短程生物脱氮和反硝化除磷的基础研究

本文采用SBR反应器,以制药废水为处理对象,针对制药废水高氯氮、高pH和高碱度的特点,充分利用废水中高浓度游离氨对硝酸菌的抑制作用,分别采用两种方法在常温下成功实现短程硝化反硝化。一种是在高温(27±1℃)环     

DO对短程同步硝化反硝化除磷工艺的影响

针对碳源偏低的城市污水,采用序批式活性污泥法研究DO对短程同步硝化反硝化除磷工艺的影响,同时对短程同步硝化反硝化和反硝化除磷的机理进行探讨.试验表明:控制DO浓度可在同一个反应器内既实现短程同步硝化反硝化反应又达到反硝化除磷的效果.综合考虑COD、NH4+-N、TN、TP的出水浓度达到一级A排放标准,得出最佳的DO控制...     

短程硝化反硝化A/O膜反应器处理矿区废水的研究

利用短程硝化反硝化A/O膜反应器来处理煤矿矿区生活污水和生产废水的混合污水,主要研究短程工艺的实现和脱氮效果。最佳工艺条件为曝气池DO为1.8 mg/L时,亚硝酸盐氮得到充分积累并且总氮去除率达到91.8%。     

DO对短程同步硝化反硝化除磷工艺的影响

针对碳源偏低的城市污水,采用序批式活性污泥法研究D0对短程同步硝化反硝化除磷工艺的影响,同时对短程同步硝化反硝化和反硝化除磷的机理进行探讨。试验表明：控制DO浓度可在同一个反应器内既实现短程同步硝化反硝化反应又达到反硝化除磷的效果。综合考虑COD、NHg—N、TN、TP的出水浓度达到一级A排放标准,得出最佳的D0控制范围。当D0浓度在0．5～1．0mg／LU时．COD的去除率达到93％～94％,Nil,＋一N的去除率为97％～98％,TN的去除率达到85％一96％,TP的去除率为91％～93％。     

短程硝化反硝化技术研究进展

综述了国内外短程硝化反硝化的技术进展。从短程硝化反硝化技术的影响因素、控制方式以及氨氧化菌的分子生物学研究等方面进行了分析,为在更普遍、更广泛的条件下实现短程硝化生物脱氮技术提供参考和支持。     

短程硝化反硝化生物脱氮技术的影响因素及工程应用

应用短程硝化反硝化反应处理氮肥企业排放的低碳氮比污水在经济上和技术上具有很大的应用价值。然而,诸多影响因素对这一反应的最终效果影响很大。通过查阅文献资料,总结了影响微生物短程硝化反硝化生命活动的主要因素;同时结合近期有关这一理论的工程应用情况,整理了不同短程硝化反硝化反应的工程实践结果,分析得到:亚硝化细菌在20℃时比生长速率最大,且随温度的升高而降低;反应的理想pH值应大于8.5;低DO条件下亚硝酸盐更容易得到积累。通过在工程上间接调整影响因素,可以有效控制微生物的反应类型,减少曝气量,节约运行成本。因此,讨论短程硝化反硝化反应的影响因素有利于深入开发具有较强适应性的污水处理工艺;同时,总结不同关于短程硝化反硝化反应的工程实践结果有利于更多工程应用的开发,解决更多高难废水的处理问题。     

氨氮废水处理过程中的好氧反硝化研究

采用序批式反应器处理氨氮废水 ,试验结果验证了好氧反硝化的存在 ,好氧反硝化脱氮能力随混合液溶解氧浓度的提高而降低 ,当溶解氧浓度为 0 5mg/L时 ,总氮去除率可达到 6 6 0 %。并结合理论分析 ,对好氧反硝化的机理进行了探讨     

