Rootstock effects on salt tolerance of irrigated field-grown grapevines (Vitis vinifera L. cv. Sultana) 2. Ion concentrations in leaves and juice

Field vines of cv. Sultana, grown either on their own roots, or grafted to a range of rootstocks, were drip‐irrigated at three salinity levels (0.40, 1.75 and 3.50 dS/m) over a five‐year period. Rootstocks comprised Ramsey, 1103 Paulsen, J17‐69 and 4 hybrids (designated R1, R2, R3 and R4) derived from parentage involving Vitis champini, V. berlandieri and V. vinifera. Concentrations of Cl^‐, Na+, K+, Ca²⁺ and Mg²⁺ were measured in petioles at flowering, and in laminae and grape juice at harvest, in each year of the trial. Vines on all rootstocks accumulated less chloride in either petioles at flowering or in laminae and juice at harvest compared with vines on own roots at all salinity treatments. By inference, all rootstocks behaved as chloride excluders relative to the roots of own‐rooted vines. 1103 Paulsen was the best chloride excluder based on lowest concentrations of accumulated Cl^‐ in petioles, laminae and grape juice at high salinity. Sultana on R3 rootstock at high salinity accumulated more Na+ in both laminae and grape juice (at harvest) than did Sultana on own roots or on any of the other rootstocks. Laminae K+ at harvest time was reduced at high salinity in Sultana on own roots and on all rootstocks. Concentrations of both Cl^‐ and Na+ in petioles at flowering and in laminae and grape juice at harvest showed no significant correlation with either yield (as kg of fresh grapes per vine) or vigour (as measured by fresh weight of one‐year‐old pruning wood per vine) for any salinity treatment. There was however, a strong positive correlation between yield and the subsequent weight of one‐year‐old pruning wood for all salinity treatments. There was also a negative correlation between Na+ concentrations in petioles at flowering and the subsequent weight of one year‐old‐pruning wood from the 0.40 dS/m treatment. Similar negative correlations were found between Na+ concentration in both laminae and grape juice at harvest time, and the subsequent weight of one‐year‐old pruning wood from the 0.40 dS/m treatment (but not from either the 1.75 or 3.50 dS/m treatments). Based on these findings and those from Walker et al. 2002a we conclude that a high innate vigour of a rootstock combined with moderate to high chloride and sodium exclusion ability represents the best combination for salt tolerance in Sultana grapevines as measured by yield at moderate to high salinity.     

A diminished capacity for chloride exclusion by grapevine rootstocks following long-term saline irrigation in an inland versus a coastal region of Australia

Shiraz and Chardonnay grapevines that had been initially assessed for Cl^‐ exclusion in the 1996 and 1997 harvest seasons at both Merbein (inland region of Victoria) and at Padthaway (sub‐coastal region of South Australia), were re‐evaluated in 2003 and 2004 at those same two locations. Both scion varieties were grown either on their own roots or were grafted to one of the following rootstocks: Ramsey, 1103 Paulsen, 140 Ruggeri, K51‐40, Schwarzmann, 101‐14, Rupestris St George and 1202 Couderc. Both sites had been irrigated with saline water since 1994. The salinity of irrigation water at Merbein was approximately 2.1 dS/m; at Padthaway irrigation salinity varied between approximately 1.6 dS/m and 2.5 dS/m during the survey period. Changes in the Cl^‐‐excluding ability of all rootstock/scion combinations between 1996, 1997 and 2003, 2004 were based upon analysis of Cl^‐ concentrations in grape juice and in laminae at harvest, as well as Electrical Conductivity (EC), pH and Sodium Adsorption Ratio (SAR) of the 1:5 soil:water extract. Taking juice Cl^‐ as indicative of rootstock effectiveness for Cl^‐ exclusion, and considering data from Merbein, our analyses demonstrated that overall means for Shiraz juice Cl^‐ increased from around 190 to 427 mg/L over the survey period (7 years). In Chardonnay grapevines at Merbein, overall mean concentrations of Cl^‐ in juice increased from around 70 to around 225 mg/L over 7 years. Significant differences between rootstocks were evident, with some rootstocks at Merbein showing a diminished capacity for Cl^‐ exclusion in 2003 and 2004. By contrast, in Padthaway there was no consistent deterioration in Cl^‐‐excluding capacity by rootstocks supporting either Shiraz or Chardonnay as scions. In 2004 there were significant differences between grapevines at Merbein and Padthaway in the concentration of Cl^‐ accumulated, even though the mean soil EC_1:5 after harvest for 0–90 cm depth was the same at both sites, namely 0.4 dS/m. Containment of grapevine salinity at Padthaway (relative to Merbein) between 1996/97 and 2003/04 was most likely due to factors such as a lower volume of saline irrigation, double the rainfall and 27% lower pan evaporation.     

