Uptake and balance of labelled fertilizer nitrogen by potatoes

Potatoes have a shallow rooting system. This can seriously affect the efficient use of fertilizer N. During two consecutive years, 1985 and 1986, a study was conducted on a commercial field to investigate the uptake of labelled N by potatoes under the recommended N rate and existing agricultural practices. The fertilizer efficiency, fertilizer distribution within the plant and soil and the total fertilizer balance were made using¹⁵NH₄ ¹⁵NO₃ 3.63 At. %¹⁵N excess. The recovery of the applied N-fertilizer in the whole plant was 25 and 56% for 1985 and 1986, respectively. The % Ndff and % Ndfs ranged between 30–40% and 60–70% respectively in both years. An important amount of fertilizer N was left in the soil after harvest. It reached 44 and 34% in 1985 and 1986, respectively.The total balance of the applied fertilizer N showed that up to 31 and 10% of the fertilizer N was lost during 1985 and 1986, respectively. The differences between the two growing seasons were mainly related to the method and timing of fertilizer N application and to the amount of rainfall.     

The effect of autumn applied 15N-labelled fertilizer on nitrate leaching in a cultivated soil during winter

Nitrogen (N) fertilizer applied in autumn to arable farm land raises concerns over affects on ground water quality. The contribution of autumn ¹⁵N-labelled fertilizer (50 kg N ha^-1) to nitrate leaching losses from a cultivated soil (silt loam on sandy loam; Udic Ustochrept) was measured using undisturbed monolith lysimeters (500 mm diameter, 700 mm long) during consecutive winters in Canterbury, New Zealand. The addition of ¹⁵N-labelled fertilizer at 50 kg N ha^-1 did not significantly increase nitrate leaching losses. Soil-derived-N contributed 78 and 88% (1996 and 1997, respectively) of the nitrate leached beneath fertilized lysimeters. Warmer weather and wetter soil conditions at cultivation and fertilizer application during 1997, compared with 1996, resulted in an increased release of soil-derived-N in 1997. Nitrate leaching and average nitrate concentrations were therefore 41% and 56% higher, respectively, during the winter of 1997 than the winter of 1996. However, fertilizer leaching losses were relatively consistent between years (7.8 and 8.6%). Although not statistically significant, total N leaching losses and average nitrate concentration were 24 to 30% higher below fertilized lysimeters as compared with unfertilized lysimeters, indicating a priming effect of fertilizer on soil N release. During both late winter periods, leachate nitrate concentrations from fertilized and unfertilized lysimeters exceeded World Health Organisation (WHO) limits for drinking water. Higher release of soil-derived-N in 1997 also meant WHO limits were exceeded for 6 weeks longer than in 1996. In conclusion, the application of ¹⁵N fertilizer in autumn directly contributed only a small proportion of the total amount of N leached in this cultivated soil. However, the apparent priming effect of autumn applied-N fertilizer has importance on the overall environmental impact of this production system, as the amount of N leached, and extent to which health limits were exceeded, was largely determined by the factors which controlled the release of soil-derived-N.     

Recovery of 15N-labelled fertilizer applied to winter wheat and perennial ryegrass crops and residual 15N recovery by succeeding wheat crops under different crop residue management practices

The recovery of ¹⁵N-labelled fertilizer applied to a winter wheat (120 kg N ha^–1) and also a perennial ryegrass (60 kg N ha^–1) crop grown for seed for 1 year in the Canterbury region of New Zealand in the 1993/94 season was studied in the field. After harvests, ryegrass and wheat residues were subjected to four different residue management practices (i.e. ploughed, rotary hoed, mulched and burned) and three subsequent wheat crops were grown, the first succeeding wheat crop sown in 1994/95 to examine the effects of different crop residue management practices on the residual ¹⁵N recovery by succeeding wheat crops. Total ¹⁵N recoveries by the winter wheat and ryegrass (seed, roots and tops) were 52% and 41%, respectively. Corresponding losses of ¹⁵N from the crop-soil systems represented by un-recovered ¹⁵N in crop and soil were 12% and 35%, respectively. These losses were attributed to leaching and denitrification. The proportions of ¹⁵N retained in the soil (0-400 mm depth) at the time of harvest of winter wheat and ryegrass were 36% and 24%, respectively. Although the soil functioned as a substantial sink for fertilizer N, the recovery of this residual fertilizer by subsequent three winter wheat crops was low (1-5%) and this was not affected by different crop residue management practices.     