潜流湿地短程硝化反硝化强化脱氮研究

为探索人工湿地系统短程硝化反硝化脱氮作用对微污染水体脱氮性能的提升效果及影响因素,构建两级垂直流潜流湿地系统,对比分析了间歇进水、同步回流、预曝气等运行方式下的净水效果及含氮污染物的转化规律,结果表明:进水低碳氮比条件下,系统对COD_Cr和NH■-N的去除率稳定在60.5%～64.3%和90%以上;通过水位/水量调节方式强化湿地内部自然富氧作用,实现TN去除效果增长8%～10%,亚硝化率呈升高趋势,NO■-N的累积现象有所改善;氨氧化细菌（AOB）和氨氧化古菌（AOA）群落分布一致性和优势群菌丰度显著提升,反硝化细菌（nirS）优势种群分布同时得到改善,水位/水量调控的运行方式可以提升短程硝化效率,为反硝化反应提供更充足的底质和溶解氧条件,强化湿地系统对微污染水体的短程硝化反硝化脱氮作用。     

晚期垃圾渗滤液短程硝化反硝化工艺特性研究

采用间歇式反应器(Batch Reactor,BR)研究了晚期垃圾渗滤液短程硝化反硝化工艺(SND)工艺特性.试验发现:在进水氨氮负荷约为0.27 gNH3—N/(L·d),温度约为27℃,pH控制在7.5时,该工艺DO浓度控制在1 mg/L时硝化效果较好.DO浓度从0.75 mg/L增加到1 mg/L时,氨氧化速率明显增加;继续再增加溶解氧浓度,氨氧化速率增加不明显.在整个过程中,亚硝酸盐积累率变化不大,维持在91％以上.当温度控制在25℃以上时,反应器处理效果较好.随着温度的下降,亚硝酸菌和反硝化菌活性降低,当温度低于25℃时,氨氧化速率和亚硝酸盐降解速率下降较快,曝气时间和出水亚硝酸盐氮浓度明显增加.     

SBBR自养型短程硝化的实现及其影响因素研究

采用序批式生物膜反应器(SBBR)以及模拟无碳源的理想生活污水,对系统内各参数进行反复调试,在一个月内实现了自养型短程硝化系统的启动,氨氮去除率为98%,亚硝态氮积累率为95.29%。当温度控制在20～35℃时都会实现短程硝化,而30℃时短程硝化的效果最佳;系统其他条件不变的情况下DO为1.5 mg/L时,短程硝化的效果最好;系统具有一定的承受有机负荷的能力,在进水中外加COD_(Cr)浓度不超过75 mg/L时,不会对系统产生影响且COD_(Cr)的去除率为50%。     

Optimisation of storage driven denitrification by using on-line specific oxygen uptake rate monitoring during SND in a SBR.

This study builds on previous experience of maximising the formation of COD as poly-hydroxybutyrate (PHB) and now describes a feedback technique of preserving the use of PHB for denitrification resulting in enhanced nitrogen removal rather than allowing its wasteful oxidation by oxygen. The feedback technique uses on-line SOUR monitoring for detecting the end-point of nitrification and controlling the aerobic phase length accordingly. The laboratory SBR was operated such that all organic substrate (acetate) was rapidly converted to PHB, which then served as the electron donor for nitrogen removal via simultaneous nitrification and denitrification (SND) during the aerobic phase (up to 70% SND). During SBR cycling with a fixed aeration length (240 minutes), PHB was unnecessarily oxidised after ammonium depletion, resulting in little denitrification and poor total nitrogen removal (69%). However, when the aerobic phase length was controlled via the SOUR, up to 1.8 CmM PHB (58 mg L(-1) COD) could be preserved, enabling improved total nitrogen removal (86%). The drop in the SOUR after ammonium depletion was a reproducible event that could be detected even when using raw wastewater and fresh activated sludge. The SOUR-control technique holds promise to build up PHB over a number of SBR cycles. While advanced oxygen-control is used for improved N-removal in several existing WWTPs, this study investigates the importance of oxygen control with relevance to PHB driven SND in sequencing batch reactors.     

The presence of ammonium facilitates nitrite reduction under PHB driven simultaneous nitrification and denitrification.

