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1.
Jin‐Won Lee Seog‐Won Lee Min‐Kyung Kim Chul Rhee In‐Hwan Kim Kwang‐Won Lee 《Journal of the science of food and agriculture》2005,85(3):493-498
Unsaponifiable matter (UM) was prepared from rice bran using n‐hexane extraction followed by removal of its fatty acid methyl ester with supercritical CO2 under heat‐stable conditions. The UM was made up of 1% of vitamin E isomers, 28% of γ‐oryzanol and 71% of uncharacterized compounds. The aim of this study was to determine the antioxidant activities of the UM, using α‐tocopherol (α‐T) as a positive control, by measuring the Fe3+‐reducing antioxidant power (FRAP), 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH?) free‐radical‐scavenging property and lipid peroxidation in rat liver microsomes. In addition, the effects of the UM on the tert‐butyl hydroperoxide (t‐BOOH)‐induced cytotoxicity in cultured rat hepatocytes were also investigated. In FRAP assay and DPPH? free‐radical‐scavenging assay, the results were expressed g?1α‐T or g?1 UM. The amount of UM used in lipid peroxidation assay and cytotoxicity assay was the amount required to have equal amounts of total vitamin E isomers in the sample and the control α‐T. The UM, as well as α‐T, exhibited significant antioxidant activities in FRAP, radical‐scavenging and membrane‐lipid oxidation. The FRAP value for total vitamin E isomers in the UM (TVEIUM) was 9.1 times higher than that for α‐T. In terms of their capacities to perform radical‐scavenging and lipid peroxidation, both TVEIUM and α‐T showed similar antioxidant activities. In experiments using cultured rat hepatocytes, the t‐BOOH‐induced lactate dehydrogenase release was significantly inhibited by the addition of 63.5 and 160 µg ml?1 of TVEIUM treatments (84 and 89%, respectively), and that of 63.5 and 160 µg ml?1 of α‐T treatment (88 and 93%, respectively). The antioxidant function against oxidative stress of the UM prepared from rice bran may extend its use to being a potential dietary supplement. Copyright © 2004 Society of Chemical Industry 相似文献
2.
Jasna M. anadanovi-Brunet Sonja M. Djilas Gordana S. etkovi Vesna T. Tumbas Anamarija I. Mandi & Vladimir M. anadanovi 《International Journal of Food Science & Technology》2006,41(6):667-673
The sequential extraction of Teucrium montanum L. was realised with five solvents of different polarities (70% methanol, petroleum ether, chloroform, ethyl acetate, and n‐butanol) and HPLC method was used for identification of phenolic compounds. The total phenolic content of the extracts was determined spectrophotometrically according to the Folin–Ciocalteau procedure and range from 0 to 296 mg g?1. The antioxidant activity of extracts was tested by measuring their ability to scavenge reactive hydroxyl radical during the Fenton reaction, using electron spin resonance (ESR) spectroscopy. Moreover, the influence of these extracts on lipid peroxyl radicals obtained during lipid peroxidation of: (1) sunflower oil (37 °C, 3 h) induced by 4,4′‐azobis(4‐cyanovaleric acid) (ACVA) and (2) liposomes induced by 2,2′‐azobis(2‐amidino‐propane)dihydrochloride (AAPH) was studied. n‐Butanol extract, because of the highest content of total phenolic compounds (296 mg g?1) had the best antioxidant activity (100% at 0.16 mg mL?1 in Fenton reaction system; 90.57% at 5 mg mL?1 in system I; 100% at 5 mg mL?1 in system II). 相似文献
3.