Nitrogen fertilizer in leucaena alley cropping. II. residual value of nitrogen fertilizer and leucaena residues

Legume residues have been credited with supplying mineral nitrogen (N) to the associated cereal crop and improving soil fertility in the long term. Few studies using¹⁵N have reported the fate of legume N and fertilizer N in the presence of legume residues in soil-plant systems over periods of two years or longer. A field experiment was conducted in microplots to evaluate: (1) the residual value of the¹⁵N added in leucaena residues; (2) the residual value of fertilizer¹⁵N applied in the presence of unlabelled leucaena residues in the first year to maize over three subsequent years; and (3) the long-term fate of residual fertilizer and leucaena¹⁵N in a leucaena alley cropping system.There was a significant increase in maize production over three subsequent years after addition of leucaena residues. The residual effect of fertilizer N increased maize yield in the second year when N fertilizer was applied at 36 kg N ha^–1 in the first year in the presence of leucaena residues. Of the leucaena¹⁵N applied in the first year, the second, third and fourth maize crop recovered 2.6%, 1.8% and 1.4%, respectively. The corresponding values for the residual fertilizer¹⁵N were 0.7%, 0.4% and 0.3%. About 12–14% of the fertilizer¹⁵N added in the first year was found in the 200 cm soil profile over the following three years. This differed from the 38–41% of leucaena¹⁵N detected in the soil over the same period. Most of the residual fertilizer and leucaena¹⁵N in the soil was immobilized in the top 25 cm with less than 1% leached below 100 cm. More than 36% of the leucaena¹⁵N and fertilizer¹⁵N added in the first year was apparently lost from the soil-plant system in the first two years. No further loss of the residual leucaena and fertilizer¹⁵N was detected after two years.     

The effect of plant residues on plant uptake and leaching of soil and fertilizer nitrogen in a tropical red earth soil

Two field experiments, in which differing amounts and types of plant residues were incorporated into a red earth soil, were conducted at Katherine, N.T., Australia. The aim of the work was to evaluate the effect of the residues on uptake of soil and fertilizer N by a subsequent sorghum crop, on the accumulation and leaching of nitrate, and on losses of N.Stubble of grain sorghum applied at an exceptionally high rate (~ 18 000 kg ha^–1) reduced uptake of N by sorghum by 13% and depressed the accumulation of nitrate under a crop and particularly under a fallow.Loss of fertilizer N, movement of nitrate down the profile, and uptake by the crop was studied in another experiment after application of N as¹⁵NH₄ ¹⁵NO₃ to field microplots. By four weeks after fertilizer application 14% had been lost from the soil-plant system and by crop maturity 36 per cent had been lost. The pattern of¹⁵N distribution in the profile suggested that losses below 150 cm had occurred during crop growth. The recovery of¹⁵N by the crop alone ranged from 16 to 32 per cent. There was an apparent loss of N from the crop between anthesis and maturity. Residue levels common to sorghum crops in the region (~ 2000 kg ha^–1) did not significantly affect uptake by a subsequent sorghum crop, N losses, or distribution of nitrate in the profile.     

