Effect of antioxidants on stabilization of meat products fortified with n-3 fatty acids

The effects of an n-3 oil emulsion, with and without added antioxidants, on lipid oxidation in n-3 polyunsaturated fatty acid (PUFA)-fortified meat products were studied. An emulsion of n-3 PUFAs was prepared (25% algal oil, 2.5% whey protein isolates, 10mM sodium citrate, 0.2% potassium sorbate, 500ppm of 70% mixed tocopherols, 100μM EDTA, pH 3, pasteurized at 75°C for 30min) and incorporated into fresh ground turkey, and fresh pork sausage (20% fat) to achieve a concentration of 500mg n-3 PUFA/110g meat. An antioxidant combination containing rosemary (0.2% w/w; radical quencher), citrate (0.5% w/w; sequestrant) and erythorbate (1g/kg product; reductant) was prepared and incorporated into ground turkey patties (5cm dia, 1.5cm thick) or fresh pork sausages (5cm dia, 1.5cm thick). Meat products were stored at 4°C or -18°C and analyzed for color (L*, a*, b* values), lipid oxidation (TBARS and lipid hydroperoxides) and n-3 PUFA profile. a* Values of refrigerated ground turkey patties decreased with storage, and an antioxidant combination effect was observed after 4 days (P<0.05). For fresh pork sausages at 4°C, control+antioxidant (CON+ANTI), and n-3+antioxidant (n-3+ANTI) groups showed greater a* values than controls (CON) indicating that the antioxidant combination stabilized meat color. TBARS and lipid hydroperoxides of both n-3 PUFA-enhanced meat products increased with storage (P<0.05); there were no significant changes in TBARS or lipid hydroperoxides for treatments containing the antioxidant combination (P<0.05). The actual level of n-3 PUFA incorporation in both meat products was greater than 87%; n-3 PUFA concentrations did not change within any treatment during storage (P>0.05). These results provide support for including antioxidant protection in n-3 PUFA fortified meat products.     

EFFECT OF ADJUNCTS ON THE COLOR STABILITY OF BOLOGNA AND FRESH BEEF SAUSAGES

Alpha tocopherol (a-T), sodium tripolyphosphate (STPP), ascorbic acid (AA), copper gluconate (CG), sodium suljite (SS), sodium erythorbate (SE), citric acid (CA) and tetrasodium pyrophosphate (TS) were added in various combinations to formulations of fresh beef sausage and bologna. Fresh beef sausages containing SS had higher (P < 0.05) Hunter a* values during storage under light at 4C. Bologna containing AA + CG had higher (P < 0.05) Hunter a* values, lower (P < 0.05) Hunter L* and b* values and hue angle, and a slower (P < 0.05) decline in a* and increase in b* values and hue angle during storage. Bologna containing AA + CG had a lower (P < 0.001) cook yield and higher (P < 0.05) compressive strength. Bologna containing phosphates had a higher cook yield and pH, and lower (P < 0.05) compressive strength.     

Effect of Sodium Erythorbate on Residual Nitrite, pH and Bacterial Growth in Liver Sausage Formulated with Different Levels of Mechanically Separated Pork

Some microbiological, chemical, and physical characteristics of liver sausage formulated with 0, 25, 50, and 75% mechanically separated pork (MSP), 156 ppm sodium nitrite and 0 or 550 ppm sodium erythorbate were studied during refrigerated storage (5°C, 6 days) and subsequent elevated-temperature holding (20–24°C, 48 hr). Growth of inoculated Clostridium sporogenes was inhibited by 156 ppm nitrite in all sausages even after 48 hr at 20–24°C, regardless of MSP level. Growth of aerobic mesophiles and facultative anaerobes at 20–24°C was also inhibited in sausage formulated with 156 ppm nitrite and 550 ppm erythorbate. Erythorbate addition and increased MSP levels resulted in higher Hunter a color values. Residual nitrite, pH values, and total iron content of liver sausage increased with increasing MSP content.     

