Nutritional value of broad bean seeds. Part 1: Starch and fibre

This paper characterizes starch and dietary fibre in the seeds of fine-size and large-size seed varieties of broad bean and in edible varieties of pea for comparison. The experimental material included seeds at full physiological maturity. The fine-size seed varieties of broad bean, Gobik and Goral, contained slightly less total starch (62.32% and 62.19% of dry matter) than the large-size seed varieties, Windsor Bialy and Bartom (65.32% and 65.26% of dry matter). It was shown that fine-size seed varieties of broad bean are a good source of resistant starch, which is comparable to large-size seed varieties. As far as the content of total starch is concerned, the share of this form of starch in broad bean seeds amounted to 1/3. Digestible starch in broad beans was in the range of 39-42% of dry matter, pea contained approximately one half less starch of this type. Moreover, it was found that the relation of rapidly digestible starch to slowly digestible starch amounted to 1:1 irrespective of broad bean variety. Dietary fibre in broad beans was in the range of 20.36%-26.79% of dry matter while half of it was found to be concentrated in the seed coat. The soluble fraction of the total content of fibre amounted to 11.81% in the Bartom variety and up to 15.89% in the Gobik variety.     

Nutritional value of broad bean seeds. Part 2: Selected biologically active components

Selected biologically active non-nutrient components (BANS) were determined in broad bean seeds of fine-size and large-size seed varieties and in pea seeds for comparison. Among the analysed biologically active substances the broad bean seeds of fine-size and large-size seed varieties were differentiated mainly by phenolic compounds (including flavanols and proantocyanidines) which appear in twice as large quantities in large-size seed varieties. It was shown that in comparison to pea, broad bean seeds are characterised by a higher content of phenolic compounds, phytates as well as a higher activity of inhibitors of trypsin and amylases. Moreover, it was found that phenolic compounds accumulate mainly in the dark-colored seed coats of large-size broad bean and this fact is related to higher activity inhibiting-amylases of methanol extracts from this fraction of seeds.     

Preparation of broad bean (Vicia faba L. minor) products Part 3. Pretreatment of broad beans and broad bean flour —processes and effects

For improving sensory, functional and other properties broad beans are pretreated in 3 different ways. The pretreatments include defatting or isoelectric washing of broad bean flour and hydrothermal treatment of whole dehulled broad beans. Every pretreatment process leads to particular advantages, described in this paper, although sensory improvements are found in all pretreated products.     

Rapeseed: constituents and protein products. Part 3. Nutritional value of protein-enriched products

The review concerns the nutritional properties of protein preparations from Brassica seed with different species of animals. Special consideration is taken on the one side to the effect of feeding varieties with high and low glucosinolate content, on the other hand to the influence of technological treatment of this products.     

Dietary fibre in bread and corresponding flours —Formation of resistant starch during baking

Dietary fibre, assayed with an enzymatic/gravimetric method, was higher in wheat/rye bread than in the corresponding flours. The increase was most pronounced in crumbs from bread baked with mainly low-extraction-rate flour, and could be accounted for to a large extent as “resistant starch”, i. e. a starch fraction available to amyloglucosidase only after solubilization with 2m-KOH. The resistant starch was formed at dough-making and/or baking and did not increase further during freezing or storage at room temperature. The chemical modifications leading to resistant starch formation remain to be investigated. Starch-lipid complexes are probably not involved, since these are hydrolyzed by the heat-stable amylase used in the dietary fibre assay.     

Effect of processing on the composition of dietary fibre and starch in some legumes

The effect of processing on the total dietary fibre (TDF) insoluble (IDF) and water-soluble (SDF) fractions as well as total (TS), available (AS) and resistant (RS) starch were studied in three legumes, viz. bengalgram (Cicer arietinum L.), Cowpea (Vigna unguiculata) and greengram (Vigna radiata). The processes studied were fermentation, germination, pressure-cooking and roasting. The dietary fibre (DF) content and its components were determined using the enzymatic-gravimetric method. The TS content was determined by the enzymatic method after solubilization with KOH. The DF content ranged from 23.2 to 25.6 g/100 g in the raw and 16.0 to 31.5 g/100 g in the processed legumes. All the processing treatments significantly decreased the SDF content and increased the IDF content of all the three legumes. The mean TS, AS and RS content of the raw legumes were similar, 46.9, 36.7 and 10.2 g/100 g respectively. AS content of all the legumes was reduced by the processing treatments, except pressure cooking. Correspondingly, higher amounts of RS were observed in the processed legumes, except pressure cooked, resulting in an increase in the TDF content.     

Chemical composition,dietary fibre and resistant starch contents of raw and cooked pea,common bean,chickpea and lentil legumes

The chemical composition and the contents of resistant starch and soluble and insoluble dietary fibre of pea (Pisum sativum L.), common bean (Phaseolus vulgaris L.), chickpea (Cicer aretinum L.) and lentil (Lens culinaris Med.) legumes, were studied. Raw and freeze-dried cooked samples were used, both in the form of flour. Protein values of the legumes ranged from 18.5 to 21.9 g/100 g for the raw grains and from 21.3 to 23.7 g/100 g for freeze-dried cooked legumes. Chickpea stood out for the highest lipid content (p < 0.05), the lowest insoluble fibre values, and soluble dietary fibre not detected. The average content of resistant starch found in the legumes did not differ statistically (p > 0.05), being 2.23 ± 0.24 g/100 g for freeze-dried cooked legumes, and showing a slight reduction in comparison to the raw form.     

