Sulfuryl fluoride fumigation effects on the safety,volatile composition,and sensory quality of dry cured ham

A randomized complete block design with three replications was utilized to evaluate the effects (P < 0.05) of various fumigation concentrations of sulfuryl fluoride (SF) (0, 12, 24, 36 and 72 mg/L) on the fluoride, sulfuryl fluoride, and volatile flavor compound concentrations in dry cured ham. Fluoride and SF concentrations increased (P < 0.05) in dry cured hams as SF fumigation concentration increased, but all samples contained fluoride and SF concentrations below the legal limits of 20 and 0.01 ppm, respectively. Minimal differences existed (P > 0.05) in the presence and concentration of aroma active compounds, but oxidation compounds were slightly more prevalent (P < 0.05) in the 72 mg/ml treatment when compared to the control. A triangle test (n = 54) indicated that consumers could not discern (P > 0.75) between hams that were fumigated with SF at 0 and 36 mg/ml. This study revealed that there were minimal aroma/flavor differences among the ham treatments between 0 and 36 mg/ml, and that dry cured ham that was fumigated with SF was safe and met legal requirements for consumption.     

Texture and appearance of dry cured ham as affected by fat content and fatty acid composition

The relationship between texture and appearance characteristics and the moisture, intramuscular fat (IMF) content and fatty acid composition of dry cured Iberian ham was studied using principal component analysis. Intramuscular fat content positively influenced the proportion of oleic acid (R=0.5578), and exhibited a negative relationship with linoleic and arachidonic acids (R=−0.5215 and −0.5770 respectively). IMF content also had a positive influence on some texture and appearance traits of ham, such as oiliness, brightness, juiciness and marbling. However, it was negatively related to dryness, fibrousness and hardness. Moisture displayed no relationship with juiciness, dryness or any other textural or appearance trait. Therefore, within the ranges of fat content and moisture found in the present study, IMF content has a stronger influence on the texture of ham than the degree of dehydration.     

Influence of intramuscular fat content on lipid composition,sensory qualities and consumer acceptability of cured cooked ham

The present study is part of a project which aims to examine the influence of intramuscular fat (IMF) content on the sensory attributes and consumer acceptability of pork. An experiment was conducted to evaluate the influence of IMF level in muscle semimembranosus (SM) on the composition of its lipid fraction and on the sensory qualities and consumer acceptability of cured cooked hams. Thirty‐two carcasses were selected 24 h after slaughter from 125 Duroc × Landrace castrated male pigs showing large variability in SM muscle IMF content and were assigned to four IMF groups: ≤2%, 2–3%, 3–4% and >4%. Cured cooked ham slices were evaluated by a trained expert panel of 12 members and by a group of 56 consumers. Results from lipid analyses indicate that (i) an increase in IMF content was almost entirely reflected by an increase in the triglyceride content of the muscle and (ii) higher IMF levels were associated with higher free fatty acid and monoglyceride and lower cholesterol levels. The sensory evaluation of marbling significantly increased with IMF level, whereas other sensory qualities were unaffected. High IMF levels significantly depreciated the consumer perception of fat, aspect, taste and smell of ham slices. Overall, slices with the highest IMF levels got the least favourable rating by consumers. The present study indicates that increased IMF levels in muscle semimembranosus do not have significant effects on the sensory attributes of cured cooked hams, apart from the perception of marbling. However, high IMF levels have detrimental effects on the acceptability by consumers. © 2000 Society of Chemical Industry     

干腌火腿微生物研究进展

文章综述了干腌火腿微生物研究的现状,概述了酵母菌、霉菌、细菌对火腿中微生物多样性的影响,阐述了真菌毒素、致病菌及腐败菌等有害微生物对火腿安全性的影响,并对干腌火腿发酵产业的发展方向进行了展望。     

Effects of phosphine and methyl bromide fumigation on the volatile flavor profile and sensory quality of dry cured ham

