Monoclonal antibodies IPO-4 recognizing antigen-activated human T and B lymphocytes

Spleen cells from BALB/c mice previously immunized with B-lymphoblastoid cell line RPMI-1788 were fused with P3-X-63-Ag8.653 myeloma cells. Monoclonal antibodies (Ab) IPO-4 were screened on 18 cell lines by the indirect immuno-fluorescence method. Cryostat sections of tissues were stained according to the PAP technique. The Mab IPO-4 were tested for reactivity with blood cells of 17 healthy persons and 102 patients with chronic lymphocytic leukemia, acute lymphoblastic and myeloblastic leukemias, hairy cell leukemia, multiple myeloma, non-Hodgkin's lymphomas and Hodgkin's disease and mitogen stimulated lymphocytes. MAb IPO-4 were found to be directed against antigen expressed on activated T and B cells.     

New method for direct G/T measurement using satellite signals

A new possible method for direct G/T measurement using satellite signals is described. A formula for calculation of G/T from Y-factor and down link EIRP measurement is developed. The potential of the method for low G/T measurement is illustrated with possible applicability to Intelsat Standard B and C and ECS earth stations. Possible disadvantages are commented on.     

T2-selective magnetization preparation pulses.

The purpose of this work was to present and evaluate a new method for directly designing T2-selective preparation pulses. Using a modified Shinnar-Le-Roux (SLR) transform, the design of T2-selective pulses becomes equivalent to designing a pair of polynomials one of which represents the longitudinal magnetization and the other the transverse magnetization. The polynomials enable one to directly analyze the various tradeoffs involved in the design. To evaluate the new method, a short-T2-selective magnetization preparation pulse was designed. Following the preparation pulse, a 2D Fourier transform (2DFT) multislice gradient echo sequence was used for imaging. For verification Bloch equation simulations were performed along with both in vivo and phantom scans. Phantom scans showed good signal suppression of long-T2 species. This is supported by good long-T2 signal suppression seen on the in vivo images. Simulations indicate that the pulse is robust to +/-150 Hz B0 inhomogeneities and +/-10% B1 inhomogeneities.     

3B2T-RBS?an efficient transmission method for digital fibre-optic communications

A unique transmission method is proposed which utilises the advantages of alphabetic line codes with differential modulation. The method incorporates relative bipulse signalling (RBS) to transmit over optical fibres the symbols of 3B2T ternary alphabetic line code. Together with relative monopulse signalling, the 3B2T code may prove to be an optimum solution for a universal line code in digital communication systems.     

2—3混值电路中的3B—2T编码技术＊

本文通过对2-3混值电路中信号编码效率与冗余度的讨论,分析了传统的1T-2B编码的缺点,提出了3B-2T编码方案,设计了用于3B-2T编码的CMOS接口电路。讨论表明,利用该编码的电路具有效率高,设计容易,并能在不增加电路内部连线的前提下减少三分之一的外部信号线等优点。     

基于ARM的便携式1553B总线测试系统的设计与实现

为了方便对1553B设备进行测试,介绍了一种基于ARM9平台和FPGA的1553B总线测试系统的设计与实现方法。该系统以LPC3250作为微处理器。以CYCLONEI系列的EP1C6Q240C8芯片实现ARM与1553B协议芯片的接口逻辑。在Linux操作系统2．6内核下实现1553B的驱动程序。1553B协议芯片采用BU-64843T8,以实现系统的便携性。     

P-L波段多通道数字收发组件设计

通过P-L波段数字T/R组件的研制,实现了DC-2000MHz频率范围内任意波形信号的射频直接产生和射频直接采样,并通过JESD204B协议和同步时钟驱动的设计,实现了多数字通道间的收发同步,最终通过上位机软件实现对多数字收发通道的波形、频率、相位等参数的实时控制。     

Operational behaviour of a power plant consisting of three generators by B.F. technique

In this paper, a non-repairable parallel redundant complex system similar to a power plant has been studied. The object of the system is to supply power, from a power house having three generators connected in parallel redundancy, to a very critical destination, connected by cables and transformers. The availability and M.T.T.F. have been evaluated by using the Boolean Function (B.F.) technique. The failure times of the various components of the system follow arbitrary distributions. Numerical examples with graphs have also been given to highlight the availability and M.T.T.F. of the system at any time.     