Effect of reduced irrigation on growth,yield, ripening rates and water relations of Chardonnay vines grafted to five rootstocks

Background and Aims: In the first decade of the 21st century, drought within the Murray–Darling Basin has reduced the amount of water available for irrigation. We investigated whether the response of vines to reduced irrigation was modified by rootstock. Methods and Results: Reduced irrigation (5 versus 8 ML/(ha·year)) was applied to Chardonnay vines grafted to five rootstocks (Ramsey, 140 Ruggeri, 1103 Paulsen, 110 Richter and K51‐40) for four seasons. It decreased the yield from 29.3 to 26.7 kg/vine, and increased the irrigation water use index (IWUI) from 4.7 to 6.6 t/(ha·ML), but gains in this index declined as the trial progressed. The values of mid‐afternoon leaf water potential were not affected by reduced irrigation, but leaf CO₂ assimilation declined from 13.1 to 11.7 µmol/(m²·s). These effects were independent of rootstock. Reduced irrigation did not increase soil salinity (ECe) or vine tissue Na and Cl concentrations. Vines on Ramsey and 1103 Paulsen rootstocks had higher yields, 32.2 and 30.0 kg/vine, respectively, and the highest IWUIs, 5.9 and 5.5 t/(ha·ML). In two of the three seasons, reducing irrigation did not affect the rates of ripening (°Brix/growing degree days) excepting vines on 1103 Paulsen. Ripening rates varied by 1.5‐fold between seasons. Conclusion: The yield and growth responses of Chardonnay vines to a 35% reduction in irrigation were not modified by rootstock. Significance of the Study: Reducing irrigation did not lead to a build‐up of soil salts. The response of vines to reduced irrigation on rootstocks rated as having good drought tolerance was the same as that for vines on a rootstock rated as having poor drought tolerance.     

Reduced irrigation and rootstock effects on vegetative growth,yield and its components,and leaf physiological responses of Shiraz

Background and Aims: The study investigated whether rootstocks can modify grapevine responses to reduced irrigation. Methods and Results: Drip-irrigated Shiraz vines on eight rootstocks were subjected to industry standard and 30% reduced irrigation regimes over four seasons. Reducing irrigation decreased pruning weights and yield, but did not consistently affect irrigation water use index (IWUI). It increased leaf Δ¹³C. Reduced irrigation and elevated vapour pressure deficit (VPD) were associated with decreases in leaf water potential (ψ_l), leaf stomatal conductance and assimilation rate. Reducing irrigation raised leaf transpiration efficiency, whereas elevated VPD lowered it. These effects of reduced irrigation were independent of rootstock. Vines grafted to 101-14 had a higher ψ_l and achieved the highest yield and IWUI. The yields of vines grafted to Ramsey, Schwarzmann and 140 Ruggeri were also high. Vines grafted to 101-14, Ramsey and 1103 Paulsen had the higher rates of leaf assimilation. Rootstock did not affect Δ¹³C. Conclusion: The gain in leaf transpiration efficiency caused by reducing irrigation was not associated with a gain in IWUI. Rootstocks 101-14, Ramsey, Schwarzmann and 140 Ruggeri achieved higher yields and IWUI under both standard and reduced irrigation regimes. Significance of the Study: Among grafted vines growing on saline soil but receiving non-saline irrigation water and subject to a 30% reduction in irrigation, the yield responses of vines grafted on rootstocks rated as having good drought tolerance were the same as those of vines grafted on rootstocks rated as having poor drought tolerance.     

Shiraz berry size in relation to seed number and implications for juice and wine composition

