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1.
High-yield diacylglycerol formation by solid-phase enzymatic glycerolysis of hydrogenated beef tallow 总被引:5,自引:0,他引:5
Tsuneo Yamane Sung Tae Kang Katsuyoshi Kawahara Yoshito Koizumi 《Journal of the American Oil Chemists' Society》1994,71(3):339-342
Diglyceride (DG) was prepared by reaction of hydrogenated beef tallow and glycerol in the presence of aPsesudomonas lipase. The yield of DG depended strongly on the reaction temperature. After initial incubation at 60°C for 2 h, followed
by the first temperature shift down to 55°C for 4 h and then the second shift down to 48°C for up to 3 d, the reaction mixture
became solid and a yield of approximately 90% DG was obtained. About 95% of total DG was 1,3-DG. The yield of DG was also
dependent on the glycerol (GL) to triglyceride (TG) molar ratio. At the molar ratio of 1∶2 (GL/TG), the enzyme-catalyzed reaction
was highly efficient and utilized essentially all of the glycerol. The free fatty acid (FFA) content at equilibrium depended
on the water concentration in the glycerol phase. The initial rate of FFA formation was low and was hardly affected by the
moisture content between 0.5 and 4%, but, at higher water content (4–6.7%), there was a small increase in the rate. 相似文献
2.
Diacylglycerol synthesis by enzymatic glycerolysis: Screening of commercially available lipases 总被引:1,自引:1,他引:1
Seven lipases were screened for their ability to synthesize DAG in the glycerolysis of rapeseed oil. In batch reactions with
free glycerol, the lipase carrier was of great importance for catalysis. Catalysis did not take place in reactions with lipases
having hydrophilic carriers. The best DAG yield (approx. 60 wt%) was achieved with Novozym 435 and Lipase PS-D after 7 h,
and an equilibrium was obtained. Stepwise addition of glycerol allowed catalysis with Novozym CALB L (immobilized) to take
place in spite of the hydrophilic carrier; however, the DAG yield was only 19 wt%. This result suggests that glycerol forms
a layer around the hydrophilic lipase particles, limiting contact between the lipases and the hydrophobic oil phase. With
glycerol absorbed on silica gel, all lipases catalyzed the glycerolysis reaction. Faster conversion of TAG was obtained with
Lipase PS-D, Lipase AK, and Lipase F-AP15 than in reactions with free glycerol, but the DAG yield remained approximately 60–65
wt%. Nonspecific lipases yielded more 1,3-DAG early in the reaction. 相似文献
3.
Yong Wang Mouming Zhao Shiyi Ou Keke Song Xue Han 《European Journal of Lipid Science and Technology》2009,111(7):652-662
Partial hydrolysis of palm olein catalyzed by phospholipase A1 (Lecitase Ultra) in a solvent‐free system was carried out to produce diacylglycerol (DAG)‐enriched palm olein (DEPO). Four reaction parameters, namely, reaction time (2–10 h), water content (20–60 wt‐% of the oil mass), enzyme load (10–50 U/g of the oil mass), and reaction temperature (30–60 °C), were investigated. The optimal conditions for partial hydrolysis of palm olein catalyzed by Lecitase Ultra were obtained by an orthogonal experiment as follows: 45 °C reaction temperature, 44 wt‐% water content, 8 h reaction time, and an enzyme load of 34 U/g. The upper oil layer of the reaction mixture with an acid value of 54.26 ± 0.86 mg KOH/g was first molecularly distilled at 150 °C to yield a DEPO with 35.51 wt‐% of DAG. The DEPO was distilled again at 250 °C to obtain a DAG oil with 74.52 wt‐% of DAG. The composition of the acylglycerols of palm olein and the DEPO were analyzed and identified by high‐performance liquid chromatography (HPLC) and HPLC/electrospray ionization/mass spectrometry. The released fatty acids from the partial hydrolysis of palm olein catalyzed by phospholipase A1 showed a higher saturated fatty acid content than that of the raw material. 相似文献
4.
5.
