RP-HPLC法测定刺梨中的Vc含量

[目的]建立刺梨中Vc含量的测定方法.[方法]采用反相高效液相色谱法对刺梨中的Vc进行含量测定.[结果]维生素C在0.112～1.120 mg/ml范围内线性关系良好,平均回收率为99.29%.[结论]RP-HPLC法简便、快速、重复性好,可作为刺梨的质量控制方法.     

RP-HPLC法测定当归片中阿魏酸的含量

[目的]建立当归片中阿魏酸含量的RP-HPLC测定方法.[方法]采用反相高效液相法,以阿魏酸对照品作为内部标准品测定自制当归片中阿魏酸的含量.[结果]阿魏酸在8.3～41.6μg/ml范围内呈现良好线性关系,加样回收率为99.9%,RSD=0.51%(n=6).[结论]高效液相法操作简单、准确、重复性好、精密度高,可作为当归片的质量控制方法.     

竹壳葫芦茶的质量标准研究

[目的]建立竹壳葫芦茶质量标准.[方法]采用薄层色谱法(TLC)对竹壳葫芦茶中的救必应进行定性鉴别;采用高效液相色谱法(HPLC)测定竹壳葫芦茶中的槲皮素含量.[结果]TLC结果显示,竹壳葫芦茶在与救必应对照药材相应位置上,呈相同颜色的斑点,阴性对照无干扰.竹壳葫芦茶槲皮素含量为41.03～49.06 μg/包.[结论]该研究建立的定性及定量方法简单可行、重复性好,能有效地控制竹壳葫芦茶的质量.     

RP-HPLC法测定辅酶Q10含量及其透光率的日变化分析

[目的]建立高效液相色谱法测定辅酶Q10的含量.[方法]采用反相高效液相色谱法(RP-HPLC)洲定辅酶Q10的含量,并采用紫外分光光度计法对其透光率的日变化进行了分析.[结果]辅酶Q10在RP-HPLC操作条件下分离度良好.在40～300 μg/ml范围内与峰面积线性关系良好(r=0.999 9);最低检测限为0.4 ng;平均回收率为97.44%(n=3).RP-HPLC方法的系统适应性良好,回收率和精密度均满足分析要求.[结论]该方法简便、准确、重现性好,可用于辅酶Q10的质量控制,但应注意避光操作.     

HPLC法测定六味地黄丸熊果酸的含量

[目的]用高效液相色谱(HPLC)法测定六味地黄丸(浓缩丸)的熊果酸含量,提高熊果酸的质量控制标准.[方法]色谱柱:Welch Materials AQ-C18柱(5 μm,4.6 mm×250.0 mm);流动相:乙腈-水-磷酸(V:V:V=90:10:0.1);柱温:25 ℃;流速:1.0 ml/min;检测波长为210 nm.[结果]在0.457 6～1.542 3 μg/ml的线性范围内,熊果酸与峰面积呈良好的线性关系,平均回收率为99.31%,RSD值为1.09%(n=6).[结论]高效液相色谱法灵敏、简单、快速、准确,适用于六味地黄丸(浓缩丸)熊果酸含量的测定.     

砷钼杂多酸分光光度法测定三七中总砷含量

[目的]获得快速检测三七中总砷的方法.[方法]采用砷钼杂多酸分光光度法测定人工种植三七中的总砷含量,并优化其显色条件.[结果]最佳显色条件:显色剂用量为5.0 ml,显色温度为40℃,显色时间为30 min;AsⅢ标准溶液在浓度为0～0.04 μg/ml时,与吸光度呈良好的线性关系(r=0.996 6);样品中未检测到As.[结论]该方法精密度高,稳定性好,可作为三七中砷含量的检测方法.     

黄芪药渣中黄芪甲苷含量的测定

[目的]测定黄芪药渣中黄芪甲苷的含量.[方法]用高效液相色谱法,HPLC条件:Agilent Zorbax XDB-C<,18>(4.6 mm×150.0 mm,5μm)色谱柱,流动相为乙腈-水(35:65,v/v),流速1.0 ml/min,检测波长203 nm,柱温30℃,进样量10μl.[结果]黄芪甲苷在0.23-2.3μg/ml范围内与峰面积线性关系良好(r=0.999 9),平均回收率为99.00%,RSD值为2.00%(n=9),黄芪药渣中黄芪甲苷含量为0.74 mg/g.[结论]该方法快速、简便,黄芪药渣仍有较大的再利用价值.     

高效液相色谱-紫外检测法测定水产品中组胺的含量

[目的]建立一种测定水产品中组胺含量的高效液相色谱-紫外检测法.[方法]用高氯酸提取水产品中的组胺,丹磺酰氯衍生后,用配有紫外检测器的高效液相色谱仪进行测定,外标法定量.[结果]组胺在0.5-20.0μg/ml范围内线性关系良好,相关系数r>0.999.空白样品中组胺添加浓度在50～1000mg/kg时,平均回收率在81.O%～94.8%,批次内和批次间相对标准偏差均<10%.组胺的检出限为25 mg/kg,定量限为50 mg/kg.[结论]该方法操作简便,能够满足水产品中组胺含量的测定要求.     

环介导等温扩增技术快速检测志贺氏菌的研究

[目的]利用环介导等温扩增技术快速检测志贺氏菌.[方法]以志贺氏菌侵袭性质粒抗原H基因(ipaH)作为靶序列,设计引物,优化Mg<'2+>浓度、Bst酶浓度等反应条件,建立LAMP反应体系.[结果]确定了环介导等温扩增技术检测志贺氏菌的适宜反应条件;该法检测志贺氏菌的灵敏度为62 cfu/ml.[结论]该研究初步建立了一种利用LAMP快速检测志贺氏菌的方法,为食品中志贺氏菌快速检测构建了一个技术平台.     