For economic and efficient nitrogen removal from wastewater treatment plants via simultaneous nitrification and denitrification the nitrification process should stop at the level of nitrite such that nitrite rather than nitrate becomes the substrate for denitrification. This study aims to contribute to the understanding of the conditions that are necessary to improve nitrite reduction over nitrite oxidation. Laboratory sequencing batch reactors (SBRs) were operated with synthetic wastewater containing acetate as COD and ammonium as the nitrogen source. Computer controlled operation of the reactors allowed reproducible simultaneous nitrification and denitrification (SND). The oxygen supply was kept precisely at a low level of 0.5 mgL(-1) and bacterial PHB was the only electron donor available for denitrification. During SND little nitrite or nitrate accumulated (< 20% total N), indicating that the reducing processes were almost as fast as the production of nitrite and nitrate from nitrification. Nitrite spiking tests were performed to investigate the fate of nitrite under different oxidation (0.1-1.5 mgL(-1) of dissolved oxygen) and reduction conditions. High levels of reducing power were provided by allowing the cells to build up to 2.5 mM of PHB. Nitrite added was preferentially oxidised to nitrate rather than reduced even when dissolved oxygen was low and reducing power (PHB) was excessively high. However, the presence of ammonium enabled significant reduction of nitrite under low oxygen conditions. This is consistent with previous observations in SBR where aerobic nitrite and nitrate reduction occurred only as long as ammonium was present. As soon as ammonium was depleted, the rate of denitrification decreased significantly. The significance of the observed strongly stimulating effect of ammonium on nitrite reduction under SND conditions is discussed and potential consequences for SBR operation are suggested.     

溶解氧对同步硝化反硝化脱氮影响研究

以实际生活污水为处理对象,利用生物膜内所具有的A/O环境,针对DO浓度对生物膜法同步脱氮效果影响进行试验研究.研究结果表明,在DO为2.5 mg/L时SND脱氮效果达最佳,TN去除率近70%;DO浓度过高或过低都不利于生物膜内部DO浓度梯度的形成,合理控制DO浓度,对生物膜法同步脱氮尤为重要.     

塔式生物滤池处理城市污水的研究

尽管目前塔式生物滤池在城市污水处理中运用较少 ,但在有可资利用的地形时它可达到少动力或无动力运行 ,有较大的优势。研究了塔式生物滤池的工作参数及其处理特点。     

同步硝化反硝化脱氮研究

在控制SBR反应器保持良好的好氧状态条件下 ,考察进水COD/NH3比值对同步硝化反硝化脱氮效率的影响。同时也对同步硝化反硝化机理进行了初步的探讨。研究表明 ,进水COD/NH3比值越高 ,总氮去除率越高 ,同步硝化反硝化现象越明显。由该试验可以推断活性污泥菌胶团中异养硝化菌和好氧反硝化菌的存在。     

Use of industrial wastewaters for the optimization and control of nitrogen removal processes.

In this experimental study the characterization of 2 industrial wastewaters, coming from an ice cream production industry (IW1) and a beet-sugar factory (IW2), with respect to their readily biodegradable fraction and denitrification potential, has been performed. To this end physical-chemical and biological characterization methods, both anoxic and aerobic, were used. Moreover a pilot scale SBR fed with municipal wastewater was started to verify the effect of the gradual addition of the concentrated organic wastewaters during the anoxic phase. The SBR was initially fed only with a primary municipal wastewater, then the organic load was increased by adding to the feed, during the anoxic phase, a small amount of the IW1 (second period). Once the initial conditions were restored the load was again raised using the second industrial wastewater (IW2) (third period). With those additions the nitrogen removal efficiency increased from 26% to 50%, in the case of the IW1 and from 23% to 53% in the case of the wastewater IW2, without any negative effect on the global performance of the system. In addition, periodical kinetic studies of denitrification and nitrification in the SBR, were performed.     

Nitrogen removal from pharmaceutical manufacturing wastewater with high concentration of ammonia and free ammonia via partial nitrification and denitrification.