Cheong-Song Choi Jin-Woo Kim Cheol-Jin Jeong Huiyong Kim Ki-Pung Yoo 《The Journal of Supercritical Fluids》2011,58(3):365-370
Continuous transesterification of palm olein oil using supercritical methanol was investigated in the absence of a catalyst. The variables studied were reaction temperature (270–350 °C), pressure (20–40 MPa), and residence time (5–25 min), with a methanol-to-oil molar ratio of 40. Preheating at 245 °C was used to form a homogeneous phase in the absence of thermal decomposition of palm olein oil. The activation energies and reaction activation volumes of the fatty acid methyl ester (FAME), and those of the individual components (C16:0, C18:0, C18:1, and C18:2 methyl esters), were calculated. The entropies of activation (ΔS‡) of the transesterification reactions were also obtained. As the transesterification of vegetable oil in supercritical methanol included a strongly negative (−175 J/mol K) entropy of activation, transesterification required harsh conditions. 相似文献
6.
Jamshid Farmani Mohammad Safari Manouchehr Hamedi 《European Journal of Lipid Science and Technology》2006,108(8):636-643
The utilization of palm olein in the production of zero‐trans Iranian vanaspati through enzymatic interesterification was studied. Vanaspati fat was made from ternary blends of palm olein (POL), low‐erucic acid rapeseed oil (RSO) and sunflower oil (SFO) through direct interesterification of the blends or by blending interesterified POL with RSO and SFO. The slip melting point (SMP), the solid fat content (SFC) at 10–40 °C, the carbon number (CN) triacylglycerol (TAG) composition, the induction period (IP) of oxidation at 120 °C (IP120) and the IP of crystallization at 20 °C of the final products and non‐interesterified blends were evaluated. Results indicated that all the final products had higher SMP, SFC, IP of crystallization and CN 48 TAG (trisaturated TAG), and lower IP120, than their non‐interesterified blends. However, SMP, SFC, IP120, IP of crystallization and CN 48 TAG were higher for fats prepared by blending interesterified POL with RSO and SFO. A comparison between the SFC at 20–30 °C of the final products and those of a commercial low‐trans Iranian vanaspati showed that the least saturated fatty acid content necessary to achieve a zero‐trans fat suitable for use as Iranian vanaspati was 37.2% for directly interesterified blends and 28.8% for fats prepared by blending interesterified POL with liquid oils. 相似文献
7.
The cockle shell, which is available in abundance, has no any eminent use and is commonly regarded as a waste, was utilized as a source of calcium oxide in catalyzing a transesterification reaction to produce biodiesel (methyl esters). A central composite design (CCD) was used to optimize the two major influential reaction variables: catalyst and methanol amount towards purity and yield of methyl esters. The analysis of variance (ANOVA) indicated that the catalyst has a positive influence on purity but negative on the yield. Meanwhile, the methanol/oil mass ratio showed a positive effect on both purity and yield. Using CCD, the optimum reaction conditions were found to be 4.9 wt.% of catalyst and 0.54:1 methanol/oil mass ratio. The prepared catalyst was capable of being reused under the suggested optimal conditions. 相似文献
8.
Short‐path distillation (SPD) has been a technique used to purify products containing monoacylglycerols (MAG), diacylglycerols (DAG), etc. Palm oil and its fractions contain high contents of DAG, typically 5–8%, some of which have significant effects on the crystallization behavior of the fats. A possible way of reducing the DAG to lower levels using SPD is evaluated. Distillation of refined, bleached and deodorized palm olein was performed at different temperatures (220–250 °C) and flow rates (500 and 1000 g/h). Feed oil, residue oil and distillates were characterized in terms of composition and melting and cooling behavior. The DAG content of the feed oil was 6.5%. At high evaporating temperatures, the free fatty acid (FFA) concentration in the residue oil and the distillate oil decreased for the same flow rate. Increasing the feed flow rate while maintaining constant temperature led to a greater FFA concentration in both streams. The DAG content in the distillate increased at higher temperature, reaching 68% at 250 °C, while the residue oil achieved a level of 2.8% at lower flow feeding rates. Melting and cooling behavior were influenced by the composition of DAG and triacylglycerols. Thus, the distillate oils had higher melting profiles in contrast to the feed oil and the residue oil, which had similar profiles despite the removal of higher‐melting components. 相似文献
9.
Zaida Zainal Mohd Suria Affandi Yusoff 《Journal of the American Oil Chemists' Society》1999,76(9):1003-1008
Palm stearin (POs) and palm kernel olein (PKOo) blends were modified by enzymatic interesterification (IE) to achieve the
physical properties of margarine fats. POs and PKOo are both products of the palm oil industry that presently have limited
use. Rhizomucor miehei lipase (Lipozyme IM 60) was used to catalyze the interesterification of oil blends at 60°C. The progress of interesterification
was monitored by following changes in triacylglyceride composition. At 60°C interesterification can be completed in 5 h. Degrees
of hydrolysis obtained through IE for all blends were decreased from 2.9 to 2.0 by use of dry molecular sieves. The solid
fat contents of POs/PKOo 30:70 and 70:30 interesterified blends were 9.6 and 18.1 at 20°C, and 0 and 4.1 at 35°C, respectively.
