Oxidative stability of virgin olive oil enriched with carnosic acid

The aim of this study was to evaluate the effect of the addition of carnosic acid on the oxidative stability of virgin olive oil. Two different amounts of carnosic acid (0.01 and 0.1 g/100 g oil) and two different temperatures (accelerated aging temperature, 60 °C; deep frying temperature, 180 °C) were considered. The influence of carnosic acid and heating time on the stability of the oils was studied by experimental design. The results obtained at 60 °C showed a dose dependent inhibition in the formation of primary and secondary oxidation products and a dose dependent enhancement of radical scavenging activity, which was only less significantly influenced by heating time. On the contrary, at 180 °C no protective effect against lipid oxidation was observed and the radical scavenging activity was practically zeroed by heating, probably as a consequence of a fast decomposition of carnosic acid.     

Characterization of crispness of French fries by fracture and acoustic measurements,effect of pre-frying and final frying times

The influence of pre-frying and final frying time on the crispness of French fries was evaluated by simultaneous analysis of the fracture and acoustic properties during instrumental simulation of human chewing. The analysis of the frequency distribution of the force and sound events corresponding to the crust of the French fry reflected an effect of both pre-frying and final frying time on instrumental crispness. Up to 60 s pre-frying, an increase in the number and magnitude of force and sound events was found, which reflect an increase in crispness. The increase in final frying time increased crispness, but it cannot counterbalance a lack of pre-frying. The moisture content of the whole French fries cannot explained the effect of pre-frying on crispness. Conversely, the effect of pre-frying can be explained when considering only the moisture content of the crust. Pre-frying may allow an easier loss of water from the crust during final frying, which will enhance its crispness.     

Acrylamide content and color development in fried potato strips

Acrylamide formation and changes in color of fried potato strips was investigated in relation to frying temperature and three treatments before frying. Potato strips (0.8 × 0.8 × 5 cm) of Bintje variety were fried at 150, 170 and 190 °C until reaching moisture contents of ∼40 g water/100 g (total basis). Prior to frying, potato strips were treated in one of the following ways: (i) immersed in distilled water for 0 min (control), 60 min and 120 min; (ii) blanched in hot water at six different time–temperature combinations (50 °C for 40 and 80 min; 70 °C for 10 and 45 min; 90 °C for 3 and 10 min); (iii) immersed in a citric acid solution of 10 g/L for an hour; (iv) immersed in a sodium pyrophosphate solution of 10 g/L for an hour. Acrylamide content and color was determined in the potato strips after frying. Immersed strips in water for 120 min showed a reduction of acrylamide formation of 33%, 21% and 27% at 150, 170 and 190 °C, respectively, when they were compared against the control. Potato strips blanched at 50 °C for 80 min had the lowest acrylamide content when compared against strips blanched at different conditions and fried at the same temperature (135, 327 and 564 μm acrylamide/kg for 150, 170 and 190 °C, respectively). Potato strip immersion in citric acid solution of 10 g/L reduced much more the acrylamide formation after frying than the strip immersion in sodium pyrophosphate solution of 10 g/L (53% vs. 17%, respectively, average values for the three temperatures tested). Acrylamide formation decreased dramatically as the frying temperature decreased from 190 to 150 °C for all the pre-treatments tested. Color represented by the parameters L* and a* showed high correlations (r² of 0.79 and 0.83, respectively) with French fry acrylamide content.     

Heat transfer coefficients during deep fat frying of sweetpotato: Effects of product size and oil temperature

Heat transfer coefficient, h during deep fat frying of sweetpotato was determined from designed experiments. Samples of three different disc sizes were fried at four oil temperatures between 150 and 180 °C. Heat transfer coefficient estimation was based on heat energy balance between the oil and sample during frying. Maximum h was reached at the earlier stage of frying (80–120 s). The range of maximum h reached was 710–850 W/m² °C. h at latter period of frying (200–300 s) was 450–550 W/m² °C. h was found to vary directly with oil temperature but inversely with sample size (p <0.05).     