Total phenol,flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables 总被引:1,自引:0,他引:1
Theeshan Bahorun Amitabye Luximon‐Ramma Alan Crozier Okezie I Aruoma 《Journal of the science of food and agriculture》2004,84(12):1553-1561
Ten Mauritian vegetables, broccoli, cauliflower, white cabbage, lettuce, Chinese cabbage, mugwort, carrot, onion, tomato and chilli pepper, were analysed for their total phenol, flavonoid, proanthocyanidin and vitamin C contents and antioxidant capacity. Antioxidant activities of the vegetables ranged from 0.43 to 3.68 µmol g?1 fresh weight Trolox equivalent antioxidant capacity (TEAC) and from 0.60 to 8.47 µmol g?1 fresh weight ferric reducing antioxidant power (FRAP). Levels of total phenols in the vegetables varied between 132 and 1189 µg g?1 fresh weight and those of total flavonoids between 45 and 944 µg g?1 fresh weight, while proanthocyanidins were detected at very low levels in only a few vegetables. Vitamin C contents varied between 25 and 748 µg g?1 fresh weight. Quercetin was the dominant flavonoid aglycone in the hydrolysed vegetable extracts, with values in the range of 15–390 µg g?1 fresh weight. There were strong correlations between antioxidant capacity and total phenols (TEAC, r = 0.91; FRAP, r = 0.83) and total flavonoids (TEAC, r = 0.89; FRAP, r = 0.82). Vitamin C contents showed poor correlation with TEAC values (r = 0.33), while no correlation was observed with FRAP values. Highest antioxidant capacities were observed in Chinese cabbage (Brassica chinensis L), onion (Allium cepa L), mugwort (Artemisia vulgaris Cantley) and broccoli (Brassica oleracea L var botrytis L subvar cymosa). Mauritian vegetables therefore represent a significant source of phenolic antioxidants, with quercetin derivatives being most abundant, and this may contribute to their potential health benefits. Copyright © 2004 Society of Chemical Industry 相似文献
4.
Nigel Deighton Rex Brennan Chad Finn Howard V Davies 《Journal of the science of food and agriculture》2000,80(9):1307-1313
The antioxidative capacities of a number of Rubus species of varied pigmentation have been investigated. In addition, total phenol, anthocyanin and ascorbic acid contents have been determined. Two methods to assess the antioxidant potential of fruit juices have been used. The antioxidant capacities of the fruit ranged from 0 to 25.3 µmol Trolox equivalents g−1 (TEAC) or from 190 to 66 000 µmol l−1 ferric reducing antioxidant power (FRAP). Ascorbic acid contributes only minimally to the antioxidant potential of Rubus juices (<10%, TEAC). There are apparent linear relationships between antioxidant capacity (assessed as both TEAC and FRAP) and total phenols (rxy = 0.6713 and 0.9646 respectively). Also, anthocyanin content has a minor influence on antioxidant capacity (rxy = 0.3774, TEAC; rxy = 0.5883, FRAP). The sample with the highest antioxidant capacity (Rubus caucasicus) had the highest phenol content, but only a low percentage was represented by anthocyanins. The present study demonstrates the potential of certain wild Rubus species, notably R caucasicus, for improvement of nutritional value through germplasm enhancement programmes. © 2000 Society of Chemical Industry 相似文献
5.
Antioxidant actions and phenolic and vitamin C contents of common Mauritian exotic fruits 总被引:1,自引:0,他引:1
Amitabye Luximon‐Ramma Theeshan Bahorun Alan Crozier 《Journal of the science of food and agriculture》2003,83(5):496-502
Seventeen commonly consumed exotic fruits from Mauritius were analysed for their antioxidant capacity, total phenolics, proanthocyanidins, flavonoids and vitamin C content. Two independent methods were used to evaluate the antioxidant potential of total fruit extracts. The antioxidant activities of the fruits ranged from 1 to 47 µmol Trolox equivalent antioxidant capacity (TEAC) g?1 fresh weight and from 0.3 to 34 micro/mol g fresh weight (FRAP) g?1 fresh weight. Total phenolics in the fruits ranged from 118 to 5638 µg g?1 fresh weight, proanthocyanidins from 7 to 2561 µg g?1 fresh weight, flavonoids from 21 to 712 µg g?1 fresh weight and vitamin C content from 8 to 1426 µg g?1 fresh weight. There were strong correlations between antioxidant activity (assessed by both TEAC and FRAP) and total phenolics and proanthocyanidins. Flavonoids seemed to contribute less to the antioxidant potential of the fruits, while very poor correlations were observed between ascorbate content and antioxidant activity. The highest antioxidant capacities were observed in red and yellow Psidium cattleianum Sabine ‘Chinese guava’, sweet and acid Averrhoa carambola L ‘starfruit’, Syzygium cumini L Skeels ‘jamblon’ and white Psidium guajava L ‘guava’. These fruits were also characterised by high levels of total phenolics. Mauritian exotic fruits are thus a significant source of phenolic antioxidants, which may have potential beneficial effects on health. Copyright © 2003 Society of Chemical Industry 相似文献
6.
