Effects of concentrate location on the behavior and production of dairy cows milked in a free-traffic automated milking system

The objective of this study was to determine whether the amount of concentrate allowance in an automated milking system (AMS) affects partial mixed ration (PMR) sorting behavior, milking activity, and production of lactating dairy cows fed isocaloric diets. Fifteen primiparous Holstein cows were used in a crossover design with 28-d periods, including 14 d of adaptation and 14 d of data collection. The cows were housed in a freestall pen with free-traffic access to the AMS. Treatments consisted of a higher-concentrate PMR (H-PMR) with a pelleted concentrate allowance of 3.0 kg/d on a dry matter (DM) basis in the AMS, or a lower-concentrate PMR (L-PMR) with a pelleted concentrate allowance of 6.0 kg/d in the AMS. As designed, cows on the L-PMR had greater AMS concentrate intake (6.3 vs. 3.1 kg/d of DM) compared with the H-PMR. The standard deviation for mean concentrate intake among days increased from 0.38 to 1.0 kg/d with greater targeted AMS concentrate intake. When fed the L-PMR diet, PMR intake was reduced compared with when cows were fed the H-PMR diet (17.1 vs. 19.1 kg/d of DM). The reduction in PMR intake was compensated for by greater AMS concentrate intake; thus, cows on the L-PMR had greater total dry matter intake (DMI; 23.6 vs. 22.3 kg/d). Cows sorted against long (>19 mm) and fine (<4 mm) PMR particle fractions, and in favor of medium (8 to 19 mm) and short (4 to 8 mm) PMR fractions when on the H-PMR treatment, but only sorted against the medium and in favor of the short PMR fractions on the L-PMR treatment. PMR eating rate and total time spent eating PMR did not differ significantly between the 2 treatments; however, meal size tended to be larger when cows were fed the H-PMR compared with the L-PMR (2.2 vs. 2.1 kg DM/meal). Cows tended to spend 30.8 min/d more time lying down when fed the L-PMR. On the L-PMR treatment, cows tended to have more voluntary AMS visits (5.9 vs. 4.6 visits/d), were fetched less (0.1 vs. 0.5 times daily), and had a greater milking frequency (3.5 vs. 3.0 milkings/d) compared with when they were on the H-PMR treatment. However, milk yield was not affected by treatment. These data suggest that allocating a greater proportion of total dietary concentrate to the AMS, in a free-traffic setup, may improve milking activity and decrease the need for fetching, as well as promoting greater amounts of and maintaining consistency in total dry matter consumption.     

Effect of the amount of concentrate offered in an automated milking system on dry matter intake,milk yield,milk composition,ruminal digestion,and behavior of primiparous Holstein cows fed isocaloric diets

The objective of this study was to determine if the quantity of concentrate provided in an automated milking system (AMS) affects dry matter intake (DMI), attendance to the AMS, milk and milk component yield, feeding behavior, cow activity, and ruminal fermentation of lactating dairy cows fed isocaloric diets. Eight ruminally cannulated primiparous Holstein cows were used in a replicated 4 × 4 Latin square design with 28-d periods. Cows were housed in a freestall facility with a guided-traffic (feed-first) flow barn design. Treatments included 0.5, 2.0, 3.5, or 5.0 kg/d of dry matter of pellet in the AMS with an equivalent reduction of the same pellet in the partial mixed ration (PMR). Days 21 to 24 of each treatment period were used for DMI, milking performance (visits, yield, and composition), behavior, and ruminal pH determination, and d 25 to 28 were used for ruminal short-chain fatty acid and ammonia concentrations as well as total-tract digestibility. As imposed, consumption of AMS pellet linearly increased, equating to 0.50, 2.00, 3.49, and 4.93 kg of dry matter/d for the 0.5, 2.0, 3.5, and 5.0 kg/d treatments, respectively. Correspondingly, the standard deviation in AMS pellet intake among days linearly increased from 0.06 to 0.85 kg of dry matter/d as the quantity of concentrate in the AMS increased from 0.5 to 5.0 kg. The PMR DMI decreased linearly with increasing AMS concentrate allocation, but total DMI (PMR + AMS) was not affected (25.3 kg/d). As the AMS concentrate allocation increased, the selection against particles retained on an 18-mm sieve linearly increased and selection against particles retained on the pan decreased. Milking frequency (3.22 milkings/d), milk yield (37.5 kg/d), milk fat yield (1.43 kg/d), and milk protein yield (1.22 kg/d) were not affected; however, milk urea nitrogen concentration decreased linearly with increasing AMS concentrate. Ruminal pH averaged 6.18 and was not affected by AMS concentrate. Total ruminal short-chain fatty acid concentration was greatest when 3.5 kg of concentrate was allocated in the AMS and ruminal ammonia decreased linearly with increasing AMS concentrate. Time spent lying, the number of lying bouts, and average bout duration were not affected by treatment. These data indicate that increasing the quantity of concentrate in the AMS increases daily variability in AMS concentrate intake while decreasing PMR intake without affecting voluntary visits to the AMS and milk or milk component yield. As such, under isocaloric dietary settings, increasing the supply of pellet in the AMS is not likely to affect voluntary visits to the AMS, milk and milk component yields, or ruminal fermentation.     