The slip melting point (SMP) of POs/PKOo 30:70 was 40.0°C before interesterification and 29.9°C after IE. For POs/PKOs 70:30,
SMP was 47.7 before and 37.5°C after IE. These thermal characteristics of interesterified POs/PKOo blend ratios from 30:70
to 70:30 were comparable to those of commercial margarines. Results showed that IE was effective in producing solid fats with
less than 0.5% trans. 相似文献
10.
Roberta L. Krüger Alexsandra Valério Manuela Balen Jorge Luiz Ninow J. Vladimir Oliveira Débora de Oliveira Marcos Lúcio Corazza 《European Journal of Lipid Science and Technology》2010,112(8):921-927
In this work we report experimental data regarding the glycerolysis of olive oil using Novozym 435 in tert‐butanol organic system aiming at the production of monoacylglycerols (MAG) and diacylglycerols (DAG). Experiments were performed in batch mode, recording the reaction kinetics and evaluating the effects of temperature, enzyme concentration, tert‐butanol:oil/glycerol volume ratio and using solvent to substrates ratio of 1:1 and 5:1 v/v. Experimental results showed that lipase‐catalyzed glycerolysis in tert‐butanol might be a potential route for the production of high contents of MAG and DAG. The results also showed that it is possible to maximize the production of MAG and/or DAG, depending on the glycerol to oil molar ratio employed in the reactional system. Higher contents of MAG (53 wt%) and DAG (50 wt%) were achieved using glycerol to oil molar ratio of 3:1/6:1 and 0.5:1.5, respectively, both in 8 h of reaction at 70°C, 600 rpm and enzyme concentration of 10 wt%. 相似文献
11.
The effects of enzymatic transesterification on the melting behavior of palm stearin and palm olein, each blended separately
with flaxseed oil in the ratio of 90∶10 and catalyzed by various types of lipases, were studied. The commercial lipases used
were Lipozyme IM, Novozyme 435, and myceliumbound lipases of Aspergillus flavus and A. oryzae. The slip melting point (SMP) of the palm stearin/flaxseed oil (PS/FS) mixture transesterified with lipases decreased, with
the highest drop noted for the mixture transesterified with Lipozyme IM. However, when palm stearin was replaced with palm
olein, the SMP of the palm olein/flaxseed oil (PO/FS) mixture increased, with the commercial lipases causing an increase of
41 to 48% compared to the nontransesterified material. As expected, the solid fat content (SFC) of the transesterified PS/FS
was lower at all temperatures than that of the nontransesterified PS/FS sample. In contrast, all transesterified PO/FS increased
in SFC, particularly at 10°C. Results from DSc and HPLC analyses showed that the high-melting glycerides, especially the tripalmitin
of palm stearin, were hydrolyzed. Consequently, 1,3-dipalmitoylglycerol was found to accumulate in the mixture. There was
no difference in the FA compositions between the transesterified and nontransesterified mixtures. 相似文献
12.
Jamshid Farmani Mohammad Safari Manouchehr Hamedi 《European Journal of Lipid Science and Technology》2009,111(12):1212-1220
Binary blends of canola oil (CO) and palm olein (POo) or fully hydrogenated soybean oil (FHSBO) were interesterified using commercial lipase, Lypozyme TL IM, or sodium methoxide. Free fatty acids (FFA) and soap content increased and peroxide value (PV) decreased after enzymatic or chemical interesterification. No difference was observed between the PV of enzymatically and chemically interesterified blends. Enzymatically interesterified fats contained higher FFA and lower soap content than chemically prepared fats. Slip melting point (SMP) and solid‐fat content (SFC) of CO and POo blends increased, whereas those of CO and FHSBO blends decreased after chemical or enzymatic interesterification. Enzymatically interesterified CO and POo blends had lower SMP and SFC (at some temperatures) than chemically interesterified blends. The status was reverse when comparing chemically and enzymatically interesterified CO and FHSBO blends. The induction period for oxidation at 120°C of blends decreased after interesterification. However, chemically interesterified blends were more oxidatively stable than enzymatically interesterified blends. Interesterified blends of CO and POo or FHSBO displayed characteristics suited to application as trans‐free soft tub, stick, roll‐in and baker's margarine, cake shortening and vanaspati fat. 相似文献
13.
