共查询到19条相似文献,搜索用时 140 毫秒
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目的建立一种简单、准确的检测牛乳中抗生素残留的方法-乳酸菌发酵法。方法以接种量和温度为变量,优化发酵条件;以青霉素为目标抗生素,建立检测方法;以发酵4 h未凝乳的样品中抗生素最低浓度为检测限,测定本方法对20种抗生素的检测限;对比本方法和GB/T 4789.27方法检测111个商品乳样品中抗生素残留检出率,验证本方法的准确性。结果建立了乳酸菌发酵法检测乳中抗生素残留的具体方法;确定了本方法对8类20种抗生素的检测限,其中4类12种抗生素的检测限低于其在牛乳中的最大残留限量(MRL);本方法和GB/T 4789.27方法检测111个商品乳样品抗生素残留检出率分别为9.0%和11.7%。结论本方法操作简单,结果准确,适合企业及基层检测单位作为初筛方法检测牛乳中的抗生素残留。 相似文献
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广州市牛奶中抗生素残留的现状分析 总被引:15,自引:0,他引:15
为了控制牛乳中抗生素的残留,对广州市牛奶中抗生素残留进行监测。结果表明不同给药途径及剂量可造成不同的残留时间;生牛奶及奶粉中抗生素残留阳性率显著比消毒牛奶高;出口生牛奶抗生素残留阳性率显著比内销生牛奶高;进口消毒牛奶及奶粉中未检出抗生素。 相似文献
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目的建立高效液相色谱-三重四级杆质谱联用法检测我国南方地区荷斯坦和水牛原料乳中喹诺酮类抗生素类残留的分析方法。方法采集南方地区15个荷斯坦牧场和15个水牛牧场的120份原料乳,采用高效液相色谱-三重四级杆质谱联用法检测其中的喹诺酮类抗生素残留情况。结果检出限和回收率分别为0.05~0.25μg/L和88.2%~109.2%。水牛乳中喹诺酮类抗生素残留检出率为8.06%,荷斯坦牛乳中抗生素残留率为6.89%。结论该方法检测牛乳中喹诺酮类结果准确可靠,南方地区的荷斯坦牛乳和水牛乳喹诺酮类残留量与国内外相比程度较低,原料乳总体上处于安全范围。 相似文献
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乳和乳制品中抗生素残留的危害及检测方法 总被引:3,自引:1,他引:3
综述了乳与乳制品中抗生素残留现状和危害,比较了抗生素检测Delvotest法与其他几种方法。认为Delvotest法优于其他几种方法,建议有关部门尽快引进该技术,以加强我国乳和乳制品中的抗生素监测,解决目前乳业中面临的难题。 相似文献
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乳腺炎是奶牛的常见病,通常需要抗生素治疗,因此不可避免地造成牛奶中的抗生素残留。由于免疫传感器是将高灵敏的传感器技术与抗原、抗体特异性反应相结合的一种检测方法,易于实现抗生素残留检测仪器的便携化、微型化和自动化,因而成为近年来研究的热点。本文介绍免疫生物传感器的基本原理、组成与分类,重点介绍国内外应用免疫传感器检测牛奶中抗生素残留的研究进展,并分析未来免疫生物传感器的发展趋势。 相似文献
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《Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment》2013,30(6):1001-1008
The use of antibiotics in livestock can pose a public health threat, especially if antibiotic residues remain in the food product. Understanding how often and why farmers sell products with antibiotic residues is critical to improving the quality of these products. To understand how often milk with antibiotic residues is sold on small farms in a major dairy-producing region of Peru and identify factors associated with selling milk with antibiotic residues, we tested milk samples for antibiotic residues from every provider on three routes of commercial milk companies and from bulk tanks of farmers currently treating cows with antibiotics. We also asked farmers if they sold milk from treated cows and examined factors associated with the tendency to do so. The prevalence of milk contamination with antibiotic residues on commercial routes was low (0–4.2%); however, 33/36 farmers treating their animals with antibiotics sold milk that tested positive for antibiotic residues. The self-reported sale of milk from treated cows had a sensitivity, specificity, and positive and negative predictive values of 75.8%, 100%, 100% and 27.2%, respectively (with testing of milk for residues as the gold standard). Finally, 69/156 randomly selected farmers reported selling milk from treated cows, and farmers’ knowledge of antibiotics and the milk purchaser were significantly associated with a farmer’s tendency to report doing so. Educating farmers on the risks associated with antibiotics and enforcement of penalties for selling contaminated milk by milk companies are needed to improve milk quality. 相似文献
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为了预防与治疗乳牛疾病,抗生素类兽药被广泛应用于乳牛的饲养过程中。而滥用抗生素导致牛奶中抗生素残留超出最大允许残留量,不仅会对牛奶品质造成影响,而且可能会危害到牛奶饮用者的身体健康。因此,如何快速、准确地检测乳与乳制品中抗生素类兽药残留是乳品检测行业和企业亟需解决的问题。本文主要介绍了牛乳中兽药残留的来源、危害及其现状,阐述了酶联免疫分析技术、胶体金免疫层析技术的检测原理及其在应用过程中所具有的不同特点,最后对2种方法的优势与不足进行简要总结,并对免疫分析法在乳与乳制品抗生素类兽药残留检测中的应用前景做出展望。 相似文献
