竹叶黄酮精华水乳液制备

基于竹叶黄酮具有防晒、保湿、美白等功效,在符合含量要求的范围之内,将竹叶提取物添加至水乳液中.首先对竹叶黄酮提取液进行了提取优化研究,并以芦丁作为标样,测定了竹叶提取液中黄酮含量。制备了竹叶黄酮精华水乳液并进行了乳液质感试用测评试验。结果表明:用20%(V/V)乙醇在25℃下超声提取20 min,竹叶黄酮提取效果较好。通过与市场上其它9个品牌的精华水乳液的质感试用测评,竹叶黄酮精华水乳液在使用质感上有较佳的表现,具有一定的市场前景。     

当归多糖和贡菊黄酮面膜的研究

目的:研究当归多糖和贡菊黄酮混合后的抗衰老和美白功效。方法:通过酪氨酸酶活性抑制实验和自由基清除实验,分别检测当归多糖、贡菊黄酮及当归多糖和贡菊黄酮混合后的自由基清除率和酪氨酸酶活性抑制率。结果:当归多糖对酪氨酸酶的抑制率为22.0%,贡菊黄酮对酪氨酸酶的抑制率为46.1%,当归多糖和贡菊黄酮混合物对酪氨酸酶的抑制率为66.2%,阳性对照组维生素E的酪氨酸酶的抑制率为88.7%。当归多糖对自由基的清除率为49.8%,贡菊黄酮对自由基的清除率为17.6%,当归多糖和贡菊黄酮混合物对自由基的清除率为46.5%,阳性对照组维生素E对自由基的清除率为65.1%。结论:实验结果表明当归多糖和贡菊黄酮混合物在抗衰老和美白方面具有一定的效果。     

天然功能性添加剂--竹叶提取物

竹叶中含有大量黄酮、酚酸、蒽醌类化合物、生物活性多糖、香豆素类内酯、特种氨基酸、芳香成分和锰、锌、硒等微量元素.竹叶提取物具有抗活性氧自由基、抗脂质过氧化、抗衰老、抗应激和抗疲劳、调节血脂、阻断亚硝化反应、增强免疫能力、抗菌、抑菌等生物学功能.国内目前只有安吉圣氏生物制品有限公司一家生产该类产品,年生产量为10t左右.     

中草药复方制剂治疗痤疮的后期效果

本文简述了粉刺的发病机理和中草药用于治疗粉刺的优点，重点对复方抗粉刺制剂“粉刺净”凝胶和“粉刺净”无纺布面膜的功效和安全性进行了临床测试，结果表明中草药复方制剂“粉刺净”凝胶和“粉刺净”无纺布面膜作为祛痘产品，不仅安全性较高，而且具有较好的长期效果疗效。     

从竹叶中同步提取多糖、茶多酚和黄酮的研究

研究高压脉冲电场法同步提取竹叶中黄酮、茶多酚和多糖的工艺。以竹叶为原料,在电场强度、脉冲数、提取介质pH和料液比4个单因素实验的基础上,采用L9(34)正交实验确定最优化工艺。结果表明,采用高压脉冲电场法进行同步提取,竹叶黄酮、茶多酚和多糖提取率分别可达到2.48%,0.93%和3.54%,竹叶黄酮与多糖的提取率比传统方法提高了25%以上,但茶多酚的提取率略有降低。该工艺可同步有效地从竹叶中提取黄酮、茶多酚和多糖,对于综合开发竹叶活性成分具有指导意义。     

