知母对甲亢小鼠模型心肌β受体更新速率的下调作用

采用(-)~(125)I-心得静为特异配基的β受体结合分析研究表明,知母可抑制甲亢小鼠模型心肌β受体数的增加。用BAlpM不可逆阻断已存在的β受体后,测定不同时间间隔心肌细胞膜β受体数,计算出各动力学参数。知母可使甲亢小鼠心肌β受体生成速率降低48％;降解速率常数降低35％。知母对生成的抑制超过对降解的抑制,结果使甲亢模型的β受体数显著降低,接近正常值。     

~(125)I-心得静的标记及β-肾上腺素能受体的结合试验

本文报道了用生物作用最强的β-受体阻断剂(一)心得静作配基,用~(125)I标记制备高亲和力和高比活性的放射配基。     

^125I—雌二醇？6—肟—碘代组胺的制备

文章介绍了以17β-雌二醇(17β-E_2)为底物,合成制备雌二醇-6-肟-组胺,用~(125)I碘化后,经一步硅胶GF_(254)薄板层析(TLC)分离,即可得到~(125)I-雌二醇-6-肟-碘代组胺的方法。用自身置换法测定比活度约74MBq/μg。并对其免疫活性进行了实验。与过量抗体(10倍)结合率平均82.5%(n=3)。认为是建立17β-雌二醇放射免疫分析方法比较理想的试剂。     

用~(125)I-β-CIT与~(125)I-IBZM体内放射自显影研究MPTP致帕金森病小鼠中枢多巴胺转运蛋白与受体变化

应用12 5I- β -CIT和12 5I-IBZM体内放射自显影技术研究了MPTP致帕金森病小鼠中枢多巴胺转运蛋白 (DAT)与多巴胺D2 受体 (D2 R)变化。结果表明 :对于给予MPTP ( 30mg kg·d)日数分别为 1、3、5、7d的小鼠 ,用药早期 ,DAT密度即表现为降低 ,而D2 R密度在早期未有显著改变。随着日数增加 ,D2 R密度表现为升高 ,受体呈超敏状态。提示联合应用DAT与D2 R显像剂同时进行SPECT显像 ,有利于临床PD病诊断与鉴别诊断以及评估黑质 -纹状体DA神经元的变性程度。     

125I-4-(N-苄基哌啶基)-4-碘代苯磺酰胺的体内σ受体亲和性能研究

经化学合成与放射性碘取代三丁基锡法制得^125I-4-(N-苄基哌啶基)-4-碘代苯磺酰胺，并观察了其在荷不同肿瘤细胞小鼠体内的分布，以探讨^125I-4-(N-苄基哌啶基)-4-碘代苯磺酰胺的体内σ受体亲和性能及应用于σ受体阳性肿瘤导向诊断与导向治疗的可能性。结果显示：^125I-4-(N-苄基哌啶基)-4-碘代苯磺酰胺在表达σ受体的脏器——肝脏、脾脏、心脏、肺脏、肾脏、小肠以及小鼠肝癌H22与乳腺癌EAC中具有高的摄取；σ受体配体氟哌啶醇可抑制标记配体在心脏、肺脏和脾脏中的摄取，但在肿瘤EAC中的摄取未受抑制。肿瘤与脑和肌肉有较高的T／NT值，肿瘤与肌肉的T／NT值在注射后2h最高，可达6．98。     

~(123)I-邻碘马尿酸的快速标记及生物试验

~(123)I-邻碘马尿酸(下称~(123)I-OIH)由于(123)I半衰期短、γ射线能量合适、无β射线发射,较之常用的~(131)I-OIH,不仅可使病人接受剂量显著减小,而且成像清晰、可提高诊断的准确性,是目前较理想的肾动态显像剂。 Ellas用熔融法标记~(131)I-间碘马尿酸之后,Westera等制备了~(123)I-OIH。本文旨在以我们自制的~(123)I-碘化钠和OIH为原料,找到一个操作简便、产率高,放化纯度好、工艺稳定、实用性强的快速标记~(123)I-OIH的方法。并进行小鼠体内分布试验以及兔和犬的肾动态γ照相。     

β-肾上腺素受体放射配基分析法的条件探讨

本文对β-肾上腺素受体放射配基分析法中的缓冲液、膜受体制备条件、胰蛋白用量、结合与游离配基的分离方法及非特异性结合的控制等方面作了探讨和改进,使方法的稳定性和可靠性有了明显的提高,并对所建方法进行了较完整的考核。     

125I示踪研究β-NGF对血脑屏障的通透性

从胎儿脑中提取β-NGF,采用透析和离子交换纤维素层析法分离得到β-NGF提取物,并用SDS-PAGE电泳测得β-NGF相对分子量为13 kD,用等电聚焦电泳测得β-NGF等电点分别为9.0、9.2和9.3;通过对PC12细胞培养观察到β-NGF可促进PC12细胞的生长.动物实验结果表明,静脉注入125I-β-NGF后15 min时,有(2.41±0.12)%的摄入量可透过血脑屏障;30 min时,进入脑组织中的125I-β-NGF即可增至(4.20±0.07)%.这为开展β-NGF的临床治疗应用提供了科学依据.     