Acrylamide and 5-hydroxymethylfurfural formation during baking of biscuits: NaCl and temperature–time profile effects and kinetics

The present study aimed to investigate the effect of recipe and temperature–time on the formation of acrylamide and 5-hydroxymethylfurfural (HMF) during biscuit baking. Baking experiments were performed with biscuits of two different recipes, with and without NaCl, at 180 °C, 190 °C and 200 °C. Acrylamide and HMF reached highest concentrations at 200 °C for both recipes. The presence of NaCl in the biscuit formulation lowered acrylamide concentrations at 180 °C and 190 °C but not at 200 °C, and led to higher concentrations of HMF at all the tested temperatures. Sucrose hydrolysis was a key step in acrylamide and HMF formation during biscuit baking, even though a significant amount of acrylamide already had formed before the onset of sucrose hydrolysis. A lag phase was observed before sucrose hydrolysis occurred, which might depend on the melting of crystalline sucrose occurring at approximately 180 °C.A mathematical model based on the chemical reaction pathways was developed for the recipe with NaCl baked at 200 °C. The model described the chemical evolution during the last part of biscuit baking, and accurately predicted acrylamide and HMF content at the end of baking. The model showed the significant contribution of the reducing sugars to the formation of both acrylamide and HMF. The model could not be extended to the entire baking period because it was not possible to incorporate the lag phase observed before sucrose hydrolysis. The results reported in this study confirm that the kinetics of acrylamide and HMF formation in real food and dry systems may depend on the physical state of their precursors.     

Effect of incubation temperature and caseinates on the rheological behaviour of Kefir

The effect of incubation temperature and the addition of caseinates on the rheological behaviour of kefir was studied by using a pneumatic tube viscometer of novel design. The results indicated that the incubation time increased as the incubation temperature was reduced and the casein concentration was increased. Kefir samples incubated at 25 °C showed the highest values of viscosity, while the samples incubated at 30 °C exhibited the lowest viscosity. The addition of caseinates caused the viscosity of the samples to increase and their flow behaviour index values to decrease. Kefir samples incubated at 30 °C exhibited the highest flow behaviour index values.     

Effect of calcium lactate and heat-shock on texture in fresh-cut lettuce during storage

Textural and microstructural changes in fresh-cut lettuce were analysed over 12 days storage. The vegetable was treated with 120 ppm chlorine and with 15 g/L calcium lactate at room temperature (∼18–20 °C) and at 50 °C (heat-shock). Texturometer analysis showed that samples washed with calcium lactate had significantly (p > 0.05) higher crispness values than samples washed with chlorine. However the use of 50 °C treatment (heat-shock) gave better textural properties at the end of storage and significantly retarded the softening process, being in agreement with the sensorial results. Cryo-SEM micrographs showed a loss of turgor (shrinkage) of the tissue cells in the samples washed with chlorine, effect not so evident in calcium lactate treated samples. The use of heat-shock in combination with calcium lactate reduced this phenomenon better than the other two treatments. Pectin methyl esterase (PME), enzyme related to textural changes, showed higher activity in samples treated with calcium lactate at 50 °C. The combination of calcium lactate and 50 °C washing temperature maintained objective and sensorial textural properties of fresh-cut lettuce better than the calcium lactate or chlorine washing treatments at room temperature.     

Protective role of vacuum vs. atmospheric frying on PUFA balance and lipid oxidation

