Calibration curves for biological dosimetry by fluorescence in situ hybridisation

Dose-response curves were measured for the induction of chromosomal aberrations in peripheral blood lymphocytes after acute exposure in vitro to 60Co gamma rays. Blood was obtained from four different healthy donors, and chromosomes were either observed at metaphase, following colcemid accumulation, or prematurely condensed by calyculin A. Cells were analysed in three different Italian laboratories. Chromosomes 1, 2, and 4 were painted, and simple-type interchanges between painted and non-painted chromosomes were scored in cells exposed in the dose range 0.1-3.0 Gy. The chemical-induced premature chromosome condensation method was also used combined with chromosome painting (chromosome 4 only) to determine calibration curves for high dose exposures (up to 20 Gy X rays). Calibration curves described in this paper will be used in our laboratories for biological dosimetry by fluorescence in situ hybridisation.     

Investigation of Teflon electret detectors for beta dosimetry

Cylindrical electret ionization-chamber-type dosimeters exposed to beta radiation show a partial decay of their polarization. Different radiation field geometries and wall materials for the outer jacket were tested.     

A new algorithm for identifying abnormal glow curves in thermoluminescence personal dosimetry

In this paper an algorithm for the investigation of routine curves in thermoluminescence personal dosimetry is presented. In rare cases, the luminescence glow curves do not exhibit the expected form. They have an abnormal shape as a result of, for example, external contamination, hardware problems, and poor heat transfer. So, glow curves from a monthly exposure period are compared with regular glow curves. Each curve is divided into four regions of interest (ROIs) and the relationships between the different ROIs are analysed. There are few criteria combining all four ROIs, which are necessary to distinguish between normal and abnormal glow curves. For that, the numerical value and the channel of the curves maximum also need to be considered. In most cases an additional set of criteria permits the identification of the ROI in which the irregularity occurs.     

CABAS: a freely available PC program for fitting calibration curves in chromosome aberration dosimetry

The aim of biological dosimetry is to estimate the dose and the associated uncertainty to which an accident victim was exposed. This process requires the use of the maximum-likelihood method for fitting a calibration curve, a procedure that is not implemented in most statistical computer programs. Several laboratories have produced their own programs, but these are frequently not user-friendly and not available to outside users. We developed a software for fitting a linear-quadratic dose-response relationship by the method of maximum-likelihood and for estimating a dose from the number of aberrations observed. The program called as CABAS consists of the main curve-fitting and dose estimating module and modules for calculating the dose in cases of partial body exposure, for estimating the minimum number of cells necessary to detect a given dose of radiation and for calculating the dose in the case of a protracted exposure. The program is freely available at http://www.pu.kielce.pl/ibiol/cabas.     

Determination of stress-strain curves of vapour-deposited films

The strength properties of three vapour-deposited silver films, prepared with different thicknesses and crystal structures, have been compared by mounting them on electron microscope grids and then indenting them with a fine tipped stylus. The method is very rapid and enables specimens to be transferred to an electron microscope for microstructural studies, quickly and without risk of “handling” damage. A simple model of the indentation situation is proposed and this is used to calculate stress-strain curves for the films. Although the technique seems unsuitable for strains below ～ 1%, very large strains can be reached, even when work-softening occurs. The results are consistent with those obtained on similar specimens by other investigators.     

Experimental validation of Monte Carlo depth-dose calculations using radiochromic dye film dosimetry for a beta-gamma 153Sm radionuclide applied to the treatment of rheumatoid arthritis

In this work we compare the Monte Carlo (MCNP4B) calculated beta-gamma depth-dose profile for a liquid 153Sm beta-gamma source used in radiation synovectomy with the experimental depth-dose distribution obtained using radiochromic dye film dosimetry. The calculated and experimental depth-dose distribution shows a very good agreement (within 5%) in the region where the dose deposition is dominated by the beta particle component (first 800 microm depth on tissue-equivalent material). The agreement worsens, reaching a maximum deviation of 15%, at depths close to the maximum range of the beta particles. Finally the agreement improves for the region where the gamma component accounts for one-third of the total absorbed dose (depths >1 mm). The possible contributions to these differences are discussed, as well as their relevance for the application of 153Sm in the treatment of rheumatoid arthritis.     