This study was conducted over three seasons on irrigated Shiraz grapevines growing in a warm climate. We addressed the question of whether differences in berry size (within a population of berries from minimally pruned, own‐rooted or Ramsey‐grafted vines), would lead to differences in juice composition, wine composition or wine sensory score. Predictably, berry mass was found to increase with seed number, but berries in the smallest mass categories (0.3–0.7 g) still had similar juice soluble solids and pH; and similar concentrations of K+, tartaric acid and malic acid, compared with larger berries (1.4–2.0 g). Only for the very smallest mass category (0.3–0.55 g) was there any indication of better colour density (both for own‐rooted and Ramsey‐grafted vines) or higher anthocyanin concentration (for own‐rooted vines) compared with larger berries (1.4–2.0 g). Concentrations of tartaric acid and K+ in berry skins were highest in the smallest berry mass categories (0.3–0.7 g) and decreased with increasing berry mass (up to 1.4–2.0 g). A strong correlation (R²= 0.85) between skin tartaric acid and K+ concentrations was observed across that range. Small‐scale wine lots based on small berries (0.8–0.9 g) versus large berries (1.2–1.3 g) showed no differences in measures such as soluble solids, total acids or pH of juice; nor any differences in pH, total acids, K+, tartrate, malate, spectral characteristics or sensory score of corresponding wines. Moreover, small berries had a similar skin to fruit ratio, and a similar juice yield, compared to large berries. However, when measured post‐fermentation, the ratio of seed weight to skin weight was higher for small berries. The mass range of berries used here for small‐scale winemaking (i.e. from 0.8–0.9 g up to 1.2–1.3 g), covered the range of Shiraz berry mass typically found in irrigated vineyards (from 0.8 to 1.5 g), and thus confirms the relevance of present outcomes to practical winemaking. Finally, our data for variation in juice and wine composition as a function of berry size, showed consistent trends for all seasons, and thus implies that reported instances of improved wine quality from small berries (often associated with certain pruning treatments or deficit irrigation strategies), are more likely due to treatment effects that lead to small fruit, rather than to intrinsic developmental differences between large and small berries.     

Rootstock and salinity effects on rates of berry maturation, ion accumulation and colour development in Shiraz grapes

Shiraz grapevines on either their own roots, or on the rootstocks Ramsey, 1103 Paulsen, 140 Ruggeri or 101–14, were grown at two separate sites within the Murray‐Darling viticultural region with similar irrigation regimes but with an irrigation water salinity of either 0.43 dS/m (low salinity site) or 2.3 dS/m (high salinity site). Rootstock effects on grape berry development, ion concentrations, soluble solids and acidity were followed during one season. Wines were also made and compared using spectral analysis and sensory evaluation. Rootstock effects that were common across both sites were (1) a close relationship between K+and soluble solids accumulation in developing grape berries which commenced at the onset of veraison and was indicative of a link between K+and sucrose transport in the phloem, and (2), higher wine K+, pH and colour hue for all rootstocks with one exception, namely 101–14 at high salinity where 101–14 responded similarly to own roots. Juice K+, pH and loss of K+from juice during winemaking were highest for grapes from the high salinity site. Mean berry weight was smaller and the range in berry size across rootstocks was narrower at the saline site. The narrower range in berry sizes may have contributed to fewer rootstock effects on wine spectral characteristics at high salinity. There was no effect of rootstock on CO₂ assimilation rate or stomatal conductance at either site, although intrinsic leaf‐based water‐use efficiency measured as A/g was 50% higher at the saline site. All treatments exhibited berry shrivel at maturity, but the extent was smaller at high salinity. Slower development of berry colour during veraison was observed on some rootstocks, for example 101–14, and while unrelated to canopy size per se, a higher leaf‐to‐fruit ratio for 101–14 may have been a factor. Slower berry colour development during veraison had no bearing on the colour density of wine made from the harvested grapes.     

Effect of salinity and Ramsey rootstock on ion concentrations and carbon dioxide assimilation in leaves of drip-irrigated, field-grown grapevines (Vitis vinifera L. cv. Sultana)

Salinity treatments of 0.43, 1.7 and 3.4 dS/m were applied through a drip-irrigation system to four-year-old vines of own-rooted Sultana (S_O) and Sultana on Ramsey rootstock (Sr) The vines were planted in spring 1987 and established for two years under irrigation with low salinity (0.43 dS/m) water before commencing the range of salinity treatments in spring 1989. The effects of salinity and rootstock on yield, size and composition of berries, canopy size, lamina CO₂ assimilation, leaf water relations and lamina ion concentrations were studied between veraison and harvest during the second season of salinity treatments. Mature leaves of S_R had higher rates of CO₂ assimilation (leaf area basis) and stomatal conductance than comparable leaves of S_O. The high salinity treatment (3.4 dS/m) reduced CO₂ assimilation rate of S_O but not of S_R. Medium salinity (1.7 dS/m) had no significant effect on CO₂ assimilation rate of either type. Laminae of S_O accumulated significantly higher concentrations of chloride than S_R vines at all salinity levels. There was a significant negative correlation (r²= 0.44) between CO₂ assimilation rate and laminae chloride of S_O. Leaf sodium concentrations increased with increasing salinity, but concentrations in laminae at high salinity were similar in S_O and S_R, with S_R showing no reduction in CO₂ assimilation. Leaf potassium concentrations were higher in S_R, but decreased with increasing salinity, whereas magnesium concentrations were similar in S_O and S_R vines but increased in both at high salinity. Leaf water potential and relative water content were not significantly affected by salinity in either S_O or S_R vines. Both vine types had reduced total leaf area and pruning wood weights as salinity increased, but they were greater in S_R at all salinity levels because of the higher inherent capacity for biomass production in S_R vines. The bigger canopies, lower lamina chloride concentrations and ‘normal’ photosynthesis rates of S_R vines at 3.4 dS/m enabled these vines to mature crops with similar berry weights, sugar contents and fruit yield compared with those of S_O vines at 0.43 dS/m. The research demonstrated the benefit of using Ramsey rootstock for Sultana under saline field conditions and provided a physiological explanation for their higher salt tolerance.     