Antioxidants to Preserve Rockfish Color

Thornyhead rockfish, Sebastolobus alascanus, were treated with mixed tocopherols, alone or plus ascorbic acid (TCAA), butylated hydroxytoluene (BHT) and sodium erythorbate to preserve red skin color during frozen storage. While all treatments significantly improved color retention compared to the control, at 4 mo the TCAA treated fish had significantly higher red color scores than any other treatment group, as measured using the CIE L*a*b* scale. The TCAA dip also appeared to enhance the red color compared to pre-treatment values.     

Effect of gamma radiation on refrigerated mechanically deboned chicken meat quality

Samples of mechanically deboned chicken meat (MDCM) were irradiated in the frozen form with doses of 0.0, 3.0 and 4.0 kGy, stored at 2±1?°C and evaluated for their sensory characteristics, color, thiobarbituric acid reactive substances (TBARS) and total psychrotrophic bacterial count for up to 12 days. The sensory analysis showed that volatile compounds associated with the odor irradiation produces, were dissipated from the samples of irradiated MDCM during storage and that the oxidation odor perceived in the samples irradiated with doses of 3.0 and 4.0 kGy was more pronounced (P<0.05) than in the non-irradiated samples, as from the 8th and 12th day of refrigeration, respectively, in agreement with the TBARS values. Irradiated MDCM showed higher values (P<0.05) for a(?) (redness) than the non-irradiated samples as from the 4th day under refrigeration. Considering the sensory analyses, color, psychrotrophic bacterial counts and TBARS analyses as a whole, the MDCM samples irradiated with doses of 0.0, 3.0 and 4.0 kGy were acceptable under refrigerated storage for 4, 10 and 6 days, respectively.     

Effect of carboxy-hemoglobin on color stability of cooked pork sausage

To investigate the effect of carboxy-hemoglobin (CO-Hb) on the color of cooked pork sausage (CPS), 6 treatments with 5 levels of CO-Hb (0, 0.2, 0.4, 0.6, and 0.8%) and 0.015% NaNO₂ were manufactured. Increased CO-Hb decreased a* values, but increased L* and b* values significantly (p<0.05). Color of the 0.4% CO-Hb samples had no significant change (p>0.05) during the 29-day storage. Both CO-Hb and carboxy-myoglobin (CO-Mb) were found in the pigments extracted from the 0.4% CO-Hb non-cooked sausages. Therefore, CO-Hb showed a potential color agent in the manufacture of sausages.     

Combined effects of potassium lactate and calcium ascorbate as sodium chloride substitutes on the physicochemical and sensory characteristics of low-sodium frankfurter sausage

The purpose of this study was to evaluate the combined effects of sodium chloride (NaCl) substitutes, including potassium lactate (K-lactate) and calcium ascorbate (Ca-ascorbate), on the physicochemical and sensory characteristics of low-sodium frankfurter sausage (1.2% content of NaCl). Sausages produced with 40% substitution of NaCl with combined K-lactate and Ca-ascorbate showed a higher value of lightness (P < 0.001) than sausages containing 2.0% content of NaCl (control). However, the sensory panels were unable to distinguish a difference in color intensity between the control and treatment groups. Frankfurter sausages produced with 30% K-lactate and 10% Ca-ascorbate exhibited similar water-holding capacity, textural properties, and organoleptic characteristics (P > 0.05) when compared to control sausages. Thus, the use of these salt mixtures is a good way to reduce the NaCl content in meat products while maintaining the quality of meat products. These results may be useful in developing low-sodium meat products.     

Effect of the gamma radiation dose rate on psychrotrophic bacteria, thiobarbituric acid reactive substances, and sensory characteristics of mechanically deboned chicken meat