In this study, laboratory-scale experiments were conducted applying a Sequencing Batch Reactor (SBR) activated sludge process to a wastewater stream from a pharmaceutical factory. Nitrogen removal can be achieved via partial nitrification and denitrification and the efficiency was above 99% at 23 degrees C+/-1. The experimental results indicated that the nitrite oxidizers were more sensitive than ammonia oxidizers to the free ammonia in the wastewater. The average accumulation rate of nitrite was much higher than that of nitrate. During nitrogen removal via the nitrite pathway, the end of nitrification and denitrification can be exactly decided by monitoring the variation of pH. Consequently, on-line control for nitrogen removal from the pharmaceutical manufacturing wastewater can be achieved and the cost of operation can be reduced.     

Enhanced nitrogen removal using C/N load adjustment and real-time control strategy in sequencing batch reactors for swine wastewater treatment.

The laboratory-scale sequencing batch reactor (SBR) was used to study the effectiveness of an integrated strategy of real time control with C/N ratio adjustment for practical swine wastewater treatment. Swine waste was used as the external carbon source for continuous treatment in the SBR reactors. Oxidation-reduction potential and pH were used as parameters to control the continuous denitrification and nitrification process, respectively. A constant effluent quality could be obtained, despite drastic variations in the characteristics of influent wastewater. Also, a relatively complete removal of nutrients was always ensured, since the optimum quantity of the external carbon source could be provided for complete denitrification, and a flexible hydraulic retention time was achieved by the successful real-time control strategy. The average removal efficiencies of total organic carbon and nitrogen were over 94% and 95%, respectively.     

A novel wastewater treatment process: simultaneous nitrification, denitrification and phosphorus removal.

Simultaneous nitrification and denitrification (SND) via the nitrite pathway and anaerobic-anoxic enhanced biological phosphorus removal (EBPR) are two processes that can significantly reduce the COD demand for nitrogen and phosphorus removal. The combination of these two processes has the potential of achieving simultaneous nitrogen and phosphorus removal with a minimal requirement for COD. A lab-scale sequencing batch reactor (SBR) was operated in alternating anaerobic-aerobic mode with a low dissolved oxygen concentration (DO, 0.5 mg/L) during the aerobic period, and was demonstrated to accomplish nitrification, denitrification and phosphorus removal. Under anaerobic conditions, COD was taken up and converted to polyhydroxyalkanoates (PHA), accompanied with phosphorus release. In the subsequent aerobic stage, PHA was oxidized and phosphorus was taken up to less than 0.5 mg/L at the end of the cycle. Ammonia was also oxidised during the aerobic period, but without accumulation of nitrite or nitrate in the system, indicating the occurrence of simultaneous nitrification and denitrification. However, off-gas analysis found that the final denitrification product was mainly nitrous oxide (N2O) not N2. Further experimental results demonstrated that nitrogen removal was via nitrite, not nitrate. These experiments also showed that denitrifying glycogen-accumulating organisms rather than denitrifying polyphosphate-accumulating organisms were responsible for the denitrification activity.     

Achieving and maintaining of short-cut nitrification in a cyclic activated sludge system

A lab-scale Cyclic Activated Sludge Technology (CAST) system was operated more than 5 months to evaluate the effects of the operation mode on nitrogen removal performance and investigate a feasible method for achieving short-cut nitrification in the system. Results showed that nitrogen was removed by conventional biological nitrification and denitrification in traditional operation mode (fill/aeration 2 h, settle 1 h, decant 1 h), whereas short-cut nitrification and denitrification was the main nitrogen removal pathway in modified operation mode and the nitrogen removal performance was enhanced. Short-cut nitrification was successfully achieved in CAST system at 17 ± 1 °C by adjusting operation conditions and the average total nitrogen removal efficiency increased by 11.4% compared to traditional mode. It was assumed that low dissolved oxygen (<1.0 mg/L) limitation combined with free ammonia (0.28-0.34 mg/L) inhibition on nitrite-oxidizing bacteria caused nitrite accumulation in modified mode. During maintaining period of short-cut nitrification, preset aeration time enhanced ammonium-oxidizing bacteria dominance. It was also found that low DO could result in overgrowth of filamentous microorganisms and poor sludge settleability. The pH variation could provide effective information for controlling aeration duration in modified mode. However, no evident breakpoint appeared on pH and DO profiles in traditional mode.