The effect of vacuum frying processing on proximate composition, fatty acid profile, oxidative status and sensorial properties of fish patties, was evaluated as compared with conventional (atmospheric) frying.Vacuum frying procedure was carried out at 80 mmHg (water boiling point: 42 °C) and oil temperature (107 °C) determined to obtain an equivalent thermal driving force (Δ65 °C) of that of the atmospheric frying conditions used (165 °C). Frying times of 2, 4, 6, 8 and 10 min were investigated.Vacuum frying significantly prevented degradation of EPA (Eicosapentaenoic acid) and DHA (Docosahexaenoic acid), reducing the polyene index and maintaining a lower ω6/ω3 fatty acid ratio in samples, while no significant differences with conventional frying in total oil content were observed. The use of vacuum also reduced formation of peroxides and carbonyl derivatives. Tocopherol levels decreased in all samples regardless of the frying conditions used, although vacuum-fried samples maintained higher tocopherol levels after processing. These samples also showed higher lightness and lower a* and b* values, which can be associated to lower non-enzymatic browning levels.These results support the applicability of vacuum frying technology for fish patties, since it prevents colour changes, improves juiciness and reduces oxidation when compared to conventionally (atmospheric) fried counterparts.Industrial relevanceConsumers are increasingly aware of the link between food and health, maintaining a high demand for healthy products. In this regard, consumption of fatty fish, with healthy properties widely known, is lower than recommended by health authorities, especially in children. Novel processing technologies focused on increasing the appealing of products based on fatty fish can help in ameliorating this deficient consumption. Vacuum frying is a promising way of obtaining attractive products, due to some product modification after the deep-fat frying process, and yet retaining natural colour, juiciness and healthy properties to a high extent. Vacuum frying allows reducing ω3 fatty acids and tocopherol degradation, differentiating fish products so obtained, which can be launched into the market and benefit from this technology. Compared to conventional frying, the results are better nutritional and sensory properties in a final product.     

Water vapor transport properties during staling of bread crumb and crust as affected by heating rate

In order to develop a mathematical model to simulate mass transfer occurring between the crumb and the crust during bread staling, water vapor sorption properties, i.e., moisture diffusivity, WVP and sorption of bread crumb and crust were investigated at 15 °C. Two types of bread baked with two heating rates (7.39 °C/min and 6.32 °C/min) were considered. Sorption and desorption isotherms were determined using Dynamic Vapor Sorption (DVS) and FF and GAB models were applied in the range of 0–0.95 a_w, to fit isotherm curves. Diffusivity was determined from sorption isotherms by using Fick's law and WVP was measured by two methods (gravimetric and from sorption data). Results exhibited maximum values of D_eff in the range of 0.1 and 0.14 g/g d.b. moisture contents. They varied between 0.88 × 10^? 10 and 0.92 × 10^? 10 m²/s for the crust and between 2.24 × 10^? 10 and 2.64 × 10^? 10 m²/s for the crumb, baked respectively at 220 °C and 240 °C. Results of WVP showed that the crust baked at 240 °C was significantly more permeable than the crust baked at 220 °C. This fact was attributed to the difference in porosity and the molecular structure due to heating effects. Also, the presence of steam in the oven atmosphere enhanced the development of higher porosity in the crust, leading to different structures and properties. Moreover, SEM images showed that starch granules were intact and less swelled in the upper crust when baking at 240 °C, resulting in higher WVP.     

Proteolysis and storage stability of UHT milk produced in Turkey

The effect of raw milk quality (total and psychrotrophic bacterial and somatic cell counts, proteinase and plasmin activity) and UHT temperature (145 or 150 °C for 4 s) on proteolysis in UHT milk processed by a direct (steam-injection) system was investigated during storage at 25 °C for 180 d. High proteinase activity was measured in low-quality raw milk, which had high somatic cell count, bacterial count and plasmin activity. The levels of 12% trichloroacetic acid–soluble and pH 4.6-soluble nitrogen in all milk samples increased during storage, and samples produced from low-quality milk at the lower UHT temperature (145 °C) showed the highest values. Bitterness in UHT milk processed from low-quality milk at 145 °C increased during storage; gelation occurred in that milk after 150 d. The RP-HPLC profiles of pH 4.6-soluble fraction of the UHT milk samples produced at 150 °C showed quite small number of peaks after 180 d of storage. Sterilization at 150 °C extended the shelf-life of the UHT milk by reducing proteolysis, gelation and bitterness.     