Frozen samples of mechanically deboned chicken meat (MDCM) with skin were irradiated with gamma radiation doses of 0.0 kGy (control) and 3 kGy at 2 different radiation dose rates: 0.32 kGy/h (3 kGy) and 4.04 kGy/h (3 kGy). Batches of irradiated and control samples were evaluated during 11 d of refrigerated (2 ± 1 °C) storage for the following parameters: total psychrotrophic bacteria count, thiobarbituric acid reactive substances (TBARS), evaluation of objective color (L*, a*, and b*) and a sensory evaluation (irradiated odor, oxidized odor, pink and brown colors). No statistical difference (P > 0.05) was found amongst the TBARS values obtained for the MDCM samples irradiated with dose rates of 0.32 and 4.04 kGy/h. There was a significant increase (P < 0.05) in the psychrotrophic bacterial count as from the 7th day of refrigerated storage, for the MDCM samples irradiated at the dose rate of 4.04 kGy/h. With respect to the attribute of oxidized odor, the samples irradiated with a dose rate of 0.32 kGy/h showed a stronger intensity and were significantly different (P < 0.05) from the sample irradiated with a dose rate of 4.04 kGy/h on days 0 and 2 of refrigerated storage. Irradiation with a dose rate of 4.04 kGy/h (3 kGy) was shown to be the best condition for the processing of MDCM according to the evaluation of all the variables, under the conditions of this study. Practical Application: The results obtained for the application of different dose rates of ionizing radiation to mechanically deboned chicken meat will provide the food industry with information concerning the definition of the best processing conditions to maximize the sensory and food quality.     

The development of imitation crab stick containing chicken breast surimi

Imitation crab stick samples were made from Alaska pollack with Golden treadfin bream surimi (C), Alaska pollack with Golden treadfin bream surimi and spent laying hens breast surimi (T1), Alaska pollack with spent laying hens breast surimi (T2), and Alaska pollack with Golden treadfin bream with mechanically deboning chicken meat (MDCM) (T3). Crude protein was significantly lower in T2 and T3 compared with C and T1. However, crude fat and carbohydrate were not significantly different among the samples during storage periods. pH steadily increased until 2 weeks of storage thereafter pH was decreased in all samples. Lightness (L^∗) and whiteness (W) were significantly lower in T3 than those of other samples. In fatty acid composition, the amount of polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) was higher than other fatty acids in all samples at 0 week of storage period. TBARS (thiobarbituric acid reactive substances) value was highest in control sample compared with other samples. In sensory evaluation, color was the highest in C than other samples, whereas T3 was lowest in color than those of other samples. Overall acceptability was significantly highest in T1 compared with other samples during the storage periods.     

直接减盐法对哈尔滨红肠贮藏期间品质的影响

采用不同食盐添加量（2.00%、2.25%、2.50%、2.75%和3.00%）制备哈尔滨红肠，真空包装后于4℃下贮藏35 d，通过分析贮藏过程中红肠的理化指标、微生物指标以及感官品质等，探究直接减盐法对哈尔滨红肠品质以及贮藏特性的影响。结果表明，随食盐添加量的减少，哈尔滨红肠的pH值显著增加（P<0.05），水分质量分数和硫代巴比妥酸反应物（thiobarbituric acid reactive substances,TBARS）值呈增加趋势，a*值略有降低，b*值略有升高，但整体上没有显著性差异（P>0.05）。在贮藏末期（第35天），食盐添加量为3.00%处理组L*值显著低于2.00%处理组（P<0.05）。此外，在整个贮藏期间，高食盐添加量处理组（3.00%、2.75%和2.50%）的硬度和致密性相对较高，菌落总数较低。电子舌分析结果显示，随着贮藏时间的延长，不同食盐添加量的哈尔滨红肠酸味、咸味和回味B（苦回味）显著增强（P<0.05），鲜味显著减弱（P<0.05）。同时与2.00%处理组相比，2.50%和3.00%处理组风味特征更加相似。感官评...     

Evaluation of Milk Mineral Antioxidant Activity in Beef Meatballs and Nitrite-cured Sausage

