Vineyard variability in Marlborough,New Zealand: characterising spatial and temporal changes in fruit composition and juice quality in the vineyard

Background and Aims: Previous work has demonstrated that vineyards are spatially variable and that this variability can be understood in terms of the underlying characteristics of the land (soils, topography) supporting the vineyard. Selectively harvesting blocks in response to such variability may be highly profitable. While it has also been shown that crop maturation is spatially variable, there may also be temporal variations in the rate of maturation. Integrating knowledge of how spatial variation in fruit composition may be moderated in time has not previously been attempted and is the key objective in this work. Methods and Results: We used a proximal sensor to map vine vigour at high spatial resolution in a 5.9‐ha Marlborough vineyard planted with Sauvignon Blanc. Vigour measurements were also related to fruit‐soluble solids (SS), titratable acidity (TA) and pH – key indices of crop maturity. Knowledge of crop phenology and maturation was used to predict how these indices changed with time. The pooled opinions of over 50 Marlborough winemakers on the optimum juice SS, pH and TA at harvest to produce a ‘typical Marlborough Sauvignon Blanc’ were used to develop a juice index (JI), which in turn was mapped in space and time at the study site. The JI showed marked spatial and temporal variation. Conclusions: In addition to being spatially variable, grape quality in vineyards also changed with time. Thus, the optimisation of decisions about harvest timing requires knowledge of spatial variability. Conversely, strategies such as selective harvesting cannot be properly optimized without knowledge of crop phenology, the maturation of fruit and their implications for fruit quality – which are all also spatially variable. In this study, we have shown that, by integrating knowledge of crop phenology with an understanding of vineyard variability and winemaker objectives through the construction of a JI, it is possible for the optimum harvest decision to be made such that fruit destined for a particular end use are harvested at the right time and from the right place. Significance of the Study: This is the first study in which knowledge of both spatial and temporal vineyard variation has been integrated. It demonstrates that in order to be optimal, strategies such as selective harvesting need to incorporate knowledge of crop phenology rather than rely on knowledge of spatial variation alone.     

Effect of storage temperature on the chemical composition and sensory profile of Sauvignon Blanc wines

Background and Aims: Most white wines lose fresh and fruity characteristics, associated with volatile esters, during ageing in the bottle. A higher storage temperature accelerates these changes. The aim of this study was to examine the influence of storage temperature on the chemical and sensory properties of Sauvignon Blanc wines. Methods and Results: Three commercially bottled Sauvignon Blanc wines from the 2008 and 2009 vintages were stored at 5, 10, 18°C and at room temperature for 12 months. Wines stored at warmer temperatures (18°C and room temperature) contained lower concentrations of acetate esters, including the prominent varietal thiol 3MHA, and ethyl esters of fatty acids, than the wines stored at cooler temperatures (5 and 10°C). A warmer temperature accelerated the rate of ester hydrolysis. Conversely, the concentrations of ethyl esters of branched acids were higher in wines stored at the warmer temperatures. The sensory profile of the wines was assessed after 12 months for the two 2008 wines and after 8 months for the 2009 wine. The wines stored at cooler temperatures were characterised by higher fruity and fresh vegetal aromas, whereas the wines stored at warmer temperatures exhibited the opposite sensory profile, with dominant woody/smoky/oaky, buttery, flinty and canned asparagus notes. Conclusions: These results indicate that temperature‐dependent hydrolysis processes are critical for Sauvignon Blanc aroma stability during the first year in the bottle. Significance of the Study: Cool storage temperature conditions can significantly increase the shelf‐life of Sauvignon Blanc wines by preserving their fruity and fresh green characters.     

The effect of mechanical harvesting and transport of grapes, and juice oxidation, on the protein stability of wines

The concentration of haze-forming protein was determined in wines made from Sauvignon Blanc grapes harvested by hand or machine and transported for up to 400 km. The effect of oxidation of juice from mechanically harvested fruit on the concentration of haze-forming proteins was also investigated. Wines made from mechanically harvested, long-distance transported fruit contained more heat-unstable protein, and required fining with twice as much bentonite to ensure heat stability as wines made from hand-picked fruit. Juice oxidation had little effect on protein content of the juice or of the wine made from it.     

