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食用玫瑰茄红色素的稳定性研究 总被引:2,自引:0,他引:2
以玫瑰茄为原料,采用乙醇提取法,同时对玫瑰茄天然红色素的稳定性进行了较为系统的研究,试验表明:色素对光、热、氧化剂的稳定性差;色素对金属离子Na+,Mg2+表现出较好的稳定性;食用酸的存在使色素稳定性增强. 相似文献
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探讨了玛咖水提物抗前列腺增生的药效物质及其作用机理。将50只雄性ICR小鼠随机分为空白组、模型组、阳性药组,玛咖组(60 mg/kg,含苄基芥子油苷1.44 mg/kg)及苄基芥子油苷组(1.44 mg/kg)。除空白组外,其他组连续30 d皮下注射丙酸睾酮(5.0 mg/kg),制造小鼠前列腺增生模型,观察各组小鼠前列腺湿重、前列腺指数;血清中睾酮(T)、雌二醇(E2)、T/E2水平和前列腺酸性磷酸酶的影响及镜下病理学改变。结果显示,与模型组相比,玛咖组和苄基芥子油苷组小鼠前列腺湿重及前列腺指数显著降低(P0.01),血清T、DHT含量和T/E2比值明显降低(P0.05或P0.01),光镜下见前列腺增生程度大为减轻,增生的腺上皮乳头减少或消失,玛咖组的结果与苄基芥子油苷组相似。结果表明,玛咖水提物和苄基芥子油能显著抑制小鼠前列腺增生;苄基芥子油苷是玛咖水提取物抗前列腺增生主要的活性物质。 相似文献
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玫瑰茄提取物多酚含量与抗氧化作用研究 总被引:1,自引:0,他引:1
研究玫瑰茄的体外抗氧化活性。采用不同极性的有机溶剂萃取玫瑰茄40%乙醇提取物(粗提物),得到石油醚萃取物、氯仿萃取物、乙酸乙酯萃取物、正丁醇萃取物,然后采用Folin-Ciocalteu比色法测定粗提物以及各萃取物的多酚含量,同时应用DPPH法、试剂盒法、ABTS法分别测定粗提物和各萃取物的DPPH自由基清除能力、还原Fe3+能力以及ABTS自由基抑制能力。DPPH法、试剂盒法、ABTS法测定各提取物抗氧化能力的结果为粗提物>正丁醇萃取物>乙酸乙酯萃取物>氯仿萃取物>石油醚萃取物。玫瑰茄提取物具有较高的多酚含量和较强的抗氧化能力;玫瑰茄提取物的多酚含量与抗氧化能力之间存在较好的相关性。 相似文献
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目的探讨桑叶水提取物(mulberry leaf water extract,MWE)的降血脂功效,为桑叶保健品降血脂的开发利用提供依据。方法在饲喂高脂饲料的同时,给予小鼠不同剂量的桑叶水提取物,28 d后摘眼球取血,测定不同试验组小鼠血清血脂水平、载脂蛋白A1(Apo A1)和载脂蛋白B(Apo B)含量、小鼠肝脏中3-羟基-3-甲基戊二酰辅酶A还原酶(HMGR)、胆固醇-7α-羟化酶(CYP7A1)和卵磷脂胆固醇酰基转移酶(LCAT)活性和总胆汁酸(total bile acid,TBA)含量以及粪便含水率和TBA含量。结果与高脂组小鼠相比,MWE可降低高脂饮食小鼠肝脏系数及脂肪系数,并降低高脂饮食小鼠血脂水平,使肝脏HMG-COA和LCAT活性降低,提高CYP7A1活性,并抑制肝脏胆汁酸的积累,促进胆汁酸的排出。结论 MWE对高脂小鼠具有显著的降血脂功效,可有效减少脂肪积累,促进胆固醇代谢从而预防动脉硬化的发生。 相似文献
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玫瑰茄红色素的结构定性研究 总被引:8,自引:0,他引:8
对玫瑰茄红色素进行了一系列定性实验,欲对其结构进行初步鉴定。结果表明:玫瑰茄红色素属于花青甙类色素,有邻二酚羟基或邻三酚羟基,含有葡萄糖和木糖两种糖苷。其主体结构是失车菊素和飞燕草素。 相似文献
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本文论述了以玫瑰茄花萼为原料加工而成的纯天然营养保健饮料的生产工艺方法配方设计和产品性能。成份分析测定及医学临床应用等研究表明,玫瑰茄花萼营养丰富,并具有营养保健作用,提取浸汁后的残花,还可以制作玫瑰茄酱。 相似文献
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本实验以玫瑰茄花色苷为原料,研究了花色苷纯化的条件,以及添加不同稳定剂下花色苷溶液的热降解稳定性。结果表明:纯化花色苷的优化条件为上样浓度为600 mg/L,平衡3 h,上样体积为183 mL;洗脱剂为60%乙醇,洗脱流速为1 mL/min。纯化后的玫瑰茄花色苷冻干粉末,其色价为43.10±2.17,回收率为83.62%±5.72%,花色苷含量为216.50±1.83mg/g。添加1.0%海藻酸钠、羧甲基纤维素(Carboxymethylcellulose,CMC)和β-环糊精的三组玫瑰茄花色苷溶液在80、90和100℃三个温度下的降解均符合一级动力学方程,降解速率常数均随着温度的升高而增大,半衰期随着温度的升高而减小。β-环糊精具有很好的延缓花色苷降解的潜力,随着β-环糊精浓度的增加,花色苷的降解速率越来越小。80℃下加热150 min后,花色苷溶液中a*值减小,b*值增加,β-环糊精的浓度增加有利于维持花色苷的红度,其中1.5%β-环糊精组的护色效果最佳。 相似文献
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对使用大孔树脂吸附提取玫瑰茄红色素的条件和方法进行研究。通过7 种树脂对玫瑰茄红色素的吸附及不同洗脱剂的解吸比较表明:HPD-100 树脂对该色素有较好的吸附性能,用90% 的乙醇洗脱,使用20 次后其吸附性能无明显减弱,可循环使用。 相似文献
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Olvera-García V Castaño-Tostado E Rezendiz-Lopez RI Reynoso-Camacho R González de Mejía E Elizondo G Loarca-Piña G 《Journal of food science》2008,73(5):T75-T81