The effect of the forage-to-concentrate ratio of the partial mixed ration and the quantity of concentrate in an automatic milking system for lactating Holstein cows

This study was conducted to evaluate the effects of the forage-to-concentrate ratio of the partial mixed ration (PMR) and the quantity of concentrate offered in an automated milking system (AMS), in a feed-first guided-flow barn, on the behavior and performance of dairy cows. Eight ruminally cannulated multiparous Holstein cows were used in a replicated 4 × 4 Latin square balanced for carry-over effects. Treatments were arranged in a 2 × 2 factorial consisting of a PMR that contained (dry matter basis) either a low (54:46; L-FOR) or a high (64:36; H-FOR) forage-to-concentrate ratio and AMS concentrate provision to achieve low (2 kg/d; L-AMS) or high (6 kg/d; H-AMS) intake. Each period consisted of 28 d with 6 d for dietary transition, 13 d for adaptation, and 9 d of collection. The first 4 d of data and sample collection were used to evaluate behavioral data (milking frequency, feeding behavior, and standing and lying behavior) and ruminal pH. Subsequently, a sampling device removal day was provided, and the last 4 d were used to evaluate ruminal fermentation and apparent total-tract digestibility. All 9 d were used for milk yield measurement, and the 8 d were used for dry matter intake measurement. Cows fed the H-AMS consumed 3.5 kg/d less PMR while consuming 4.2 kg/d more AMS concentrate, but total dry matter intake (PMR+AMS) was not affected by treatments averaging 27.3 kg/d. Although cows fed H-AMS had greater concentrate intake, they also had greater variability for AMS concentrate intake among days (0.85 vs. 0.25 kg/d, respectively). The number of PMR meals and PMR eating behavior were not affected by the PMR or AMS treatments. Feeding H-AMS did not affect milking frequency averaging 3.63 milkings/d, but tended to increase milk yield by 1.25 kg/d relative to L-AMS. Likewise, cows fed the L-FOR tended to have greater milk yield relative to H-FOR (39.3 vs 37.9 kg/d, respectively), but had greater holding area time. Minimum ruminal pH tended to be lower for cows fed L-FOR compared with cows fed H-FOR but was not affected by the AMS concentrate treatment. When fed the L-FOR, feeding the H-AMS increased total short-chain fatty acid concentration in the rumen relative to cows fed L-AMS, whereas the response for H-FOR was not affected by the AMS concentrate. These data suggest that feeding H-AMS may improve milk yield, but also increases the day-to-day variability in AMS concentrate consumption. Feeding a L-FOR PMR may increase milk yield without affecting variability in AMS concentrate consumption; however, it may reduce ruminal pH and increase the time spent in the holding area compared with cows fed a H-FOR PMR.     

Effects of concentrate allowance and individual dairy cow personality traits on behavior and production of dairy cows milked in a free-traffic automated milking system

The primary objective of this study was to determine whether the level of concentrate allowance in an automated milking system (AMS) affects the feed intake, eating behavior, milking activity, and performance of lactating dairy cows. The secondary objective of this study was to describe how the response to concentrate allocation, specifically in feeding and milking behavior, varies with cow personality traits. Fifteen Holstein cows were used in a crossover design with two 28-d periods, each including 14 d of adaptation and 14 d of data collection. The cows were housed in a freestall pen with free-traffic access to the AMS. Treatments consisted of a basal partial mixed ration (PMR) common to both treatment groups, with a concentrate allowance (on dry matter basis) of (1) 3.0 kg/d in the AMS (L-AMS) or (2) 6.0 kg/d in the AMS (H-AMS). Between the 2 treatment periods, each cow was assessed for personality traits using a combined arena test consisting of exposure to a novel environment, novel object, and novel human. Principal component analysis of behaviors observed during the novel environment and object tests revealed 3 factors (interpreted as active, social, and alert-curious) that together explained 76% of the variance, whereas principal component analysis of the novel human test revealed 2 factors (interpreted as active-vocal and fearful of novel humans) that together explained 77% of the variance. When on the H-AMS treatment, PMR dry matter intake (DMI) was less (24.5 vs. 26.0 kg/d) and AMS concentrate delivery was greater (5.9 vs. 3.1 kg/d), as per design. Consequently, total DMI was greater on the H-AMS treatment (30.4 vs. 29.1 kg/d). When on the H-AMS treatment, cows who were more alert-curious consumed more PMR, whereas cows who were more fearful of the novel human were less likely to receive the maximum amount of AMS concentrate available, limiting their total DMI and increasing the day-to-day variability of that intake. Although this was a preliminary study, these data suggest an association between dairy cow personality traits and how cows respond to increased AMS concentrate allowance.     