Effects of timing and rate of N supply on leaf nitrogen status, grape yield and juice composition from Shiraz grapevines grafted to one of three different rootstocks

Yeast cells have a minimum N requirement to ferment a must through to dryness, so that grape N content (hence must N) becomes critical in meeting that prerequisite. Viticultural practices aimed at meeting that N requirement are of special relevance because interactions between rootstock and vineyard nitrogen supply strongly influence scion mineral nutrient status as well as shoot vigour, and via those processes, fruit composition. Such outcomes were investigated in a field trial involving Shiraz on three rootstocks viz. Teleki 5C, Schwarzmann and Ramsey. Five N supply regimes, varying from 0 to 80 kg/(haseason), were imposed through a drip-irrigation system during two periods (either flowering to veraison, or post-harvest to leaf-fall, or both) over three successive growing seasons. Post-harvest N supply increased scion leaf N and nitrate N concentrations at flowering for vines on Teleki 5C and Schwarzmann. By veraison, N recently applied in the flowering to veraison period elevated these indicators of N status in all vines on all rootstocks. Grape yields from vines on Teleki 5C and Schwarzmann were elevated by N supply after harvest, whereas juice soluble solids levels were lowered. Free amino acids in Shiraz juice were dominated by non-assimilable N, amounting to about 50% or more of the total free amino-N in the juice. Increasing N supply increased free amino acid concentrations in the juice of berries from vines on all rootstocks, but only vines on Schwarzmann derived any benefit from N supplied after harvest. The highest concentrations of free amino acids were measured in the berries from vines on Schwarzmann receiving 80 kg N/(ha.season). Of immediate practical relevance for N management of Shiraz grapevines on either Teleki 5C or Ramsey rootstocks, the minimum value for assimilable free amino-N concentration required to ferment a must through to dryness was not achieved if vineyard N application was limited to the post-harvest period.     

Salt-induced modulation in inorganic nutrients, antioxidant enzymes, proline content and seed oil composition in safflower (Carthamus tinctorius L.)

BACKGROUND: Safflower (Carthamus tinctorius L.) has gained considerable ground as a potential oil‐seed crop. However, its yield and oil production are adversely affected under saline conditions. The present study was conducted to appraise the influence of salt (NaCl) stress on yield, accumulation of different inorganic elements, free proline and activities of some key antioxidant enzymes in plant tissues as well as seed oil components in safflower. Two safflower accessions differing in salt tolerance (Safflower‐33 (salt sensitive) and Safflower‐39 (salt tolerant)) were grown under saline (150 mmol L^?1) conditions and salt‐induced changes in the earlier‐mentioned physiological attributes were determined. RESULTS: Salt stress enhanced leaf and root Na⁺, Cl^? and proline accumulation and activities of leaf superoxide dismutase, catalase and peroxidase, while it decreased K⁺, Ca²⁺ and K⁺/Ca²⁺ and Ca²⁺/Na⁺ ratios and seed yield, 100‐seed weight, number of seeds, as well as capitula, seed oil contents and oil palmitic acid. No significant effect of salt stress was observed on seed oil α‐tocopherols, stearic acid, oleic acid or linoleic acid contents. Of the two safflower lines, salt‐sensitive Safflower‐33 was higher in leaf and root Na⁺ and Cl^?, while Safflower‐39 was higher in leaf and root K⁺, K⁺/Ca²⁺ and Ca²⁺/Na⁺ and seed yield, 100‐seed weight, catalase activity, seed oil contents, seed oil α‐tocopherol and palmitic acid. Other attributes remained almost unaffected in both accessions. CONCLUSION: Overall, high salt tolerance of Safflower‐39 could be attributed to Na⁺ and Cl^? exclusion, high accumulation of K⁺ and free proline, enhanced CAT activity, seed oil α‐tocopherols and palmitic acid contents. Copyright © 2011 Society of Chemical Industry     