In separate experiments, randomized complete block designs with three replications were utilized to evaluate the effects of phosphine (PH₃) (0, 200 and 1000 ppm for 48 h) and methyl bromide (MB) (0, 4, 8, 16, and 32 mg/L for 48 h) fumigation concentration on the volatile flavor compound concentrations in dry cured ham. Minimal differences existed (P > 0.05) in the presence and concentration of aroma active compounds in both PH₃ and MB fumigated hams but sulfur and oxidation compounds were more prevalent (P < 0.05) in the fumigated treatments when compared to the control. As phosphine fumigation concentration increased, the residual concentration of phosphine also increased in the hams (P < 0.05), but all samples contained levels that are lower than the legal limit of phosphine allowed in stored food products (0.01 ppm) in the United States. A triangle test (n = 56) indicated that consumers could not discriminate (P > 0.75) between the control hams and those that were fumigated with PH₃. Minimal aroma/flavor differences existed among MB, PH3 and control hams, and dry cured ham that was fumigated with PH₃ was safe for consumption based on residual phosphine concentrations in the meat tissue.     

Generation of volatile flavour compounds as affected by the chemical composition of different dry-cured ham sections

The composition of different dry-cured ham sections has been determined in order to study their contribution to flavour generation. The proteins, free amino acids, free fatty acids and volatile compounds were analysed in different sections of dry-cured hams with 7 and 12 months of processing. The main differences among sections were detected in free amino acids and volatile compounds. To sum up, there was a higher concentration of free amino acids in the centre section than in the butt and shank sections. However, after 12 m of ripening the difference among sections was lower and only the butt was the one with the lowest concentration. Similar results were obtained in the content of volatile compounds, the centre part of the hams contained a higher proportion of volatile compounds after 7 m of ripening than the shank and butt sections. Nevertheless, in 12 months dry-cured hams there are changes in the proportions of volatile compounds being the centre section the one with the highest proportion of volatile compounds due not only to the higher concentration of free amino acids but also to the oxidation of unsaturated fatty acids.     

Influence of PSE pork on the quality and economics of cooked, cured ham and fermented dry sausage manufacture

PSE pork (pH(1) ≤ 5·8) and non-PSE pork (5·8 < pH(1) ≤ 6·4) were used for cooked, cured ham production and, with beef, in a 48 52 mixture for fermented dry sausage manufacture. Technological yields of PSE and non-PSE hams were, respectively, 94·0 and 105·9% which corresponded to 5·0 FMK/kg loss for the PSE ham compared to the non-PSE ham. In addition, cooked PSE ham had a poorer organoleptic quality compared to non-PSE ham. However, PSE meat could be mixed with normal meat for the production of fermented dry sausage without quality defects.     

Influence of crossbreed on the degradation of myofibrillar proteins and on the cathepsin B+L activity in dry cured hams

Enzymatic activity and proteolysis undergone by the proteins extracted from the Semimembranosus and Biceps femoris muscles using high ionic strength buffer were studied in raw hams and hams dry-cured for 11 months. The hams were taken from three different crosses of three breeding enterprises: cross A (75% Duroc-25% Landrace); cross B (50% Duroc-25% Landrace-25% Large White); and cross C (75% Duroc-25% Landrace). Higher cathepsin B+L activity levels were recorded in the Semimembranosus muscle than in the Biceps femoris muscle both in the raw hams and in the cured hams, residual enzymatic activity in the hams being 1% of the initial levels; significant differences were not found for the three crosses. The proteolysis index was higher for the Biceps femoris muscle, similar values being observed for all three crosses. The raw hams from cross A exhibited the highest relative density values for low molecular mass peptides and the lowest relative density values for high molecular mass peptides. After 11 months of curing, proteolysis levels were highest in the hams from cross B, which had the highest levels of 8, 9 and 22 kDa fragments (in the Semimembranosus muscle) and 25 and 9 kDa fragments (in the Biceps femoris muscle). This finding confirms that genetic factors have an influence on proteolysis.     