Effect of amount of concentrate offered in automatic milking systems on milking frequency, feeding behavior, and milk production of dairy cattle consuming high amounts of corn silage

The objective was to evaluate whether the amount of concentrate offered in an automatic milking systems (AMS) would modify milking frequency, feeding behavior, and milk production. One hundred fifteen lactating cows were used in a cross-over design with 2 periods of 90 d each and 2 treatments: low concentrate (LC; up to 3 kg/d of concentrate at the AMS) or high concentrate (HC; up to 8 kg/d of concentrate at the AMS). Cows were evenly distributed in 2 symmetrical pens, each containing 1 AMS and about 50 cows at any given time. All cows received the same total ration (28% corn silage, 1.67 Mcal of net energy for lactation/kg, 16.5% crude protein, DM basis), but a different amount of concentrate from this ration was offered at the AMS depending on treatment. The concentrate at the AMS had the same composition in both treatments. Cows were fetched when time elapsed, because last milking was greater than 12 h. The amount of concentrate offered at the AMS was proportional to the time elapsed since last visit (125 and 333 g/h for LC and HC, respectively). Milk production, total number of daily milkings, number of cows fetched, or number of voluntary milkings were not affected by treatments. The consumption of basal ration was greater in LC than in HC, but this difference was compensated by a greater consumption of concentrate at the AMS in HC than LC cows. Total dry matter intake tended to be lower, therefore, in HC than in LC cows. Eating rate of the basal ration was greater in LC than in HC, but the total amount of time that cows devoted to eat was similar between treatments. Offering high amounts of concentrate to the AMS feeding a basal ration rich in corn silage did not diminish the need for fetching cows and did not increase the number of daily milkings nor milk production.     

Milk yield and milking station visits of primiparous versus multiparous cows on automatic milking system farms in the Upper Midwest United States

The objective of this study was to investigate milk yield and frequency of visits to the milking station of primiparous versus multiparous cows at different stages of lactation on farms with automatic milking systems (AMS) in the Upper Midwest United States. Forty farms were included in the study, and daily AMS software data were collected for 18 mo. For the investigation of milk yield and milking visits, stage of lactation was categorized into 14 periods, 7 d in length for the first 28 d in milk (DIM) and 30 d in length thereafter until 328 DIM. Cow traffic flow to the AMS (free or guided) was included in the model. For the evaluation of failures and refusals, stage of lactation was categorized into 6 periods, 7 d in length each for the first 28 DIM, and 2 periods of 150 d in length each thereafter until 328 DIM. Failures are milking station visits where a cow fails to be milked due to lack of machine attachment although it is time for the cow to be milked. Refusals are milking station visits before adequate time has passed since previous milking, thus the cow leaves the milking station without being milked. Data from lactation days beyond 328 DIM were excluded from the study. Primiparous cows in free-flow systems produced less milk than multiparous cows until the 11th stage of lactation and produced more milk from the 12th stage until the end of the study period. Primiparous cows in guided-flow systems produced less milk than multiparous cows all 14 stages of lactation, but were approaching the milk yield of multiparous cows at the end of the study period. This was a biologically normal lactation curve for primiparous cows. However, estimated peak ratio (primiparous vs. multiparous cows' peak milk yield) was lower than industry standards. Both traffic flow systems had fewer milking visits for primiparous cows compared with multiparous cows in early lactation. This lower milking frequency persisted until the 11th stage of lactation in free-flow systems. In guided-flow systems, primiparous cows were milked less frequently until the 5th stage of lactation, had similar milking frequency in the 6th stage of lactation, and were milked more frequently thereafter. Failures were greater for primiparous cows during all stages of lactation. However, the greatest differences were detected in the early stages of lactation. Primiparous cows had 0.067 more failures/cow per day on average than multiparous cows during wk 1 of lactation. For the remaining lactation stages, differences in failures ranged from 0.003 to 0.039. Refusals were less frequent (0.4 to 0.6/d) for primiparous cows during the first 2 wk of lactation, similar for wk 3 of lactation, and more frequent for the remaining lactation stages (0.10 to 0.14/d). Failures and refusals were only evaluated in free-flow systems. These findings appear to indicate a potential lagging performance for primiparous cows in early lactation as compared with multiparous cows. Additional investigation into improving the adaptation of primiparous cows to AMS in early lactation may be warranted.     

The effects of a training program using a phantom to accustom heifers to the automatic milking system

The introduction of heifers into the automatic milking system (AMS) can be associated with considerable stress for both animals and farm employees, as completely inexperienced heifers initially do not independently enter the unknown milking robot. This study investigated whether training heifers on an AMS phantom provides the possibility of preparing heifers for the following lactation at the AMS. For this purpose, 77 Holstein-Friesian heifers were randomly assigned to one of 2 experimental groups: control (CON) or phantom (PHAN). Four weeks before calving, the PHAN group was given free access to the phantom, which was similar to the actual milking robot, so that they could explore it and be positively conditioned by feeding concentrate in the phantom. The heifers of the CON group had no contact with the phantom or the AMS before the first milking at the AMS. The milking frequency per animal per day was recorded, and the proportion of animals that had to be fetched for milking was determined, to evaluate how the animals accepted the AMS after calving. To assess the stress level of the animals before and after introduction into the AMS, fecal cortisol concentrations and rumination times of the animals were measured. Additionally, lactation performance characteristics (milk yield, milk flow, electrical conductivity of milk, and milk composition) were recorded for 77 animals. The animals trained on the phantom showed a higher milking frequency (DIM 7: 2.70 ± 0.14 visits/d) than the control animals (DIM 7: 2.41 ± 0.14 visits/d) between the 4th and 10th day of lactation. In addition, between d 1 and d 5, the proportion of animals that had to be fetched for milking was lower in PHAN (DIM 1: 35.18 ± 4.16%) than in CON (DIM 1: 48.03 ± 4.46%). The PHAN heifers had unexpectedly high fecal cortisol levels (1 wk prepartum: 43.50 ± 0.93 ng/g of feces), although not considerably elevated compared with CON (1 wk prepartum: 40.76 ± 1.05 ng/g of feces). Training on the phantom had no appreciable influence on rumination time and lactation performance parameters. The increased number of milking visits and the reduced proportion of animals that had to be fetched into the AMS for milking indicate that training on the phantom prepares the animals well for being milked in the AMS. Therefore, training heifers on the phantom offers the possibility to facilitate the start into early lactation for the animals, providing a valuable contribution to improvement of animal welfare.     