灵芝发酵液的成分检测及美白与抗衰老功效评价

为开发灵芝发酵液在化妆品领域的应用,使用葡萄酒酵母发酵灵芝并测定发酵液中所含有的多糖、多酚、黄酮和多肽含量。通过DPPH自由基清除实验和羟自由基清除实验对灵芝发酵液的抗氧化能力进行检测,并测定其对成纤维细胞存活率的影响以评估其可能在抗衰老方面发挥的作用。最后通过体外酪氨酸酶实验和小鼠黑色素瘤细胞内的酪氨酸酶活性及黑色素合成抑制实验对灵芝发酵液的美白功效进行评价。结果表明,灵芝发酵液富含多糖,质量浓度为9.910 g/L,多酚、黄酮和多肽的质量浓度分别为0.054,0.045和2.931 g/L。体积分数分别为25.89%和42.18%的灵芝发酵液可清除50%的DPPH自由基和羟自由基;体积分数为0.05%~0.2%范围内的灵芝发酵液可有效促进成纤维细胞增殖;灵芝发酵液对酪氨酸酶活性抑制率高达73.22%,其通过抑制酪氨酸酶活性起到抑制黑色素合成的作用。因此灵芝发酵液具有一定的抗衰老和美白功效。     

响应面法优化超声辅助银杏叶中黄酮的提取及其抗自由基活性研究

目的 对银杏黄酮的提取工艺进行优化,并对其清除自由基活性进行研究;方法 通过响应面法优化乙醇浓度、提取温度、超声时间、液固比对产率的影响,体外清除自由基对抗氧化性进行研究;结果 当乙醇浓度为75%、超声时间控制为2 h、提取温度为80 ℃、液固比为30时,提取产率最高为2.82%,抗自由基结果表明,反应时间10 min清除率趋于稳定,银杏黄酮加入量1.00 mL,抗自由基活性高达68.9%; 结论 银杏黄酮具有较强的抗自由基活性,可以作为食品药品或者美容产品的添加剂.     

基于B16黑素瘤细胞评价体系的竹叶黄酮纳米粒子美白功效研究

以竹叶黄酮为载药、芫根酸性多糖为包材,以ε-聚赖氨酸为阳离子桥,采用离子交联法获得竹叶黄酮纳米粒子（BLFN）,平均粒径在400 nm左右,结构稳定,分散性良好。以业界公认的美白因子熊果苷为阳性对照,研究BLFN对B16小鼠黑素瘤细胞株体外培养过程中美白相关指标的影响。结果显示：BLFN对B16细胞内酪氨酸酶活性、黑色素合成和细胞增殖均表现出浓度依赖的抑制作用,当试样浓度100 μg/mL时,其抑制率分别达到69.68%、62.35%和59.14%,均显著优于熊果苷（p<0.05）。B16细胞的体外试验结果表明,BLFN能通过多种途径起到美白功效,显示出替代熊果苷的潜力。同时,由于该纳米粒子的构建成分均为天然物,具有较高的使用安全性,在美白类产品中的应用前景十分广阔。     

黄芩的生物活性及其在化妆品中的应用

论述了黄芩的资源、主要化学成分、提取方法、生物活性及其在化妆品中的应用等.黄芩中的主要活性成分黄芩苷和黄芩素等具有广泛的生物活性,如抗菌、抗真菌、抗炎、抗过敏、抗氧化、清除自由基、抗紫外线、抗辐射和抑制酪氨酸酶等.可制备防治痤疮、防晒、祛斑美白、抗皮肤老化和防皱等功效性化妆品,防治牙周炎等口腔用品以及外用洗剂等.黄芩素和黄芩苷等黄芩中主要活性成分是集祛斑美白、防晒、抗敏、消炎和抗衰老多效合一的天然高效多功能化妆品添加剂.     

