荧光定量免疫层析法测定鸡肉和牛奶中的氯霉素

目的建立一种荧光定量免疫层析法测定动物源性食品鸡肉与牛奶中氯霉素残留的分析方法。方法将氯霉素鼠单克隆抗体与羧基化铕微球进行偶联标记。经过荧光微球活化、探针偶联等一系列条件优化确定最佳反应体系条件,根据纸条测试线(T线)信号值与质控线(C线)信号值的比值(T/C)和样本中氯霉素浓度建立定量标准曲线。针对鸡肉和牛奶样品,对荧光免疫层析检测方法的灵敏度、准确度和精密度等进行评价,并与液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)标准仪器分析方法进行比较。结果本研究建立的荧光免疫层析检测方法对鸡肉和牛奶中氯霉素残留的检出限分别为0.041μg/kg和0.040μg/kg,定量限分别为0.079和0.100μg/kg,添加回收率在76.26%～112.92%之间,变异系数均小于15%。结论本研究建立的荧光定量免疫层析方法灵敏度高、稳定性好,且与使用仪器方法得到的检测结果一致性较高,适用于鸡肉和牛奶中氯霉素残留的现场快速检测。     

水产品中氯霉素时间分辨荧光免疫层析定量检测方法

该研究旨在建立一种简单、快速、高灵敏的鱼、虾和蟹中氯霉素残留的荧光定量免疫层析检测方法,以满足水产品中氯霉素残留检测的需求。通过将氯霉素鼠单克隆抗体G375-5与羧基化铕微球进行偶联标记,氯霉素抗原和羊抗鼠二抗分别包被于硝酸纤维素膜(NC)上作为检测线(T)和控制线(C);根据T线信号值与C线信号值的比值(T/C)和样本中氯霉素浓度建立定量标准曲线;针对鱼、虾、蟹样品,对荧光免疫层析检测方法的灵敏度、准确度和精密度等进行评价,并将其在实际水产样品中与HPLC法进行结果对比。结果表明,该研究建立的时间分辨荧光免疫层析检测方法对水产品中氯霉素的定量限为0.1μg/kg,添加回收率为73.5%～114.2%,变异系数3.9%～11.5%,与国标仪器方法检测结果的一致性较高。因此,该研究所建立的时间分辨荧光免疫层析检测方法在水产品氯霉素的快速检测工作中具有较高的应用价值和应用前景。     

时间分辨荧光免疫层析法定量检测粮谷中重金属铅的研究

本研究制备了一种基于竞争抑制原理的粮谷物中铅快速检测的时间分辨荧光免疫层析定量检测卡。采用铕纳米微球作为荧光探针,标记抗Pb-EDTA的单克隆抗体,从而建立了免疫层析定量方法并对其进行方法学研究。结果表明:定量检测卡的灵敏度为0.61 ng/mL,标曲线性范围为1～16 ng/mL,检出限和定量限分别为8.70μg/kg和22.39μg/kg,对有证标准物质的检测回收率在93.2%～104.0%。该法精密度较好,变异系数在10%以内。结果显示,该法灵敏度高、操作简便、成本低廉,因此,非常适合基层单位,便于大量样本的快速初步筛查。     

量子点微球荧光免疫层析定量检测水产、畜禽肉组织中恩诺沙星

目的 建立一种快速定量检测水产、畜禽肉组织中恩诺沙星的新方法学。方法 以量子点荧光微球为新型标记探针,通过参数优化,制备获得量子点荧光微球免疫层析试纸条;进一步以T/C比值法建立定量检测恩诺沙星的新方法。结果 在最优条件下,该试纸条检测缓冲液中恩诺沙星半数抑制浓度为1.035 ng/mL,最低检测限为0.125 ng/mL;检测水产、畜禽肉组织中恩诺沙星的定量检测范围为8.75~560 μg/kg,半数抑制浓度为72.45 μg/kg,加标回收实验显示该方法定量检测组织样本中恩诺沙星的回收率在83.03~124%之间,变异系数小于15%,检测15个实际含有恩诺沙星的水产以及畜禽肉组织样本,其结果与LC/MS/MS方法检测结果高度一致;此外,加速保存实验结果显示该试纸条在室温下可保存一年。结论 本研究以量子点荧光微球为新型标记探针,构建了高灵敏定量检测恩诺沙星的新方法,实现了水产、畜禽肉组织中恩诺沙星的简单、快速、定量分析。     