Detectability of15N-depleted fertilizer N in soil and plant tissue during a corn-rye crop rotation

Use of¹⁵N-depleted fertilizer materials have been primarily limited to fertilizer recovery studies of short duration. The objective of this study was to determine if¹⁵N-depleted fertilizer N could be satisfactorily used as a tracer of residual fertilizer N in plant tissue and various soil N fractions through a corn (Zea mays L.) -winter rye (Secale cereale L.) crop rotation. Nitrogen as¹⁵N-depleted (NH₄)₂SO₄ was applied at five rates (0, 84, 168, 252, and 336 kg N ha^–1) to corn. Immediately following corn harvest a winter rye cover crop treatment was initiated. Residual fertilizer N was easily detected in the soil NO ₃ ^- -N fraction following corn harvest (140-d after application). Low levels of exchangeable NH ₄ ⁺ -N (<2.5 mg kg^–1) did not permit accurate isotope-ratio analysis. Fertilizer-derived N recovered in the soil total N fraction following corn harvest was detectable in the 0 to 30-cm depth at each N rate and in the 30 to 60 and 60 to 90-cm depths at the 336 kg ha^–1 N rate. Atom %¹⁵N concentrations in the nonexchangeable NH ₄ ⁺ -N fraction did not differ from the control at each N rate. Nitrogen recovery by the winter rye cover crop reduced residual soil NO ₃ ^- -N levels below the 10 kg ha^–1 level needed for accurate isotope-ratio analysis. Atom %¹⁵N concentrations in the soil total N fraction (approximately one yr after application) were indistinguishable from the control plots below the 168, 252, and 336 kg ha^–1 N rate at the 0 to 30, 30 to 60, and 60 to 90-cm depths, respectively. Recovery of residual fertilizer N by the winter rye cover crop was verified by measuring significant decreases in atom %¹⁵N concentrations in rye tissue with increasing N rates. The greatest limitation to the use of¹⁵N-depleted fertilizer N as a tracer of residual fertilizer N in a corn-rye crop rotation appears to be its detectibility from native soil N in the total N pool.Research partially supported by grants from the National Fertilizer and Environmental Research Center/TVA and the Virginia Division of Soil and Water Conservation.     

Nitrogen fertilizer in leucaena alley cropping. I. Maize response to nitrogen fertilizer and fate of fertilizer-15N

Field microplot experiments were conducted in the semi-arid tropics of northern Australia to evaluate the response of maize (Zea mays L.) growth to addition of N fertilizer and plant residues and to examine the fate of fertilizer¹⁵N in a leucaena (Leucaena leucocephala) alley cropping system, in which supplemental irrigation was used. Leucaena prunings, maize residues and N fertilizer were applied to alley-cropped maize grown in microplots which were installed in the alleys formed by leucaena hedgerows spaced 4.5 metres apart. The¹⁵N-labelled fertilizer was used to examine the fate of fertilizer N applied in the presence of mulched leucaena prunings and maize residues.Application of leucaena prunings increased maize yield while addition of N fertilizer in the presence of the prunings produced a further increase in maize production. There was a significant positive interaction between N fertilizer and leucaena prunings in increasing maize production. The addition of maize residues in the presence of N fertilizer and leucaena prunings decreased maize yield and N uptake and increased fertilizer¹⁵N loss from 38% to 47%. Maize recovered 24–79% of fertilizer¹⁵N in one cropping season, depending on application rate of N fertilizer and field management of plant residues. About 20–34% of fertilizer¹⁵N remained in the soil. More than 37% of fertilizer¹⁵N was apparently lost from the soil and plant system largely through denitrification when N fertilizer was applied at 40 kg N ha^–1 or more in the presence or absence of plant residues. Application of N fertilizer improved maize yield and increased the contribution of mulched leucaena prunings to crop production in the alley cropping system.     

Effect of climate on the recovery in crop and soil of15N-labelled fertilizer applied to wheat

Data was assembled from experiments on the fate of¹⁵N-labelled fertilizer applied to wheat (Triticum spp.) grown in different parts of the world. These data were then ranked according to the annual precipitation-evaporation quotient for each experimental location calculated from the average long-term values of precipitation and potential evaporation. Percentage recovery of¹⁵N fertilizer in crop and soil varied with location in accordance with the precipitation-evaporation quotient. In humid environments more¹⁵N fertilizer was recovered in the crop than in the soil, while in dry environments more¹⁵N fertilizer was recovered in the soil than in the crop. Irrespective of climatic differences between locations 20% (on average) of the¹⁵N fertilizer applied to wheat crops was unaccounted for at harvest. Most of the¹⁵N fertilizer remaining in the soil was found in the 0–30 cm layer. The most likely explanation of these differences is that wheat grown in dry environments has a greater root:shoot ratio than wheat grown in humid environments and, further, that the residue of dryland crops have higher C/N ratios. Both factors could contribute to the greater recovery of¹⁵N fertilizer in the soil in dry environments than in humid ones.     