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随着抗生素在动物饲养过程中的普遍应用, 抗生素的不合理使用现象日益普遍, 造成动物源性食品(牛奶、肉类、水产品、鸡蛋、蜂蜜等)中抗生素的残留, 从而对人体健康造成潜在的危害, 因此动物源性食品中抗生素残留检测是控制食品质量安全的重要环节。本文对动物源性食品中抗生素残留的现状, 抗生素残留的种类及检测方法作了阐述, 并对抗生素检测方法的应用前景进行了展望, 以期为有关部门在检测动物源性食品中抗生素的残留的工作提供技术参考。 相似文献
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采用荧光免疫层析法结合现场检测仪建立牛奶中头孢氨苄残留的快速定量检测方法。方法 利用反向微乳技术合成稀土铕荧光纳米颗粒,与头孢氨苄单克隆抗体结合制备荧光检测探针,以头孢氨苄卵清蛋白全抗原和羊抗小鼠抗体分别作为检测线和质控线制备免疫层析试纸条,结合现场检测仪建立牛奶中头孢氨苄残留的快速定量检测方法。结果 试验结果表明,该方法对头孢氨苄的检测限为0.16 ng/ml,半数抑制浓度(IC50)为0.6 ng/ml,线性范围为0.16~5 ng/ml,标准添加回收率为100%~115%之间,与头孢菌素类及青霉素等其他12种抗生素的交叉反应率均<0.01%,与ELISA方法比较,测定结果相关性良好。结论 本试验所建立的荧光免疫层析法快速检测牛奶中头孢氨苄残留,具有简便、快速、灵敏、直观的优点,可用于抗生素残留的筛查,极具推广和应用价值。 相似文献
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Cows from five dairy herds were used to determine persistence of antibiotic residues in colostrum and milk following dry cow therapy. Cows were treated in all quarters at drying off with antibiotics approved for use for nonlactating cows. Antibiotics procaine penicillin G plus dihydrostreptomycin, novobiocin, cloxacillin, or cephapirin were compared with no treatment. Composite colostrum samples were collected from each cow at first milking after parturition. Samples were screened for residues by Delvotest P. Colostrum samples positive by Delvotest also were tested by Bacillus stearothermophilus disc assay. Four of 186 colostrum samples from cows treated with antibiotics at drying off were positive for residues by Delvotest. Only one was confirmed positive by disc assay following heat treatment. All colostrum samples from 48 cows not treated were negative. Samples of first marketable milk also were collected. Over 96% of milk samples from cows treated at drying off and 100% of milk samples from cows not treated were negative for residues by Delvotest. If manufacturer's recommendations are followed, antibiotic residues in colostrum and milk following dry cow therapy with products in our study should not be a significant problem. 相似文献
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The improved Dutch tube diffusion test was used to study the occurrence of inhibitory substances in raw bulk milk samples within the Nakuru District in Kenya. Initially the detection limits of the method were verified using milk standards spiked with selected antibiotics. Addition of penicillinase to inhibitor-positive samples was used for preliminary identification of penicillin G-type antibiotics and residue levels were estimated against a standard curve constructed by means of a B. stearothermophilus disc assay. The two-tube test was used to screen 1109 field samples of which 229 (21%) were suspect positive. The identification procedure confirmed 165 samples (14.9%) to contain penicillin G-type residues of which 118 contained levels exceeding the established EU MRL for penicillin G (4 μg/kg). This study indicates that antibiotic residues are prevalent in milk within the Nakuru district of Kenya. It suggests that the improved tube diffusion test in combination with a multiplate system could be useful for qualitative and quantitative identification of antimicrobial drug residues in milk. 相似文献