ABSTRACT: Hibiscus sabdariffa L. is used as a refreshing beverage and as a traditional medicine. The objective of this study was to determine the in vitro effect of phenolic compounds present in aqueous, ethyl acetate, and chloroform extracts of H. sabdariffa against mutagenicity of 1-nitropyrene (1-NP), and also the antiproliferative effect of these extracts. Inhibition of cell proliferation and DNA fragmentation were tested on transformed human HeLa cells. The hot aqueous extract (HAE) contained 22.27 ± 2.52 mg of protocatechuic acid (PCA) per gram of lyophilized dried extract, and was not statistically different from the cold aqueous or chloroform extracts; the ethyl acetate extract produced the least amount of PCA. The H. sabdariffa extracts inhibited mutagenicity of 1-NP in a dose-response manner. The inhibition rate on HeLa cells of HAE was also dose-dependent. The HAE did not induce DNA fragmentation. The results suggest that H. sabdariffa L. extracts have antimutagenic activity against 1-NP and decrease the proliferation of HeLa cells, probably due to phenolic acid composition. 相似文献
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Frank T Netzel G Kammerer DR Carle R Kler A Kriesl E Bitsch I Bitsch R Netzel M 《Journal of the science of food and agriculture》2012,92(10):2207-2218
BACKGROUND: To evaluate health benefits attributed to Hibiscus sabdariffa L. a randomized, open‐label, two‐way crossover study was undertaken to compare the impact of an aqueous H. sabdariffa L. extract (HSE) on the systemic antioxidant potential (AOP; assayed by ferric reducing antioxidant power (FRAP)) with a reference treatment (water) in eight healthy volunteers. The biokinetic variables were the areas under the curve (AUC) of plasma FRAP, ascorbic acid and urate that are above the pre‐dose concentration, and the amounts excreted into urine within 24 h (Ae0–24) of antioxidants as assayed by FRAP, ascorbic acid, uric acid, malondialdehyde (biomarker for oxidative stress), and hippuric acid (metabolite and potential biomarker for total polyphenol intake). RESULTS: HSE caused significantly higher plasma AUC of FRAP, an increase in Ae0–24 of FRAP, ascorbic acid and hippuric acid, whereas malondialdehyde excretion was reduced. Furthermore, the main hibiscus anthocyanins as well as one glucuronide conjugate could be quantified in the volunteers' urine (0.02% of the administered dose). CONCLUSION: The aqueous HSE investigated in this study enhanced the systemic AOP and reduced the oxidative stress in humans. Furthermore, the increased urinary hippuric acid excretion after HSE consumption indicates a high biotransformation of the ingested HSE polyphenols, most likely caused by the colonic microbiota. Copyright © 2012 Society of Chemical Industry 相似文献
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Purification,characterization and immunoregulatory activity of a polysaccharide isolated from Hibiscus sabdariffa L. 下载免费PDF全文