Feeding of pellets rich in digestible neutral detergent fiber to lactating cows in an automatic milking system

If the milking frequency in an automatic milking system (AMS) is increased, the intake of concentrated pellets in the robot may be raised accordingly. Consumption of a large quantity of starchy grains within a short time can impair the appetite, decrease voluntary visits to the milking stall, and lower intakes of dry matter (DM) and neutral detergent fiber (NDF). Therefore, the hypothesis to be tested in this study was whether conventional starchy pellets fed in the AMS could be replaced with pellets rich in digestible NDF without impairing the cows’ motivation to visit a milking stall voluntarily. Fifty-four cows were paired according to age, milk yield, and days in milk, and were fed a basic mixture along the feeding lane (19.9 kg of DM/cow per d), plus a pelleted additive (approximately 5.4 kg of DM/cow per d) that they obtained in the milking stall and in the concentrate self-feeder that they could enter only after passing through the milking stall. The 2 feeding regimens differed only in the composition of the pelleted additive, which, for the control group, contained 49% starchy grain, and for the experimental group contained 25% starchy grain plus soy hulls and gluten feed as replacement for part of the grain and other low-digestible, NDF-rich feeds. Both diets resulted in similar rates of voluntary milkings (3.31 vs. 3.39 visits/cow per d). Average yields of milk and percentages of milk protein were also similar in the 2 groups. The results suggest that an alternative pellet composition can be allocated in the AMS in conjunction with basic mixture in the feeding lane, without any negative effect on appetite, milk yield, milk composition, or milking frequency of the cows. It also opens the opportunity to increase yields of milk and milk solids by increasing the amount of pelleted concentrates that can be allocated to selected high-yielding cows via the AMS, because this can be done while maintaining a high frequency of voluntary milkings.     

The effect of two traffic situations on the behavior and performance of cows in an automatic milking system

Two cow traffic situations were tested sequentially in an automatic milking system (AMS) for effects on cow behavior, effective use of the barn, and milking capacity. The first situation was forced cow traffic: 63 cows had to pass through the AMS to go from the lying area to the feeding area. The second was semiforced cow traffic: 67 cows (60 cows from before) had free access to a forage feeding area at one end of the barn but could only access an area with concentrate feeders by passing through the AMS. Behavior of all cows was monitored as well as for two subsets of cows present in both situations: 8 cows with low frequency vs. 7 cows with high frequency of visits to the AMS. In each situation, cows were observed for 72 h. Cow locations and behavior were noted at 10-min intervals for all cows and individually for the selected cows. In semiforced traffic, the herd readily used the freely accessible forage feeding area, ate longer (17.4% of the day vs. 15.1 +/- 0.59%), and stood less in freestalls (9.0 vs. 11.8 +/- 0.30%) than when cow traffic was forced. Nonmilking visits to the AMS tended to decrease, whereas milking visits remained unchanged in the semiforced situation. The subset of cows that visited the AMS more often had fewer nonmilking visits (1.8 vs. 4.2 +/- 0.7) in the semiforced traffic situation whereas cows with low frequency of visits to the AMS had a nonsignificant increase (1.5 vs. 1.0) in nonmilking visits. Cows that visited the AMS frequently used the forage feeding area and the lying area next to it more than low frequency cows and use of those areas increased further during the semiforced situation. The semiforced cow traffic was deemed more desirable than forced cow traffic both for cows and for the capacity of the automatic milking system.     

Performance and feeding behavior of primiparous cows loose housed alone or together with multiparous cows

Lactating Holstein cows (52 multiparous and 90 primiparous) were monitored over a period of 10 mo to observe effects of grouping primiparous cows (PPC) separately from multiparous cows (MPC) on performance, feeding behavior, feed intake, feed efficiency, and milk production of PPC. Cows were kept in 2 symmetrical pens each equipped with a robotic milking unit, 2 waterers, and 28 feeding spaces. Typically, 100 lactating cows were present at a time, thereby ensuring 1.78 cows per feeding place in each pen. One pen (PP) was composed exclusively of PPC whereas the other pen (PM) included 30% PPC and 70% MPC. Primiparous cows were evenly distributed to each pen by days in milk and daily milk production. As they calved, additional primiparous cows were assigned sequentially to each of the 2 treatment groups; multiparous cows calving during the study were allocated to the PM group. Both PP and PM groups were managed equally and were fed the same basal ration twice daily plus 3 kg/d of concentrate during milking. Individual eating behavior and feed consumption at each visit were monitored electronically. Milk production was recorded daily, and milk composition monthly. Observed arithmetic means and standard errors are presented but application to other management situations is limited because animals within pen were not independent. Total dry matter intake (18.7 vs. 18.1 ± 0.9 kg/d) and milk production (25.9 vs. 25.6 ± 0.8 kg/d) of PPC were similar in both the PM and PP groups, respectively. Primiparous cows in the PP group had numerically more visits to the robotic milking unit (3.26 vs. 2.68 ± 0.15) and to the feed troughs (4.91 vs. 4.02 ± 0.43), but apparently spent less time eating (2.72 vs. 3.22 ± 0.1 h/d) than did PPC in the PM group. Differences in feed efficiency were low but PPC in the PP group had numerically higher feed efficiency at times through 200 d in milk. Alternative grouping strategies illustrate potentially important differential responses among primiparous cows that warrant further study.     

