血液分析仪检验急慢性白血病的临床分析

目的:探讨血液分析仪检验急慢性白血病的临床效果.方法:40例白血病患者(慢性白血病6例,急性白血病34例)与健康体检人40例分别采用贝克曼 DxC 600 Synchron型血细胞分析仪进行血液学检验.结果:通过检测分析,急慢性白血病的外周血血红蛋白都低于对照组,而CRP、白细胞值都明显高于对照组,差异有显著性(P>0.05).不过上述指标值在急慢性白血病间差异无显著性(P<0.05).结论:血液分析仪在白血病患者的血液检验效果好,可得到比较准确的诊断结果,值得推广应用.     

鲤鱼白细胞介素-1β全长cDNA的克隆·鉴定及其差异表达分析

[目的]进行鲤鱼白细胞介素-1β(IL-1β)全长 cDNA 的克隆、鉴定及其差异表达分析.[方法]利用 DD-RTPCR 方法获得差异表达 cDNA 片段,时有丝分裂原刺激的鲤鱼外周血白细胞 cDNA 文库进行筛选,克隆了鲤鱼 IL-1β的全长 cDNA,并进行了序列分析和差异表达分析.[结果]获得的阳性克隆含有 1 个大小为 831 bp 编码 276 个氨基酸的完整开放阅读框.聚类分析表明,鲤鱼 IL-1β氨基酸序列与日本鲤鱼紧密聚为一支,氨基酸序列的同源性达 95%,之后聚类顺序依次为鲫鱼、斑马鱼、猪、牛、马、人和小鼠.差异表达分析表明,经有丝分裂原刺激后前期(4 h)白细胞中 IL-1β的表达量显著增大,但随着时间推移(12,24 h)并非一直较同期大,表达量总体趋势成峰形.[结论]为进一步研究 IL-1β在体内的表达方式、功能特点和调控机理以及在炎症反应、应急反应和免疫应答的作用机制奠定了基础.     

人血白蛋白治疗急性脑出血的临床疗效及安全性评价

目的:探讨人血白蛋白治疗急性脑出血的临床疗效.方法:将我院2009年6月至2010年6月收治的80例急性脑出血患者随机分为观察组和对照组,每组40例,对照组在常规治疗的基础上采用川穹嗪注射液静脉滴注,观察组在常规治疗的基础上采用10%人血白蛋白静脉推注,两组患者均以14d为一个疗程,比较两组患者的临床疗效、日常生活活动能力(ADL)及神经缺损评分.结果:观察组基本痊愈16例,显著进步12例,进步6例,总有效率为92.5%(37/40)显著高于对照组80.0%(32/40),P<0.05.且观察组的ADL、神经功能缺损评分均显著优于对照组,P<0.05.结论:人血白蛋白治疗急性脑出血疗效确切,值得临床推广.     

奥扎格雷纳联合低分子肝素钠治疗急性脑梗塞的临床分析

目的:观察奥扎格雷纳联合低分子肝素钠治疗急性脑梗塞的临床效果.方法:对某医院自2008年3月~2010年7月收治的126例急性脑梗塞患者进行回顾性分析,所有患者均在常规治疗的基础上给予奥扎格雷纳联合低分子肝素钠治疗,观察本组患者的治疗效果.结果:①本组126例患者ESS评分随着时间增长逐渐得到改善,从治疗2h开始即较治疗前有明显差异,治疗8、14、48h时均较治疗前有显著差异(P<0.01);凝血酶(TT)及凝血酶原时间(PT)治疗前与治疗48h无明显差异,治疗过程中出现波动,治疗8h达到高峰,以后逐渐下降.②本组患者治疗过程中未出现明显不良反应.结论:奥扎格雷纳联合低分子肝素钠治疗急性脑梗塞效果理想,值得临床推广应用.     

Relationship between prostaglandin E2 concentrations in periapical exudates from root canals and clinical findings of periapical periodontitis

In this study the relationship of prostaglandin E2 (PGE2) concentrations in periapical exudates with clinical findings of teeth with periapical periodontitis is discussed. Periapical exudate samples were obtained from root canals of 77 endodontically involved teeth during routine root canal treatment by the quantitative sampling method using paper points. PGE2 concentrations in periapical exudates (PE-PGE2) were determined by radioimmunoassay. Significantly higher levels of PGE2 were found in periapical exudates from teeth with radiolucent areas than from teeth without radiolucent areas (236.8 +/- 521.3 pg/microliter vs. 14.9 +/- 23.1 pg/microliter, respectively). The elevated PE-PGE2 levels were associated with the presence of clinical symptoms that reflected an acute inflammation in the periapical lesion. In contrast, a significantly negative association of decreased PE-PGE2 levels with increasing size of radiolucent areas was demonstrated. These results suggested that PGE2 was produced locally in periapical lesions and that the PGE2 concentration in periapical exudate could reflect the state of the disease activity in periapical periodontitis.     

封闭式负压引流术治疗皮肤脱套伤的护理

封闭式负压引流就是利用VSD敷料(国内俗称人工皮)填充创面、创缘,敷料连同其内的引流管,粘贴上半透膜,使之处于封闭状而进行的引流.持续负压引流能彻底清除腔隙或创面各种引流液及坏死组织,确保创面洁净,加速水肿消退,促进创面肉芽组织生长.封闭式引流是一种理想的引流方法,是引流技术的革新.我院2007年4至2010年4月对56例皮肤脱套伤广泛使用VSD覆盖,并予负压持续吸引治疗,效果明显.     