Ray‐Yu Yang Samson CS Tsou Tung‐Ching Lee Wan‐Jan Wu Peter M Hanson George Kuo Liwayway M Engle Po‐Yung Lai 《Journal of the science of food and agriculture》2006,86(14):2395-2403
Epidemiological studies have demonstrated the protective effects of fruit and vegetable consumption on human health. A total of 150 edible plants representing 127 species were evaluated for Trolox equivalent antioxidant capacity (TEAC) and superoxide scavenging (SOS) activity. Distributions of vegetables for TEAC and SOS were not continuous but highly skewed. TEAC values on a dry weight basis ranged from 0 to 2105 µmol TE g−1 and SOS values ranged from 0 to 6206 µmol ascorbate equivalent (AE) g−1. The majority of vegetables clustered within 200 µmol TE g−1 and 400 µmol AE g−1. Vegetable species were grouped into six classes after natural logarithm transformation of TEAC and SOS values. Most highly consumed vegetables fell in the very low to medium classes. Species in the very high class for both TEAC and SOS included leaves of Cedrela sinensis (Chinese cedar), Oroxylum indicum (Damocles tree), Cassia siamea (cassod tree) and Cassia tora (sickle senna). Edible plants high in antioxidant activity (AOA) were mostly perennial and underutilised crops, while commonly consumed vegetables were generally low in AOA. There is great potential to increase dietary antioxidants and improve human health through consumption of diverse vegetable species, especially those high in AOA. Copyright © 2006 Society of Chemical Industry 相似文献
7.
B Dave Oomah Anaberta Cardador‐Martínez Guadalupe Loarca‐Pia 《Journal of the science of food and agriculture》2005,85(6):935-942
Six bean cultivars grown in southern Manitoba for 2 years were evaluated for variability in yield of millstreams and phenolic constituents. The ethanolic extract of bean cultivars and millstreams was screened for antioxidant activity using the β‐carotene‐linoleate and the 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) in vitro model systems. Cultivar was the main source of variation for yield of millstreams, content of phenolic compounds and antioxidant activities. Phenolic compounds in cultivars varied from 3.3 to 16.6 mg catechin equivalent and from 0.15 to 0.32 mg cyanidin‐3‐glucoside equivalent g?1 bean for total phenolic and anthocyanin contents, respectively. The bean cultivars exhibited antioxidant activity (AA) of 10–46% inhibition of lipid peroxidation in the linoleate and 0.4–1.3 trolox equivalent antioxidant capacities (TEAC) in the DPPH model systems. The hull millstream with maximum concentration of phenolic compounds exhibited the strongest antioxidant activity of 383 µM trolox equivalent g?1 hull. Total phenolic content, alone or in combination with other phenolic constituents, is a potential candidate as a selection criterion for antioxidant activity in beans. Copyright © 2005 Society of Chemical Industry 相似文献
8.
Total phenolic content (PC) was ~12 mg g?1 in lentils, 2.2 mg g?1 in chickpeas, 2.3 mg g?1 in soybeans, 2.5 mg g?1 in yellow peas and 1.2 mg g?1 in green peas. Total antioxidant activity (AA) determined by ABTS (2,2′‐azinobis‐3‐ethyl‐benzthiazoline‐6‐sulfonic acid) assay was highest in lentils at around 14 μmol Trolox equivalent antioxidant capacity (TEAC) g?1 and lowest in green peas at 1.9 μmol TEAC g?1. Bound phytochemicals contributed to 82–85% total AA in lentils. Free phytochemicals contributed more to total AA in chickpeas, yellow peas, green peas and soybeans than bound phytochemicals. AA and PC was reduced by ~80% in lentils and <30% in yellow peas by decortication, by 16–41% in lentils, chickpeas and peas by cooking, and by 22–42% in lentils by soaking. Total AA was significantly correlated with total PC. Soybeans had the greatest ability to scavenge free radicals, inhibit lipid peroxidation and chelate metals among the legumes tested. Different legumes exhibited different AA mechanisms. 相似文献
9.
