反相高效液相色谱法测定蛹虫草中虫草素的含量

建立了反相高效液相色谱法定量分析蛹虫草中虫草素(cordycepin)的方法。实验方法:色谱柱为Waters NOVAPAK C183.9mm×300mm,颗粒直径4μm;流动相为KH2PO4-K2HPO4缓冲液(0.01mol／L,PH=6.86)+1％四氢呋喃;流速为1.0mL／min,检测波长为260nm。在该实验条件下,虫草素的线性范围为3.23μg/mL-129.0μg／mL(r=0.9999),最小检出限为0.25ng。虫草素回收率平均值为97.5％,RSD为0.887％。该方法灵敏、快速、准确,适用于人工培植虫草中活性成分虫草素的检测。     

长白山地区高山红景天中红景天苷含量及农药残留的测定

为了解长白山地区高山红景天药材的质量,对产自长白山地区不同年限的人工栽培和野生高山红景天中的红景天苷含量和农药残留量进行了测定.在检测波长为275nm、色谱柱为PLATISTLTM C18柱(250mm×4.6mm,5μm)、流动相为甲醇-乙酸铵溶液、线性度(r)高于0.999 8、平均回收率为96.62%、相对标准偏差为1.32%的条件下,采用高效液相色谱法测定了红景天苷的含量;在色谱柱为HP-5毛细管气相色谱柱(0.32mm×30m×0.25μm)、进样口温度为250℃、Ni63电子捕获检测器温度为300℃、尾吹15mL/min、柱温以10℃/min升至220℃并保持20min条件下,采用气相色谱法测定了农药残留.结果表明,不同年限人工栽培品的红景天苷含量有差异,且含量均低于野生品,样品中均无六六六、滴滴涕农药残留.     

HPLC法测定大鼠血浆中蛇床子素的含量

为了测定大鼠血浆中蛇床子素的含量,以乙醚萃取法处理,采用高效液相色谱法对血浆中蛇床子素的含量进行测定。结果表明:在所采用的色谱条件和血浆处理方法下,蛇床子素在0.0125-0.4μg/mL范围内有很好的线性关系。该方法灵敏度高,精密度和准确度好,适用于蛇床子素的药代动力学与生物利用度研究。     

高效液相色谱法测定虫草黄酒中虫草素的研究

采用高效液相色谱法建立了虫草黄酒中虫草素含量的测定方法,探讨了虫草素提取条件,分析了流动相组成、柱温、波长对检测结果的影响.研究表明：色谱柱为Waters Symmetry C18柱,流动相为甲醇∶水=15∶85,流速为1mL/min;检测波长为288nm,柱温为40℃时分离、检测效果最好;方法检出限为0.05ng.该测定方法在黄酒质量控制方面具有应用价值.     

高效液相色谱法测定南、北五味子中木脂素含量

建立了用高效液相色谱法测定南、北五味子中5种木脂素成分(五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素和五味子乙素)的分析方法。以甲醇和水为流动相,采用梯度洗脱,对21个不同产地的南、北五味子样本进行了色谱分离,并利用标准物质、保留时间和紫外光谱对木脂素进行定性。结果表明,样品中各组分的色谱峰基本达到基线分离,该方法重复性好、灵敏度高,为评价南、北五味子中的木脂素活性成分提供了一种可靠的分析方法。     

高效液相色谱法测定南、北五味子中木脂素含量

建立了用高效液相色谱法测定南、北五味子中5种木脂素成分(五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素和五味子乙素)的分析方法。以甲醇和水为流动相,采用梯度洗脱,对21个不同产地的南、北五味子样本进行了色谱分离,并利用标准物质、保留时间和紫外光谱对木脂素进行定性。结果表明,样品中各组分的色谱峰基本达到基线分离,该方法重复性好、灵敏度高,为评价南、北五味子中的木脂素活性成分提供了一种可靠的分析方法。     