间碘苄胍的标记和动物体内分布

自八十年代起,国内外学者对心肌显象的定位诊断造影剂研究众多,希望寻找一种显象清晰、成本低的药物以代替费用昂贵的~()Tl。其中有正肾上腺类研究,一类是间-~(131)I-碘苄胍为主,此类制剂可作为肾上腺髓质的定位诊断和治疗药物,一类是β-肾上腺素激导性的对抗物。我们自1982年2月开始进行β受体类药物的研究,并于1983年11月开始将间-~(131)I-碘节胍(M~(131)IBG)制剂应用于临床。本文介绍一种~(131)碘标记新技术及动物实验的结果。     

β-CIT的131I标记及其初步临床应用

用过氧乙酸法进行了β-CIT的131I标记,并用标记物对4例正常对照、8例帕金森氏病(PD)患者、3例帕金森氏综合征(PS)患者进行显像,计算纹状体与小脑的放射性摄取比(特异性摄取)及纹状体131I-β-CIT摄取的非对称指数AI.结果显示131I-β-CIT放化纯度达(97.6±0.3)%,室温下放置4 h以及分别与水、人新鲜血清孵育4 h后,其放化纯度仍均＞95%;纹状体能特异摄取131I-β-CIT,与正常对照组及PS组相比,PD患者双侧纹状体摄取的放射性明显降低(P＜0.01),且症状对侧降低更明显;PS患者与正常对照组相比,其纹状体摄取无明显差异(P＞0.05).直线回归分析显示,PD患者症状的严重程度与纹状体特异摄取131I-β-CIT下降密切相关.提示131I-β-CIT多巴胺转运蛋白显像可作为PD诊断、鉴别诊断和病情严重程度的客观指标.     

老年大鼠脑M1胆碱受体分布的放射自显影研究

采用放射自显影技术观察自然衰老大鼠几个重要脑区Ｍ１受体密度的变化及知母或其甘元ＺＭＳ的调整作用。图像分析仪给出不同脑区的平均灰度，老年鼠皮层、海马、纹状体的灰度显著低于青年鼠，皮层和纹状体降低１５％左右，海马降低不到１０％。给开始进入老年期的大鼠长期口服知母或其甙元ＺＭＳ，三个脑区的Ｍ１受体密度均明显高于老年对照组。     

^125I—抗Tg—McAb与甲状腺癌细胞结合的体外实验研究

选用自制的１８Ａ１和１８Ａ１１两株ＭｃＡｂ进行体外活细胞结合实验,定量观察＾１２５Ｉ标记的１８Ａ１和１８Ａ１１与正常甲状腺组织细胞及某些肿瘤细胞膜结合的反应性。实验结果显示,＾１２５Ｉ－抗Ｔｇ－ＭｃＡｂ与高分化甲癌细胞的结合率均〉３０％;与髓样癌结合率为８％－１２％;与正常甲状腺和甲状腺良性瘤细胞结合率〈１０％;其他细胞均〈４％。碘标记ＭｃＡｂ与正常甲状腺和甲状腺良性瘤细胞的结合率显著低于高分化甲     

Optimised plasma stabilisation at TEXTOR with an advanced, real-time digital control scheme

The tokamak TEXTOR at the Research Centre in Jülich is in operation since more than 25 years. The various control systems at the start, in 1982, were based on analogue techniques, a standard at the time, and were later partly replaced by specially developed digital systems. These systems proved their robustness over the years. As a replacement for the old system, off-the-shelf products were used to ensure continuity, reliability and to reduce the development cost. To provide advanced control scenarios, the new system allows the implementation of more sophisticated algorithms for magnetic and kinetic control. The LabVIEW Real-Time (RT) modules and real-time hardware from National Instruments satisfy these requirements to a large extent. The new system has already been successfully commissioned at TEXTOR and is used to calculate in real-time the plasma density profile (10 ms), the Shafranov shift (10 ms), the plasma vertical and horizontal position (20 $\mu$s) and to control the plasma shape (1 ms). TEXTOR has circular plasmas and has an iron core. Its central part is operated in saturation. During the saturation phase, stray fields change the plasma shape from nearly circular to slightly triangular. By using a shape-control coil set, we can control and adjust the plasma form. The new real-time system is presented as well as the implemented control applications.     