Potassium concentration and pH inter‐relationships in grape juice and wine of Chardonnay and Shiraz from a range of rootstocks in different environments

Background and Aims: pH adjustment during winemaking is a significant cost to the Australian wine industry. This study addresses potassium (K⁺) concentration and pH inter‐relationships in grape juice and wine of Chardonnay and Shiraz. Methods and Results: Chardonnay and Shiraz on own roots, and on Ramsey, 1103 Paulsen, 140 Ruggeri, K51‐40, Schwarzmann, 101‐14, Rupestris St. George and 1202 Couderc were compared at Koorlong and Merbein (Victoria), and Padthaway, Nuriootpa and Rowland Flat (South Australia). Petiole K⁺ concentrations at flowering were a poor indicator of grape juice and wine K⁺ concentrations. The concentration of H⁺ ions in grape juice and wine decreased as K⁺ concentrations increased resulting in increased pH. The relationship between H⁺ and K⁺ concentrations was linear for Chardonnay but exponential for Shiraz, where K⁺ concentrations were higher. Wine K⁺ and grape juice K⁺ concentrations exhibited a positive linear relationship, with slope for Chardonnay about half that for Shiraz, indicating a net loss of K⁺ between grape juice and wine of 58% for Chardonnay and 13% for Shiraz. Conclusions: The study has linked higher wine pH to both higher juice soluble solids and K⁺, and to poorer wine colour hue. Loss of K⁺ during fermentation and cold stabilisation appeared higher for Chardonnay than for Shiraz. Significance of the Study: Rootstocks that lead to lower K⁺ concentrations and pH in grape juice and wine are identified. Differences in the K⁺ concentration dynamics between grape juice and wine of Chardonnay and Shiraz are described and quantified.     

Impact of rootstock on yield and ion concentrations in petioles,juice and wine of Shiraz and Chardonnay in different viticultural environments with different irrigation water salinity

Background and Aims: Within-site comparisons were made of rootstock effects on yield, and chloride and sodium concentrations in petioles, juice and wine of Shiraz and Chardonnay vines at sites with irrigation water salinities (EC_iw) ranging from low (0.4 dS/m) to moderate-high (1.8 to 3.3 dS/m). It also compared consistency of yield performance of the various rootstocks with both scions over 8 years at one site with an EC_iw of 2.1 dS/m. Methods and Results: Chardonnay and Shiraz on own roots and on Ramsey, 1103 Paulsen, 140 Ruggeri, K51-40, Schwarzmann, 101-14, Rupestris St. George and 1202 Couderc were compared. Ramsey resulted in better yields relative to most of the other rootstocks at three of the four sites for each scion. Exceptions were the low salinity site where Schwarzmann was best with Chardonnay, and Padthaway where 140 Ruggeri was best with Shiraz. Chardonnay wine chloride concentrations were similar to grape juice chloride concentrations, but Shiraz wine chloride concentrations were on average 1.7-fold higher than grape juice chloride. Conclusions: Shiraz on own roots, K51-40 and 1202C rootstocks carry some risk of accumulating unacceptable levels of chloride in grape juice and wine when the salinity of the irrigation water is at moderate to high levels. Rootstocks K51-40 (with Chardonnay and Shiraz) and potentially 101-14 (with Shiraz) should be avoided in situations of long term irrigation with moderate to high salinity water. Significance of the Study: The study identifies rootstocks with acceptable yields and grape juice chloride concentrations for potential use in regions affected by salinity.     

