Effects of knife-cut lesions of the medial forebrain bundle in self-stimulating rats.

Unilateral frontal-plane knife-cut lesions were made in the anterior medial forebrain bundle ipsilateral to a lateral hypothalamic self-stimulation electrode. Behavioral effects of the knife cut on self-stimulation reward and operant performance capacity were measured via the reward summation function method. Knife cuts placed at the level of the anterior commissure were ineffective in altering reward or motor/performance capacity, whereas knife cuts just posterior in the caudal lateral preoptic area degraded reward and sometimes impaired motor/performance capacity. In a second experiment, knife cuts placed posterior to the ventral tegmental area were ineffective unless they intruded on the ventral tegmental area itself. Several small knife cuts placed just anterior to the ventral tegmental were effective in reducing self-stimulation reward. The results are discussed in terms of the anatomical substrate of lateral hypothalamic self-stimulation reward and as a first step in a larger mapping study. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Neurturin protects dopaminergic neurons following medial forebrain bundle axotomy

To determine whether neurturin (NTN), a recently identified homologue of glial cell line-derived neurotrophic factor (GDNF), is able to preserve tyrosine hydroxylase immunoreactivity (TH-IR) in a rat model of Parkinson's disease, polymer encapsulated cells genetically engineered to release NTN were implanted near the substantia nigra 1 week before a unilateral medial forebrain bundle axotomy. Animals were allowed to survive for 1 week post-axotomy. Upon sacrifice, animals that received a NTN capsule had a significantly higher percentage of TH-IR (lesioned side vs non-lesioned side) than animals that had received a capsule containing non-transfected parent cells. However, in contrast to GDNF, no reduction of turning was observed upon amphetamine rotation with NTN. Nevertheless, these results suggest that NTN might have a therapeutic value for the treatment of Parkinson's disease.     

Effects of rostral medial forebrain bundle and olfactory tubercle lesions upon sexual behavior of male rats.

Examined the effect on sexual behavior of 57 male Sprague-Dawley rats of reduced olfactory system input to the preoptic area and medial forebrain bundle (MFB). Bilateral electrolytic lesions in the MFB just caudal to the preoptic area virtually abolished mating behavior. Electrolytic lesions or sham lesions placed bilaterally in the olfactory tubercle produced decrements in the mating behavior. There was no evidence of gonadotropic dysfunction in any of the groups, nor were there significant differences in their mean body weights at sacrifice. (22 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Running-wheel avoidance behavior following septal area lesions in rats.

Studied acquisition of a running-wheel avoidance response in 52 male Wistar rats with (a) septal lesions, (b) septal and postcommissural fornix lesions, or (c) septal, postcommissural fornix, and anterior thalamic damage. Ss with lesions confined to the septum were deficient in acquiring the avoidance response compared with both normal Ss and Ss with the more posterior lesions. Ss sustaining combined septal-fornical and septal-fornical-thalamic lesions did not differ from normal Ss or from each other in their acquisition scores. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effect of lesions in the medial forebrain bundle on three measures of pain sensitivity and noise-elicited startle.

Subjected 137 male Holtzman albino rats to bilateral lesions of the medial forebrain bundle (MFB) or to surgical control procedures. Lesions produced equivalent decreases in paw-lick latency to heat and in jump and high-magnitude-response thresholds to footshock. Flinch and detection thresholds to footshock were also significantly decreased, though the lesion did not alter the magnitude or habituation of noise-elicited startle. Injection of 75 mg/kg serotonin into lesioned Ss returned the high-magnitude-response threshold to normal values but did not affect detection threshold. It is concluded that the increased sensitivity to painful stimuli resulted from the decrease in telencephalic content of serotonin following MFB lesions. Previous exposure of lesioned Ss to footshock resulted in a potentiation of the startle response to noise, further suggesting that the lesion had increased the aversiveness of footshock. (27 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Slower acquisition of positively reinforced behavior following medial, but not dorsolateral, septal lesions in rats.