Sex and crossbreed effects on the characteristics of dry-cured ham

The right ham of 76 gilts and 82 barrows from five different crossbreeds, which included Duroc (DU), Landrace (LR), Large White (LW) and Belgian Landrace (BL) lines - DUx(LRxLW), LWx(DUxLW), LWx(LRxLW), BLx(DUxLW) and BLx(LRxLW) - were processed to produce dry cured hams. The external appearance of the hams, and the colour and thickness of the subcutaneous fat were determined. Chemical analyses, appearance, texture, flavour and odour were evaluated on biceps femoris (BF) muscle. The hams from the barrows showed a thicker and whiter layer of subcutaneous fat, higher marbling and lower processing loss than those from gilts. Hams from DU-sired pigs presented the highest marbling, but the worst texture characteristics. The texture characteristics of the hams from the LBx(LRxLW) cross were similar to those of the hams from DU-sired pigs. The hams from the LW-sired crosses showed the highest intensity of holes surrounding the coxo-femoral joint. The LBx(DUxLW) could be the most suitable among the five crosses studied for dry-cured ham production under the processing conditions used in this study.     

Production and stability of patulin, ochratoxin A, citrinin, and cyclopiazonic acid on dry cured ham

Toxinogenic fungal species can be isolated from dry cured meat products, raising the problem of the direct contamination of these foods by mycotoxins known to be carcinogenic or potent carcinogens. Because the contamination of a food by mycotoxins can be considered a balance between production and degradation, the stability of mycotoxins on dry cured meat was also investigated. This study focused on patulin, ochratoxin A, citrinin, and cyclopiazonic acid that can be produced by fungal species previously isolated from dry cured meat products sold on the French market. We demonstrated that neither patulin nor ochratoxin A was produced on dry meat by toxigenic strains, whereas relatively high amounts of citrinin and cyclopiazonic acid were found after a 16-day incubation period at 20 degrees C (87 and 50 mg/kg, respectively). After direct contamination, the initial content of patulin rapidly decreased to become undetectable after only 6 h of incubation at 20 degrees C. For both citrinin and ochratoxin A, the kinetics of decrease at 20 degrees C was less rapid, and the two toxins presented half-lives of 6 and 120 h, respectively. By contrast, more than 80% of the initial contamination in cyclopiazonic acid was still found on ham after a 192-h incubation period. Toxin stability was not affected by storage at 4 degrees C. These results suggest that growth of toxigenic strains of Penicillium has to be avoided on dry meat products.     

Changes in the volatile flavour components of Jinhua ham during the traditional ageing process

Changes in volatile flavour compounds of Jinhua ham were studied during the traditional ageing process of sun‐drying, loft‐ageing and post‐ageing. About seventy‐eight volatile compounds were tentatively identified, including aldehydes (ten to fifteen), carboxylic acids (eight to ten), alcohols (thirteen to fourteen), ketones (eight), esters (eight to ten), aromatic hydrocarbons (five), sulphur‐containing compounds (two to three) and pyrazines (zero to four) which were the main components. The percentage content of aldehydes and ketones reduced over time as the temperature rose with each stage of the process, whereas alcohols, carboxylic acids, esters, pyrazines and sulphur‐containing compounds increased gradually. Jinhua ham's high‐temperature maturating process (40 days at 30–37 °C) made the percentage content of carboxylic acids arising from Maillard reactions markedly increase (from sun‐dried ham 8.45% to fully aged ham 19.23%). This was the most obvious difference between Jinhua ham and the Mediterranean dry‐cured hams, and was the main factor leading to the unique flavour of Jinhua ham.     