Guadalupe Yeraldin Medina-Saavedra José Andrés Herrera-Corredor Yamill Vargas-Rivera Oscar Valeriano Sánchez-Valera Adán Cabal-Prieto Witoon Prinyawiwatkul Emmanuel de Jesús Ramírez-Rivera Lorena Guadalupe Ramón-Canul 《International Journal of Food Science & Technology》2021,56(7):3322-3332
The biological activity of mango leaf extracts from different mango varieties was evaluated in terms of total phenolic content, flavonoids, antioxidant activity and inhibition of α-amylase and α-glucosidase enzymes. Leaf extract-based beverages were formulated and evaluated for some physicochemical, microbiological and sensory properties. Results indicated that the extract from 'Tommy Atkins' mango leaves had the highest total phenols content (137.08 mg of GAE g−1) and antioxidant activity (DDPH = 38.26 mg TEAC 100 g−1; ABTS = 59.13 mg TEAC 100 g−1). The beverage formulated with 20% leaf extract presented the highest percentage of antioxidant activity (38.63%) and inhibition of enzymes α-amylase (41.9%) and α-glucosidase (37.53%). All beverages presented a yellow hue and consumers rated it with a degree of liking between 4.8 and 7.3 according to a hedonic scale. Results showed that the biological properties of beverages could be an alternative for the control of free radicals and glucose levels. 相似文献
10.
Amin ZA Abdulla MA Ali HM Alshawsh MA Qadir SW 《Journal of the science of food and agriculture》2012,92(9):1874-1877
BACKGROUND: Recently much attention has been paid to biologically active plants because of their low production cost and fewer adverse effects compared with chemical drugs. In the present investigation the bioactivity of Phyllanthus niruri ethanol and aqueous extracts was evaluated in vitro. RESULTS: The ethanol extract of P. niruri showed a high level of flavonoid content (123.9 ± 0.002 mg g?1), while the aqueous extract showed the highest 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH; IC506.85 ± 1.80 µmol L?1) and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS; 46.44 ± 0.53 µmol L?1) free radical scavenging activities with high phenol content (376 ± 0.02 mg g?1) and elevated levels of ferric reducing antioxidant power (FRAP; 23 883 ± 0.019 mmol g?1) with excellent antibacterial activity against Staphylococcus aureus (20 mm inhibition zone) and Streptococcus agalactiae (12 mm inhibition zone), respectively, in addition to the best immune activation potential of human peripheral blood mononuclear cells (450.5%). CONCLUSIONS: It is clear from our results that both extracts of P. niruri has excellent bioactivity roles via elevated levels of antibacterial, antioxidant and percentage of peripheral blood mononuclear cell proliferation, which could lead to the development of medications for clinical use. Copyright © 2012 Society of Chemical Industry 相似文献
11.
Olga Laporta Laura Pérez-FonsRicardo Mallavia Nuria CaturlaVicente Micol 《Food chemistry》2007,101(4):1425-1437
The rhizome of the plant Hypoxis rooperi (“African potato”) is known for its traditional and ethnomedical uses in the treatment of benign prostatic hyperplasia and other diseases. We have characterized an extract derived from H. rooperi, isolated its major bioactive compound, hypoxoside, and obtained its aglycone, rooperol, by enzymatic digestion. Absorption, fluorescence emission and bidimensional NMR complete spectral data of these compounds were obtained. The antioxidant capacity of both compounds was fully analyzed through the thiobarbituric acid reactive substances (TBARS) and Trolox equivalent antioxidant capacity (TEAC) assays, and it was compared to catechins and olive biophenolics. Both compounds showed a strong antioxidant capacity, although rooperol exhibited a higher antioxidant activity against lipid peroxidation which correlated to its strong affinity for phospholipid membranes as derived from its extremely high lipid/water partition coefficient (Kp = 3.4 × 104). The study of the lipophilic (EtOH) and hydrophilic (water) TEAC values revealed that more hydrophobic compounds, had greater lipophilic TEAC values than hydrophilic ones, probably indicating that lipophilic TEAC assay may be more reliable for these compounds. The H. rooperi extract also showed higher antioxidant efficacy compared to other strong antioxidant herbal extracts, such as olive leaf or green tea. Moreover, neither evidence of acute oral toxicity nor adverse effects were observed when the H. rooperi commercial extract containing 45% hypoxoside was used at a dosage of 2000 mg/kg. The results obtained in this work may contribute to understanding the biological activity described for these dicatechols and the African potato extract for food and cosmetic applications. 相似文献
12.