Examined the effects of medial septal lesions (MSLs) and dorsolateral septal lesions (DSLs) on acquisition of leverpressing for water established with a modified autoshaping task. 22 male Sprague-Dawley rats were randomly assigned to receive either MSLs, DSLs, or control operations. Results show that, following MSLs, leverpressing responses had long latencies and were fewer than following either DSLs or control operations. However, during later sessions, MSL Ss started to press. It is suggested that the long response latencies and low response rates shown initially by MSL Ss may have been due to delayed classical conditioning. Following MSLs, the acetylcholinesterase activities of the different subdivisions of the hippocampus correlated positively with the number of leverpress responses emitted during Session 1. DSL Ss acquired the task just as fast as the control group and showed higher response rates than the other 2 groups during the final sessions. These findings are ascribed to generally increased locomotor activity. (24 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Age-related deficits in acquisition of a passive avoidance response.

In Exp. I, 80 male albino rats 10, 15, 20, 30, or 100 days old received a brief inescapable shock contingent upon making a step-off response. Step-off latencies increased for all age groups, but rate of learning was significantly faster in older Ss. Learning appeared to be based primarily upon punishment effects rather than general emotionality, since yoked Ss shocked after being placed directly on the grids did not acquire the avoidance response. Exp. II with 120 Ss employed 3 training conditions with independent groups 12, 15, 18, or 21 days old. The step-off response resulted in shock that was either: (a) escapable; (b) inescapable, 1-sec duration; or (c) inescapable, yoked duration. Younger Ss were again significantly inferior to more mature Ss. Escapable shock improved acquisition at 2 age levels, but the effect appeared to be more related to shock duration than to the response contingency. It is suggested that the requirement of withholding a punished response may represent a category of learning that is especially sensitive to maturational changes. (French summary) (16 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Medial forebrain bundle lesions: Specific loss of feeding to decreased glucose utilization in rats.

Found that bilateral destruction of the medial forebrain bundle in female Sherman rats eliminated feeding to decreased intracellular glucose utilization (glucoprivation). The deficit was specific. Feeding was enhanced by dietary dilution and reduced by dietary concentration. More was eaten in the cold and less in the heat. The Ss were not differentially sensitive to quinine adulteration. They returned to normal body weight following regimens of gavage or of restricted feeding. Moreover, they did not differ from normal Ss in drinking to various thirst stimuli. It is suggested that the glucoprivic mechanism makes but a minor contribution to the initiation of spontaneous feeding. (39 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effects of varying active/passive shock levels in shuttle box avoidance in rats.

Ran 60 male Sprague-Dawley albino rats in a shuttle box with 3 levels of shock intensity-high (H), medium (M), and low (L)-which varied independently on each side. This yielded 6 groups defined by the shock level in the 2 chambers: H-H, M-M, L-L, H-L, H-M, M-L. Results indicate that when active shock level was held constant, avoidance behavior was a function of passive shock level, with lower passive shock level producing higher levels of avoidance. Avoidance was not a function of active shock level when passive shock level was held constant. 10 controls were run using a unidirectional paradigm, and avoidance was not found to be a function of the active shock level. Results are interpreted as emphasizing the role of passive avoidance in bidirectional shuttling. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Anatomical dissociation of the substrates of medial forebrain bundle self-stimulation and exploration.

The purpose of this research was to determine whether brain stimulation reward and exploration are induced by activation of the same set of neurons along the medial forebrain bundle. The behavioral version of the collision test was utilized with electrodes in the lateral hypothalamus (LH) and the ventral tegmental area (VTA). A collision effect obtained between LH and VTA in one behavior at the exclusion of the other was treated as evidence of the involvement of two different sets of fibers. In 4 rats, a collision effect was observed only in self-stimulation, whereas in 1 rat, a collision was obtained in exploration at the exclusion of self-stimulation. Three animals showed no collision in either behavior. These data suggest that coexistence of self-stimulation and exploration following medial forebrain bundle stimulation can be explained by current spread on two different sets of fibers. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Electrolytic lesions of the cortical and adjacent nuclei in the amygdala differentially influence thresholds for rewarding medial forebrain bundle stimulation.