一种S波段高功率T/R组件的研制

介绍了一种S波段高功率T/R组件的研制方法和关键技术。该T/R组件包含4个通道,功能结构复杂,共有4个发射支路和12个接收支路,满足实际应用中实现多路信号的接收。12路接收信号通过3个独立的1∶4功分器合成到3个输出端口,对功分器进行仿真优化电路。发射通道输出功率大于100 W,效率达到40%以上;12路接收通道增益平坦度和增益一致性均在1 d B以内。由于组件使用较多的裸芯片和封装器件,对工艺流程也做了简要介绍。测试结果表明,组件满足各项指标要求。     

Increasing antigenicity of B16 melanoma cell fraction with microwave hyperthermia

An injection of a fraction of a fraction (C30 mw) obtained by centrifugation at 30 kg of homogenate of B16 melanoma cells, treated with microwave hyperthermia (2450 MHz, CW, 44 degrees C, for 20 minutes), increases the survival time (p less than 0.01) of C57-BL/6J male mice inoculated 26 days later with 10(7) cells (viability greater than 95%) of B16 melanoma. This delay of 26 days between these two injections corresponds to the moment where the synthesis of immunoglobulins is increased in protected mice. The earliest death (p less than 0.001) of animals injected with untreated tumoral cell fraction (C30), and that received a suspension of viable B16 melanoma cells (10(7)) can be explained by an inhibition of humoral immunity system.     

超深亚微米无负载四管与六管SRAM SNM的对比研究

采用基于物理的α指数MOSFET模型与低功耗传输域MOSFET模型，推导了新的超深亚微米无负载四管与六管SRAM存储单元静态噪声容限的解析模型．对比分析了由沟道掺杂原子本征涨落引起的相邻MOSFET的阈值电压失配对无负载四管和六管SRAM单元静态噪声容限的影响。     

胎儿磁共振成像特定吸收率及B1 场均匀性研究

为了分析胎儿磁共振成像（MRI）检查中特定吸收率(Specific Absorption Rate, SAR)分布以及B1 场均匀性， 本文分别建立了未怀孕和怀孕女性盆腔电磁模型，参照临床常规，在1.5T 鸟笼发射线圈激励下，采用时域有限差分 (Finite Difference Time Domain, FDTD)法分别对未怀孕和怀孕女性盆腔的SAR 值分布以及B1 场均匀性进行数值计算。 计算结果表明，对B1 场的均匀性而言，前者优于后者；对于相同组织的SAR 值而言，后者均高于前者。特别地，胎 儿各组织的SAR 值普遍较高，有的接近安全阈值。实验结果表明，在胎儿MRI 检查中，应充分考虑可能给胎儿带来 的潜在危害，检查时间不宜过长，检查频率不宜过高。     

JESD204B接口协议中的8B10B编码器设计

JESD204B作为SERDES接口的最新标准协议,相较于传统的接口标准,在众多方面有着明显的优势,可支持高达12.5Gbps的多通道同步和串行数据传输。基于JESD204B接口协议设计和实现了一种新型8B10B编码器。利用极性信息简化编码码表;利用3B4B与5B6B并行编码提升电路工作频率;利用人为加入一位均衡信息,减少逻辑处理层数。同时对协议规定之外的控制字节作以特殊处理。仿真结果表明,该电路完全符合协议规范,并在电路面积、功耗及最大工作频率等方面具有一定优越性。     

A novel Xenopus oocyte expression system based on cytoplasmic coinjection of T7-driven plasmids and purified T7-RNA polymerase.

The Xenopus laevis South African frog oocyte is a well suited and widely used system for protein biochemistry and functional studies. So far, two methods are commonly in use for the expression of exogenous proteins in this system. Investigators have the choice between cytoplasmic injections of in vitro synthesized cRNA or nuclear injections of cDNA. Here, we describe a new method for ion channel expression in oocytes, which consists of a coinjection of T7-driven cDNA and T7-RNA polymerase directly into the cytoplasm. This technique uses very limited amounts of purified enzyme and is also applicable to SP6 polymerase. Commercially available polymerases can also conveniently substitute for self-purified enzymes. The technique can be used for electrophysiological and biochemical analysis. In particular, high level expressions have been achieved for potassium (Shaker B, Kv1.2 and Kv1.3) and sodium (P mu 1.2) channels, and we also demonstrate efficient metabolic labeling of the calcium channel auxiliary beta 3 subunit. The properties of the channels expressed by this technique are indistinguishable from those of the channels expressed by classical methods. Expression of multi-subunit proteins was also achieved illustrating that the technique can be used for structure-function analyses. Moreover, this novel expression technique avoids many drawbacks of the two former techniques. It clearly bypasses the costly and time-consuming step of cRNA synthesis in vitro, prevents delicate cRNA manipulation and is easier to perform and more reliable than nuclear injection. Finally, it does not affect cell survival rate. These data indicate that the T7-RNA polymerase expression technique could be widely used in the future for the expression of exogenous proteins in the Xenopus oocyte system.     