Nanjing Zhong Lin Li Xuebing Xu Ling‐Zhi Cheong Zhenbo Xu Dr. Bing Li 《European Journal of Lipid Science and Technology》2013,115(6):684-690
The present study aimed to produce MAG through low‐temperature chemical glycerolysis. Over 80% MAG yield with 97% TAG conversion was obtained within short reaction times at temperature of 35–55°C, when tert‐butanol (TB) or tert‐pentanol (TP) was used as reaction medium and sodium hydroxide (NaOH) as catalyst. TB gave a faster reaction rate than TP. Catalysts were important for the low‐temperature chemical glycerolysis reaction. Of the eight common base catalysts evaluated, only NaOH and potassium hydroxide (KOH) were effective, and NaOH was better than KOH. Reaction parameters were studied and optimized. The optimum conditions were TB dosage 3:1 (TB to oil in weight ratio), NaOH concentration 0.45 wt% based on oil, molar ratio of glycerol to oil 5:1. Under these conditions, similar MAG yield and TAG conversion was also observed by Novozym 435 catalyzed glycerolysis, however, a 4 h reaction was required. Practical applications: The process of NaOH catalyzed chemical glycerolysis for MAG production in TB solvent system described in this study provides several advantages including short reaction time and high product yield, which is potential for industrial considerations. 相似文献
14.
Solid phase enzymatic glycerolysis of beef tallow resulting in a high yield of monoglyceride 总被引:4,自引:0,他引:4
Gerald P. McNeill Shoichi Shimizu Tsuneo Yamane 《Journal of the American Oil Chemists' Society》1990,67(11):779-783
A mixture of mono-, di- and triglycerides was obtained when beef tallow was reacted with glycerol using lipase enzyme as a
catalyst. The reaction was carried out batchwise in a small vessel with agitation by magnetic stirring. The yield of monoglyceride
(MG) was greatly influenced by the reaction temperature—at higher temperatures (48–50°C) a yield of approximately 30% MG was
obtained, while at lower temperatures (38–46°C) a yield of approximately 70% MG was obtained. A sharp transition was observed
between the high and low yield equilibrium states. The temperature at which this transition occurred is called the critical
temperature (Tc) and was found to be 46°C in the case of tallow. During the course of the reaction, when approximately 40% MG had been synthesized,
the reaction mixture became solid but the reaction continued until approximately 70% MG had been synthesized.
A yield of 70% MG also was obtained with tallow at 42°C when a glycerol/tallow mole ratio ranging from 1.5 to 2.5 was used.
The free fatty acid content at equilibrium depended on the water concentration in the glycerol phase and varied from 0.5%
to 11.0% when the water content ranged from 0.6% to 12.5%. Above 8% water content, the yield of MG was reduced. Of the commercially
available lipases that were investigated, lipase fromPseudomonas fluorescens orChromobacterium viscosum resulted in the highest yield of MG. 相似文献
15.
Continuous production of fatty acids from palm olein by immobilized lipase in a two-phase system 总被引:1,自引:0,他引:1
Aran H-Kittikun Poonsuk Prasertsan Chatchai Sungpud 《Journal of the American Oil Chemists' Society》2000,77(6):599-603
Commercial lipases were tested for the ability to hydrolyze palm olein in isooctane in a two-phase system. Lipase OF (from
Candida rugosa) showed the highest specific activity of 209 U/mg protein where 1 U is the amount of lipase enzyme required to produce 1
μmol of fatty acid (as palmitic acid) per minute. The enzyme was adsorbed completely on Accurel EP 100 (particle size <200
μm) with 20.5% activity retained. The soluble and the immobilized lipase OF showed optimal activity at the same pH and temperature
(pH 6.5–7.5 and 35°C). However, the immobilized lipase had a wider range of pH and higher temperature stability. Continuous
hydrolysis of palm olein was performed in a packed-bed reactor with 656 U of immobilized enzyme. The substrate (20% palm olein
in isooctane) and Tris/maleate buffer were fed concurrently at the flow rates of 0.08 and 0.04 mL/min, respectively. The system
gave a degree of hydrolysis (DH) of 90–100% for up to 250 h. A more stable system allowing for more than 300 h operation at
DH>95% was achieved by mixing the immobilized enzyme with 1000–1500 μm Accurel EP100 to increase the system porosity and continuous
feeding of the aqueous phase recycling from the product mixture. A similar result was also obtained using 1007 U of the immobilized
enzyme and 60% palm olein in isooctane fed at 0.06 mL/min. 相似文献
16.
