响应面分析法优化艾叶多糖提取工艺研究

利用响应面法对艾叶多糖的提取工艺进行优化。在单因素试验基础上选取试验因素与水平,根据中心组合(Box-Benhnken)试验设计原理采用三因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因素,以艾叶多糖提取率为响应值作响应面和等高线。在分析各个因素的显著性和交互作用后,得出艾叶多糖浸提的最佳工艺条件为：料水比1:18、浸提温度97℃、浸提时间1.25h、浸提3 次,艾叶多糖的实际一次提取率可达1.529%。     

响应面法优化西藏金耳多糖提取工艺研究

试验采取传统的热水浸提法,探究料液比、浸提时间、浸提温度等3个因素对金耳多糖提取率的影响。采用响应面法对金耳多糖的提取条件进行优化,研究结果发现料液比对金耳多糖提取率的影响最大,料液比与浸提温度的交互效应对金耳多糖提取率具有显著影响。确定金耳多糖提取的最佳工艺为:料液比为1∶41(g/m L),提取温度为60℃,提取时间为3 h。在上述条件下得到的西藏野生金耳粗多糖的提取率是7.9%,与响应面模型的预测值相符合,表明利用响应面法优化西藏金耳多糖的热水浸提工艺是可行的。     

山药多糖提取工艺的优化

对山药多糖的提取工艺进行优化.在单因素试验基础上选取试验因素与水平,利用响应面法(RSM),根据Box-Benhnken中心组合试验设计原理,采用三因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以山药多糖提取率为响应值作响应面和等高线.在分析各个因素的显著性和交互作用后,得出山药多糖浸提的最佳工艺条件:料水比1:17.5(W:V),浸提温度83 ℃,浸提时间3 h.在最佳工艺条件下,山药多糖的实际1次提取率可达2.56%.     

响应面优化野生山毛豆多糖提取工艺的研究

利用响应面分析法对野生山毛豆多糖的提取工艺进行优化.以浸提时间、浸提温度及水料比为响应因子,多糖提取得率为响应值,根据中心组合试验设计,做3因素3水平响应面分析.分析各个因素的显著性和交互作用,得出野生山毛豆多糖水浸提的最佳工艺条件为:浸提时间2.70 h,浸提温度为72 ℃,液料比23.4 mL/g,该条件下多糖提取率为16.83 mg/g.     

响应面分析法优化女贞子水溶性多糖提取工艺

研究女贞子水溶性多糖的提取工艺.在单因素试验基础上选取试验因素与水平,根据中心组合(Box-Benhnken)试验设计原理采用三因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以女贞子水溶性多糖提取率为响应值作响应面和等高线.在分析各个因素的显著性和交互作用后,得出女贞子水溶性多糖浸提的最佳工艺条件为:料水比1:15(g/mL),浸提温度94℃,浸提时间3 h;浸提2次,女贞子水溶性多糖的实际一次提取率可达5.260%.     

杜仲叶多糖提取工艺研究

针对杜仲叶多糖的提取,通过单因素试验选取试验因素与水平,根据Box-Benhnken的中心组合试验设计原理,在单因素试验的基础上采用三因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因素,以多糖提取率为响应值作响应面和等值线图。结果表明,杜仲叶多糖水浸提的最佳工艺条件为：提取温度84℃,浸提时间1.3h,料水比1：14.3。浸提一次,杜仲叶多糖的提取率达到1.7756%。     

Box-Behnken响应面法优化蛇六谷多糖的提取工艺

目的 优化蛇六谷多糖的提取工艺。方法 以多糖得率为评价指标, 以料液比、浸提温度、浸提时间、提取次数为考察对象, 使用单因素实验确定各因素的水平范围, 使用响应面法分析法优化蛇六谷多糖的提取工艺, 最终得出蛇六谷多糖最佳提取工艺。结果 经单因素结合响应面法得出蛇六谷多糖最佳的提取工艺条件为: 料液比1:16 (g/mL), 浸提温度83 ℃、浸提时间1.6 h, 提取2次, 在该条件下, 蛇六谷多糖的提取率为23.56%, 与模型的预测值相比结果相符合。结论 使用单因素结合响应面法优化得出的蛇六谷多糖的提取工艺合理可行, 可用于蛇六谷多糖的提取。     

桑叶多糖提取工艺研究

利用响应面法(Response Surface Methodology)对桑叶多糖的提取工艺进行优化.在单因素实验基础上选取实验因素与水平,根据中心组合(Box-Benhnken)实验设计原理采用三因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以桑叶多糖提取率为响应值作响应面和等高线.在分析各个因素的显著性和交互作用后,得出桑叶多糖浸提的最佳工艺条件为:料水比1 ∶ 19,浸提温度96℃,浸提时间2.5h;浸提2次,桑叶多糖的实际一次提取率可达3.094%.     

响应面分析法优化莲花蜂花粉多糖提取工艺研究

为研究水提法提取莲花蜂花粉多糖的条件,在单因素试验的基础上,根据Box-Behnken 试验设计原理,选取料液比、浸提时间和浸提温度三因素三水平进行响应面分析,建立多糖提取率的二次回归方程,得到提取工艺的优化组合条件。结果表明：料液比、浸提时间和浸提温度对莲花蜂花粉多糖提取率都有显著影响,当提取工艺条件为料液比1:9.4(g/mL)、浸提时间2.4h、浸提温度81.6℃、浸提2 次时,莲花蜂花粉多糖提取率预测值为1.2201%、验证值为1.2317%。     

响应面法优化石花菜琼脂多糖提取工艺

利用响应面分析（RSA）对石花菜琼脂多糖的提取工艺进行优化,选取料液比、提取时间、提取温度和提取次数为试验因素与水平,根据Box-Benhnken中心组合试验设计原理,采用四因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因子,以石花菜琼脂多糖提取率为响应值作响应面和等高线。结果表明：石花菜琼脂多糖浸提的最佳工艺条件为：料液比32∶1mL/g、提取时间1.9h、提取温度100℃、提取次数3次。在最佳工艺条件下,石花菜琼脂多糖的实际1次提取率可达28.85%。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press