大豆胚芽油提取工艺的研究

以大豆胚芽出油率为指标，石油醚为脱脂溶剂，通过单因素和正交试验对大豆胚芽索氏提取工艺进行了研究。结果表明：大豆胚芽制油的最佳提取工艺为：粒度30目，料液比1：13，回流时间5h。在此条件下，大豆胚芽出油率为10．50％。     

A COMPARATIVE STUDY OF IRON BIOAVAILABILITY IN RATS FROM SOYBEAN AND COTTONSEED PROTEIN ISOLATE DIETS

The purpose of this research was to determine the relative iron bioavailability from soybean and cottonseed protein isolates by using an iron depletion/repletion method. Iron stores of rats were depleted so that any iron introduced in the diet would be utilized at a maximum and linear rate for hemoglobin regeneration. A comparison of iron utilization from cottonseed and soy to the reference standard could then be made. Between 40 and 47% of the iron consumed from the soy diet was converted to hemoglobin iron. The cottonseed diets had a significantly lower (29 to 34%) conversion efficiency (P < 0.05) in comparison to casein but were not significantly different from the soy diets.     

反胶束技术萃取植物蛋白和油脂的研究进展

介绍了反胶束萃取技术萃取植物蛋白和油脂的原理和特点，并对该技术的国内外发展现状进行了全面、系统地论述，对今后反胶束技术的发展趋势进行了预测。     

地木耳蛋白质提取工艺及其稳定性的研究

对地木耳蛋白质的提取工艺及稳定性进行了研究，结果证明地木耳蛋白质可以采用水为溶剂的方法提取，提取最佳条件为pH=9，浓度为3％（w／w），温度为40℃-50℃。提取时间控制在1．5h。2h即可达到较好的收率，蛋白质的收率可达75％。随着pH值的下降，地木耳蛋白质的溶解度也随之下降，pH〈6时蛋白质沉淀明显。地木耳蛋白质的热稳定性较好，加热温度、加热时间均对地木耳蛋白质的稳定性有一定的影响。地木耳蛋白质提取物具有较高的营养价值，可更方便地应用于食品加工业，应用前景广阔。     

RAW SOYBEAN MEAL: IMPROVED PROTEIN QUALITY AFTER AQUEOUS ETHANOL EXTRACTION1

Extraction of raw soybean meal with 40% ethanol in water and a short autoclaving yields a residue which supports a growth rate 27% greater than that of conventionally autoclaved meal when fed to weanling male rats. This residue contains an average of 73% of the dry matter and 91% of the protein of the original raw meal.     

双相溶剂萃取技术提取酱油渣中油脂和大豆异黄酮的研究

利用正己烷-乙醇-水构成的双相溶剂萃取技术同时从酱油渣中提取油脂和大豆异黄酮,采用正交设计方法,考察温度、时间、乙醇浓度、乙醇用量、正己烷用量对油脂和大豆异黄酮提取率的影响,并用极差分析方法对正交试验结果进行分析。确定最优的提取条件：温度60℃、乙醇浓度80％、乙醇用量0．071g／mL、正己烷用量0．1g／mL、时间2h,在此条件下,油脂提取效率为99．2％,大豆异黄酮提取效率为94．5％。     

AQUEOUS ETHANOL EXTRACTION OF SOYBEAN TRYPSIN INHIBITORS AND CHARACTERIZATION OF A CALCIUM-SENSITIVE FRACTION

The trypsin inhibition by an 85% aqueous ethanol extract of soybeans was shown to have many similarities to that exhibited by long chain fatty acids and their acyl CoA esters, in terms of concentration dependence, time dependence and susceptibility to Ca⁺⁺ suppression. The heat-stable and hexane-extractable inhibitor in the extract was thus referred to as the Ca⁺⁺ -sensitive fraction, in contrast to classic proteinaceous inhibitors. Tempeh fermentation increased the antitryptic activity of the 85% ethanol extract. Extraction of both classic and Ca⁺⁺ -sensitive inhibitors from soybeans by aqueous ethanol was found to be concentration dependent. Aqueous solvents with less than 20% ethanol removed classic inhibitors as effectively as water, but extracted no Ca⁺⁺ -sensitive one. Above 20% ethanol in the solvent, the classic inhibitors in the extract decreased until reaching a zero value at 70% ethanol concentration, while the Ca⁺⁺-sensitive fraction increased to a maximum at 85%.     

大豆分离蛋白的提取及其改性方法

概述了大豆分离蛋白的传统提取法、膜分离法、泡沫分离法、双极膜法及其通常所用的改性方法 ：热处理法，酸处理和酶处理法。     

ENZYMATIC HYDROLYSIS OF SOYBEAN FOR SOLVENT AND MECHANICAL OIL EXTRACTION

Due to inefficient extractability of its low oil content, soybeans are often bypassed in village‐scale processing. Soygrits, flakes, and expanded collets were hydrolyzed by proteases, cellulases, and pectinases before oil extraction by solvent and static mechanical pressure. Driselase with multi‐enzyme activity and two proteases improved solvent extraction rates but only Driselase enhanced mechanical pressing. Up to 58% of seed oil was pressed from enzyme‐hydrolyzed flakes but 88% was pressed from Driselase‐treated collets. Either pretreatment is a feasible adjunct to mechanical pressing in small batch operations.     

蚕豆蛋白质提取工艺的研究

探讨蚕豆蛋白质提取影响因素,以单因素和正交试验确定最佳工艺条件.结果表明,蚕豆蛋白质的等电点PI为4.5,提取蚕豆中蛋白质最佳工艺条件为:温度40℃,提取时间2 h,料液比1:5,碱液浓度5×10-5mol/L.     

EXTRACTION YIELD OF SOLUBLE PROTEIN AND MICROSTRUCTURE OF SOYBEAN AFFECTED BY MICROWAVE HEATING

A laboratory‐scale microwave (MW)‐assisted extraction system was constructed and compared with a conventional shaking water bath regarding the extraction of soluble proteins in soybeans. Dynamic reaction was monitored by response surface methodology in the MW‐assisted extraction system. The yield of soluble protein increased until either temperature (T) or water/solid (W/S) ratio reached an optimum point (60.1C, 12.6 mL/g), and then decreased with further increase of T or W/S ratio. In addition, the yield of soluble protein increased with time within a range of 30 min, and no critical point was observed. The molecular mass of soluble protein was distributed from 19.3 to 81.3 kDa estimated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Scanning electron microscopy showed the destruction of the microstructure of soybean cells, which increased the extraction of soluble soy protein.     

大豆胚芽中异黄酮和皂甙同步提取工艺的研究

大豆异黄酮和大豆皂甙是大豆中的活性成分。本文主要研究了同时从大豆中提取这两种物质的工艺条件，通过溶剂对比实验、单因素实验确定了从大豆粕中提取大豆异黄酮和大豆皂甙的最佳工艺，并通过正交实验优化最佳生产工艺条件：温度55℃、固液比1：18、80％的乙醇溶液、提取时间2h、提取两次，异黄酮和皂甙得率分别为1．53％和4．07％。     

大豆胚轴中异黄酮的提取及其抗血小板聚集作用的研究

从大豆胚轴提取总糖苷后，采用C18反相柱层析法分离大豆异黄酮糖苷，再将其水解制备成异黄酮苷元，并研究大豆异黄酮对人血小板聚集性的影响。结果：在体外人血小板聚集实验中，大豆异黄酮苷元均抑制ADP、胶原蛋白和肾上腺素诱导的血小板聚集。提示：大豆异黄酮可能有助于防止血栓形成。