量子点荧光微球免疫法定量检测小麦中黄曲霉毒素B1

摘 要: 目的 建立量子点荧光微球免疫法快速检测小麦中黄曲霉毒素B1的方法。方法 采用量子点荧光微球作为荧光标记物,与黄曲霉毒素B1的单克隆抗体偶联,构建量子点荧光微球探针。优化缓冲液pH、抗体最小标记量、荧光探针用量和包被抗原浓度等实验条件,建立检测卡上T线和C线信号峰值面积的比值与样本中黄曲霉毒素B1浓度的关系,构建定量标准曲线。针对小麦样品,将该检测方法与时间分辨荧光定量检测方法进行比较。结果 本研究建立的荧光定量免疫层析检测方法最佳反应条件为：pH 7.5磷酸钠缓冲液,抗体标记量为20 μg,荧光探针用量为4.0 μL,抗原质量浓度使用0.40 mg/mL。小麦中黄曲霉毒素B1的定量检测线性范围为0.05 μg/kg-25 μg/kg,其线性拟合方程为Y= -0.6058X + 12.523（r2=0.9994）,检出限为0.02 μg/kg,定量限为0.05 μg/kg。加标回收率在91.50%~115.00%之间,变异系数在1.88%~4.35%之间。结论 本研究建立的荧光定量免疫层析方法快速、准确、稳定性好、可靠性高,适用于小麦中黄曲霉毒素B1的现场快速检测。     

水产品孔雀石绿和隐性孔雀石绿快速定量检测研究

采用胶体金免疫层析方法建立了水产品中孔雀石绿和隐性孔雀石绿的定量检测,并对其样品前处理方法进行了研究。结果表明,孔雀石绿的线性范围为0.25μg/L~8μg/L,线性系数0.996 1;优化后孔雀石绿提取液的pH为4.5,对甲苯磺酸浓度为0.06 mol/L,净化剂为2 g中性氧化铝,脱水剂为2 g氯化钙,氧化剂为100μL 0.1%四氯苯醌;孔雀石绿加标回收率为80.1%~89.6%,隐性孔雀石绿加标回收率为66.9%~72.9%,相对标准偏差1.07%~8.14%。     

时间分辨荧光免疫层析法定量检测稻谷中的镉

本研究制备了一种用于稻谷中镉检测的时间分辨荧光免疫层析定量检测卡。采用铕纳米微球作为荧光探针,标记抗Cd-EDTA的单克隆抗体,基于竞争抑制原理建立免疫层析定量方法并对其进行方法学研究。结果表明：定量检测卡的灵敏度为0.088 ng/mL,标准曲线线性范围为0.1ng/mL～5.0 ng/mL,检出限和定量限分别为0.026 mg/kg和0.064 mg/kg,对有证标准物质的正确度在81.0%~91.7%之间,对实际样本的检测与ICP-MS检测结果一致性较高。该法重复性较好,变异系数在10%以内。结果显示,该法灵敏度高、操作简便、成本低廉,因此,非常适合基层单位大量样本的快速初步筛查。     

荧光定量法快速检测鲜奶中四环素类总量残留

以荧光纳米微球标记抗体,建立免疫层析检测方法,配合荧光定量仪,可高灵敏度地检测样本中4种四环素类药物的残留含量,实现鲜奶中所含四环素类药物的总量检测。结果表明,本方法对四环素的检测下限达10μg/L,对金霉素、土霉素、多西环素回收率均达70%以上,除了与链霉素有0.32%的交叉反应率外,与其他药物没有交叉反应,整个检测过程只需15 min左右。通过建立的荧光定量免疫层析法,实现了四环素总量的快速定量检测。     

胶体金免疫层析法检测水产品中孔雀石绿总量残留

目的建立胶体金免疫层析法快速检测水产品中孔雀石绿总量的残留。方法用柠檬酸三钠还原法制备胶体金并标记孔雀石绿单克隆抗体。基于竞争抑制反应原理,在优化条件下建立胶体金免疫层析法。结果水产品中孔雀石绿总量的检测限为2μg/mL,与结构类似物结晶紫的交叉反应不明显。实际样本检测结果与液相色谱-串联质谱法检测结果的差异较小。结论该方法快速、有效,可满足现场检测水产品中隐性孔雀石绿及孔雀石绿的残留。     

荧光微球免疫层析方法定量检测水产品中4 种硝基呋喃代谢物

采用荧光微球标记抗体作为信号探针,建立荧光微球免疫层析检测方法（fluorescent microsphere immunochromatographic assays,FMICA）,并应用于水产品中4 种硝基呋喃类兽药代谢物残留量的快速定量检测。结果表明：呋喃妥因、呋喃它酮、呋喃唑酮、呋喃西林代谢物FMICA试纸条的检出限分别为0.004（以1-氨基-乙内酰脲衍生物计算）、0.01（以5-吗啉-3-氨基-2-恶唑烷酮衍生物计算）、0.008（以3-氨基-2-恶唑烷酮衍生物计算）、0.009（以氨基脲衍生物计算）μg/L;检测线性范围分别为0.005～5、0.01～10、0.01～10、0.01～10 μg/L;最佳检测时间为10 min,方法特异性良好。水产样品经衍生化处理后,经检测硝基呋喃代谢物的加标回收率在75.88%～104.81%之间,变异系数均小于15%。FMICA方法操作简单、快速、成本低,适用于水产品中硝基呋喃类兽药代谢物快速定量检测。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press