Daheng Zheng Ye Zou Samuel Jerry Cobbina Wei Wang Qian Li Yao Chen Weiwei Feng Yanmin Zou Ting Zhao Min Zhang Liuqing Yang Xiangyang Wu 《Journal of the science of food and agriculture》2017,97(5):1599-1606
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Liang‐Chih Liu Chau‐Jong Wang Ching‐Chih Lee Sheng‐Chi Su Huei‐Lin Chen Jen‐Dong Hsu Huei‐Jane Lee 《Journal of the science of food and agriculture》2010,90(2):329-337
BACKGROUND: Acetaminophen (AAP)‐induced oxidative stress can cause cell death to induce liver damage. The antioxidant effect of Hibiscus sabdariffa L. (HS) was shown in previous studies. In this study the effect of HS extract (HSE) on AAP‐induced liver injury in BALB/c mice was investigated. RESULTS: In vivo, BALB/c mice were fed orally with 200, 400 or 600 mg kg−1 HSE for 2 weeks and then injected with 1000 mg kg−1 AAP. Pretreatment with HSE decreased lipid peroxidation and increased catalase activity and glutathione level. It also decreased AAP‐induced liver injury, accompanied by decreased expression of pJNK, Bax and tBid in the liver. Additionally, HSE protected BALB/c normal liver cells from AAP‐induced damage in vitro. CONCLUSION: It has been demonstrated that HSE can protect the mouse liver from AAP‐induced injury and that the protective mechanism might involve decreasing oxidative stress and reducing cell death. Copyright © 2009 Society of Chemical Industry 相似文献
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Daniel Díaz‐Bandera Adriana Villanueva‐Carvajal Octavio Dublán‐García Baciliza Quintero‐Salazar Aurelio Dominguez‐Lopez 《International Journal of Food Science & Technology》2013,48(10):2150-2158
Gelatin beads containing a concentrated extract of Roselle (Hibiscus sabdariffa L.) calyx rich in polyphenolic compounds were coated with sodium alginate and ionotropically gelled using CaCl2. Single‐coated beads and double‐coated beads were obtained by this technique, and the release pattern of the loaded extract was evaluated. As a result, release pattern of these compounds fits properly to a first–order Weibull distribution equation. The release rate constant decreased linearly with the number of alginate coats and with the increase in immersion time in CaCl2 and the Lag period increased significantly with the number of alginate coats. The release of H. sabdariffa's polyphenols can be well controlled manipulating the number of alginate coats and the immersion time in a CaCl2 solution, allowing not only to control the gastrointestinal segment where they could be released but also to control the release rate with the certainty that the initial concentration will be completely released showing a highly significant antioxidant activity as well. 相似文献