共查询到18条相似文献,搜索用时 62 毫秒
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非离子表面活性剂双水相萃取氨基酸研究 总被引:2,自引:0,他引:2
该文对氨基酸萃取液在非离子表面活性剂双水相体系中的萃取分配相行为进行研究。采用脂肪醇聚氧乙烯醚AEO–7/盐双水相萃取L–苯丙氨酸,考察了加盐(NaCl、Na2SO4、Na3PO4)、AEO–7含量、萃取时间、加入萃取物L–苯丙氨酸的含量以及萃取温度对双水相及萃取分离L–苯丙氨酸的影响。结果表明,AEO–7/Na3PO4双水相萃取L–苯丙氨酸的适宜条件是当AEO–7的体积浓度为8%,Na3PO4·12H2O的质量浓度为85 g/L,温度为40℃,L–苯丙氨酸的质量浓度为0.532 8 g/L,时间为60 min时L–苯丙氨酸的萃取率可以达到98.7%,分配系数为15.3。 相似文献
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双水相萃取技术在食品工业中应用 总被引:2,自引:0,他引:2
在食品工业,双水相萃取技术与传统萃取相比具有很多优势。该文介绍双水相萃取技术及其原理、特点,综述双水相萃取技术在食品工业中应用,并展望其应用前景。 相似文献
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采用新型双水相体系对桔皮中橙皮苷进行分离、纯化研究,考察了粗提物用量、pH、温度等参数对橙皮苷在两相间的分配及其回收率的影响。首先选取2种低分子有机物及3种无机盐分别构成双水相体系,用浊点法对其成相性能进行研究。选取其中成相性能较好的物质构成双水相体系,对橙皮苷的乙醇粗提物进行双水相分离,考察体系及其成相浓度对橙皮苷分配系数的影响。用所得最佳体系——丙酮-K2HPO4双水相体系对桔皮中橙皮苷进行分离。最佳条件下,橙皮苷的萃取收率可达96.69%。采用制备型HPLC对其进一步纯化,梯度洗脱时分离效果较好。经测定,双水相体系纯化后,橙皮苷样品纯度约为71.36%。经制备型HPLC精制所得橙皮苷纯度为96.09%。橙皮苷总回收率为83.54%。 相似文献
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Oscar Aguilar Marco Rito‐Palomares 《Journal of the science of food and agriculture》2010,90(9):1385-1392
The increasing interest of the biopharmaceutical industry to exploit plants as economically viable production systems is demanding the development of new downstream strategies to maximize product recovery. Aqueous two‐phase systems (ATPSs) are a primary recovery technique that has shown great potential for the efficient extraction and purification of biological compounds. The present paper gives an overview of the efficient use of ATPS‐based strategies for the isolation and partial purification of bioparticles from plant origin. Selected examples highlight the main advantages of this technique, i.e. scaling‐up feasibility, process integration capability and biocompatibility. An overview of the recent approach of coupling ATPSs with traditional techniques to increase bioseparation process performance is discussed. A novel approach to characterization protein from plants combining ATPSs and two‐dimensional electrophoresis (2‐DE) is introduced as a tool for process development. In the particular case of products from plant origin, early success has demonstrated the potential application of ATPS‐based strategies to address the major disadvantages of the traditional recovery and purification techniques. This literature review discloses the relevant contribution of ATPSs to facilitate the establishment of bioprocesses in the growing field of high‐value products from plants. Copyright © 2010 Society of Chemical Industry 相似文献
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采用水酶法提取胡麻籽油并对其工艺条件进行了详细研究。在单因素实验的基础上,通过正交实验得出了最佳工艺条件,即20 g研碎油料,加酶量为0.10 g,酶解温度50℃,酶解时间1h,最适pH 5.4,料液比1∶10,浸提温度90℃,浸提时间9 h。另外,水酶法提取胡麻籽油的提油率比水浸法提取胡麻籽油的提油率高24.55%。 相似文献