Effects of flour storage and heat generated during milling on starch,dietary fibre and polyphenols in stoneground flours from two durum‐type wheats

In the perspective of cereal‐based functional food development, the research compared stone watermill and stone mill which differed in the heating generated during grinding (30 and 60 °C) and the effects of storage on the preservation of phytochemicals. After 6‐month storage of flour, a decrease in soluble dietary fibre (loss of 1.9–2.5 g 100 g^?1) and bound polyphenols (loss of 6.2–8.5%) was observed, while other wheat grain components remained unvaried. Comparing the milling techniques, stoneground wheat grains (60 °C) showed the highest amylose (34.7–36.9% of total starch) and resistant starch amounts (0.85–1.16% of total starch), which could be of interest for lowering Glycaemic Index. Stoneground KAMUT^® starch had higher tendency to be converted into resistant starch and it also showed the higher degree of starch damage, as compared to the durum wheat variety Claudio. The study provided suggestions for modulating the production chain with the aim of supplying added value to wheat‐based products.     

Efficiency of some technological processes on reducing the residues of malathion and pirimiphos methyl in mature broad bean seeds

Residue study was performed on several insecticides which could contaminate local Egyptian beans. The effect of storage periods and various processing steps on lowering the residues of malathion and pirimiphos methyl in treated seeds and their processed products were investigated. The data indicated that malathion and pirimiphos methyl persisted for more than 90 days on and in stored mature dry broad beans after postharvest treatment. However, stored broad beans could be safely used for human consumption after 90 days when the insecticide residues reached safe levels. Washing removed 69 and 75% of malathion and pirimiphos methyl residues of treated broad beans, respectively. Malathion residue was not detected in various processed products. More than 89 and 99% of malathion residues were absent in dehulled and heated dehulled broad beans. In addition, pirimiphos methyl residues were reduced to 92, 97, 87, 99, 99, and 95% from the initial levels in treated beans following dehulling, cooking of dehulled beans, germination, cooking of germinated beans and cooking of the beans by the common method and under pressure, respectively.     

Enzyme activities,dietary fibre components and rheological properties of wholemeal flours from rye cultivars grown in Finland

Total and soluble dietary fibre, total and soluble pentosan and β‐glucan contents, activities of α‐amylase (EC 3.2.1.1) and endo‐β‐xylanase (EC 3.2.1.8) and viscous properties of aqueous suspensions of wholemeal flours during heating were determined in nine winter rye cultivars (Secale cereale L) grown in Finland in 1998–2001. There was marked annual and varietal differences in grain quality. In the rainy summer 1998 the yield was low, grains were small and dietary fibre content of the grains was high. Xylanase activity of the grains was high, which corresponded to the high content of soluble pentosans. In the dry summer of 1999, the pentosan content of the grains was low and β‐glucan content high. The effect of weather conditions and cultivar were also apparent in the differences in falling numbers, amylogram and swelling curve results. The two hybrid rye cultivars Esprit and Picasso had consistently highest falling numbers and amylogram peak viscosities. The activities of α‐amylase and xylanase had a moderate positive correlation with total pentosan content and the content of soluble pentosan. Xylanase activity had better correlation with the viscous properties of flour–water suspensions than α‐amylase. Surprisingly, α‐amylase activity had only a moderate negative correlation with falling number. Copyright © 2005 Society of Chemical Industry     

Effects of dietary fibre and soybean oil on the digestion of extruded and roller-dried maize starch

The effects of soybean dietary fibre (SDF), microcrystalline cellulose (MCC), konjac glucomannan (KGM), soluble soybean polysaccharide (SSPS) and soybean oil (SO) on in vitro digestion of extruded maize starch (EMS) and roller-dried maize starch (RDMS) samples were investigated. Adding MCC, particularly in high doses, induced slow digestion in EMS and RDMS samples, but not after adding SDF. KGM and SSPS addition reduced starch hydrolysis of RDMS but did not have a significant effect on EMS, whereas SO addition reduced starch hydrolysis of EMS but had no effect on RDMS. Principal component analysis demonstrated that the addition of MCC, SO, KGM (0.51%), and SSPS (0.51%) to EMS and KGM (0.51%) to RDMS were the best choices to slow the hydrolysis of starch, compared with that of other additives. These findings provide useful information on the selection of exogenous additives in the development of low glycaemic index products for maize starch.     