After electrolytic lesions to the cortical and adjacent amygdaloid subnuclei, thresholds for rewarding medial forebrain bundle (MFB) stimulation were tracked in 19 rats with bilateral implants and 8 with single implants. Results were categorized into 3 groups depending on the magnitude of the lesion effect on ipsilateral frequency thresholds: substantial (> 60%), small (> 26%), or none (     

Increased expression of NGFI-A mRNA in the rat striatum following burst stimulation of the medial forebrain bundle

Using in situ hybridization, we examined the mRNA expression for several immediate early genes in dopamine-innervated brain areas following electrical burst vs. regular stimulation of the medial forebrain bundle in anaesthetized rats. Two hours after 5 Hz burst stimulation, the expression of the nerve growth factor-inducible clone A (NGFI-A) mRNA was increased in the medial part of the striatum. This increase was prevented by pretreatment with the dopamine-D1 receptor antagonist, SCH23390 (0.1 mg/kg i.p.). After 8 Hz burst stimulation, NGFI-A mRNA expression was increased in the medial, central and lateral parts of the striatum. Induction occurred predominantly in cells expressing mRNAs for the dopamine-D1 receptor, substance P and dopamine and cAMP-regulated phosphoprotein (DARP-32). Regular stimulation had no effect on NGFI-A mRNA expression. The induction of NGFI-A was related to the levels of dopamine released by burst or regular stimulation as demonstrated with in vivo amperometry. Two hours after stimulation, the expression of none of the other genes studied was altered. One hour after 8 Hz burst stimulation, the expression of NGFI-A, NGFI-B and jun-B mRNAs was increased in the striatum and that of NGFI-A, NGFI-B, c-fos, fos-B and jun-B mRNAs was variably increased in the nucleus accumbens and lateral septum. These results provide additional support for the physiological importance of burst firing activity in midbrain dopamine neurons for the activation of their target cells. They demonstrate a spatial and temporal specificity as regards the brain region, the gene activated, the receptor involved and the phenotype of the cells affected.     

Species-specific behavior and acquisition of avoidance behavior in rats.

Studied thigmotaxis (i.e., the tendency to run in contact with objects) in 3 experiments with a total of 54 female Long-Evans rats. Exp. I indicated that Ss became more thigmotactic and immobile following shock compared with no-shock conditions. Exp. II demonstrated that when 2 groups were required to make comparable, but different, avoidance responses, the group whose avoidance response was more closely related to the S's species-specific defensive behavior was acquired at a faster rate. The 3rd experiment indicated that the differences in the acquisition of the avoidance responses in Exp. II were not due to differences in operant rates for the 2 responses per se, since acquisition of these same 2 responses was similar under appetitive motivation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effects of amygdaloid lesions on the performance of rats in four passive avoidance tasks

The blocking effect of apomorphine on the rise in striatal dopamine (DA) content, induced by 1-hydroxy-3-amino-pyrrolidone-2 (HA-966) was taken as a measure for the intrastriatal feedback inhibition of DA synthesis. The effects of cholinergic drugs on this feedback system were assessed in order to verify the hypothesis that this mechanism is mediated via an intrastriatal cholinergic link. We presumed that DA receptors were located on a cholinergic neuron, while the cholinergic terminals in turn made direct or indirect axon-axonal contact with the dopaminergic nigro-striatal pathway (N.S.P.). Although cholinergic agents could modify the effect of HA-966 on striatal DA content, it proved to be impossible to counteract the blocking effect of apomorphine with cholinergic drugs as was to be expected. Therefore we concluded that the effect of apomorphine was not brought about in the way which had been postulated.     

Prolonged rewarding stimulation of the rat medial forebrain bundle: Neurochemical and behavioral consequences.

Extracellular dopamine levels were measured in the rat nucleus accumbens by means of in vivo microdialysis. Delivery of rewarding medial forebrain bundle stimulation at a low rate (5 trains/min) produced a sustained elevation of dopamine levels, regardless of whether train onset was predictable. When the rate of train delivery was increased to 40 trains/min, dopamine levels rose rapidly during the first 40 min but then declined toward the baseline range. The rewarding impact of the stimulation was reduced following prior delivery of stimulation at the high, but not the low, rate. These results support the idea that dopamine tone plays an enabling role in brain stimulation reward and is elevated similarly by predictable and unpredictable stimulation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)