861消瘤片对子宫肌瘤患者血浆性激素及肌瘤组织受体含量的影响

用中药861消瘤片治疗子宫肌瘤患者50例(子宫体包括肌瘤体积不超过三个月妊娠子宫大小),六个月后,子宫肌瘤消失率为26％,缩小率为46％。对22例肌瘤患者于治疗前后测定了血浆中FSH、LH、E_2、P、T含量,发现治疗前患者P含量明显低于正常妇女(p<0.05)。治疗后分泌期P含量升高(p<0.05)。对30例肌瘤组织中EcR、EnR含量进行了测定,中药组与对照组比较,EcR、EnR含量均明显降低(p<0.05)。临床和实验研究提示:该药治疗机理可能与调节内分泌功能及抑制肌瘤组织中ER水平有关。     

M2受体cDNA转染CHOm2细胞的某些药理学和生物学特性

转染ｍ１、ｍ２和ｍ４ ｃＤＮＡ的ＣＨＯ细胞膜受体与＾３Ｈ－ＱＮＢ结合,能给出典型的的饱和曲线,Ｈｉｌｌ系数接近１。单位点竞相结合表明,对ＰＺＰ抑制能力为ＣＨＯｍ１〉ＣＨＯｍ４〉ＣＨＯｍ２,对ＭＥＴＨ抑制强度为ＣＨＯｍ２〉ＣＨＯｍ４〉ＣＨＯｍ１。与药理学ＰＺＰ对Ｍ１受体的亲和力最高和ＭＥＴＨ对Ｍ２的亲和力最高是一致的。ＣＨＯｍ２对Ｆｏｒｓｋｏｌｉｎ和Ｃａｒｂａｃｈｏｌ影响ｃＡＭＰ的结果,证明该Ｍ受体     

The effect of adrenergic receptor—adenyl cyclase system on myocardial ischemic preconditioning in rats

In order to study the effects of every part of adrenergic reccptor-adenyl cyclase system on ischemic preconditioning of myocardium in rats in vivo, SD rats were divided into three groups: IP group, I/R group and CON group. Rats were received surgical procedure and undergone left coronary artery occlusion and reperfu-sion. Hearts were extracted to analyze the infarct size by TTC staining, to measure serum myocardial enzymes, to study β-AR Bmax and Kd by radioligand binding assay of receptors (RAB), and to check the activity of AC and the content of cAMP by ra-dioimmunoassay (RIA). The infarct area was found much smaller in IP group than I/R group (p <0.001); CK,CK-MB and LDH were found significantly higher in I/R group (p <0.001). The Bmax of β-AR in IP group were higher than in I/R group (p <0.001). No difference of Kd could be seen between IP and I/R group. In IP group, the activity of AC and the content of cAMP were higher than I/R group (p <0.05 and 0.001, respectively). It is concluded that ischemi     

Investigation of the Flow Structure on a Flat Plate Induced by Unsteady Plasma Actuation with DNS Methods

An investigation into the flow characteristic on a flat plate induced by an unsteady plasma was conducted with the methods of direct numerical simulations(DNS).A simplified model of dielectric barrier discharge(DBD) plasma was applied and its parameters were calibrated with the experimental results.In the simulations,effects of the actuation frequency on the flow were examined.The instantaneous flow parameters were also drawn to serve as a detailed study on the behavior when the plasma actuator was applied to the flow.The result shows that induced by the unsteady actuation,a series of vortex pairs which showed dipole formation and periodicity distribution were formed in the boundary layer.The production of these vortex pairs indicated a strong energy exchange between the main flow and the boundary layer.They moved downstream under the action of the free stream and decayed under the influence of the fluid viscosity.The distance of the neighboring vortices was found to be determined by the actuation frequency.Interaction of the neighboring vortices would be ignored when the actuation frequency was too small to make a difference.     

辐射诱导细胞内凝溶胶蛋白变化及其对小鼠氧化损伤的影响

为研究凝溶胶蛋白(Gelsolin)在辐射损伤中的作用,以6、8和12Gyγ射线分别照射BALB/c小鼠和人小肠上皮隐窝细胞(HIEC).于照后不同时间抽提HIEC全蛋白,Western blot半定量法检测胞浆型Gelsolin(cGSN)蛋白相对含量.照射后24h给受照小鼠注射重组人源Gelsolin蛋白,不同照后...     

Recent Results concerning Interplanetary Plasma

The MIT plasma probe, installed aboard the first IMP, has shown that the space around the earth is sharply divided into three regions. At large distances from the earth, we find a region (region 1) occupied by a plasma moving with a velocity from about 300 to 400 km/sec, in a direction nearly radial away from the sun; its thermal energy density is small compared with its kinetic energy density of bulk motion. Presumably we see in this region the "solar wind," unperturbed by the presence of the earth. As we approach the earth, we pass from region 1 to region 2 where the velocity of the plasma is often below the limit of detectability and in any case much smaller than in region 1. The thermal energy density is large, corresponding to temperatures of the order of millions of degrees, but a rigorous definition of the temperature is impossible because the velocity distribution does not appear to be Maxwellian. Approaching the earth further, we meet a second boundary, separating region 2 from region 3, where the plasma probe does not detect any plasma. Both boundaries have a characteristic comet-like shape, with a blunt nose facing the sun and a long diverging tail in the opposite direction. Presumably the boundary between regions 1 and 2 represents a shock front, across which the plasma flow changes from supersonic to subsonic.