1. The chemistry and biology of flooded soils in relation to the nitrogen economy in rice fields

Response of lowland rice to sources and methods of nitrogen fertilizer application were summarized for more than 100 experiments. In about 2/3 of the experiments, the yield increase per kg of fertilizer N was judged to be relatively poor with best split applications of urea. Based on frequency distribution, sulfur coated urea and urea briquets or urea supergranules deep placed more often produced satisfactory yield increases than best split urea, but even with these sources/methods the yield increases were judged to be relatively poor in about 1/2 of the experiments. There is an enormous potential to increase rice production with no further increases in inputs of fertilizer N if we could learn to match the best method/source of fertilizer with the soil-crop management complex.About 60% of the yields with no fertilizer N were in the range of 2 to 4 t/ha. Based on the average yield response to urea, this is equivalent to about 100 kg of urea N. It would appear worthwhile to study ways to improve utilization of soil nitrogen since it is already in place on the land and apparently in fairly abundant amounts in many soils.About 50 experiments with¹⁵N fertilizers were summarized. In almost all cases, the uptake of tagged fertilizer was less than the net increase in N in the above ground matter. In about 2/3 of the experiments, the addition of fertilizer N increased soil N uptake more than 20% and in 1/3 of the experiments the uptake of soil N was increased more than 40%. These results lead to much uncertainty about practical interpretation and use of¹⁵N data.     

The comparative effects of ammonium nitrate,urea or a combined ammonium nitrate/urea granular fertilizer on the efficiency of nitrogen recovery by perennial ryegrass

The comparative effects of ammonium nitrate (AN), urea or a combined 1:1 (w/w) AN/urea granular fertilizer with two different fillers (CaCO₃ or silica) were investigated on the efficiency of dry matter production and¹⁵N recovery by perennial ryegrass grown in pots under controlled environmental conditions.There was no significant difference between CaCO₃ and silica as the filler and therefore no indication that the presence of CaCO₃ in the pellet enhanced N loss from urea. Ammonium nitrate was the most efficient N source and urea the least efficient in terms of all the parameters studied. The¹⁵N budget in shoots, roots and soil indicated that only 60% of the nitrogen from urea was recovered at the end of the experiment compared with 95% for AN. However, the % recovery of¹⁵N from urea was increased by 17% in the presence of AN whereas the % recovery of AN was decreased by 19% in the presence of urea. The combined 1:1 (w/w) AN/urea source therefore gave intermediate yields between AN and urea alone. The results indicate that an interaction occurred between AN and urea in the granule.     

Turnover of15N ammonium sulfate with dicyandiamide under aerobic and anaerobic soil conditions

The influence of the nitrification inhibitor dicyandiamide (DCD) on the turnover of¹⁵N-labelled ammonium sulfate (AS) was investigated in two soils under aerobic and waterlogged conditions. Nitrification of ammonium sulfate was markedly inhibited by addition of DCD in both soils. Up to 45% of the supplied N was transformed into a non-extractable N form, which only slowly released nitrogen over 147 or 264 days. This immobilization was higher in the presence of DCD than without DCD. In all aerobic experiments, the recovery was 100% ± max. 2.4%, indicating that no gaseous losses of N occurred.If aerobic preincubation of 28 or 42 days was followed by water-logging with H₂O or a solution of glucose, considerable N losses occurred only in presence of the carbohydrate. DCD retarded nitrification and thus reduced losses by denitrification from 61 to 15%.DCD application resulted in an increased immobilization of labelled N into the non-exchangeable soil N fraction. This amounted to more than 50% of the applied N, compared to 39% without DCD.The late Dr. Klaus Vilsmeier, a very dedicated and talented young scientist, died before he was able to finish completely the revised version of this article. We will always keep him in our minds and kindly remember his kind personality as well as his sense of humour and justice. Prof. Dr. Heiner Goldbach on behalf of all members of the department.     