美白面膜临床功效测试与分析

对自研美白面膜产品进行临床功效测试,评价其美白效果。选择32名有效健康志愿者作为研究对象,每隔2 d使用一次美白面膜,连续8周,同时停用其他美白护肤品或面膜。分别于使用前0周及使用后2,4和8周,测试志愿者面部含水量、黑红度、L~*值和b~*值,并拍摄VISIA的正面、左侧和右侧面部图片。分析结果显示,使用美白面膜,能够显著提高皮肤含水量,提高皮肤亮度L~*值和皮肤ITA°值,降低皮肤黑度和黄度b~*值,同时还能够降低皮肤毛孔、皱纹及纹理分值,即美白面膜具有良好的美白、使皮肤细腻、减少皮肤皱纹和纹理的功效。     

竹叶提取物的有效成分及其应用研究进展

综述了国内外竹叶提取物在实验和理论方面的研究进展。介绍了竹叶提取物的有效成分,包括黄酮类化合物、多糖、矿质元素和其他成分。论述了竹叶提取物有效成分的提取与分离技术。重点探讨了竹叶提取物的生物活性功能,如防腐、抗菌、抗氧化作用,以及竹叶提取物的植物源农药开发研究。对竹叶提取物的有效成分及其应用研究前景进行了展望。     

Cosolvent-modified supercritical carbon dioxide extraction of phenolic compounds from bamboo leaves (Sasa palmata)

This study highlights the possibility of supercritical carbon dioxide for extracting phenolic compounds from bamboo leaves that have shown antioxidant and anticancer activities. The CO₂ extraction solvent was modified by adding ethanol–water mixture cosolvent of different concentrations to allow extraction of both polar and non-polar compounds. Conventional Soxhlet extraction was also done to investigate the advantages of supercritical extraction over the conventional extraction method. For addition of 5% (mol) of a 25:75 (mol:mol) ethanol–water mixture solvent to CO₂, the highest amount of polyphenols (7.31 ± 0.06 mg/g bamboo leaves in catechin equivalents) and radical scavenging activity (3.65 ± 0.05 mg/g bamboo leaves in BHA equivalents) at 20 MPa and 95 °C, could be obtained among the mixture cosolvents studied. For Soxhlet extraction with a 25:75 (mol:mol) ethanol–water mixture, 1.48 times the amount of phenolic compounds (10.85 ± 0.52 mg/g bamboo leaves in catechin equivalents), could be isolated compared with the supercritical extraction method, however, the radical scavenging activity (3.30 ± 0.05 mg/g bamboo leaves in BHA equivalents) was 0.90 times lower than the extract obtained from the supercritical extraction method. The seven major antioxidative compounds identified from the SC-CO₂ extraction method were: (1) dl-alanine, (2) gluconic acid, (3) phosphoric acid, (4) ß-siosterol, (5) β-amyrene, (6) α-amyrin acetate and (7) friedelin.     

不同地区竹叶中多糖的提取测定

采用苯酚-硫酸法测定了10种竹叶多糖的含量,分析比较了不同竹种竹叶多糖含量的差异;利用离子色谱仪测定了竹叶多糖的组成。结果表明：10种竹叶含多糖0.18%~0.93%,最高为福建青秆竹,最低是长宁佛肚竹;同一地区不同竹种的竹叶多糖含量存在极显著差异。竹叶多糖由5种单糖组成,分别是阿拉伯糖、半乳糖、葡萄糖、木糖和甘露糖,其中阿拉伯糖占18.32%~36.63%,半乳糖占19.16%~38.19%,葡萄糖占16.90%~54.45%,木糖占5.88%~16.32%,甘露糖占1.53%~10.89%。     

竹叶黄酮护肤霜功效的研究

将竹叶黄酮添加到护肤霜基质中制得竹叶黄酮护肤霜,按有关标准对产品进行了性能测试和评价.护肤功效检测表明,含质量分数为1.5％的竹叶黄酮护肤霜在UVB区的平均吸光度为1.273,在UVA区的防晒效果达到三星级,具有较好的防晒功效;有优良的保湿功效;能显著降低皮肤黑色素含量而美白皮肤;可改善皮肤纹理度,降低皮肤粗糙度;增加皮肤弹性;且人体试验安全、无刺激性,是一种天然的化妆品功能性成分添加剂.     