Effects of ultrasound pretreatments on the kinetics of moisture loss and oil uptake during deep fat frying of sweet potato (Ipomea batatas)

The possibility of ultrasound assisted pretreatments of sweet potato to lower the moisture content and oil uptake during deep fat frying and its effects on the mass transfer rate was investigated. Sweet potato samples prior to frying were immersed in distilled water with ultrasound (UD), osmotic dehydration without ultrasound (OD) and ultrasound assisted osmotic dehydration (UOD). Ultrasound probe having frequency of 28 kHz at 300 W maximum power and time of 30 min was used for the pretreatment. The control (without pretreatment) and the pretreated samples were fried using sunflower oil at temperature of 130, 150 and 170 °C for 2, 4, 6, 8 and 10 min. The first order kinetic model was used for the mass transfer rate for moisture loss and oil uptake. The lowest moisture content was found in fried samples pretreated in UOD and OD while the lowest oil uptake was obtained in fried samples pretreated in UD having 65.11 and 71.47% oil reduction at temperature of 150 and 170 °C, respectively, compared to the untreated sample. The k values of all pretreated samples were higher than that of the untreated at 150 and 170 °C. The highest activation energy for moisture loss was found in untreated samples while the lowest activation energy for oil uptake was found in samples pretreated in UOD. The results from this work proved that ultrasound is a good pretreatment that can be used to obtain a low moisture content and oil uptake during deep fat frying of sweet potato.Industrial relevanceUltrasound is a novel technology that is widely used in the food industries because of its numerous advantages over conventional methods. Its application in the frying of foods could also help in the reduction of oil uptake, thus making safe the consumption of fried foods. The information about frying kinetics could also help in the design and optimization of the process in the food industry.     

Bread crispness and morphology can be controlled by proving conditions

Bread crust crispness is lost when water migrates from crumb to crust during storage. To what extent water migration is influenced by morphology is not known. Therefore, the effect of crispy rolls morphology on crust crispness was studied. Crispy rolls were prepared at three proving volumes: 300 mL (short proving), 500 mL (control) and 800 mL (long proving). X-ray microtomography was used to characterize morphology.Water transport from crumb to crust was determined. Short proved crispy rolls showed lower rate of crust water uptake while longer proved rolls showed faster uptake during cooling down. Sensory analysis revealed higher crispness for short proved crispy rolls after the same storage time at low RH. We hypothesize that shorter proved crispy rolls with finer crumb morphology, more closed structure, smaller gas cells with less gas cells interconnections and a thicker crust have a significant positive effect on water uptake kinetics and crispness retention.     

Effects of combined pretreatments on drying kinetics and quality of potato chips undergoing low-pressure superheated steam drying

Due to an increasing demand from health-conscious consumers more attention has been placed on investigating alternative techniques to replace conventional deep-fat frying to produce health-friendly snack products including potato chips. Recently, low-pressure superheated steam drying (LPSSD) has proved to have potential to produce fat-free potato chips if performed in combination with appropriate pre-drying treatments. In this study, the influences of various pretreatments and drying temperature on the LPSSD drying kinetics and quality parameters of dried potato chips were investigated. LPSSD of potato chips underwent various combined pretreatments, i.e., (a) blanching, (b) combined blanching and freezing, (c) blanching followed by immersion in glycerol solution and then freezing, and (d) combined blanching, immersion in monoglyceride solution and freezing, were carried out at different drying temperatures (70, 80, and 90 °C) at an absolute pressure of 7 kPa. The quality of the dried chips was then evaluated in terms of colors, texture (hardness, toughness and crispness) and microstructure. In terms of the drying behavior and the dried product quality, LPSSD at 90 °C with combined blanching and freezing pretreatments was proposed as the most favorable conditions for drying potato chips.     