ABSTRACT: The objective of this study was to determine the antioxidant activity of 1.5% milk mineral (MM) added to uncured cooked beef meatballs and to evaluate possible synergistic effects of MM in combination with 20-ppm or 40-ppm sodium nitrite in beef sausages. All treatments were also formulated with 1.5% salt and 10% added water. Thiobarbituric acid (TBA) values and Hunter color values were determined at 1 d, 8 d, and 15 d of storage at 2°C. Meatball cooked yield was also measured. Cooked yield was not different (P < 0.05) between control meatballs and those containing MM. As expected, treatments containing nitrite had higher redness ( CIE a* ) than samples without nitrite. Redness values increased with storage time in sausages containing 40-ppm nitrite. However, redness values decreased (P < 0.05) during storage for control meatballs, associated with increased lipid oxidation (higher TBA values). Lipid oxidation was lower ( P < 0.05) in samples containing 1.5% MM with TBA values <1.2 after 15 d of storage compared with 6.1 for control samples. There was no synergistic inhibition of lipid oxidation in samples containing 20-ppm or 40-ppm sodium nitrite plus 1.5% MM. Milk mineral alone at 1.5% of meat weight was sufficient for inhibition of lipid oxidation in cooked beef samples.     

Application and assessment of extruded edible casings manufactured from pectin and gelatin/sodium alginate blends for use with breakfast pork sausage

Casings formed from pectin (PN) containing 2.5% and 5% corn oil (CO) and olive oil (OO) and gelatine/sodium alginate blends (GSAB) containing 2.5% CO and OO respectively were used for sausage manufacture. Mechanical properties and water content of casings were assessed prior to application. Following sausage manufacture, product quality and shelf-life evaluation were assessed in terms of sensory attributes, instrumental colour, moisture loss and lipid oxidation. All manufactured casings were of good quality and initially produced intact and stable sausage products. However, with time, shrinkage of products occurred where GSAB casings were used. Sensory analysis of sausages showed that PN casings were more preferred to GSAB casings for sausage manufacture. CIE colour analysis supported sensory evaluation. Water losses from sausages using GSAB casings were lower compared to sausages using PN casings due to GSAB casings having higher ability to trap and absorb water compared to PN casings. Lipid oxidation developed over time for all sausage products, however, sausages manufactured using GSAB casings containing both CO and OO at 2.5% had better oxygen barrier properties compared to sausages manufactured using PN containing the same concentration of emulsified oils. Sausages manufactured using casings containing OO were more prone to lipid oxidation than those using CO. Importantly, sausages manufactured using PN casings containing 5.0% CO were extremely stable to lipid oxidation over a six day storage period and significantly, were <1 on the TBARS numbers scale.     

Effects of vegetable juice powder concentration and storage time on some chemical and sensory quality attributes of uncured, emulsified cooked sausages

ABSTRACT:  Uncured, no-nitrate/nitrite-added meat products can be manufactured with vegetable juice powder (VJP) and a starter culture containing Staphylococcus carnosus , resulting in quality and sensory attributes similar to traditional cured products. The 1st objective of this study was to determine the effects of varying concentrations of VJP and incubation times (MIN-HOLD) on quality characteristics, including lipid oxidation, color, and cured meat pigment concentrations, of emulsified-frankfurter-style-cooked (EFSC) sausages over a 90-d storage period. The 2nd objective was to compare residual nitrate and nitrite content resulting from different processing treatments and the 3rd objective was to assess sensory properties of finished products. Four EFSC sausage treatments (TRT) (TRT 1: 0.20% VJP, 30 MIN-HOLD; TRT 2: 0.20% VJP, 120 MIN-HOLD; TRT 3: 0.40% VJP, 30 MIN-HOLD; TRT 4: 0.40% VJP, 120 MIN-HOLD) and a sodium nitrite-added control (C) were used for this study. No differences for lipid oxidation (TBARS) between any TRTs and C or over time were observed. No differences ( P > 0.05) for CIE L * values were found between TRTs. CIE a * and reflectance ratio values revealed that TRTs 2, 4, and C were redder than TRTs 1 and 3 at day 0. Trained sensory intensity ratings for cured aroma, cured color, cured flavor, uniform color, and firmness determined that all but TRT 1 were similar to C. These results indicate a longer incubation time (120 compared with 30 min) was found more critical than VJP level (0.20% or 0.40%) to result in products comparable to a sodium nitrite-added control.     