Management practices for Sunmuscat (Vitis vinifera L.): a new drying variety

Sunmuscat grapevines, managed on one of three trellis × two row spacing configurations, and grown on either their own roots or grafted onto Ramsey rootstock, were assessed over nine seasons (1990–1998 inclusive). The three trellis systems comprised (1) a 0.3 m narrow T‐trellis (NT), (2) a 1.2 m wide T‐trellis (WT), both with a 3.3m row spacing and a height of 1.2 m, and (3) a high (1.8 m) hanging cane (HC) system based on bi‐lateral cordons with a 2.2 m row spacing. Vine spacing within rows was 2.4 m irrespective of row width. A trellis drying treatment was compared with hand harvest during 1991–1995. Over the nine seasons, individual WT vines sustained highest yields of fresh grapes (23.7 kg/vine.season) compared to either NT (20.9 kg/vine) or HC vines (20.8 kg/vine) respectively (P < 0.001). However, HC vines expressed a considerable production advantage on a per hectare basis, viz. 41.3 t/ha, due to closer row spacing. Corresponding vineyard productivity for NT and WT vines was 26.4 and 29.9 t/ha respectively. Both WT and HC vines showed significantly higher bunch and shoot numbers than the NT treatment (P < 0.05 and P < 0.001, respectively). Nevertheless, trellis treatment had no effect on fruitfulness, berry weight, bunch weight, total soluble solids or titratable acidity. Taken overall, vines grafted to Ramsey rootstock had higher mean yields than vines on their own roots (i.e. 23.6 and 20.1 kg /vine, respectively P < 0.001) due to more berries per bunch and larger berries, despite having significantly fewer shoots and bunches. There was no long term evidence for incompatibility of Sunmuscat grafted to Ramsey rootstock. Compared to hand harvest of fresh grapes, trellis drying had no effect on yield, shoot and bunch numbers. Development of significantly smaller berries with trellis drying was offset by higher levels of total soluble solids. Sunmuscat is clearly a highly productive variety and is suited to canopy management on tall, cordon‐based hanging cane systems which are also amenable to trellis drying.     

Rootstock effects on salt tolerance of irrigated field-grown grapevines (Vitis vinifera L. cv. Sultana). 3. Fresh fruit composition and dried grape quality

Field‐grown vines of cv. Sultana on either their own roots or grafted to a range of rootstocks, were drip‐irrigated at one of three salinity levels (0.40, 1.75 and 3.50 dS/m) over a five year period. Rootstocks were Ramsey, 1103 Paulsen, J17‐69 and 4 hybrids (designated R1, R2, R3 and R4) derived from parentage involving Vitis champini, V. berlandieri and V. vinifera. Grape juice total soluble solids, titratable acidity and pH were measured at harvest, while colour of dried fruit was measured before and after processing and again after six months storage. Damage index (an indicator of skin damage) was measured post‐processing; sugar crystal formation in dried grapes and dried grape compaction were measured post‐storage. There was a strong salinity x rootstock interaction for grape juice soluble solids concentration, soluble solids yield (the product of soluble solids concentration and fruit yield) and pH, but not for titratable acidity when analysed on the basis of 5 year means. Small increases (< 5%) in juice soluble solids concentration were recorded at medium salinity (1.75 dS/m) for the low vigour genotypes, Sultana on own‐roots and on J17‐69 rootstock, based on the 5 year means and Fisher's protected (interaction) LSDs. Moderate increases (< 10%) also occurred in years of low crop load e.g. 1993 at high salinity for the high vigour rootstock R2 and in 1995 at medium salinity for Sultana on own roots and at high salinity for Sultana on R1 rootstock. By contrast, decreases in soluble solids concentration occurred with increasing salinity for the high vigour rootstocks (Ramsey, 1103 Paulsen and R2) in years of high crop load e.g. 1992. Small (< 2%) increases in grape juice pH were recorded at high salinity for Sultana on R3 rootstock and moderate increases (< 7%) in grape juice titratable acidity were recorded at high salinity for Sultana on own roots and Sultana on J17‐69, R1, R2 and R4 rootstocks. Dried grapes from all treatments achieved a light amber colour (quality grade termed 5 crown light) and were generally of high quality. Sultanas from own‐rooted grapevines were redder (higher ‘a‐value’) than sultanas from 1103 Paulsen and Ramsey when assessed as unprocessed fruit, after processing (both years) and after 6 weeks storage. While soluble solids yields per vine were 23–31% lower at high salinity for Sultana on own roots and on R1, R3 and R4 rootstocks, they were unaffected by high salinity for Ramsey, 1103 Paulsen and R2 rootstocks. Moreover, soluble solids yields for Sultana on Ramsey, 1103 Paulsen and R2 rootstocks were 1.4 to 2.5‐fold higher than for Sultana on the other rootstocks at high salinity. This study has shown that over a 5 year period rootstocks such as Ramsey, 1103 Paulsen and R2 grafted with Sultana were tolerant of salinity, producing dried grapes of generally high quality.     