Influence of delaying flood and preflood nitrogen application on dry-seeded rice

In the southern U.S. rice belt it is recommended that rice (Oryza sativa L.) grown in the dry-seeded, delayed flood cultural system have the preflood N fertilizer applied and the field flooded at the fourth to fifth leaf stage of plant development. The objective of this field study was to determine if delaying the flood and preflood N application past the fifth leaf stage was detrimental to rice total N and fertilizer¹⁵N uptake, total dry matter, and grain yield. This study was conducted on a Crowley silt loam (Typic Albaqualfs) and a Perry clay (Vertic Haplaquepts). The preflood N fertilizer and flood were delayed 0, 7, 14, or 21 d past the fourth to fifth leaf stage, after which time a permanent flood was established and maintained until maturity. All treatments received 20.5 g N m^–2 as¹⁵N-labeled urea in three topdress applications. All plant and soil samples were taken at maturity. Harvest index increased as the preflood N and flood were delayed past the 4 to 5 leaf stage. Total N in the grain + straw either decreased or showed a decreasing trend as the N and flood were delayed. Similarly, uptake of native soil N decreased as flood was delayed. Conversely, percent recovery of fertilizer N in the rice plant and the plant-soil system increased as the preflood N and flood were delayed. Rice grain yield was not significantly affected by delaying the preflood N and flood up to 21 d.Received....... . Published with permission of the Director of the Arkansas Agric. Exp. Stn. Project ARK01386. Supported in part by the Tennessee Valley Authority National Fertilizer and Environmental Research Center and the Arkansas Rice Research and Promotion Board.     

Uptake of organic nitrogen by eight dominant plant species in Kobresia meadows

¹⁵N-labelled glycine experiments were carried out in both a Kobresia pygmaea meadow and a Kobresia humilis meadow to investigate whether alpine plants can take up organic nitrogen directly from the soil and whether different plant species differ in this respect. Eight plant species were selected in the two meadows, five in the Kobresia humilis meadow and four in the Kobresia pygmaea meadow, with one common species. After 4 h following ¹⁵N injection, atom% excess ¹⁵N in the aboveground parts of Ptilagrostis concinna was about 0.012, higher than in the aboveground parts of the other three species in the Kobresia pygmaea meadow, while that in the aboveground parts of Festuca ovina was higher than in the aboveground parts of the other four species in the Kobresia humilis meadow. After 1 day all the values for atom% excess ¹⁵N were substantially higher, except in the aboveground parts of Gentiana straminea in the Kobresia pygmaea meadow and in the aboveground parts of Festuca ovina and Gentiana aristata in the Kobresia humilis meadow. One day after ¹⁵N injection, atom% excess ¹⁵N in the roots was higher than that in any of the aboveground parts. In the first 4 h, uptake rates of organic nitrogen by the four species in the Kobresia pygmaea meadow were in the range of 0–0.83 µmol g^–1 h^–1, with a value of 1.43 µmol g^–1 h^–1 for the roots. In contrast, those of five species and the roots in the Kobresia humilis meadows varied between 1.34–8.08 µmol g^–1 h^–1. Key species such as Kobresia pygmaea and Kobresia humilis showed a greater capacity to take up organic nitrogen than non-key species over a 5-day period. This implies that alpine plants can take up organic nitrogen from the soil, but uptake capacity varies widely among different species, and for the same species from different Kobresia meadows.     