Factors associated with productivity on automatic milking system dairy farms in the Upper Midwest United States

The objective of this study was to identify housing and management factors associated with productivity on automatic milking system (AMS) dairy farms measured as daily milk yield/AMS and daily milk yield/cow. Management, housing, and lameness prevalence data were collected from 33 AMS farms in Minnesota and Wisconsin during a farm visit. All farms in the study used free-flow cow traffic. Mixed model analysis of cross-sectional data showed that farms with automatic feed push-up via a robot produced more milk per AMS/day and per cow/day than farms where feed was pushed up manually. New versus retrofitted facility, freestall surface, manure removal system, and the number of AMS units/pen were not associated with daily milk yield per AMS or per cow. Cow comfort index (calculated as number of cows lying down in stalls divided by total number of cows touching a stall) was positively associated with daily milk yield/cow. Prevalence of lameness and severe lameness, number of cows per full-time employee, depth of the area in front of the AMS milking station, and length of the exit lane from the AMS milking station were not associated with daily milk yield per AMS or per cow. Multivariable mixed model analysis of longitudinal AMS software data collected daily over approximately an 18-mo period from 32 of the farms found a positive association between daily milk yield/AMS and average age of the cows, cow milking frequency, cow milking speed, number of cows/AMS, and daily amount of concentrate feed offered/cow in the AMS. Factors negatively associated with daily milk yield/AMS were number of failed and refused cow visits to the AMS, treatment time (the time spent preparing the udder before milking and applying a teat disinfectant after milking), and amount of residual concentrate feed/cow. Similar results were also found for daily milk yield on a per cow basis; however, as it would be expected, average days in milk of the herd were also negatively associated with daily milk yield/cow. These findings indicate that several management and cow factors must be managed well to optimize AMS productivity.     

Short communication: Effects of changing teatcup removal and vacuum settings on milking efficiency of an automatic milking system

The aim of this experiment was to assess strategies to reduce milking time in a pasture-based automatic milking system (AMS). Milking time is an important factor in automatic milking because any reductions in box time can facilitate more milkings per day and hence higher production levels per AMS. This study evaluated 2 end-of-milking criteria treatments (teatcup removal at 30% and 50% of average milk flowrate at the quarter-level), 2 milking system vacuum treatments (static and dynamic, where the milking system vacuum could change during the peak milk flowrate period), and the interaction of these treatment effects on milking time in a Lely Astronaut A4 AMS (Maassluis, the Netherlands). The experiment was carried out at the research facility at Teagasc Moorepark, Cork, Ireland, and used 77 spring-calved cows, which were managed on a grass-based system. Cows were 179 DIM, with an average parity of 3. No significant differences in milk flowrate, milk yield, box time, milking time, or milking interval were found between treatments in this study on cows milked in an AMS on a pasture-based system. Average and peak milk flowrates of 2.15 kg/min and 3.48 kg/min, respectively, were observed during the experiment. Small increases in maximum milk flowrate were detected (+0.09 kg/min) due to the effect of increasing the system vacuum during the peak milk flow period. These small increases in maximum milk flowrate were not sufficient to deliver a significant reduction in milking time or box time. Furthermore, increasing the removal setting from 30% of the average milk flowrate to 50% of the average milk flowrate was not an effective means of reducing box time, because the resultant increase in removal flowrate of 0.12 kg/min was not enough to deliver practical or statistically significant decreases in milking time or box time. Hence, to make significant reductions in milking time, where cows have an average milk flow of 2 kg/min and yield per milking of 10 kg, end-of-milking criteria above 50% of average milk flowrate at the quarter level would be required.     

Reducing prepartum urine pH by supplementing anionic feed ingredients: Effects on physiological and productive responses of Holstein × Gir cows

This study compared physiological and productive parameters in 3/4 Holstein × 1/4 Gir dairy cows receiving a prepartum concentrate containing ammonium chloride to reduce urine pH near 7.0 (CON; n = 17), or a commercial anionic supplement to reduce urine pH near 6.0 (SUPP; n = 17). Nonlactating, multiparous, pregnant cows were assigned to receive SUPP or CON beginning 21 d before expected date of calving. Cows were maintained in a single drylot pen with ad libitum access to corn silage, and individually received their prepartum concentrate once daily (0800 h) before calving. Cows from both treatments completely consumed their concentrate allocation within 30 min after feeding. Cow body weight and body condition score were recorded once weekly, urine pH measured every 3 d, and blood samples collected on d ?21, ?14, ?9, ?6, and ?3 relative to expected calving date. After calving (d 0), cows were moved to an adjacent drylot pen with ad libitum access to water and a total mixed ration, and were milked twice daily (0600 and 1700 h). Cow body weight and body condition score were recorded once weekly and individual milk production was recorded daily until 30 d in milk (DIM). Blood samples were collected before each milking during the first 5 DIM, as well as at 6, 9, 16, 23, and 30 DIM before the morning milking. Based on actual calving dates, cows received SUPP or CON for (mean ± standard error) 19.2 ± 1.2 and 19.0 ± 0.9 d before calving, respectively. Urine pH was less in SUPP versus CON cows during the last 15 d of gestation (6.12 vs. 7.15, respectively). Milk yield during the first 5 DIM and throughout the experimental period was greater in SUPP versus CON cows (by 20 and 14%, respectively), whereas serum Ca concentrations did not differ between treatments during the first 5 DIM. Serum concentrations of fatty acids were greater in SUPP versus CON cows 3 d before and at calving (by 52 and 22%, respectively), whereas SUPP cows had lower serum glucose and cortisol concentration at calving (by 23 and 27%, respectively). Hence, the SUPP treatment decreased prepartum urine pH near 6.0 in Holstein × Gir dairy cows without depressing concentrate intake compared with CON, although total dry matter intake was not evaluated to fully investigate feed intake responses. Moreover, the SUPP treatment transiently affected serum glucose, fatty acids, and cortisol concentrations near the time of calving, and resulted in greater milk yield during the initial 30 DIM compared with CON.     