Rootstock effects on salt tolerance of irrigated field-grown grapevines (Vitis vinifera L. cv. Sultana).: 1. Yield and vigour inter-relationships

Vegetative growth and yield of grapevines grown in the field on their own roots or grafted to a range of rootstocks were investigated under drip irrigation with water of three salinity levels (0.40, 1.75 and 3.50 dS/m) over a five‐year period. Rootstocks were Ramsey, 1103 Paulsen, J17–69 and 4 hybrids (designated R1, R2, R3 and R4) derived from parentage involving Vitis champini, V. berlandieri and V. vinifera. Of measured yield components (bunches per shoot, bunches per vine, weight per bunch, weight per berry and total yield), only weight per berry was significantly reduced by high salinity (3.50 dS/m) in each year of the trial with the exception of Sultana on 1103 Paulsen and R2 in 1991 and Sultana on Ramsey in 1993. Weights of one‐year‐old pruning wood were also reduced by high salinity in all years for own roots and all rootstocks, with the exception of R2. Mean yield values at each salinity level over the five‐year period of the trial were highest for Sultana on Ramsey, 1103 Paulsen and R2. High salinity had no effect on five‐year mean yields of Sultana on Ramsey, 1103 Paulsen and R2. Yield (five‐year means) of Sultana on Ramsey and R2 at 1.75 dS/m were significantly higher than at 0.40 dS/m by 14.6% and 13.4% respectively. In contrast, 5‐year mean yields of Sultana on J17–69, own roots, R1, R3 and R4 at 3.50 dS/m were reduced by 54, 30, 20, 30 and 30% respectively. Yield of Sultana on J17–69, R1 and R4 rootstocks was reduced by 47, 20 and 24% respectively at 1.75 dS/m. When yield was regressed against bunches per vine and weight per bunch for Sultana on own roots and on Ramsey rootstock, bunches per vine was the main determinant of yield, while weight per berry showed a poor correlation with yield at all salinity levels. Rootstock ranking for salt tolerance based on yield at high salinity was the same as rankings for pruning wood weights at high salinity. The same occurred at medium salinity, demonstrating that vigour imparted by the rootstock was a major factor in Sultana salt tolerance as measured by yield. Mean root weighted soil saturation paste electrical conductivities (RWEC_e) (determined from soil saturation paste salinities and root length densities) were in the range 2.0–2.6 dS/m for the low salinity treatment, increasing to approximately 5.4 dS/m with increasing salinity of irrigation water. Yield reduction for own‐rooted vines for each 1.0 dS/m increase in RWEC_e above 2.6 dS/m was 9.3%.     

Rootstock response of Shiraz (Vitis vinifera) grapevines to dry and drip-irrigated conditions

The yield and growth of Vitis vinifera cv. Shiraz scions grafted onto seven rootstocks were compared with that of own-rooted vines in a shallow sandy soil that was free of nematodes and phylloxera in the Barossa Valley of South Australia. In the absence of irrigation, own-rooted vines yielded as much fruit as vines grafted on Ramsey, the highest-yielding unirrigated rootstock-scion combination. Scions grafted to the rootstocks 110R, K51–40, 1103P and Freedom yielded poorly without irrigation. The yield and growth of all vines increased significantly when a small volume of irrigation water (40–160 mm/annum) was applied; however, only scions grafted to Ramsey and Freedom rootstock yielded more fruit than own-rooted vines.     

Interaction of rootstock and applied water amounts at various fractions of estimated evapotranspiration (ETc) on productivity of Cabernet Sauvignon

Background and Aims: It is commonly thought that grapevine rootstocks vary in their tolerance to drought. This study examined the interaction between various applied water amounts and productivity of Cabernet Sauvignon grafted onto five rootstocks. Methods and Results: The commercial vineyard used in this study was located along the central coast of California. The rootstocks used were Teleki 5C, 110 Ricter, 140 Ruggeri, 1103 Paulson and Freedom. Irrigation amounts ranged from 0.25 up to 1.25 of estimated vineyard evapotranspiration. Midday leaf water potential (Ψ_l), was significantly affected by irrigation treatment but not by rootstock. There was a significant effect of irrigation treatment and rootstock on berry weight, number of bunches per vine and yield but no interaction between those two factors. The rootstock 5C had the lowest yield compared with the other rootstocks. Yield at the 0.25 irrigation level was approximately 62% of the yield at the 1.25 irrigation level across rootstocks. Irrigation treatment was the only factor that significantly affected soluble solids in the fruit. There was a significant interaction between rootstock and irrigation amount on pruning weights. Berry weight, yield and pruning weights were linearly correlated with midday Ψ_l across rootstock and year. Conclusions: The results indicate that the rootstocks producing greater yields at the highest applied water amounts also produced greater yields when deficit irrigated. Significance of the Study: Under both stressed and non-stressed conditions, the rootstocks with the highest yield were those with the greatest number of bunches.     