Co-fermentation with Pichia kluyveri increases varietal thiol concentrations in Sauvignon Blanc

Background and Aims:  Volatile varietal thiols are one class of yeast metabolite that add aroma/flavour to Sauvignon Blanc, particularly to the Marlborough style from New Zealand. While various strains of Saccharomyces cerevisiae are known to liberate such thiols, there are no data examining whether non- Saccharomyces species effect concentrations of these thiols in wine.
Methods and Results:  We screened a variety of New Zealand wine yeast isolates and found some that were able to liberate either 3-mercaptohexan-1-ol (3MH) or 3-mercaptohexyl acetate (3MHA). We show that co-fermentation with specific commercial strains and an isolate of Pichia kluyveri from New Zealand, at a 1:9 starting ratio, significantly enhances the 3MHA concentrations in Sauvignon Blanc compared with single species ferments.
Conclusions:  Co-fermentation with this isolate of P. kluyveri may provide a means to elevate 3MHA concentrations in Sauvignon Blanc; the mechanism behind this increase is unknown.
Significance of the Study:  Techniques that allow the predictable manipulation of varietal thiols potentially provide tools for winemakers to alter wine style without the use of genetically modified organisms.     

Evaluating the value of replicate tastings of a given wine: bio-statistical considerations

Wine judges were randomly selected from the membership of the American Association of Wine Economists (AAWE) to participate in a blind triplicate tasting of two South African varietals: one Sauvignon Blanc and one Pinotage; as well as a blind tasting of a single pouring of five additional Sauvignon Blanc wines and five additional Pinotage wines. It was hypothesized that those wine Tasters who scored the triplicate wines reliably would also score the single poured wines reliably; and, conversely, those who were unreliable on the triplicate wines would also be unreliable on the additional five wines. The results were substantially confirmative for the Sauvignon varietals, but only minimally confirmative for the Pinotage varietals. Two possible reasons are, first, the simpler structure of Sauvignon Blanc (a white wine) and possible palate fatigue for the more complex Pinotage (a red wine).     

Can spectroscopy geographically classify Sauvignon Blanc wines from Australia and New Zealand?

The combination of UV, visible (Vis), near-infrared (NIR) and mid-infrared (MIR) spectroscopy with multivariate data analysis was explored as a tool to classify commercial Sauvignon Blanc (Vitis vinifera L., var. Sauvignon Blanc) wines from Australia and New Zealand. Wines (n = 64) were analysed in transmission using UV, Vis, NIR and MIR regions of the electromagnetic spectrum. Principal component analysis (PCA), soft independent modelling of class analogy (SIMCA) and partial least squares discriminant analysis (PLS-DA) were used to classify Sauvignon Blanc wines according to their geographical origin using full cross validation (leave-one-out) as a validation method. Overall PLS-DA models correctly classified 86% of the wines from New Zealand and 73%, 86% and 93% of the Australian wines using NIR, MIR and the concatenation of NIR and MIR, respectively. Misclassified Australian wines were those sourced from the Adelaide Hills of South Australia. These results demonstrate the potential of combining spectroscopy with chemometrics data analysis techniques as a rapid method to classify Sauvignon Blanc wines according to their geographical origin.     

The influence of yeast on the aroma of Sauvignon Blanc wine

The main objective of this study was to investigate the effect of different Saccharomyces cerevisiae wine yeast strains on the concentration of aroma-enhancing volatile thiols and fermentation metabolites in Sauvignon Blanc wine. Seven commercial wine yeast strains were selected based on their putative ability to modulate the concentrations of the fruity volatile thiols, 4-mercapto-4-methylpentan-2-one (4MMP), 3-mercapto-hexanol (3MH) and 3-mercapto-hexylacetate (3MHA). Each of these yeasts was used to produce Sauvignon Blanc wines under controlled conditions, in triplicate, in 20-L quantities. The levels of 4MMP, 3MH and 3MHA in these wines were quantified using the p-hydroxymercuribenzoate method. In addition, a total of 24 volatile yeast-derived fermentation aroma compounds were also quantified using headspace solid-phase microextraction coupled with gas chromatography mass spectrometry (HS-SPME–GC–MS). Formal sensory analysis was conducted by 12 trained assessors and, additionally, a panel of 24 experienced wine industry professionals assessed the wines and ranked them in order of preference. The results indicated that the yeast strains varied significantly in terms of their capabilities to (i) produce volatile thiols and fermentation metabolites; and (ii) to modulate the varietal characters of Sauvignon Blanc wine. Yeast strains that produced the highest levels of volatile thiols were responsible for wines with the highest perceived intensity of fruitiness, and these wines were preferred by the tasting panels. While the ‘green’ characters in Sauvignon Blanc wines can be manipulated through vineyard management, the ‘tropical fruity’ characters appear to be largely dependent on the wine yeast strain used during fermentation. Therefore, the choice of yeast strain offers great potential to modulate wine aroma profiles to definable styles and predetermined consumer market specifications.     