Porcine pancreas lipase (PPL) immobilized on silica particles with different size were prepared and employed successfully for ring-opening co-polymerization of 5-benzyloxy-trimethylene carbonate (BTMC) with 5,5-dimethyl-trimethylene carbonate (DTC) for the first time. Three kinds of silica particles with different sizes (150-250, 75-150 and 1 μm) were selected as carriers for enzyme immobilization. The structure of copolymers were confirmed by 1H and 13C NMR which showed no decarboxylation occurrence during the polymerization. The (Mn) of poly(BTMC-co-DTC) decreased rapidly with the increasing of immobilized PPL concentration. The carrier size of immobilized PPL affected both the catalytic activity and the polymer yield. The highest molecular weight (Mn=26,400) of poly(BTMC-co-DTC) was obtained at around 0.1% concentration of immobilized PPL on silica particles with size of 75-150 μm. 相似文献
17.
Fourier transform infrared (FTIR) spectroscopy with transmission cell is described to predict anisidine value of palm olein.
The calibration set was prepared by mixing the thermally oxidized palm olein and the unoxidized palm olein with certain ratios
(w/w) covering a wide range of anisidine values. A partial least square (PLS) regression technique was employed to construct
a calibration model. This model was further accomplished by a validation step. The standard error of prediction found was
0.51. The precision of this method was shown to be comparable to the accuracy of the American Oil Chemists’ Society method
used for measurement of anisidine value, with coefficient of determination (R
2) of 0.99. The study showed that mid-band FTIR spectroscopy combined with a PLS calibration technique is a versatile, efficient,
and accurate technique for the estimation of anisidine value of palm olein within about 2 min with less than 2 mL of sample. 相似文献
18.
Sayed Amir Hossein Goli Miskandar Mat Sahri Mahdi Kadivar 《European Journal of Lipid Science and Technology》2008,110(12):1102-1108
Structured lipids containing conjugated linoleic acid as a functional ingredient were blended with palm stearin in the ratios of 30 : 70, 40 : 60, 50 : 50, 60 : 40 and 70 : 30 (wt/wt). The blends were subjected to enzymatic interesterification by Candida antarctica lipase. After interesterification of the blends, changes in the physical properties of the products, including lower melting points and solid fat contents along with different melting behaviors, were evidenced. Analysis of triacylglycerols (TAG) of the interesterified blends showed a decrease in the concentration of high‐melting TAG. X‐ray diffraction analysis revealed, that all the reacted blends were predominantly in the β' crystal form. The mixture could be used for the formulation of margarines or other, similar products. 相似文献
19.
Y. B. Che Man M. H. Moh F. R. van de Voort 《Journal of the American Oil Chemists' Society》1999,76(4):485-490
A rapid direct Fourier transform infrared (FTIR) spectroscopic method using a 100 μ BaF2 transmission cell was developed for the determination of free fatty acid (FFA) in crude palm oil (CPO) and refined-bleached-deodorized
(RBD) palm olein, covering an analytical range of 3.0–6.5% and 0.07–0.6% FFA, respectively. The samples were prepared by hydrolyzing
oil with enzyme in an incubator. The optimal calibration models were constructed based on partial least squares (PLS) analysis
using the FTIR carboxyl region (C=O) from 1722 to 1690 cm−1. The resulting PLS calibrations were linear over the range tested. The standard errors of calibration (SEC) obtained were
0.08% FFA for CPO with correlation coefficient (R
2) of 0.992 and 0.01% FFA for RBD palm olein with R
2 of 0.994. The standard errors of performance (SEP) were 0.04% FFA for CPO with R
2 of 0.998 and 0.006% FFA for RBD palm olein with R
2 of 0.998, respectively. In terms of reproducibility (r) and accuracy (a), both FTIR and chemical methods showed comparable results. Because of its simpler and more rapid analysis, which is less
than 2 min per sample, as well as the minimum use of solvents and labor, FTIR has an advantage over the wet chemical method. 相似文献