Cow-level associations of lameness,behavior, and milk yield of cows milked in automated systems

This study evaluated differences in behavior and productivity between lame and nonlame cows in herds with automated milking systems (AMS). We monitored 30 cows per herd on 41 farms with AMS in Canada (26 herds in Ontario and 15 herds in Alberta). During a 6-d period, milking data (n = 1,184) and lying behavior data (n = 1,209) were collected from cows on 41 farms. Rumination behavior (n = 569) and activity (n = 615) data were available for cows at 22 farms. Locomotion was scored using a numerical rating system (NRS; 1 = sound; 5 = extremely lame). Cows were defined as clinically lame with NRS ≥ 3 (n = 353, 29%) and nonlame with NRS < 3 (n = 865, 71%). Greater parity, lower body condition, and lower environmental temperature were factors associated with lameness. When accounting for other factors, lame cows produced 1.6 kg/d less milk in 0.3 fewer milkings/d. Lame cows were 2.2 times more likely to be fetched more than 1 time during the 6-d period and spent 38 min/d more time lying down in bouts that were 3.5 min longer in comparison with nonlame cows. As the number of cows per AMS unit increased, the frequency of milkings and refusals per cow per day decreased and cow activity increased. For each 13.3-percentage-point increase in freestall stocking density (cows per stall), daily lying time decreased by 13 min/d and cows were 1.6 times more likely to be fetched more than 1 time during the 6-d period. There was no difference in daily rumination or activity between lame and nonlame cows or in night:day rumination time, but lame cows had greater night:day activity ratios. This study supports the growing knowledge that lameness has negative effects on milk production, voluntary milking behavior, and lying behavior of cows in herds with AMS. These results may help dairy producers gain a better appreciation of the negative effects of even moderate cases of lameness and may help motivate them to improve their lameness monitoring and treatment protocols.     

Feeding soyhulls to high-yielding dairy cows increased milk production, but not milking frequency, in an automatic milking system

To attract a cow into an automatic milking system (AMS), a certain amount of concentrate pellets is provided while the cow is being milked. If the milking frequency in an AMS is increased, the intake of concentrate pellets might increase accordingly. Replacing conventional starchy pellets with nonstarchy pellets increased milk yield, milk fat, and milk protein and decreased body weight. The hypothesis was that a nonroughage by-product rich in digestible neutral detergent fiber, such as soyhulls and gluten feed, could replace starchy grain in pellets fed in an AMS. Sixty cows were paired by age, milk yield, and days in milk, and were fed a basic mixture ad libitum [16.2 ± 0.35 (mean ± SE) kg of dry matter intake/d per cow] plus a pelleted additive (6 to 14 kg of dry matter/d per cow) that was consumed in the AMS and in a concentrate self-feeder, which could only be entered after passing through the AMS. The 2 feeding regimens differed only in the composition of the pelleted additives: the control group contained 52.9% starchy grain, whereas the experimental group contained 25% starchy grain, plus soyhulls and gluten feed as replacement for part of the grain. Wheat bran in the control ration, a source of fiber with low digestibility, was replaced with more digestible soyhulls and gluten. During the first 60 d in milk, a cow received 10 to 12 kg of concentrate pellets. After 60 DIM, concentrate feed was allocated by milk production: ≤25 kg/d of milk entitled a cow to 2 kg/d of concentrate feed; >25 kg/d of milk entitled a cow to receive 1 kg/d of additional concentrate feed per 5 kg/d of additional milk production, and >60 kg/d of milk entitled a cow to receive 9 kg of concentrate. The concentrate feed was split between the AMS and concentrate self-feeder. The 2 diets resulted in similar frequencies of voluntary milking (3.12 ± 0.03 to 2.65 ± 0.03 visits/d per cow vs. 3.16 ± 0.00 to 2.60 ± 0.01 visits/d per cow). Average milk yields were higher in the experimental group (42.7 ± 0.76 to 39.09 ± 0.33 kg/d per cow vs. 39.69 ± 0.68 to 37.54 ± 0.40 kg/d per cow) and percentages of milk protein (3.02 ± 0.06 to 3.12 ± 0.05% vs. 3.07 ± 0.04 to 3.20 ± 0.04%) and milk fat (3.42 ± 0.17 to 3.44 ± 0.08% vs. 3.38 ± 0.13 to 3.55 ± 0.06%) were similar in the 2 groups. The results suggest that the proposed pellets high in digestible neutral detergent fiber can be allocated via the AMS to selected high-yielding cows without a negative effect on appetite, milk yield, or milk composition while maintaining a high milking frequency.     