Optically stimulated luminescence dosimetry performance of natural Brazilian topaz exposed to beta radiation

Optically stimulated luminescence (OSL) has become the technique of choice in many areas of dosimetry. Natural materials like topaz are available in large quantities in Brazil and other countries. They have been studied to investigate the possibility of use its thermoluminescence (TL) properties for dosimetric applications. In this work, we investigate the possibility of utilising the OSL properties of natural Brazilian topaz in dosimetry. Bulk topaz samples were exposed to doses up to 100 Gy of beta radiation and the integrated OSL as a function of the dose showed linear behaviour. The fading occurs in the first 20 min after irradiation but it is <6% of the integrated OSL measured shortly after exposure. We conclude that natural colourless topaz is a very suitable phosphor for OSL dosimetry.     

The application of computerised analysis of glow curves to personal dosimetry in LiF:Mg,Cu,P

In personnel monitoring services, it is important to omit the high-temperature annealing process so that large numbers of TL detectors can be produced economically. There are two efficient ways of reducing the residual signal of LiF:Mg,Cu,P. One is by increasing the maximum readout temperature and the other is by improving the preparation procedure (increasing the Cu concentration and the sintering temperature) but both reduce the TL sensitivity. In personal dosimetry the real dosimetric signals are separated from the residual signals by computerised analysis of glow curves. The adverse influence of the high residual signals of LiF:Mg.Cu.P TL material has been effectively eliminated and the sensitivity remains stable. A good dosimetric result using only reader measurement without pre-irradiation oven annealing is attained in a dose range of 50-80,000 microGy.     

Determination of the liquidus-solidus curves for the system PbTe-GeTe

The liquidus and solidus curves for the PbTe-GeTe system were determined by DTA over the entire compositional range. The samples were annealed for one month at 600° C and rapidly quenched. X-ray data for the annealed-quenched samples indicated that solid solution exists across the entire compositional range. Unannealed samples indicated the presence of different phases.     

Determination of quality parameters from statistical analysis of routine TLD dosimetry data

Following the as low as reasonably achievable (ALARA) practice, there is a need to measure very low doses, of the same order of magnitude as the natural background, and the limits of detection of the dosimetry systems. The different contributions of the background signals to the total zero dose reading of thermoluminescence dosemeter (TLD) cards were analysed by using the common basic definitions of statistical indicators: the critical level (L(C)), the detection limit (L(D)) and the determination limit (L(Q)). These key statistical parameters for the system operated at NRC-Negev were quantified, based on the history of readings of the calibration cards in use. The electronic noise seems to play a minor role, but the reading of the Teflon coating (without the presence of a TLD crystal) gave a significant contribution.     

Determination of the ac magnetization curves of ferromagnetic flat products

Various problems involved in determining the ac magnetic properties of solid flat products (plate, strip) over a wide range of magnetic field strength are considered. Relations are obtained for the actual induction and the systematic error. With known and calculated parameters of specimens, the relations can be used to determine the critical frequencies of field variation, below which the measured magnetization curve can be made more precise and the actual magnetization curve can be employed. Translated from Izmeritel'naya Tekhnika, No. 5, pp. 43–45, May, 1995.     

Direct ion storage dosimetry systems for photon, beta and neutron radiation with instant readout capabilities

The direct ion storage (DIS) dosemeter is a new type of electronic dosemeter from which the dose information for both Hp(10) and Hp(0.07) can be obtained instantly at the workplace by using an electronic reader unit. The number of readouts is unlimited and the stored information is not affected by the readout procedure. The accumulated dose can also be electronically reset by authorised personnel. The DIS dosemeter represents a potential alternative for replacing the existing film and thermoluminescence dosemeters (TLDs) used in occupational monitoring due to its ease of use and low operating costs. The standard version for normal photon and beta dosimetry, as well as a developmental version for neutron dosimetry, have been characterised in several field studies. Two new small size variations are also introduced, including a contactless readout device and a militarised version optimised for field use.     