Palanisamy Marimuthu Chi‐Lin Wu Hui‐Ting Chang Shang‐Tzen Chang 《Journal of the science of food and agriculture》2008,88(8):1400-1405
BACKGROUND: Chamaecyparis obtusa var. formosana (Taiwan hinoki) is an endemic conifer in Taiwan and the purpose of this study is to evaluate the antioxidant activity of various fractions obtained from the bark of this plant material. The ethanolic extract of the bark was sequentially separated into three fractions, including n‐hexane, ethyl acetate and ethanol soluble fractions, by liquid–liquid partition. Then the antioxidant activities of crude extract and three fractions along with 13 subfractions obtained from the ethyl acetate (EA) soluble fraction were tested for several antioxidant assays. RESULTS: The total phenolic content of the samples varied from 27.71 to 102.86 mg GAE g?1 dry weight for fractions, and from 49.94 to 206.46 mg GAE g?1 for subfractions (where GAE is milligrams of gallic acid per gram of extract). The Trolox equivalent antioxidant capacity (TEAC) ranged from 0.15 to 0.26 mmol L?1 Trolox equivalents. The EA soluble fraction was found to be the best antioxidant‐rich fraction in terms of DPPH and reducing power assays. With further data analysis it was found that there was a positive correlation between the total phenolic content of extracts and TEAC is R2 = 0.61. CONCLUSION: Results from various antioxidant assays showed that the EA fraction possessed strong antioxidant activity. This would provide additional information about the antioxidant activity of bark extract of this plant species. Copyright © 2008 Society of Chemical Industry 相似文献
13.
Kaisu Mtt Anna‐Maija Lampi Jan Petterson Birgitta M Fogelfors Vieno Piironen Afaf Kamal‐Eldin 《Journal of the science of food and agriculture》1999,79(7):1021-1027
The total and individual sterol content in 21 oat samples (seven cultivars grown at three different locations in Sweden) were analysed by gas chromatography after acid hydrolysis. The total sterol content in these oat cultivars varied between 350–491 µg g−1 of dry weight of kernel. The most abundant phytosterol was β‐sitosterol (237–321 µg g−1) followed by campesterol (32–46 µg g−1), Δ5‐avenasterol (15–47 µg g−1) and stigmasterol (11–21 µg g−1). There was a statistically significant difference in total sterol content between cultivars (p < 0.05) but no effect was found for cultivation location. Furthermore when contents of Δ5‐avenasterol in hexane‐extracted oat oil and acid‐hydrolysed oat samples were compared, it was noticed that the content of Δ5‐avenasterol was lowered due to acid hydrolysis. © 1999 Society of Chemical Industry 相似文献
14.
This study aimed to identify the least aggressive and highest yield extraction method to obtain bioactive compounds from Inga marginata Willd fruits, determine the chemical components, and evaluate the extracts’' antimicrobial and cytotoxic activity. The extraction efficiency was expressed by the total phenolic and total flavonoid content and antioxidant activity (DPPH, IC50, and ORAC) using conventional, ultrasound, and microwave-assisted extraction. The highest bioactive compound content was achieved using 5 min at 60 °C for total phenolic content (214.98 mg GAE g−1), total flavonoid content (22.90 mg EQ g−1), DPPH (45.98 μmol TEAC g−1), inhibitory capacity (0.80 mg mL−1), and ORAC (167.25 μmol Trolox g−1) using ultrasonic extraction, and the extract inhibited the growth of all microorganisms tested. Thirteen chemical compounds were determined by ESI-ToF-MS, confirming the high phytochemical capacity of the extract. Lastly, the Inga extract showed no cytotoxicity at the concentrations used. 相似文献
15.
Gow‐Chin Yen Hui‐Yin Chen Chi‐En Lee 《Journal of the science of food and agriculture》1999,79(9):1213-1217
This study aims to investigate the acceleration effects of various prooxidants on linoleic acid peroxidation and to assess the feasibility of applying these model systems by measuring the antioxidant activities of butylated hydroxyanisole (BHA), α‐tocopherol and rosemary extracts. According to our results, Fe2+ and Fe3+ exhibited more stimulatory effects than other prooxidants on the peroxidation of linoleic acid. An additional amount of ascorbic acid can reduce Fe3+ to Fe2+, resulting in more severe peroxidation. However, ascorbic acid displays antioxidant activity with increasing concentration. The peroxidation of linoleic acid induced by Fenton reaction is less than that induced by iron ions and was increased by addition of ascorbic acid to the system to maintain the regeneration of Fe2+ and the production of hydroxyl radicals. BHA shows the strongest inhibitory effects on the peroxidation of linoleic acid in all systems. The antioxidant activity of α‐tocopherol is less than that of rosemary extracts in the iron ion‐induced peroxidation systems. However, α‐tocopherol shows stronger inhibitory effects than rosemary extracts in the Fenton reaction‐induced peroxidation system at a concentration less 100 mg kg−1. These systems can be useful in screening antioxidants from natural sources because of the short reaction time and clear difference between each antioxidant. © 1999 Society of Chemical Industry 相似文献
16.