Effect of processing conditions on the structure of monostearin–oil–water gels

The effect of processing conditions on the formation and stability of a gel comprised of oil, water, monostearin and stearic acid was studied. Processing conditions (homogenization rate (10,000–30,000 rpm), cooling rate (0.05–20 °C/min), homogenization temperature (70–95 °C)) had no effect on the initial storage modulus and melting temperature of the gel. Salt (NaCl) addition to the aqueous phase in the range 0–0.25% did not affect these parameters either; however, 0.5% salt addition lead to a ∼2 °C decrease in melting temperature and a doubling of the storage modulus. Optimal preparation conditions for the gel were mainly determined from its microstructure by polarized light microscopy – a smaller globule size and a more homogenous size distribution were considered a desirable feature. Optimum conditions included homogenization rates greater than 20,000 rpm, cooling rates above 6 °C/min, homogenization temperatures above, but close to, the Krafft temperature of monostearin (72 °C), and 0% salt concentration. The gelation temperature decreased by 2 °C with each 1 °C/min increase in cooling rate, from 1 °C/min to 5 °C/min.     

An investigation into lactose crystallization under high temperature conditions during spray drying

Lactose crystallization was studied at high temperature conditions in a Buchi B-290 mini spray dryer. The inlet gas temperature was 200 °C, and an insulating material was used to reduce the heat loss from the drying chamber (outlet temperature 157 °C), thus increasing the gas and particle temperatures. At these conditions, lactose crystallinity was found to increase significantly compared with a case where it was spray dried at 170 °C in a non-insulated drying chamber (outlet temperature 90 °C), but the process yield was lower for the former case (0.16% yield) than for the low temperature conditions (47% yield). There is some evidence that high-temperature spray drying of lactose is more likely to give more β-lactose anomer. Different analytical techniques (Fourier Transform Infrared Spectroscopy, modulated differential scanning calorimetry, moisture sorption test, Raman spectroscopy) were used to investigate the degree of crystallization and possible lactose anomer formation during this spray drying at high inlet gas temperatures.     

The effect of glaze uptake on storage quality of frozen shrimp

Ice-glazing is applied to protect the frozen shrimp from undesirable quality changes during frozen storage. Effects of initial frozen shrimp temperature on glaze uptake; glazing time on glaze uptake; and different glaze percentage on physical and chemical changes of frozen shrimp during storage were investigated. Shrimps were frozen in a spiral freezing machine (?35 °C/15 min); transferred to the air blast freezer until the core temperature reached ?18 °C, ?25 °C and ?30 °C; submitted to glazing process; and stored at ?18 °C for 180 days. The glazing percentage, pH and N-TVB levels were monitored every 45 days. This study has demonstrated the effectiveness of the glazing process as a protecting agent for frozen shrimp. A reasonable range of water uptake could be between 15% and 20% to guarantee the final quality. Therefore, it is important to prevent temperature fluctuations during transportation and storage to maintain the quality of the frozen shrimps.     

Thermal inactivation of Bacillus anthracis Sterne in irradiated ground beef heated in a water bath or cooked on commercial grills

The thermal stability of heat-shocked and non-heat-shocked spores of the virulence-attenuated Sterne strain of Bacillus anthracis was evaluated at select temperatures in irradiated, raw ground beef (25% fat) heated in a water bath or cooked using two different commercial grills. For the former, 3-g portions of inoculated ground beef were packaged in bags that were completely immersed in a temperature-controlled circulating water bath held at 65 °C (149 °F), 70 °C (158 °F), 75°(167 °F), and 80 °C (176 °F) for a predetermined length of time. For the latter, formed ground beef patties (95-g each) were inoculated with spore stock A or B of the Sterne strain and then cooked on a commercial open-flame gas grill or on a commercial clamshell electric grill to achieve target internal temperatures of either 71.1 °C (160 °F), 82.2 °C (180 °F), or 93.3 °C (200 °F). Cooking ground beef patties on commercial grills, resulted in reductions of ca. 0.8 to 3.5 log₁₀ CFU/g for spore stocks A and B of B. anthracis Sterne after heating to 71.1 °C (160 °F), 82.2 °C (180 °F), or 93.3 °C (200 °F) on either the open-flame gas grill which required ca. 9.6 min to reach the target internal temperatures or on the clamshell electric grill which required ca. 4.0 min to reach the target internal temperatures. In comparison, our data using a water bath system and heating at 65° to 80 °C predict nearly 4 log reductions in spore levels for short times, ~½ min, depending possibly on the temperature. Thus, our data suggest that models based on heating ground beef in a water bath is not a good predictor of reductions of levels of spores of B. anthracis Sterne strain that would be obtained when cooking ground beef patties on commercial grills under conditions that may be typically used by consumers and/or retail establishments. Nevertheless, our data validated that cooking ground beef patties on a commercial grill at a temperature considered to be “well-done” and a temperature (71.1 °C;160 °F) recommended by the USDA/FSIS, is effective at killing spores of B. anthracis Sterne.Industrial relevanceHeating ground beef in a water bath or cooking ground beef patties on commercial grills under conditions simulating those that are used by consumers and/or that occur in retail food service establishments is effective at killing spores of B. anthracis Sterne.     