功能锻炼结合改良的封闭式负压引流Ⅳ期压疮的护理效果

目的:探讨Ⅳ期压疮治疗中功能锻炼的地位及应对方法.方法:对我科Ⅳ期压疮的病人进行功能锻炼的时机和方法.结果:Ⅳ期压疮治愈率88%,肢体康复47%,能下地活动60%,能坐轮椅40%.结论:通过本组病例的护理,认为功能锻炼对Ⅳ期压疮的治疗起到良好的辅助作用(应用封闭式负压引流的观察护理).     

58例复方甲硝唑糊剂治疗根尖周病的临床分析

目的:总结研究复方甲硝唑糊剂治疗牙根尖周病的临床疗效.方法:收集近年来经我院治疗的58例患者进行牙片检查,根据患者有无瘘管情况分别治疗.有瘘管的患者在无叩痛、根管无渗出物后,用氧化锌丁香油糊剂中加入碘仿、甲硝唑(灭滴灵)的配方进行根管填充,无瘘管的患者即刻填充.结果:本组治愈:55例(94.8%);好转:2例(3.4%);无效:1例(1.7%).结论:运用在氧化锌丁香油糊剂中加入碘仿、甲硝唑(灭滴灵)进行根管填充的方法治疗根尖周病疗效显著,值得推广.     

胸腔闭式引流临床治疗的观察与护理

目的:观察胸腔闭式引流对治疗胸部疾患的效果.方法:应用整体护理的模式对我院102例需要进行胸腔闭式引流的患者进行护理,掌握好每个患者的具体病情和心理,通过护理措施有的放矢的对患者进行护理.结果:97例需做胸腔闭式引流的患者护理效果良好(治愈率达95.1%),顺利治愈出院.结论:应用胸腔闭式引流治疗胸部疾患安全简便,临床疗效满意.     

Cytotoxic effect of endodontic bacteria on periapical fibroblasts

This study was conducted to investigate the effects of sonicated bacterial extracts (SBEs) from anaerobic Gram-negative bacteria on periapical fibroblast obtained from the apical portion of human periodontal ligaments. Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum were chosen from among the endodontic bacteria isolated from root canals having a periapical lesion and compared in terms of their cytotoxicity. The purpose of this study was to examine which bacteria are involved in the development of periapical inflammation. The anaerobes were cultured under strict anaerobic conditions, and the bacterial cells were then harvested by centrifugation after incubation. The concentrated cell suspensions were sonicated and subsequently centrifuged. An SBE was made of each of the filtered supernatants. Each SBE was added to cultures of periapical fibroblasts. The cell growth and proliferation were measured by the MTT method after 3, 5, and 7 days. The SBEs from P. endodontalis, P. gingivalis, and F. nucleatum inhibited the growth of the fibroblasts, whereas the SBE from P. intermedia did not inhibit it. The SBEs from P. gingivalis and F. nucleatum inhibited the fibroblast growth more strongly than did the P. endodontalis, P. gingivalis, and F. nucleatum may participate in the development of periapical lesions.     

Comparison of the efficacy, safety and tolerability of azithromycin and co-amoxiclav in the treatment of acute periapical abscesses

The activity, safety and tolerability of the azalide azithromycin were compared with those of co-amoxiclav in the treatment of acute periapical abscesses in adults in an open, randomized, multicentre comparative study. Patients of either sex, recruited from 106 dental practices in Belgium, were aged between 18 and 75 years and had acute periapical abscesses not requiring drainage, confirmed by radiology. Azithromycin was administered as a 500-mg tablet orally once daily for 3 days (n = 150) and co-amoxiclav as a 625-mg capsule three times daily, for 5-10 days (n = 153). Both before and after treatment, masticatory pain, percussion pain, headache, and oedema and redness of soft tissue were graded on a four-point scale. Overall clinical success (cure plus improvement) was seen in 131/144 (91%) evaluable patients receiving azithromycin and in 142/148 (96%) receiving co-amoxiclav (difference not significant). There was no significant difference between the two groups in the incidence or severity of adverse events or in the number of discontinuations because of adverse events.     

The effect of a pressure garment on post-surgical drainage and seroma formation in breast cancer patients

BACKGROUND: The treatment of choice for cancer of the breast are mastectomy and axillary clearance or wide excision with axillary clearance. The most common complication following such a procedure is seroma formation. Various methods have been used to prevent it. However, the use of a pressure garment in this context has not been fully evaluated in the current literature. RESULTS: In a randomised trial comparing patients with a pressure garment with those without one, we have found no improvement in post-operative drainage with the use of a pressure garment. There were more complications in the group without the pressure garment (19%) compared with those with the garment (15.7%). The only seroma occurred in those patients not using the garment. The use of the pressure garment appeared to increase the duration of use of the drain (6.8 vs 6.1 days), these differences in the two groups was not significant. One of the patients in the pressure garment group was unable to tolerate the warmth and discontinued wearing the garment in the third post-operative day. This gave a rejection rate of 5%. CONCLUSION: The use of a pressure garment does not reduce the post-operative drainage, however, the complication rate appears to be higher when the pressure garment is not being used.     

Ultratonotherapy in the combined treatment of chronic periodontitis

The trial of apex-locator application for improvement of endodontic management of root canals of a tooth as well as of ultratonotherapy in 120 patients aged 20-40 years with chronic granulomatous and granulating periodontitis has proved that ultratonotherapy relieves inflammation and stimulates regeneration of bone tissue.