Charles O. Piggott Alan Connolly Richard J. FitzGerald 《International Journal of Food Science & Technology》2014,49(10):2252-2259
Two types of brewers' spent grain (BSG), pale and black, were studied employing ultrafiltration (UF cut‐off, 10 kDa) to obtain high‐molecular‐weight (HMW) and low‐molecular‐weight (LMW) phenolic fractions from one pale (P2) and one black (B2) BSG extracts. Of the four UF fractions obtained, the HMW B2 fraction had highest level of polyphenols (5.73 ± 0.25 mg GAE g?1 BSG dw) and protein (18.82 ± 0.41 mg protein g?1 BSG dw). Metal‐chelating ability and antioxidative properties were also identified. Antioxidant activity evaluated using the DPPH and FRAP assays, indicated that activity in B2 extracts was associated mainly with the HMW fraction (3.10 ± 0.10 and 2.49 ± 0.09 TE g?1 BSG dw, respectively), whereas in P2 samples, antioxidant activity was highest in LMW fractions (0.58 ± 0.01 and 0.92 ± 0.03 TE g?1 BSG dw, respectively). The high antioxidant activity and metal‐chelating ability observed for black BSG extracts may be attributed to the presence of coloured melanoidinic structures. 相似文献
17.
Antioxidant potency of cumin varieties—cumin, black cumin and bitter cumin—on antioxidant systems 总被引:1,自引:0,他引:1
Cumin is one of the commonly used spices in food preparations. It is also used in traditional medicine as a stimulant, a carminative and an astringent. In this study, we characterized the antioxidant activity of three commercially available cumin varieties, viz., cumin (Cuminum cyminum), black cumin (Nigella sativa) and bitter cumin (C. nigrum). The antioxidant capacity of cumin varieties was tested on Fe2+ ascorbate induced rat liver microsomal lipid peroxidation, soybean lipoxygenase dependent lipid peroxidation and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging methods. The total phenolic content of methanolic extracts of cumin varieties ranged from 4.1 to 53.6 mg g–1 dry weight. Methanolic extracts of all the three varieties of cumin showed higher antioxidant activity compared with that of the aqueous extract. Among the cumin varieties, bitter cumin showed the highest antioxidant activity followed by cumin and black cumin in different antioxidant systems. IC50 values of the methanolic extract of bitter cumin were found to be 0.32, 0.1 and 0.07 mg dry weight of cumin seeds on the lipoxygenase dependent lipid peroxidation system, the DPPH radical scavenging system and the rat liver microsomal lipid peroxidation system, respectively. The data also show that cumin is a potent antioxidant capable of scavenging hydroxy, peroxy and DPPH free radicals and thus inhibits radical-mediated lipid peroxidation. The high antioxidant activity of bitter cumin can be correlated to the high phenolic content among the three cumin varieties. Thus, bitter cumin with a high phenolic content and good antioxidant activity can be supplemented for both nutritional purposes and preservation of foods. 相似文献
18.