Reduction of acrylamide by taurine in aqueous and potato chip model systems

Acrylamide in foods is mainly produced by Maillard reaction. Taurine can participate in the reaction, which has led us to investigate the possibility of reducing acrylamide formation by use of taurine. In an aqueous system, the lower the pH of the solution the greater the inhibition of acrylamide formation within a pH range of 5.0–8.0 was found, and the inhibition of acrylamide formation by taurine was dose-dependent. In a fried potato chip model, prior to frying at 170 °C for 3 min, the potato slices soaked in 0.l% to 2% taurine solution for 30 min showed significant reductions of acrylamide formation; however, these reductions were not dose-dependent. Also, the soaking treatments for 15–60 min significantly reduced acrylamide formation, but the inhibitory effects were not time-dependent. Thus, taurine, when used in a narrow range of reasonably low levels, is a candidate to inhibit acrylamide formation during frying process.     

Unsaturated fatty acid enriched vs. control milk triacylglycerols: Solid and liquid TAG phases examined by synchrotron radiation X-ray diffraction coupled with DSC

The fatty acid composition of milk triacylglycerols (TAGs) can be modified to improve their nutritional properties and the long-term health of consumers. However, the consequences of an increase in unsaturated fatty acid (UFA) content on the physical properties of milk TAGs remain to be elucidated. This study aimed at comparing the crystallisation properties and melting behaviour of control vs. UFA-enriched milk TAGs using the coupling of time-resolved synchrotron X-ray diffraction and differential scanning calorimetry on cooling and then heating at 3 °C/min. On cooling from the melt, high crystallisation temperature (HCT) TAGs solidify from 15.8 °C in α 2L (45–49 Å) structures, then low crystallisation temperature (LCT) TAGs form α 3L structures with a higher thickness (75.5 Å vs. 71.5 Å) and a delay (8.5 °C vs. 12.1 °C) for UFA-enriched TAG. On heating, melting of TAGs crystals and formation of 3L₂ (64.7–80.1 Å) and β′ 2L_f (40–44 Å) crystals associated with polymorphic reorganisations have been characterised. The organisation of TAG molecules in their liquid state revealed a higher thickness for UFA-enriched TAGs as compared to control TAGs. Such results improve the knowledge about the mechanisms of TAG crystallisation as a function of their FA composition and are important for the technological utilisation of UFA-enriched TAGs in food products.     

Cheese made from instant infusion pasteurized milk: Rennet coagulation,cheese composition,texture and ripening

Milk subjected to instant infusion pasteurization (IIP) at 72 °C, 100 °C and 120 °C (holding time 0.2 s) exhibited increased rennet coagulation time and decreased curd firming rate for increasing heat treatment temperature, when compared with raw or high temperature short time pasteurized (HTST) milk. However, addition of 4.5 mm or 9.0 mm of calcium restored the impaired rennet coagulation ability. Open texture cheeses produced from IIP milk (100 °C and 120 °C) contained significantly more moisture, had lower pH and shorter texture than similar cheese from IIP at 72 °C and HTST pasteurized milk. Cheese ripening was also affected by heat treatment, and different patterns of casein breakdown and peptide formation resulted from cheeses made from milk treated to IIP at 100 °C and 120 °C compared with cheeses made using IIP at 72 °C or HTST.