克喘素片剂中克化特罗含量的测定

提出用ＫｒｏｍａｓｃｌＣ１８柱,以ＰＨ为６．０含０．２ｍｏｌ／ＬｐＨ６．０ＨＡｃ－ＮＨ４Ａｃ缓冲溶液的水－甲醇（３５：６５,体积比）混合溶液作流动相,用外标法定量的反相ＨＰＬＣ测定克喘素片剂中克化特罗的含量,样品经溶解、过滤后直接进样,操作简便快速。克化特罗的测定线性范围为１．２－４２μｇ／ｍｌ,回收率为１０３．６％,相对标准偏差为２．２－２．４％。     

高效液相色谱法测定金丝桃素

采用高效液相色谱法测定了贯叶连翘提取物中金丝桃素的含量,用μ－Ｂｏｎｄａｐａｋ Ｃ１８为固定相,甲醇－乙酸乙酯－０．１ｍｏｌ／Ｌ磷酸盐缓冲液（ＰＨ２．１）（６０：２０：２０）为流动相,检测波长为５９０ｎｍ,平均回收率为９９．８％,ＲＳＤ为１．１％,最低检测浓度为０．０１１ｍｇ／Ｌ。     

HPLC法测定新益肾蠲痹丸中延胡索乙素含量

建立HPLC法测定益肾蠲痹丸中延胡索乙素的含量。采用Kromasil色谱注,流动相为甲醇-乙腈-1%醋酸铵缓冲液（pH=5.0±0.02）（10∶30∶60）,流速1.0ml/min,检测波长283nm。延胡索乙素在0.07776～1.5552ug（r=0.9999）范围内线性关系良好,平均回收率为99.6%。     

高效液相色谱测定葡萄酒中白藜芦醇含量

建立了高效液相色谱测定葡萄酒中白藜芦醇的方法。在C18为色谱柱,乙醇为溶剂,甲醇为流动相,流速为1 mL／min ,检测波长为306 nm的条件下,白藜芦醇的线性范围为0.2344～45μg／mL ,检测限为0.075μg／m L ,定量限为0.23μg／m L ,具有较好的精密度、稳定性和回收率。实验结果表明,该方法操作简便,分析快速,结果准确,可用于葡萄酒中白藜芦醇的定性、定量检测。     

长白山林区森林生态资源及可持续发展

分析了长白山林区森林生态资源的基本特征和利用中存在的问题,提出了可持续发展的基本措施。     

Modeling diameter distribution of the broadleaved-Korean pine mixed forest on Changbai Mountains of China

The broadleaved-Korean pine mixed forest is a native vegetation in the Changbai Mountains, northeast China. The probability density functions including the normal, negative exponential, Weibull and finite mixture distribution, were used to describe the diameter distributions of the species groups and entire forest stand. There is a strong correlation between parameters and mean DBH except the shape parameters in the mixture distribution. The diameter classes of species and entire forest stand showed not negative exponential but normal and “S” distribution. The mixture function was better than normal and Weibull to describe the model distribution. The location parameter had an effect on the estimated frequency in the first diameter class, when the estimated location parameter was bigger than the lower limit of the first diameter class.     

Modeling diameter distribution of the broadleaved-Korean pine mixed forest on Changbai Mountains of China

The broadleaved-Korean pine mixed forest is a native vegetation in the Changbai Mountains, northeast China. The probability density functions including the normal, negative exponential, Weibull and finite mixture distribution, were used to describe the diameter distributions of the species groups and entire forest stand. There is a strong correlation between parameters and mean DBH except the shape parameters in the mixture distribution. The diameter classes of species and entire forest stand showed not negative exponential but normal and "S" distribution. The mixture function was better than normal and Weibull to describe the model distribution. The location parameter had an effect on the estimated frequency in the first diameter class, when the estimated location parameter was bigger than the lower limit of the first diameter class.     