7. The efficacy and loss of fertilizer N in lowland rice

Nitrogen fertilization is a key input in increasing rice production in East, South, and Southeast Asia. The introduction of high-yielding varieties has greatly increased the prospect of increasing yields, but this goal will not be reached without great increases in the use and efficiency of N on rice. Nitrogen enters a unique environment in flooded soils, in which losses of fertilizer N and mechanisms of losses vary greatly from those in upland situations. Whereas upland crops frequently use 40–60% of the applied N, flooded rice crops typically use only 20–40%. There is a great potential for increasing the efficiency of N uptake on this very responsive crop to help alleviate food deficits in the developing world.This article reviews current use of N fertilizers (particularly urea) on rice, the problems associated with rice fertilization, and recent research results that aid understanding of problems associated with N fertilization of rice and possible avenues to increase the efficiency of N use by rice.     

Efficiency of nitrogen fertilizer in the sugarcane-vertical system in Guadeloupe according to growth and ratoon age of the cane

Sugarcane is one of the main economic resources of Guadeloupe (France). Cane grown on the island's vertisols shows nitrogen deficiency which is accentuated with each successive ratoon. This deficiency could partially explain the observed decrease in yield. The present study, based on the isotopic N method applied to different ratoons in the field, was aimed at: (i) diagnosing the problem in the crop environment itself; and (ii) quantifying the fertilizer-N balance. The results indicated that decrease in yield and N absorption by the cane was related to ratoon number. The real utilization coefficient for the fertilizer (RUC%) ranged from 6 and 34%, and a high proportion (30–40%) of fertilizer-N was immobilized in the soil (NiS%) after the annual crop cycle. The N absorbed by the cane was essentially derived from the soil. Rainfall at the beginning of (re)growth determined crop development and N supply to the crop. When the water requirements of the crop are satisfied, nitrogen supply and cane yield can be improved in two ways: (i) by increasing the efficiency of the applied N fertilizer (RUC% and NiS%); and (ii) by maintaining the soil's capacity to supply cane with N. This implies maintaining and, if necessary, upgrading the structural state of the vertisols.     

Fate of nitrogen applied in different fertilizers to the surface of a calcareous soil in Syria

¹⁵N-labelled ammonium sulphate or¹⁵N-labelled urea were each applied in solutionat a rate of 30 kg N ha^-1 to the surface of 20soil cores (52 mm internal diameter × 100 mm deep)located on a field experiment at the ICARDA station,Tel Hadya, Syria. Recovery of ¹⁵N-label in theammonium, nitrate, organic and/or urea-N pools in thesoil was measured on days 0, 1, 2, 5 and 13 afterapplication. Total recovery of ¹⁵N was initially100%, but by day 13 after application it had declinedto 51% with urea and 73% with ammonium sulphate.Ammonium nitrate labelled either as ammonium or asnitrate was also applied to the soil surface of 8other cores at the same time. ¹⁵N recovery in thefour soil N pools was measured only on day 12 afterapplication. Total recovery of ¹⁵N-label was 75%with labelled ammonium and 57% with labelled nitrate.Volatilization of ammonia from this calcareous soil(pH 8.1) is one probable mechanism of N loss fromammonium and urea fertilizers: with nitrate bothleaching beyond the base of the core (i.e. 100 mm) and denitrification were responsible for Nlosses. These large losses of N immediately afterapplication have implications for fertilizermanagement practices.     

15N isotope dilution techniques to study soil nitrogen transformations and plant uptake

The use of¹⁵N as a tracer in soil/plant research is examined. The limitations of the so-called Ndff approach are discussed to show the need to consider not just the fate of the added label but also the path that was followed and the rate of the transformation. The development of¹⁵N isotope dilution techniques to determine gross rates of nitrogen transformation in soil is reviewed with some indications as to the further development of the approach.     