Effects of dietary high-oleic acid sunflower oil, copper and vitamin E levels on the fatty acid composition and the quality of dry cured Parma ham

The effects of seven isoenergetic dietary treatments: (1) no sunflower oil, 35 mg/kg Cu, without α-tocopheryl-acetate added; (2) to (7) 6% high oleic acid sunflower oil (HOSO), 35 or 175 mg/kg Cu crossed with a 0, 100 or 200 mg/kg α-tocopherol addition, were tested on quality characteristics of dry cured Parma hams from a total 84 Large White gilts. No statistically significant effect was detected on parameters of early evaluation of seasoning loss of hams. The seasoning loss and intramuscular fat content of seasoned hams averaged 28.1 and 3.3%, respectively, with no effect of the diet composition. The CIE L*a*b* colour values taken on the surface of the lean from Parma ham were not affected by dietary oil inclusion, nor by copper levels and by α-tocopherol addition in the feed mixture, except for the 'a' value that increased in HOSO groups (P<0.01) and in groups with α-tocopherol addition (P<0.01). The TBARS values in lean were reduced by the inclusion of HOSO (P<0.05) and α-tocopherol supplementation (P<0.10). Compared to the no oil group, the Parma hams in the HOSO groups showed a higher oleic acid content in the covering fat, but not different in neutral and polar fractions from semimenbranosus muscle. The oil inclusion reduced the saturated fatty acid content in subcutaneous fat and neutral lipids fraction from muscle to 30-34% No effect of α-tocopherol and copper levels were observed on fatty acids profiles. From the subjects fed the HOSO diet softer Parma hams were produced than those fed the control diet (χ(2)<0.05), while α-tocopherol and Cu levels did not influence the sensorial evaluation of hams. The inclusion of an oleic acid rich source in heavy pig diet brought about an improved nutritional value, but also the possible need of a prolonged ageing time to achieve an ideal firmness of Parma ham. Dietary α-tocopherol supplementation improved the red colour slightly and the lipid stability in Parma ham, while the supplementation of Cu in the diet had no influence on the tested parameters.     

涂膜制作低盐干腌火腿的研究

传统干腌火腿的盐分含量为9％～12％，生产周期长达6个月以上。本项研究，将新鲜猪腿肉去皮、去骨和切块后进行干腌，腌肉块经防腐涂膜处理后进行干燥和发酵，可以制成块状低盐干腌火腿，盐分含量降低到5％～7％，生产周期缩短到1个月，与传统工艺制作的干腌火腿的风味和口感相同。     

Relationship between flavour deterioration and the volatile compound profile of semi-ripened sausage

This study provides data on the relationship between flavour deterioration and the volatile compound profile of semi-ripened pork salami kept under retail conditions for up to 150 days. The flavour of salami deteriorated for 120 days, resulting in rancidity and a loss of acceptability. TBARS increased from 0.16 to 0.57 MDA/kg. The flavour changes during the shelf life of salami were monitored from changes in the volatile profile. The retailing time influenced (p < 0.05) the level of 27 of the 30 headspace volatiles determined by SPME–GC/MS. Flavour deterioration was associated with the loss and/or degradation of volatiles resulting from spices and microbial activities, and the formation of volatiles from lipid oxidation. The levels of 2-heptenal and methyl esters of heptanoic, pentanoic and hexanoic acids were the best discriminators of storage time, and therefore seem to be promising as marker compounds of flavour deterioration and acceptability.     

Authentication study of volatile flavour compounds composition in Slavonian traditional dry fermented salami “kulen”

Volatiles from famous Slavonian salami “kulen” were isolated by nitrogen purge and steam distillation (NPSD) and analysed, for the first time, by gas chromatography and mass spectrometry (GC and GC–MS). In all, 119 organic compounds were identified that originated from lipid oxidation, amino acid degradation, smoke treatment and added spices with different distribution among NPSD traps. NPSD method enabled comprehensive profiling with almost exclusive distribution of several major compounds in particular trap with little or no interference from abundant lipid constituents in the samples. The major identified flavour important compounds were methylphenols, methoxyphenols, organosulphur compounds (diallyl sulphide, diallyl disulphide, methylallyl disulphide, diallyl trisulphide and methional) and several derivatives of 2-cyclopenten-1-one such as ethyl cyclotene. Non-important flavour constituents such as high-molecular fatty acids, alcohols and aldehydes were also present among abundant compounds. General trends for possible discrimination of “kulen” volatiles profile among other European salami volatiles were noticed (lower amount of terpenes and higher percentages of diallyl sulphide, methoxyphenols, methylphenols and 2-cyclopenten-1-one derivatives).     