JESD204B接口协议中的8B/10B解码器设计

JESD204B是一种用于数据转换器和逻辑器件内部高速互连的行业新标准,可支持高达12.5 Gbit/s的多通道同步和串行数据传输。设计和实现了一种符合JESD204B协议规范的8B/10B解码器,除了能够正确解码外,还包括控制字符、判断电路、数据极性检测和错误码字检测电路。利用极性信息简化了解码电路,利用组合逻辑提高了检错和极性检测速度,采取并行处理的拓扑结构加快了电路运行速度。跟其他典型电路相比,在芯片面积上缩小了近50%,最高工作频率提高了25%,满足JESD204B协议的指标要求。     

Supporting B2B Business Documents in XMLWeb Services

While XML web services become recognized as a solution to business-to-business transactions, there are many problems that should be solved. For example, it is not easy to manipulate business documents of existing standards such as RosettaNet and UN/EDIFACT EDI, traditionally regarded as an important resource for managing B2B relationships. As a starting point for the complete implementation of B2B web services, this paper deals with how to support B2B business documents in XML web services. In the first phase, basic requirements for driving XML web services by business documents are introduced. As a solution, this paper presents how to express B2B business documents in WSDL, a core standard for XML web services. This kind of approach facilitates the reuse of existing business documents and enhances interoperability between implemented web services. Furthermore, it suggests how to link with other conceptual modeling frameworks such as ebXML/UMM, built on a rich heritage of electronic business experience.     

B2O3掺杂对Ba0.6Sr0.4TiO3/MgO陶瓷介电性能的影响

采用XRD和SEM对B2O3掺杂Ba0.6Sr0.4TiO3/MgO(简称为BSTM)陶瓷致密化行为和介电性能进行了研究。结果表明:掺杂适量B2O3可明显降低BSTM陶瓷的烧结温度,在1480℃时即可烧结致密化,比未掺杂的BSTM陶瓷烧结温度降低70℃;掺杂量不同,则B2O3在陶瓷体中的存在形式不同,引起其介电性能相应变化;1480℃下烧结,B2O3掺杂量为0.8%(质量分数)时,10kHz下测得试样的εr为138,tanδ为0.0034,εr可调率达12.6%(3MV/m),性能有所提高。     

8T vs. 6T SRAM cell radiation robustness: A comparative analysis

We analyze and compare the impact of radiation-induced transient effects based on evaluating the critical charge parameter for 6T and 8T SRAMs during hold, read and write operations. Results on a commercial 65 nm CMOS technology show that 6T and 8T cells offer quite similar robustness when they are in hold. However, the critical charge observed in other operation modes is reduced a 55% respect to the hold operation. For this reason, we provide a thorough analysis of the critical charge behavior in 6T and 8T SRAMs to determine the dependence of memory radiation robustness with memory state. Single event upsets and single event transients have been considered in the analysis, showing that 8T have better performance than 6T. The dependence of critical charge with memory state for high workload memories modifies the overall memory SER estimation indicating the significance of analyzing the memory robustness as a memory state function. In general, the SER estimation results show that the robustness behavior of 8T-based cells is better than robustness behavior of 6T-based cells.     

中国移动B2B电子商务新模式

本文从管理创新的概念出发,以信息化手段实现采购过程中订货流程和ERP订单自动录入为例,介绍中国移动B2B电子商务新模式。文章理论和实践相结合,分析B2B电子商务模式是在采购工作中实现＂低成本高效运营＂目标的一项重要管理创新活动,是对＂集中化、标准化、信息化＂集中管理理论的重要实践。     

Supporting B2B Business Documents in XML Web Services

While XML web services become recognized as a solution to business-to-business transactions, there are many problems that should be solved. For example, it is not easy to manipulate business documents of existing standards such as RosettaNet and UN/EDIFACT EDI, traditionally regarded as an important resource for managing B2B relationships. As a starting point for the complete implementation of B2B web services, this paper deals with how to support B2B business documents in XML web services. In the first phase, basic requirements for driving XML web services by business documents are introduced. As a solution, this paper presents how to express B2B business documents in WSDL, a core standard for XML web services. This kind of approach facilitates the reuse of existing business documents and enhances interoperability between implemented web services. Furthermore, it suggests how to link with other conceptual modeling frameworks such as ebXML/UMM, built on a rich heritage of electronic business experience.