An assessment of rootstocks for Sunmuscat (Vitis vinifera L.): a new drying variety

Sunmuscat scions, either grafted onto one of seven rootstock, or as own-rooted vines, were grown under irrigation according to the practices of warm-climate viticulture in north-western Victoria. The trial was located within a commercial vineyard on a sandy loam soil, and represented a typical replant situation. Grapevine performance was assessed over five seasons, viz. 1999–2004 inclusive, in terms of yield per vine, berry weight, juice composition and vigour (based on trunk girth). The highest yielding rootstock over the trial period was 1103 Paulsen (28.9 kg /vine) followed by 140 Ruggeri and Ramsey (26.1 and 25.8 kg /vine respectively), S04 (22.5 kg /vine), Schwarzmann, 101-14 and Teleki 5A (19.9, 18.7 and 18.4 kg /vine respectively). Scions on their own roots returned lowest yield (15.5 kg /vine). Berry weights were largest for the three high yielding rootstocks (2.3 g) and smallest with own roots (2.0 g). Total soluble sugars in harvested fruit were largely unaffected by rootstock in most seasons, although taken over all seasons, fruit from scions grafted onto Ramsey rootstock had the lowest levels, while Teleki 5A had the highest levels (viz. 23.4^oBrix and 24.7^oBrix respectively). Comparative vigour for all seven graft combinations was inferred from trunk circumference. There was a 2-fold difference in rootstock girth below the graft union (viz. 265 mm for 1103 Paulsen, compared to 135 mm for S04), whereas differences in the scion girth above the graft union were minor (only 16%). Relative compatibility of scion and stock was inferred from 'girth ratio' of trunk circumference above compared with below the graft union. Girth ratio was highest for Sunmuscat scions grafted onto SO4 rootstock, and lowest for scions on 1103 Paulsen. Scion girth and vine yield were broadly correlated.     

Grapevine canopy response to a high‐temperature event during deficit irrigation

Background and Aims: Despite much anecdotal evidence, there has been little scientific investigation of the potential effects of a high‐temperature event on grapevines during deficit irrigation. This study examined the interaction between temperature and water status on leaf physiology. Methods and Results: Two experiments used Cabernet Sauvignon cuttings grown in a glasshouse at approximately 27–30°C before deficit irrigation treatments were imposed. When water stress was apparent, a 2‐day high‐temperature event with maximum daytime temperatures of approximately 40–45°C was generated. Leaf damage, stomatal conductance and water potential of deficit‐irrigated vines were all affected to a greater extent than in the well‐watered vines. Conclusions: The negative effects of a high‐temperature event on vine physiology were more severe in vines experiencing water stress than in well‐watered vines, but recovery was rapid even without re‐watering. Significance of the Study: An increase in the use of deficit irrigation will lead to a greater likelihood of vines being water stressed on hot days, and the observed responses indicate that this will result in greater damage to the vine.     

Effects of novel hybrid and traditional rootstocks on vigour and yield components of Shiraz grapevines

Background and Aims:  The influence of grapevine rootstocks on vine vigour and crop yield is recognized as an integral part of viticultural management. However, the genetic potential of Vitis species rootstock hybrids for vigour and yield control is not fully exploited in Australian viticulture. The effect of 55 novel inter- and intra-species hybrids and five traditional hybrid rootstock cultivars on winter pruning weight, berry size and fruit yield of grafted Shiraz vines is presented. The genetic predictions that resulted from this analysis were used to illustrate how rootstocks that best perform for a combination of traits may be selected.
Methods and Results:  The use of linear mixed models and residual maximum likelihood procedures took into account repeated measures and spatial variation within a large field trial (720 vines). Over 6 years of assessment, variation of up to 93.9% in winter pruning weight, 81.9% in fruit yield and 21.0% in berry weight between rootstocks was estimated.
Conclusions:  The effect of rootstock genotype accounted for marked differences in conferred pruning weight, berry weight and fruit yield from trial averages. Comparison of statistical analysis techniques illustrated that the choice of such techniques may influence the outcome of genetic selection from field trial data.
Significance of the Study:  Such quantification of the variation between vines in vigour, fruit yield and berry size due to rootstock genotype provides a framework for selection of well-performing genotypes for inclusion in advanced generations of the CSIRO vine rootstock breeding program.