituted butyl calix[4]arene, forms stable submicron aggregates 150–200 nm in size, while the more lipophilic tetradecyl –substituted calix[4]arene forms micellar aggregates19 nm in size. Using UV-Vis spectroscopy, fluorimetry and CD, it was shown that amino-triazole calix[4]arenes bind to calf thymus DNA by classical intercalation. According to DLS and TEM data, all studied macrocycles cause significant DNA compaction, forming stable nanoparticles 50–20 nm in size. Among all studied calix[4]arenes the most lipophilic tetradecyl one proved to be the best for both binding and compaction of DNA. 相似文献
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简述了1,8-萘酰亚胺及其衍生物的合成方法,着重介绍其近年在荧光增白剂、荧光染料和其他功能材料上的应用研究进展,特别是在生物医用材料和荧光传感器等领域的应用。在生物医用材料方面的应用主要包括抗肿瘤药物、DNA荧光探针和荧光分子开关;而荧光传感器领域的应用则主要包括阴离子传感器和阳离子传感器。最后,展望了此类功能材料的发展趋势。 相似文献
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Jinguo Huang Ruiqi Zhao Jing Mo Fang Wang Dr. Xiaocheng Weng Xiang Zhou 《Chembiochem : a European journal of chemical biology》2021,22(9):1559-1562
There is growing interest in developing intracellular RNA tools. Herein, we describe a strategy for N3-kethoxal (N3K)-based bioorthogonal intracellular RNA functionalization. With N3K labeling followed by an in vivo click reaction with DBCO derivatives, RNA can be modified with fluorescent or phenol groups. This strategy provides a new way of labeling RNA inside cells. 相似文献