Dendroclimatic response of Picea jezoensis along an altitudinal gradient in Changbai Mountains

We investigated the effects of climate on Yeddo spruce (Picea jezoensis) radial growth along altitudinal gradients in the subalpine forests of Changbai Mountains using dendroclimatic analyses. Yeddo spruce at its lower and upper distribution limits was more sensitive to the climate. Despite precipitation being generally considered sufficient, we found that precipitation significantly affected Yeddo spruce radial growth. Yeddo spruce at its lower distribution limit was much more affected by precipitation while Yeddo spruce at its upper distribution limit was much more affected by minimum temperature. Yeddo spruce at its medial altitude was affected by sunshine ratio. These results demonstrated that climate affected Yeddo spruce growth differently depending on its altitudinal distributions in the Changbai Mountains. Both temperature and precipitation in the annu-alization period significantly correlated with Yeddo spruce radial growth. However, warmer signals were not reflected in radial growth trend during the past 20 years because annual total precipitation declined during the same period. It appeared that the climate affected tree rings growth by altering soil moisture availability.     

Dendroclimatic response of Picea jezoensis along an altitudinal gradient in Changbai Mountains

We investigated the effects of climate on Yeddo spruce (Picea jezoensis) radial growth along altitudinal gradients in the subalpine forests of Changbai Mountains using dendroclimatic analyses. Yeddo spruce at its lower and upper distribution limits was more sensitive to the climate. Despite precipitation being generally considered sufficient, we found that precipitation significantly affected Yeddo spruce radial growth. Yeddo spruce at its lower distribution limit was much more affected by precipitation while Yeddo spruce at its upper distribution limit was much more affected by minimum temperature. Yeddo spruce at its medial altitude was affected by sunshine ratio. These results demonstrated that climate affected Yeddo spruce growth differently depending on its altitudinal distributions in the Changbai Mountains. Both temperature and precipitation in the annualization period significantly correlated with Yeddo spruce radial growth. However, warmer signals were not reflected in radial growth trend during the past 20 years because annual total precipitation declined during the same period. It appeared that the climate affected tree rings growth by altering soil moisture availability.

     

长白山地区夏季旱涝时空分布规律研究

根据帕默尔旱度模式和刘巍巍等人修正的帕默尔旱度模式,以长白山地区20个气象站历年气温和降水资料建立长白山地区的旱涝指数模式,计算了能够反映长白山地区干湿变化的20个气象站1960-2005年夏季（6—8月）逐月水分距平指数,并应用经验正交函数展开方法分析了长白山地区夏季旱涝时空分布规律．结果表明：长白山地区夏季旱涝存在全区一致型、东北一西南反相型和西南、北、东部与中部反相型3种分布类型,其中全区一致干旱或一致雨涝是长白山地区夏季旱涝的最主要特征．     

长白山产中药材杜仲中痕量元素汞的测定

采用双通道原子荧光光谱法,测定吉林长白山产杜仲中元素汞的痕量.确定了仪器的最佳工作条件,在此条件下,汞的检出限为0.027 ng/mL,并测定出长白山产杜仲样品中汞的痕量,含量较低.本方法操作简单快速,基体干扰少,分析具有较高的灵敏度和准确度.     

长白山地区野生与栽培蓝靛果原花青素含量的比较

采用紫外分光光度法比较长白山地区野生与栽培蓝靛果果实原花青素的含量.首先使用回流提取法和大孔吸附树脂法得到原花青素粗品; 然后利用原花青素在280 nm处有最大吸收的特点,测定其在酸性条件下(pH=3)的吸光度.测定结果显示:原花青素含量在15.63～250.00 μg/mL之间呈现良好的线性范围; 野生品平均加样回收率为95.32%,RSD值为1.67%; 栽培品平均加样回收率为95.47%,RSD值为1.61%; 野生品原花青素含量为1.72%,栽培品原花青素含量为1.90%.该结果表明,栽培蓝靛果果     

长白山区桔梗和沙参中粗纤维、还原糖及蛋白质的测定

对长白山区桔梗和沙参中的粗纤维、还原糖及蛋白质进行了测定，实验结果表明：桔梗和沙参中的粗纤维、还原糖的含量较为接近，分别为5．16％、5．06％和1．94％、1．82％;而蛋白质的含量相差较大，分别为6．86％和8．38％。