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《光学仪器》2017,(1)
"衍射极限"实际上不是一个真正的障碍,除非处理远场和定位精度。这种衍射障碍并不是坚不可摧的,可以利用一些智能技术来突破光学衍射极限。讨论了四种技术,近场扫描光学显微镜(NSOM)法,受激发射损耗(STED)显微镜法,光激活定位显微镜(PALM)法或随机光学重建显微镜(STORM)法和结构照明显微镜(SIM)法,并且介绍了各自的基本原则与优劣。NSOM利用纳米级探测器检测通过光纤的极小汇聚光斑,从而获得单个像素的分辨率;PALM和STORM利用荧光探针,实现暗场和荧光的转换,从而观察到极小的荧光团;SIM则是利用栅格图案与样品叠加成像来实现。其中,STORM具有相对较高的潜力,能够更为有效地突破衍射极限。 相似文献
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概述了光学宽视场显微镜、共聚焦显微镜、超分辨率显微镜中所应用的现代显微成像技术,对各种传统和先进的显微成像原理进行了总结。光学宽视场显微镜最常用的显微技术有明场成像、暗场成像、相衬成像、偏光成像、微分干涉(DIC)成像、调制对比成像和荧光成像。相衬成像中根据不同的成像结构还有切趾相衬成像。微分干涉除了传统的偏振光照明还有圆偏振光照明(C-DIC)和专用于塑料的微分干涉(PlasDIC)。共聚焦显微镜随着计算机技术和制造技术的发展而有了巨大的发展。除了传统的共聚焦荧光显微镜以外,还有连续反斯托克斯拉曼散射(CARS)共聚焦、多光子共聚焦和白光共聚焦。超分辨率显微镜中主要介绍了受激辐射淬灭(STED)技术和紧随基态淬灭显微技术的单分子返回(GSDIM)技术。 相似文献
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红外热成像技术是现代影像学中的一门新兴技术。它与x射线、B超、CT、核磁共振等显像技术的成像原理不同,它不主动发射任何射线,只是被动接受热源所发射出的红外线,经过处理后得到热源的影像。该技术的最大特点是不用接触待测物体。因此,对于一些高危行业,如核工业中元器件的检测将变得非常容易。本文所叙述的就是利用红外热像技术与显微技术的结合,制作一种红外显微镜。红外显微镜可以将出现故障的大规模集成电路板中数以万计的微小元器件的影像传输到计算机中,经过计算机的分析,可以很容易地分析出具体故障所在。因此,大范同电子元器件故障的快速检测将变得简单、快捷。 相似文献
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R. Kompfner C. J. R. Sheppard D. Walsh A. Choudhury J. N. Gannaway P. G. Hale 《Scanning》1994,16(6):327-332
The following consists of extracts concerning near-field microscopy from a Progress Report prepared in April 1975 as Oxford University Engineering Laboratory Report No. 1883/77, describing work initiated by the late Professor R. Kompfner. Preliminary experimental work on near-field microscopy is outlined, and a theory for contrast formation is presented. 相似文献
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R. Kompfner C. J. R. Sheppard D. Walsh A. Choudhury J. N. Gannaway P. G. Hale 《Scanning》1994,16(3):327-332
The following consists of extracts concerning near-field microscopy from a Progress Report prepared in April 1975 as Oxford University Engineering Laboratory Report No. 1883/77, describing work initiated by the late Professor R. Kompfner. Preliminary experimental work on near-field microscopy is outlined, and a theory for contrast formation is presented. 相似文献
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Many different optical schemes for achieving super-resolution by probing a near field have been proposed and one of the most important of these is the use of a tapered optical fibre probe. Plain optical fibre probes have also been used for microscopy. The Fourier optics behind these various methods are considered, contrast mechanisms discussed, and the concept of the transfer function for an evanescent field developed. 相似文献
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We investigate the ability of the bow‐tie slot antenna to generate intense optical spots below the diffraction limit. A commercially available finite‐difference time‐domain electromagnetic modelling software is used in the numerical simulations. The finite‐difference time‐domain software is first compared to analytical results at optical frequencies to verify its accuracy. We then present numerical simulations for various geometries involving apertures on thin films and the bow‐tie antenna. The transmission efficiency and optical spot size of the bow‐tie antenna are compared with those of rectangular and circular apertures on thin metal films. We also investigate the effects of material composition, frequency, and antenna geometry on the near‐field radiation pattern using numerical simulations. 相似文献
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A novel technique for scanning near‐field optical microscopy capable of point‐contact current‐sensing was developed in order to investigate the nanometre‐scale optical and electrical properties of electrochromic materials. An apertureless bent‐metal probe was fabricated in order to detect optical and current signals at a local point on the electrochromic films. The near‐field optical properties could be observed using the local field enhancement effect generated at the edge of the metal probe under p‐polarized laser illumination. With regard to electrical properties, current signal could be detected with the metal probe connected to a high‐sensitive current amplifier. Using the current‐sensing scanning near‐field optical microscopy, the surface topography, optical and current images of coloured WO3 thin films were observed simultaneously. Furthermore, nanometre‐scale electrochromic modification of local bleaching could be performed using the current‐sensing scanning near‐field optical microscopy. The current‐sensing scanning near‐field optical microscopy has potential use in various fields of nanometre‐scale optoelectronics. 相似文献
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H. U. Danzebrink TH. Dziomba T. Sulzbach O. Ohlsson C. Lehrer & L. Frey 《Journal of microscopy》1999,194(2-3):335-339
The near-field probes described in this paper are based on metallized non-contact atomic force microscope cantilevers made of silicon. For application in high-resolution near-field optical/infrared microscopy, we use aperture probes with the aperture being fabricated by focused ion beams. This technique allows us to create apertures of sub-wavelength dimensions with different geometries. In this paper we present the use of slit-shaped apertures which show a polarization-dependent transmission efficiency and a lateral resolution of < 100 nm at a wavelength of 1064 nm. As a test sample to characterize the near-field probes we investigated gold/palladium structures, deposited on an ultrathin chromium sublayer on a silicon wafer, in constant-height mode. 相似文献
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H. Kawashima M. Furuki† ‡ S. Tatsuura† M. Tian‡ Y. Sato‡ L. S. Pu‡ & T. Tani§ 《Journal of microscopy》2001,202(1):241-243
A near-field scanning optical microscope has been combined with a two-colour time-resolved pump-probe measurement system. It has a noise-equivalent transmittance change of 5.0 × 10−5 for a probe pulse with an intensity of 30 nW. The system has been used for evaluating molecular thin films that have a domain structure, particularly for observing a gate action of the single domains. The results include key features to understand an origin of the domains and suggest that the film composition is uniform over a distance of several micrometres. 相似文献
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H.-J. Maas J. Heimel H. Fuchs U. C. Fischer J. C. Weeber† & A. Dereux† 《Journal of microscopy》2003,209(3):241-248
Scanning near‐field optical microscopy images of metal nanostructures taken with the tetrahedral tip (T‐tip) show a distribution of dark and bright spots at distances in the order of 25–50 nm. The images are interpreted as photonic nanopatterns defined as calculated scanning near‐field optical microscopy images using a dipole serving as a light‐emitting scanning near‐field optical microscopy probe. Changing from a positive to a negative value of the dielectric function of a sample leads to the partition of one spot into several spots in the photonic nanopatterns, indicating the excitation of surface plasmons of a wavelength in the order of 50–100 nm in metal nanostructures. 相似文献