植物乳杆菌SP-3对干酪挥发性风味物质的影响

采用固相微萃取(SPME)富集干酪模型中的挥发性风味物质,并以气相色谱—质谱联用仪(GC-MS)检测结果来评定植物乳杆菌SP-3对干酪挥发性风味的影响。结果表明,添加植物乳杆菌SP-3的干酪模型中2-丁酮、2-甲基-1-丙醇、3-甲基-1-丁醇、1-戊醇、4-甲基-2-戊醇、3-羟基-2-丁酮等具有坚果味、水果味和奶油味的物质明显高于对照组,而二甲基硫化物和3-甲硫基-1-丙醇的浓度则低于对照组。     

Impact of shoot maturity on chlorophyll content,composition of volatile flavour compounds and plain black tea chemical quality parameters of clonal leaf

Chlorophyll content in black teas increased with a rise in shoot maturity, while the theaflavin content, brightness, total colour and tasters' evaluation declined. The increase in chlorophyll content in black teas of clone S15/10 was in a parallel with the greenness of the black tea infusions as described by tea tasters. These relationships were less pronounced in clone 6/8. The thearubigins content and the individual volatile flavour compounds composition in black tea remained the same or declined with increased shoot maturity, respectively. The results suggest that the impact of chlorophyll content on black tea quality is clonal dependent and becomes more significant as tea shoots mature and when the other plain black tea chemical quality parameters are low.     

A study of volatile flavour substances in Dalmatian traditional smoked ham: Impact of dry-curing and frying

Volatiles from Dalmatian prosciutto were isolated by solvent extraction (SE), simultaneous distillation extraction (SDE) and nitrogen purge and steam distillation (NPSD) and analyzed by GC and GC–MS. In all, 46 compounds were identified by SDE and SE (including fatty acids, aldehydes, phenols, esters, ketones and others), while 81 compounds were identified by NPSD (headspace volatiles including phenols, aldehydes, hydrocarbons, ketones, alcohols, esters and heterocyclic compounds). Regarding the impact of dry curing period on the volatiles, an increase in the percentages of aldehydes and esters during the ripening of the prosciutto was observed. Quantitative percentage differences among fried and raw samples were particularly evident in respect of aldehydes (SDE and SE). The NPSD method provided additional information of the volatiles from fried ham, since the pyrazines and most of the lower aldehydes that are important thermally derived flavour compounds were only isolated by NPSD (not by SE and SDE).     

Influence of pH and ethanol on malolactic fermentation and volatile aroma compound composition in white wines

The present study investigated the influences of pH and ethanol on malolactic fermentation (MLF) and the volatile aroma profile of the subsequent white wines from Riesling and Chardonnay inoculated with two different Oenococcus oeni strains. In all cases MLF was induced after completion of alcoholic fermentation (AF). Partial MLF occurred under low pH 3.2 and high alcohol (118.3 g/L) conditions. In the cases with complete MLF, the time required for each strain varied from 13 to 61 days and was dependent on bacterial culture, cultivar and wine parameter. Chemical properties of each wine were determined after AF, complete and partial MLF. The wines showed significant differences in total higher alcohols, esters and acids that are important for the sensory profile and quality of wine. This work demonstrated that the wine matrix as well as the pH and alcohol concentration affects MLF and the final volatile aroma profile. Results indicate that changes in volatile aroma composition are not necessarily related to complete MLF and that partial MLF already has distinct influences on the wine aroma profile of white wines.     

Effect of intensity of smoking treatment on the free amino acids and biogenic amines occurrence in dry cured ham

The aim of this study was to investigate the effect of the intensity of smoking treatment on the evolution of free amino acids (FAAs) of dry cured ham during processing. The correlation between FAAs and biogenic amines (BAs) content was also investigated as well as its effect on the perception of some sensory characteristics of ripened hams.