HPLC测定不同采收期川楝子中阿魏酸的含量

[目的]测定不同采收期川楝子中阿魏酸的含量,并考察阿魏酸在采收期内的含量变化.[方法]采用高效液相色谱法,色谱柱为UltrasphereTM-ODS柱(4.6 mm ×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液(15:85=V:V),流速为1.0 ml/min,检测波长为322nm,柱温为25℃.[结果]不同采收期川楝子药材中阿魏酸含量无显著变化,以1月份采集的样品中阿魏酸含量最高.[结论]该方法简便、快速,可为川楝子确定最佳采收期提供科学依据.     

转炉炉壳热应力分析

根据实际转炉建立实体模型。以有限元为手段,考虑了炉衬和炉壳材料的物性参数随温度变化的特点和炉衬与炉壳之间膨胀间隙,计算了转炉炉壳在温度载荷和炉衬膨胀压力同时作用下的热应力。所得结果可为转炉炉壳设计提供依据。     

焦炭热性能指标意义浅析

通过对焦炭在高炉内的历程及其变化规律的剖析,阐述焦炭热性能指标的意义,形象地把焦炭在高炉内的损耗分为三部分(M1,M2,M3)。指出M2对焦比及焦炭价格有一定的指导作用。     

Results of treatment of cancer of the cardia

Cancer of the cardia is traditionally discussed with cardiac cancer of the lower portion of the esophagus and upper gastric cancer invading the esophagus, and the specific characteristics of cancer of the cardia have never been clearly defined. We reviewed the outcome of 172 patients with adenocarcinoma of the cardia who had undergone radical surgery between 1949 and 1994 in the Division of Gastrointestinal Surgery of the Cancer Institute Hospital. The centers of the tumors were located within 2 cm above and below the boundary between the esophagus and the stomach, and their longitudinal diameter was less than 8 cm. We divided the patients into an early period (1949-1979; n = 79) and a late period (1980-1994; n = 93), and focused on the historical transition. There were no differences in patient gender or histology between the two periods. However, the late period was associated with fewer cases of esophageal invasion and shorter longitudinal diameter, as the age of the population advanced. The number of advanced cancers such as the localized and infiltrative type had decreased, and early cancer and early clinical stage had become more common, but despite the fact that the number of early cancers had increased, extended dissection, such as thoracic and paraaortic lymph node dissection, was performed more frequently. By clinical stage, the long-term outcome markedly improved in Stage I patient in the late period, and tended to improve in Stage II and III patients. This appears to have been attributable to the prevention of micrometastasis by extended dissection, although the number of early cancers is another major potential cause. There were no differences in the outcome of Stage IV patients between the two periods, and further advances in multimodality therapy must be awaited. The range of resection is basically proximal gastrectomy, and if there are adequate indications, the prognosis is favorable. In view of the status of lymphatic flow and lymph node metastasis, and long-term results, lateroaortic lymph node dissection is important. Since the number of early cancer patients has been increasing, if intraabdominal recurrence is prevented, intrathoracic lymph node dissection will contribute greatly to the outcome of such patients.     

The effect of amount of possession of the trait of hostility on accuracy of perception of hostility in others.

Some theories of personality hold that an individual's perception of others is influenced by his own personality characteristics; more specifically, that a trait of low social value will be projected more into others, and that insight into the possession of these characteristics reduces this distortion. These 2 hypotheses were tested by having a group of college students rate each other (including themselves) on degree of friendliness or hostility. Agreement with group rating about self yielded the insight score. In general, the hypotheses were supported; the "friendly" Ss were most accurate in their perception of others, and insight was positively associated with accurate perception. From Psyc Abstracts 36:04:4HL16M. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Comparison of rates of infection of two methods of emergency ventricular drainage

The rates of infection of two methods of external ventricular drainage in use at Atkinson Morley's Hospital--namely, (a) percutaneous drainage with Rickham reservoirs and (b) tunnelled ventriculostomies--were compared in this retrospective review. Percutaneous drainage of CSF with Rickham reservoirs was associated with a 27% rate of infection as identified by positive microbiological cultures; tunnelled ventriculostomy catheters had a 10% infection rate. The difference in the infection rate between the two methods was statistically significant (P < 0.015). Other variables examined, including the age and sex of the patients and the reasons for ventricular drainage, were not associated with an increased rate of infection. Most infections from either method were caused by a coagulase negative staphylococcus. The average duration of ventricular drainage before identification of positive cultures was 5.7 days for Rickham reservoirs and 6.0 days for ventriculostomies.     

浅析《野草》的版画特征

鲁迅先生一生热爱美术作品,对版画中木刻兴趣尤甚.<野草>作为其"写心之作",亦呈现出一些与木刻版画一致的审美特征:一是具有木刻版画的色彩特征,多对比、多冷色,有阴郁的美感;二是具有木刻版画的力度特征,环境描写、人物刻画处处刀锋毕现,遒劲有力,有一种"力之美".     

四辊破碎机车刀架的改造

针对四辊破碎机车刀架车削中存在的几个问题,详细介绍了电动车刀架的结构和工作原理,建立主要设计参数的数学表达式,并进行适当的分析。改造后,这种电动车刀架运行平稳、可靠、方便、省力,取得了预期的效果。     

Effect of antibiotics of chemotaxis of human leukocytes

The effect of 20 different antibiotics on chemotaxis by human neutrophils was studied. Human leukocytes incubated with chloramphenicol, rifampin, sodium fusidate, and tetracyclines in vitro showed markedly depressed migration. The mechanisms by which these antibiotics affect leukotaxis are discussed.