Transition cow nutrition and management strategies of dairy herds in the northeastern United States: Part I—Herd description and performance characteristics

Our objective was to describe management and herd characteristics of the transition period on freestall dairy herds in the northeastern United States using an on-farm survey and prospective cohort design. Enrolled herds (n = 72) had a median of 900 milking cows (range: 345–2,900) and a rolling herd average of 12,674 kg (standard deviation ± 1,220 kg), and 87.2% (n = 82/94) of fresh pens were milked at least 3×/d. The prevalence of herds with ≥15% of sampled cows with elevated concentrations of nonesterified fatty acids prepartum (≥0.27 mmol/L, 2–14 d before parturition) and postpartum [primiparous: ≥0.60 mmol/L, multiparous: ≥0.70 mmol/L, 3–14 d in milk (DIM)], β-hydroxybutyrate postpartum (≥1.2 mmol/L, 3–14 DIM), and haptoglobin postpartum (≥1 g/L, 0–12 DIM) was 51%, 51%, 51%, and 57%, respectively. In most herds, cows were moved to a calving pen when showing signs of labor (73.6%; n = 53/72) instead of 0 to 3 d before expected calving (26.4%, n = 19/72). Cows remained in the calving or maternity pen for a median (range) time of 2 (0–24) h after parturition before moving to the next pen. Primiparous cows remained in the first pen moved to after parturition for a longer period than multiparous cows [median (range) days: 12 (1.5–25) vs. 6 (1.5–22)]. Approximately 20% of herds had routine vaccinations administered in the maternity or calving pen, first pen after parturition, or both. Almost all herds (n = 69/72) performed fresh cow health checks; however, only 53% (n = 38/72) locked up all fresh cows daily. More herds housed primiparous and multiparous cows in separate pens during the far-off dry (65.3%; n = 47/72) and high-lactation (81.9%; n = 59/72) periods compared with the close-up dry (31.9%; n = 23/72) and fresh periods (27.8%; n = 20/72). At least half of the pens observed during the far-off dry, close-up dry, and fresh periods had a stocking density <100%. Approximately one-third of pens observed during the far-off dry period had feed pushed up ≤4×/d compared with approximately 15 to 20% of pens observed during the close-up dry, fresh, and high-lactation periods. More than half of the total mixed ration samples acquired from the far-off and close-up dry period visits had greater than the recommended proportion of particles in the 19-mm screen of the Penn State Particle Separator. The results of this observational study illustrated the range of management practices used in freestall herds in this region and lay the groundwork for future hypothesis-driven studies using this sampled population.     

Benchmarking of farms with automated milking systems in Canada and associations with milk production and quality

The objective of this study was to benchmark the herd-level housing and management strategies of automated milking system (AMS) farms across Canada and assess the associations of these herd-level housing factors and management practices with milk production and quality. Canadian AMS farms (n = 197; Western Canada: n = 50, Ontario: n = 77, Quebec: n = 59, Atlantic Canada: n = 11) were each visited once from April to September 2019, and details were collected related to barn design and herd management practices. Milk-recording data for the 6 mo before farm visits were collected. Farms averaged (± standard deviation) 110 ± 102 lactating cows, 2.4 ± 1.9 AMS units/farm, 47.5 ± 14.9 cows/AMS, 36.7 ± 5.0 kg/d of milk, 4.13 ± 0.34% fat, 3.40 ± 0.16% protein, and a herd-average somatic cell count of 186,400 ± 80,800 cells/mL. Farms mainly used freestall housing systems (92.5%), organic bedding substrates (73.6%), and free flow cow traffic systems (87.8%); farms predominantly milked Holsteins (90.4%). Multivariable regression models were used to associate herd-level housing factors and management practices with milk production and quality. At the herd level, feed push-up frequency (mean = 12.8 ± 8.3 times per day) and feed bunk space (mean = 64 ± 21.5 cm/cow) were positively associated with milk yield. Greater milk yield was associated with herds using inorganic (sand) versus organic bedding, milking Holsteins versus non-Holsteins, and using a form of mechanical ventilation versus natural ventilation alone. Milk fat and milk protein content were only associated with breed. Herds with lower somatic cell counts had more frequent alley cleaning (mean = 12.1 ± 7.5 times per day), wider lying alleys (mean = 304.5 ± 40.0 cm), and sand bedding. The results highlight the importance of using sand bedding, using mechanical ventilation, keeping feed pushed up, ensuring alleys are clean, and ensuring adequate space at the feed bunk for maintaining herd-level productivity and milk quality in farms with AMS.     