Determination of S–N curves with the application of artificial neural networks

The present paper describes the application of artificial neural networks for estimating the finite-life fatigue strength and fatigue limit. A comprehensive database with results of single-stage tests on specimens which simulate structural components is evaluated and prepared for processing with the use of neural networks. The available data are subdivided into different classes. A total of six different data classes are specified. The results of the prediction by means of neural networks are superior to those obtained with conventional methods for calculating the fatigue strength. The experimental results are estimated with high accuracy.     

Review of patient dosimetry in cardiology

The objective of the study is to review doses to patients undergoing interventional cardiology procedures, doses that are known to be substantial due to prolonged fluoroscopy time and multiple radiographic exposures. High doses together with the fact that the use of radiation in cardiology is increasing, makes the assessment of patient dose and the establishment of reference dose levels necessary. Thus MED 97 can be better implemented in all EU member countries. The literature review has shown that data is limited and difficult to compare due to various dose indicators, different physical parameters of X ray equipment, various examination protocols and variations in patients' size and complexity of case. For these reasons, European countries participating in DIMOND II submitted data for percutaneous transluminal coronary angioplasty and coronary angiography. Reference dose levels were defined in terms of dose-area product, fluoroscopic time and number of frames.     

International intercomparison for criticality dosimetry: the case of biological dosimetry

The Institute of Radiation Protection and Nuclear Safety (IRSN) organized a biological dosimetry international intercomparison with the purpose of comparing (i) dicentrics yield produced in human lymphocytes; (ii) the gamma and neutron dose estimate according to the corresponding laboratory calibration curve. The experimental reactor SILENE was used with different configurations: bare source 4 Gy, lead shield 1 and 2 Gy and a 60Co source 2 Gy. An increasing variation of dicentric yield per cell was observed between participants when there were more damages in the samples. Doses were derived from the observed dicentric rates according to the dose-effect relationship provided by each laboratory. Differences in dicentric rate values are more important than those in the corresponding dose values. The doses obtained by the participants were found to be in agreement with the given physical dose within 20%. The evaluation of the respective gamma and neutron dose was achieved only by four laboratories, with some small variations among them.     

Staff dosimetry in interventional cardiology

In interventional cardiology (IC) staff can be exposed to high dose levels due to the long fluoroscopy procedures performed. Staff dosimetry can yield information on the optimisation level of radiation protection, which is influenced by the equipment performance, auxiliary protection devices, training in radiation protection and procedure complexity. Staff exposure data assessed in haemodynamic laboratories of four hospitals in Spain, Greece and Italy participating in the DIMOND concerted action are analysed and compared with data in the literature.     

Target cells in internal dosimetry

Data related to radium induced bone sarcomas in humans are used as a method of defining target cells on bone surfaces and in the bone marrow. The differential distribution of radiation induced bone sarcoma types, with a high ratio of non-bone producing, mainly fibroblastic tumours, challenges the ICRP concept that the bone lining cells are target cells. Multipotential mesenchymal stem cells are located within the range of alpha particles and are the most likely target cells for the fibroblastic type of bone sarcoma. The histogenesis of bone sarcomas after irradiation with alpha emitters shows that their final histopathology is not dependent on a single target cell. Each target cell has a microenvironment, which has to be regarded as a synergistic morpho-functional tissue unit. For this the concept of 'histion', a term used in general pathology, is proposed. Interactions between target cells that have been hit by alpha particles, leading to lethal, mutational or transformation events with all components of a 'histion', will prove critical to understanding the pathogenesis of both deterministic and stochastic late effects.