Studies on dietary fibre. A method for the analysis and chemical characterisation of total dietary fibre

A procedure for the analysis of total dietary fibre (including non-starchy polysaccharides and Klason lignin) is described. The method consists of extraction of homogenised and/or milled foodstuffs with 80% ethanol and chloroform, and analysis of acidic and neutral polysaccharide constituents, starch and Klason lignin in the residue. Uronic acid constituents were determined by a decarboxylation method, neutral sugar constituents as alditol acetates by a gas-liquid chromatographic method, starch by an enzymic method, and Klason lignin gravimetrically. The sum of anhydrosugars, anhydrouronic acids and Klason lignin from which the starch content is subtracted provides a measure of the total dietary fibre. The method described is reproducible, fairly rapid and gives informative quantitative characterisation of the fibre constituents.     

Soybean seeds and its by-product okara as sources of dietary fibre. Measurement by AOAC and Englyst methods

The composition of soybean seeds and its by-product okara has been studied in this work. Dietary fibre was analysed by Englyst et al. method, by enzymatic–gravimetric methods of AOAC and by the quantification of the monomers obtained from the AOAC residues after acid hydrolysis (AOAC plus hydrolysis). Total dietary fibre by the enzymatic–gravimetric methods of AOAC in okara (55.48 g/100 g dry matter) is more than twice that of soybean seeds (24.37 g/100 g dry matter). The proportion IF/SF is 11 in okara and 6 in soybean seeds. Dietary fibre results from enzymatic–gravimetric AOAC methods are higher in okara and soybean seed samples than those from the Englyst method (okara: 41.14 g/100 g dry matter; soybean seeds: 15.05 g/100 g dry matter), and AOAC plus hydrolysis (okara: 44.91 g/100 g dry matter; soybean seeds: 16.38 g/100 g dry matter). In the case of the insoluble fibre from both samples, AOAC plus hydrolysis gives significantly (p < 0.001) higher values than the Englyst method, whilst for soluble fibre the opposite occurs (p < 0.001). The main monomers in soybean seeds and okara fibre are glucose, galactose, uronic acids, arabinose and xylose. The proportion of each monomer is similar in soybean seeds and okara, so the healthy properties of soybean seeds fibre are also claimed for okara.     

Effect of irradiation,with and without cooking of maize and kidney bean flours,on porridge viscosity and in vitro starch digestibility

African traditional weaning foods are usually prepared from starchy flours at a total solids content of 50–100 g l^?1, giving a viscosity of 1000–3000 cP which is consumable by infants. These porridges have very low energy and nutrient densities. Processing to reduce the porridge viscosity and improve the energy and nutrient density may help in solving this problem. Maize, kidney bean and their 70:30 composite flours were irradiated at 0–10 kGy at room temperature. Irradiation greatly reduced the viscosity of porridges, probably by starch depolymerisation. Irradiation improved the starch digestibility of raw bean flours, possibly either by inhibiting α‐amylase inhibitors or by weakening the thick cell walls that enclose the starch granules, thus improving accessibility to enzymes. However, in raw maize, cooked maize and cooked bean flours there was maximum starch digestibility at a dose of 2.5 kGy, followed by small but significant decreases in starch digestibility at higher doses, more so in maize than in bean flours. The decrease in digestibility could be due to the formation of inhibitors of amylolytic enzymes (Maillard reactions products) or to the formation of resistant starch. Irradiation doses required to increase the total solids content of traditional maize‐ and/or bean‐based weaning porridges to at least 200 g l^?1 do not compromise starch digestibility significantly. © 2001 Society of Chemical Industry     

Preparation of broad bean (Vicia faba L. minor) products. Part 2. Broad bean protein isolates from seed flour in the pH range below the isoelectric point

Broad bean protein isolates in aqueous solutions form stable gels after heating at pH values below the isoelectric point (pH 2-3) if they are extracted at the same pH. But the acid extraction is a time-consuming and expensive process. This paper outlines a new and simple procedure for the preparation of broad bean protein isolates in the acid pH range. It consists of a short extraction of seed flour at pH 7.5 (30 min) followed by an after-treatment at pH 2-3 (60 min) and gives the highest yield of acid treated broad bean protein isolates after a sudden acidification.     

Preparation of broad bean (Vicia faba L. minor) products Part,I. Broad bean protein isolates from seed flour and their acetylation in the neutral pH range

It is possible to prepare broad bean protein isolates with a high yield directly from seed flour using a wet process. This process involves an aqueous extraction at pH 7.5 followed by separation of the insoluble residue and isoelectric precipitation of the protein from the aqueous extract. Following the same principle is it possible to prepare acetylated broad bean protein isolates with any adjustable acetylation degree. For that acetic anhydride is to be added and the adjusted pH is to be maintained during the extraction. In both cases the yield of the protein is mainly determined by the used solid-liquid ratio.     

Improvement of the AOAC 2009.01 total dietary fibre method for bread and other high starch containing matrices

The dietary fibre (DF) content in wheat grain based food products have been established with both the classical AOAC 985.29 dietary fibre and the new AOAC 2009.01 total dietary fibre protocol. There is a good agreement between the high molecular weight dietary fibre (HMWDF) contents measured with the AOAC 2009.01 method and (DF) content measured with the classical AOAC 985.29 method in wheat grain based food products. With the AOAC 2009.01 method also a significant amount of low molar weight dietary fibre (LMWDF), ranging from 1% to 3% w/w, was measured which is not quantified with the AOAC 985.29 method.