Antioxidant activity of carrot juice in gamma irradiated beef sausage during refrigerated and frozen storage

The antioxidant activity of carrot juice in gamma irradiated beef sausage was studied. Four batches of beef sausage were prepared, in which the first batch was formulated with water as the control. The other batches were formulated with unconcentrated carrot juice, carrot juice concentrated by 35% and 60%, respectively. Samples were irradiated at doses of 0, 3 and 4.5 kGy. Then the extent of oxidation in raw sausages was determined during refrigerated and frozen storage through the determination of peroxide value (PV) and thiobarbituric acid reactive substances (TBARS) for lipid oxidation and carbonyl content for protein oxidation. The raw sausages were sensory evaluated during storage for colour, appearance and odour, while the grilled samples were sensory evaluated for their colour, odour, taste, texture and juiciness post-treatment only. Irradiation and storage significantly increased the PV, TBARS and carbonyl content in the samples formulated with water. The carrot juice significantly decreased the oxidative processes in the samples proportionally to the juice’s concentration. Furthermore, the sausages that were formulated with carrot juice had a high acceptable sensory scores as compared with the control samples.     

发酵香肠乳酸菌筛选及其对产品品质的影响

利用香肠模型结合主成分分析（PCA），筛出了一株对香肠风味物质有显著提升的植物乳杆菌M-25(Lactobacillus plantarum,M-25)。将其添加到香肠中，以未接菌组作为对照，在川式香肠制作的第0、1、3、7、14 d对pH、Aw、色泽、过氧化值（POV）、硫代巴比妥酸（TBARS）以及挥发性风味物质进行了检测。应用偏最小二乘模型（PLS-DA），以VIP（投影中的变量重要性）>1,P<0.05作为筛选条件对香肠中的特征风味物质进行评价。结果表明，随发酵时间推移对照组和接种植物乳杆菌香肠的pH均先降低后缓慢上升；Aw显著降低（P<0.05）;POV和TBARS显著升高（P<0.05）；而色泽却无显著性差异（P>0.05）。与对照组相比，接种植物乳杆菌香肠的pH、Aw、POV和TBARS值均明显更低，且接种植物乳杆菌香肠中醇类、酮类和酯类物质均明显高于对照组，能明显提升特征风味物质（异戊酸、3-羟基-2-丁酮、苯乙醛和苯乙醇）的含量。综上所述，利用香肠模型筛选的植物乳杆菌M-25能有效提升香肠品质和风味特性。     

Effect of Antioxidants and Cooking on Stability of n-3 Fatty Acids in Fortified Meat Products

ABSTRACT: This study was carried out to determine the effect of processing and cooking on n-3 polyunsaturated fatty acid (PUFA) stability and to determine the efficacy of antioxidants (ANTI) to minimize lipid oxidation in cooked n-3 PUFA-fortified meat products. An emulsion of n-3 PUFAs (25% algal oil) was incorporated into ground turkey, pork sausages, or restructured hams (500 mg n-3 PUFA/110 g meat) with or without an "antioxidant cocktail" containing citrate (0.5% w/w), erythorbate (1 g/kg product), and rosemary (0.2% w/w). Ground turkey and pork sausages were frozen 2 d, then cooked to 71°C, and stored at 4°C for 2 d. Cooked, restructured hams were sliced, vacuum packaged, and stored at 4°C, or frozen and thawed with subsequent storage at 4°C. Treatments were CON (control), n-3 (n-3 PUFAs), CON + ANTI and n-3 + ANTI. Products were analyzed for color, lipid oxidation (TBARS and peroxide values), and n-3 PUFA profile. TBARS of n-3 PUFA-fortified treatments in ground turkey and pork sausages increased with storage ( P < 0.05); there were no changes in TBARS for CON + ANTI and n-3 + ANTI groups ( P > 0.05). For restructured hams nitrite curing appeared to delay lipid oxidation such that antioxidant treatment effects were unobservable ( P > 0.05). Overall recovery of n-3 PUFAs after heat processing was 69% to 85%, and there was no effect of storage on n-3 PUFA concentration in raw or cooked products ( P > 0.05). Sensory scores for n-3 treated restructured hams were lower than controls ( P < 0.05). Results suggested that cooking resulted in some losses of n-3 PUFAs in fortified meat products and that an "antioxidant cocktail" protected against lipid oxidation during subsequent storage in non-cured meat products.     