Effect of automatic milking systems on milk yield in a hot environment

A comparative study was performed to evaluate differences in milk yield between an automatic milking system (AMS) and a conventional herringbone milking parlor system. Two herds of Italian-Friesian cows were reared in the same barn, located in the Po Valley in northern Italy. Twenty-five primiparous cows and 10 multiparous cows were milked with an AMS, while at the same time 29 primiparous and 9 multiparous were milked twice daily in a milking parlor on the other side of the barn. A selection gate allowed cows to access the AMS only if the interval from last milking was >5 h. Multiparous cows in the AMS yielded more milk than multiparous cows in the milking parlor (34.2 ± 0.7 vs. 29.4 ± 0.6 kg/d). There was no difference in milk yield between primiparous cows in the AMS and in the milking parlor (28.9 ± 0.4 vs. 28.8 ± 0.3 kg/d). Milking frequency in the AMS was significantly higher in primiparous (2.8 ± 0.03) than in multiparous cows (2.5 ± 0.04). The hot season negatively affected milk yield; the milk yield reduction was higher for cows milked with the AMS (−4.5 ± 0.6 kg/d) than in the milking parlor (−3.0 ± 0.8 kg/d). In the AMS, milking frequency decreased during the hot season in primiparous cows (−0.3 ± 0.1). We concluded that a positive AMS effect on milk yield is possible, but that steps must be taken to alleviate the discomfort involved with attracting cows to the AMS.     

Effect of increased milking frequency in early lactation with or without recombinant bovine somatotropin

Multiparous Holstein cows (n = 300) were assigned to 1 of 2 milking frequency treatments at parturition. Cows were either milked 6 times (6×) or 3 times (3×) daily to determine effects on early lactation milk yields and subsequent lactation persistency with or without use of recombinant bST (rbST). Treatments included a control group milked 3× and 3 groups milked 6× for either the first 7, 14, or 21 days in milk (DIM). Those 4 groups of cows all received rbST starting at 63 DIM. The fifth treatment group was also milked 6× for the first 21 DIM but those cows received no rbST during the entire lactation. All cows returned to 3× milking after their respective treatment periods ended. Cows milked 3× tended to produce more milk (43.2 vs. 41.5 and 41.0 ± 1.1 kg/d) during the first 9 wk of lactation compared with cows milked 6× for 7 or 21 DIM, respectively. Group milk yields after wk 9 averaged 38.3 ± 0.7 kg/d and did not differ among various groups assigned to an increased milking frequency in early lactation. Percentages of milk fat (3.8 ± 0.12%) and protein (2.9 ± 0.06%) did not differ among treatments during the first 9 wk after calving. Early lactation milk yield (41.9 ± 1.2 kg/d) did not differ between the 2 groups of cows milked 6× for 21 DIM. However, cows subsequently administered rbST (at 63 DIM) produced more milk (38.8 vs. 34.2 ± 0.9 kg/d) from wk 10 to 44. The number of cows sent to the hospital during the 305-d trial for mastitis (97), digestive disorders (14), respiratory issues (9), lameness (22), or retained placenta (16), were not affected by treatments (χ² = 0.49). Under the conditions of this commercial dairy herd in Arizona, increasing milking frequency to 6 times daily for 7 to 21 d at the start of lactation conditions did not increase milk yield nor improve lactation persistency.     

Effect of dry-off management on milking behavior,milk yield,and somatic cell count of dairy cows milked in automated milking systems

Milk production may be reduced before dry-off to decrease the risk of cows developing intramammary infections during the dry period. Such reductions in milk may be possible in automated milking systems (AMS) where milking frequency and feed allocation at the AMS can be controlled at the cow level. This study investigated the effect of dry-off management of cows milked in AMS on milk yield, milking behavior, and somatic cell count (SCC). Using a 2 × 2 factorial arrangement of treatments, applied from d 14 to 1 before dry-off, 445 cows from 5 commercial dairy farms in Quebec, Canada, were assigned within farm to either (1) reduced feed [RF; allowed a maximum of 0.75 kg/d of AMS pellet for the first week (14 to 8 d before dry-off) of treatment, and 0.50 kg/d for the second week (7 to 1 d before dry-off) of treatment], or (2) nonreduced feed (NF; allowed up to 2 kg/d of AMS pellet), and either (1) reduced milking (RM; reduced to 2 milkings/d or as many times as required to yield 17 kg/milking), or (2) nonreduced milking (NM; allowed up to 6 AMS milkings/d) and no maximum production. Feed and milking behavior data, as well as milk yield and SCC were collected from the AMS software. The RF cows had lower AMS feed delivered during the treatment period, as per the experimental design. Across the treatment period, the NF-NM cows had the highest milking frequency (2.7 times/d), followed by the RF-NM cows (2.4 times/d), and then both of the RM groups (1.8 times/d), which did not differ from each other. All cows, except the NF-NM cows, were gradually milked less frequently as dry-off approached. Across the entire 2-wk treatment period before dry-off, cows with RM allowance experienced a higher reduction in milk yield compared with the cows with no milking allowance restrictions (?4.8 vs. ?3.6 kg). Similarly, cows with a RF allocation tended to have a higher reduction in milk yield than cows with NF (?4.6 vs. ?3.7 kg). As result, those cows with both reduced milking permissions and feed allocation at the AMS experienced the greatest drop in milk production before dry-off. There were no differences between treatments for milking frequency or yield in the next lactation. Somatic cell score (calculated from SCC) was not different between treatments in the 2-wk or day before dry-off, nor in the first month after calving. Overall, these data suggest that reducing both milking frequency and feed quantity in the AMS is the most efficient method to decrease milk yield before dry-off, without negatively influencing milking frequency or yield in the next lactation, as well as without affecting milk quality.