Variation in vine vigour,grape yield and vineyard soils and topography as indicators of variation in the chemical composition of grapes,wine and wine sensory attributes

Background and Aims: Vineyard variability makes satisfaction of winemaker demands for uniform parcels of fruit that are suitable for particular product streams difficult. Indeed, it may not be possible to satisfy these demands without being able to adequately characterise differences between wines derived from different fruit parcels or different areas of the same vineyard, understanding how final wines are affected by management decisions implemented in the vineyard, and/or understanding the effects of variation in the vineyard's biophysical characteristics (e.g. soil, topography) on grape and wine composition. This work sought to identify and examine relationships between the chemical and sensory attributes of wines derived from different parts of the same block and the biophysical characteristics of these different vineyard areas. Methods and Results: Remote sensing of vine vigour, yield mapping and EM38 soil survey were used to identify zones of contrasting vineyard performance in a Cabernet Sauvignon vineyard in the Murray Valley region. Small‐lot wines were made from fruit sourced from these zones. Both sensory and chemical analysis (solid phase microextraction‐gas chromatography‐mass spectrometry) of these wines demonstrated them to be different. Likewise, soil properties and indices of vine nutrition differed between the zones. Conclusions: This work suggests that it is possible for robust relationships to be established between specific (manageable) biophysical attributes of the place where grapes are grown and the sensory and chemical characteristics of the wines derived from them. It also supports the view that terroir is spatially variable at the within‐vineyard scale. Significance of the Study: The work provides a foundation for further research aimed at establishing how specific sensory and/or chemical properties in wines might be modified through targeted management interventions in the vineyard.     

Effect of Esca disease on the phenolic and sensory attributes of Cabernet Sauvignon grapes,musts and wines

Background and Aims: The impact of Esca, a cryptogamic disease affecting woody tissues of grapevines (trunk, branches and shoots), was investigated on phenolic and sensory qualities of Cabernet Sauvignon grapes, derived musts and wines. Methods and Results: Global phenolic analyses revealed no major difference between healthy and affected grapes whereas high‐performance liquid chromatography analyses showed decreases in the skin concentrations of catechin and epicatechin and of anthocyanins for the two vintages assessed. Proanthocyanidin characteristics, and particularly mean degree of polymerisation, were strongly decreased in skin proanthocyanidin polymeric fractions. Chemical analyses of wines made with different percentages of fruit from Esca infected grapevines confirmed the moderate impact of Esca on phenolic composition. Nevertheless, sensory analyses revealed a loss of wine sensory quality perceptible with as little as 5% of affected fruit in wines. Conclusions: Phenolic variations in grapes and in derived wines may be related to changes to grapevine physiology caused by Esca fungi that impact on flavonoid metabolism. The chemical and sensory variations between wines can also be related to the delayed ripening delay of fruit from Esca infected grapevines. Significance of the Study: Esca moderately affected the phenolic composition of grapes and decreased the sensory quality of wines, suggesting a dramatic increase in the economic importance of Esca if no control methods are found.     

Environmental effects on cv Cabernet Sauvignon (Vitis vinifera L.) grown in Hawke's Bay, New Zealand.: 1. Phenology and characterisation of viticultural environments

Six Cabernet Sauvignon vineyard sites in Hawke's Bay (New Zealand), selected out of 28 sites observed in 1996/97, were studied over three consecutive seasons in order to characterise viticultural environments of this region for this cultivar. Indices of precocity of vine phenology were used to analyse the relationship between phenology at the selected sites and vegetative growth, productivity, fruit ripening and wine sensory characteristics. Marked differences in indices of precocity existed between sites. These differences were mostly correlated with vine vegetative growth and canopy indices. There was no relationship between yield and vine phenology. Indices of precocity were correlated with certain juice constituents measured on a common date, as well as the sensory scores of wines produced by microvinification from grapes harvested on different dates and maturity levels. Vineyard sites that differed in indices of precocity also differed in their environmental characteristics, particularly in soil physical properties and water balance.     

Astringency sub-qualities of red wines and the influence of wine–saliva aggregates

Astringency is a sensory attribute, related to the quality and mouthfeel of red wines. However, the origin of astringency sub-qualities, such as the typical drying astringency found in immature grapes, is still unknown. Astringency of red wines with similar tannin content but different astringency sub-qualities, from different harvest dates, is studied. Astringency was characterised in terms of friction coefficient, polyphenol content, sensory analysis and tannin/salivary–proteins aggregates characterisation. A different evolution during ripening was found for both Cabernet Sauvignon and Carménère, and tannin–protein aggregates showed differences in size, shape and surface. The velvety sub-quality appears to be related to aggregates with low precipitation, and with specific surface characteristics as roundness and Feret diameter. Results from this work propose an effect of aggregates on sensory perception and opens the possibility to explore their effect on oral lubrication.