Practical use of β-carotene-loaded nanoemulsion as a functional colorant in sausages made from goat meat surimi-like material

The potential applicability of β-carotene-loaded nanoemulsion (CNE) as a natural colorant in non-smoked sausage made from goat meat surimi-like material (GMS) was elucidated. The effect of CNE content (0–30 g 100 g⁻¹) on the physicochemical characteristic, oxidative stability and β-carotene remaining during cold storage (4 °C) was determined. The higher the CNE, the greater the a*, b* and redness index with the lower L*, and colour likeness score. CNE content had a little impact on the moisture content, a_w and pH of sausages over storage period. However, the expressible drip, breaking force and deformation were largely influenced by the CNE content. All CNE-containing sausages displayed a superior oxidative stability to control. Among CNE-incorporated samples, the lowest lipid oxidation and β-carotene degradation were noticeable in the sausage with 10 g 100 g⁻¹ CNE. Thus, the CNE at 10 g 100 g⁻¹ was a promising functional colorant for emulsion sausage made from GMS.     

Quality and shelf life evaluation of fermented sausages of buffalo meat with different levels of heart and fat

Investigations were carried to study the effect of heart incorporation (0%, 15% and 20%) and increasing levels of fat (20% and 25%) on physicochemical (pH, moisture content and thiobarbituric acid, TBA number) and microbiological (total plate count and yeast and mold count) quality and shelf life of semi dry sausages of buffalo meat during refrigerated storage (4 °C). Different levels of fat significantly (p < 0.05) increased the pH of the sausage samples. However different levels of heart incorporation did not significantly (p < 0.05) affect pH, moisture content and TBA number of sausage samples. Fresh samples had pH, moisture content and TBA number in the range of 5.15–5.28, 42.4–47.4% and 0.073–0.134 respectively. Refrigerated storage significantly (p < 0.05) increased TBA number of control samples while storage did not significantly (p < 0.05) increase the TBA number of sodium ascorbate (SA) treated samples. Total plate counts of twelve sausage samples were f under the TFTC (too few to count) limit at the initial stage. Incorporation of different levels of heart and also increasing levels of fat did not significantly (p < 0.05) increase the log TPC/g values. Yeast and molds were not detected in twelve samples of semi dry fermented sausages in their fresh condition. Storage revealed that there was a consistent decrease in pH, and moisture content. Refrigerated storage significantly (p < 0.05) reduced both pH and moisture contents. TBA number and total plate counts and yeast and mold counts of controls were found to increase significantly (p < 0.05) during refrigerated storage. However, in SA treated sausage, only TPC and yeast and mold count significantly (p < 0.05) increased during refrigerated storage. Shelf life of the sausages was found to be 60 days under refrigerated storage (4 °C).     

Effects of Rosemary Extract and Sodium Lactate on Quality of Vacuum-packaged Ground Ostrich Meat

ABSTRACT Vacuum‐packaged ground ostrich meat patties containing 2% sodium lactate (SL), 0.2% rosemary extract as oleoresin (RE), or their mixture (MIX) were evaluated and compared with control for their storage stability at 3 ± 1 °C in the dark by measuring pH, 2‐thiobarbituric acid‐reactive substance (TBARS) values, sample color (CIE L*, a*, b*, Hue and Chroma), and microbiological content. The pH values of ostrich patties, ranging from 6.03 to 6.13, were not affected by treatment (P < 0.05). At 9 d of storage, TBARS concentration for control samples containing no additives was 1.64 mg malonaldehyde/kg meat. Addition of RE to the ground ostrich meat inhibited lipid oxidation during storage at 3 ± 1 °C (P < 0.05). TBARS values of SL‐added samples were lower than control samples (P < 0.05); addition of SL also delayed the oxidation. It was found that RE had a protective effect on color, whereas addition of SL decreased CIE a* values (P < 0.05). SL, either alone or with RE, was effective in inhibiting total aerobic bacteria (TAB), coliforms, lactic acid bacteria (LAB), and Brochothrix thermosphacta in ostrich patties (P < 0.05) and provided a 2‐log reduction in microbial population during storage. In addition, RE did not have a significant effect on microbial growth at the concentration used in this study.