Evaluation of distilling the entire digest or an aliquot for total nitrogen determination in soil digests

Accurate and easy to adapt methods of total soil N determination are a prerequisite for N balance research. For¹⁵N balance studies certain modifications of the regular methods are generally adopted, e.g. the distillation of an aliquot of the digest in preference to the entire digest. However, comparative evaluation of such methods has not been investigated. In this study, three methods of distilling soil digests were evaluated for the determination of total N in diverse Alfisols and Vertisols. These are distillation of a clear aliquot (suspended materials allowed to settle) of the digest, distillation of an aliquot with suspended materials, following digestion in a block digestor, and distillation of the entire digest following macro-Kjeldahl digestion. The total N content of soils were determined to be similar whether the aliquot distilled was a clear solution or a suspension with solid materials, and these results were similar to those obtained by distilling the entire digest. The precision obtained by the three methods of distillation was similar for the Vertisols but for the Alfisols, distillation of the clear aliquot of the digest was found to be most precise.Submitted as Journal Article No. 776 by International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)     

Efficiency of urea and ammonium sulfate for wetland rice grown on an acid sulfate soil as affected by rate and time of application

A pot experiment was conducted in a greenhouse to assess the effect of rate and time of N application on yield and N uptake of wetland rice grown on a Rangsit acid sulfate soil (Sulfic Tropaquepts). Response of rice at N rates of 800, 1600 and 2400 mg N/pot (5 kg of soil) was compared between urea and ammonium sulfate when applied at two times: (i) full-rate basal at transplanting and (ii) one half at transplanting and one half at the PI stage. In addition, labelled¹⁵N sources were applied either at transplanting or at the PI stage to determine the nitrogen balance sheet in the soil/plant system.No significant difference in grain and straw yields between urea and ammonium sulfate at low rate was observed. At the higher N rates, urea produced higher yields than did ammonium sulfate regardless of timing. The highest yields were obtained when urea at the high N rate was applied either in a single dose or a split dose while lowest yields were observed particularly when ammonium sulfate at the same rate was applied. Split application of N fertilizer was shown to be no better than a single basal application. The occurrence of nutritional disorder, a symptom likely reflected by high concentration of Fe (II) in combination with soluble Al, was induced with high rate of ammonium sulfate.In terms of fertilizer N recovery by using¹⁵N-labelling, ammonium sulfate was more efficient than urea when both were applied at transplanting. In contrast, application at the PI stage resulted in higher utilization of urea than of ammonium sulfate. The recovery of labelled N in the soil was higher with urea than with ammonium sulfate when the two sources were applied at transplanting, while the opposite result was obtained when the same fertilizers were applied at the PI stage. The losses from urea and ammonium sulfate were not different when these fertilizers were applied at transplanting but loss from urea was higher than that from ammonium sulfate when both were applied at the PI stage.     

The fate of algal nitrogen in a flooded soil system

Algal N labelled with ¹⁵N added to a flooded soil in laboratory columns without plants was studied to determine the changes over time in the fate of N assimilated by algae and to study how its fate is affected by (a) exclusion of light simulating complete closure of the rice canopy, and (b) addition of fertilizer-NH₄^*. In the light but with no added fertilizer-N there was little net mineralization of the added algal N during the first 4 weeks, but after 8 weeks 42% had been mineralized, of which 95% was denitrified. Exclusion of light caused net mineralization to proceed more rapidly in the first 4 weeks due to the death of algal cells and lowered reassimilation. After 8 weeks 51% had been mineralized, of which 54% was denitrified, 16% volatilized and 30% was present as KCl exchangeable NH₄⁺-N. Application of fertilizer-NH₄⁺ apparently caused mineralization of 25% of the algal N within one week but the results were probably affected by pool substitution in which labelled N mineralized to NH₄⁺-N was diluted with fertilizer – NH⁺₄ and then immobilized leaving more labelled NH₄–N in the mineral pool. After 8 weeks, 42% of algal N had been mineralized, of which 69% was estimated to have been denitrified, 19% lost through NH₃ volatilization and 12% remained as extracted NH₄⁺+NO^-₃. Uptake of N by a rice crop would reduce the gaseous losses. Algal N was mineralized quickly enough to be available during the growing season of a rice crop and, depending on field conditions, algae may have a role in assimilating N and protecting it from loss as well as being a major driving force for NH₃ volatilization through diurnal increases in pH.