EVANGELIA KARVELA DIMITRIS P. MAKRIS NICK KALOGEROPOULOS VAIOS T. KARATHANOS 《Journal of food quality》2011,34(5):299-305
ABSTRACT
A series of extracts from vinification by‐products, generated under specified, optimal conditions, were tested for their efficiency to inhibit lipid peroxidation in lecithin liposomes at various pH values. Lipid peroxidation was assessed by measuring the kinetics of CDV onset, which over a period of 6 days was found to obey zero‐order kinetics. As positive control samples the synthetic antioxidant, butylated hydroxytoluene and the natural quercetin were also considered. The results obtained showed that the most distinct inhibition as compared with control sample (no antioxidant added) was found upon addition of grape stem extract from the variety Vitis vinifera var. Moschofilero at pH 8, with the kinetics constant (k) being 6.6 × 10−2 CDV/d. By contrast, seed extract from the same variety at pH 2 was prominently prooxidant, with k = 50.7 × 10−2 CDV/d. Extracts from both stems and seeds exhibited large variations as a function of pH, with stem extracts being in general more efficient than seed extracts, but no consistent trend in response to pH was observed. This outcome clearly pointed to efficiency dependence upon both the composition of the extracts and pH.PRACTICAL APPLICATIONS
Because the use of antioxidants in food industry is indispensable, the study of the efficiency of certain widely used plant extracts possessing antioxidant properties becomes imminent. This is because the deployment of natural antioxidants in food matrices has often been accompanied by major discrepancies concerning their efficiency to shield against rancidity. Thus, revealing details on the antioxidant behavior of natural extracts with regard to significant food characteristics, such as pH, is of undisputed importance in utilizing these extracts in a technologically rational way. The results from this study showed that extracts from vinification by‐products, and presumably other extracts from various plant sources, might have variable behavior with regard to inhibiting and/or promoting lipid peroxidation. As this might depend on the pH of the matrix used or other intrinsic factors, the antioxidant potency of a particular extract can be reliably assessed only by case experimentation.19.
K Nagendra Prasad Soundar Divakar Gyarahally R Shivamurthy Somaradhya Mallikarjuna Aradhya 《Journal of the science of food and agriculture》2005,85(9):1461-1468
Ipomoea aquatica Forsk, a green leafy vegetable that is a rich source of vitamins and amino acids with many health benefits, has been explored for the isolation and identification of its bioactive compounds. Activity‐guided repeated fractionation of a methanol extract on a silica gel column followed by an XAD column yielded a compound that exhibited antioxidant activity with an EC50 value of 83 ± 1.02 µg ml?1 reaction mixture. It also showed very strong lipid peroxidation‐inhibitory activity in a liposome model system with an EC50 value of 72.2 ± 0.9 µg ml?1. However, it showed negligible metal‐chelating activity. Based on UV, 2D nuclear magnetic resonance and gas chromatography/mass spectrometry studies, the compound was tentatively identified to be 7‐O‐β‐D ‐glucopyranosyl‐dihydroquercetin‐3‐O‐α‐D ‐glucopyranoside. This is the first report on the antioxidant properties of I aquatica leaf extracts. Copyright © 2005 Society of Chemical Industry 相似文献
20.
Xiaonan Lu Jun Wang Hamzah M. Al-Qadiri Carolyn F. Ross Joseph R. Powers Juming Tang Barbara A. Rasco 《Food chemistry》2011
Total phenolic content (TPC) and total antioxidant capacity (TAC) of four onion varieties (red, white, yellow and sweet) and shallot from selected locations (Washington, Idaho, Oregon, Texas and Georgia) were determined using Fourier transform infrared (FT-IR) spectroscopy (4000–400 cm−1). The Folin–Ciocalteu (F–C) assay was used to quantify TPC and three assays were used to determine TAC, including 2,2-diphenyl-picrylhydrazyl (DPPH) assay, Trolox equivalent antioxidant capacity (TEAC) assay and ferric reducing antioxidant power (FRAP) assay. Partial least squares regression (PLSR) with cross-validation (leave-one-out) was conducted on onion and shallot extracts (n = 200) and their corresponding F–C, DPPH, TEAC and FRAP values were employed to obtain four independent calibration models for predicting TPC and TAC for the extracts. Spectra from an extra 19 independent extracts were used as an external validation set for prediction. A correlation of r > 0.95 was obtained between FT-IR predicted and reference values (by F–C, DPPH, TEAC and FRAP assay) with standard errors of calibration (SEC) and standard errors of cross-validation (SECV) less than 2.85, 0.35 and 0.45 μmol Trolox/g FW of extracts for TEAC, FRAP and DPPH assay, respectively; and 0.36 mg gallic acid/g FW of extracts for the F–C assay. In addition, cluster analysis (principal component analysis (PCA)) and discriminant function analysis (DFA) could differentiate varieties of onions and shallot based upon infrared spectral features. Loading plots for the various chemometrics models indicated that hydroxyl and phenolic functional groups were most closely correlated with antioxidant capacity. The use of mid-infrared spectroscopy to predict the total antioxidant capacity of vegetables provides a rapid and precise alternative to traditional wet chemistry analysis. 相似文献