Seasonal Variations of the Antioxidant Composition in Ground Bamboo Sasa argenteastriatus Leaves

Sasa argenteastriatus, with abundant active compounds and high antioxidant activity in leaves, is a new leafy bamboo grove suitable for exploitation. To utilize it more effectively and scientifically, we investigate the seasonal variations of antioxidant composition in its leaves and antioxidant activity. The leaves of Sasa argenteastriatus were collected on the 5th day of each month in three same-sized sample plots from May 2009 to May 2011. The total flavonoids (TF): phenolics (TP) and triterpenoid (TT) of bamboo leaves were extracted and the contents analyzed by UV-spectrophotometer. Our data showed that all exhibited variations with the changing seasons, with the highest levels appearing in November to March. Antioxidant activity was measured using DPPH and FRAP methods. The highest antioxidant activity appeared in December with the lowest in May. Correlation analyses demonstrated that TP and TF exhibited high correlation with bamboo antioxidant activity. Eight bamboo characteristic compounds (orientin, isoorientin, vitexin, homovitexin and p-coumaric acid, chlorogenic acid, caffeic acid, ferulic acid) were determined by RP-HPLC synchronously. We found that chlorogenic acid, isoorientin and vitexin are the main compounds in Sasa argenteastriatus leaves and the content of isovitexin and chlorogenic acid showed a similar seasonal variation with the TF, TP and TT. Our results suggested that the optimum season for harvesting Sasa argenteastriatus leaves is between autumn and winter.     

微波法提取竹叶中抗氧化物质的工艺探讨

以竹叶为原料,采用回流提取法、沸水浴提取法、超声波提取法、微波提取法提取其抗氧化性成分,通过测定吸光度利用Fenton反应比较对羟自由基的清除率,用正交实验对提取竹叶的工艺条件进行优化,结果表明:微波提取法效果较好;以60%的乙醇为提取剂,固液比为1g∶15mL,微波火力为60W,提取时间为20s时清除率最高。     

Allelopathic research of subtropical vegetation in Taiwan II. Comparative exclusion of understory byPhyllostachys edulis andCryptomeria japonica

On many hillsides of Taiwan there is a unique pattern of weed exclusion byPhyllostachys edulis (bamboo) andCryptomeria japonica (conifer) in which the density, diversity, and dominance of understory species are very different. Although the physical conditions of light, soil moisture, and soil nutrients strongly favor the growth of understory in a bamboo community, the biomass of its undergrowth is significantly low, indicating that physical competition among the understory species in the bamboo and conifer communities does not cause the observed differences. However, the biochemical inhibition revealed by these two plants appeared to be an important factor. The growth ofPellionia scabra seedlings, transplanted from the study site into greenhouse pots, was evidently suppressed by the aqueous leachate of bamboo leaves but was stimulated by that of conifer leaves. The radicle growth of lettuce, rye grass, and rice plants was also clearly inhibited by the leachate and aqueous extracts of bamboo leaves but not by those of conifer leaves. Six phytotoxins,o-hydroxyphenylacetic,p-hydroxybenzoic,p-coumaric, vanillic, ferulic, and syringic acids were found in the aqueous leachate and extracts of leaves and alcoholic soil extracts ofP. edulis, while the first three compounds were absent in the extracts ofC. japonica. The phytotoxicities of extracts were correlated with the phytotoxins present in both leaves and soils. The understory species might be variously tolerant to the allelopathic compounds produced by the two plants, resulting in a differential selection of species underneath. Therefore, comparative allelopathic effects ofPhyllostachys edulis andCryptomeria japonica may play significant roles in regulating the populations of the understories.Paper No. 253 of the Scientific Journal Series of the Institute of Botany, Academia Sinica, Taipei, Taiwan. This study was supported by the National Science Council of the Republic of China.     

赤水绵竹叶黄酮提取工艺方法研究

以芦丁为标准品,以贵州省赤水市绵竹叶为实验对象,对竹叶黄酮的提取进行研究,以乙醇浓度、反应温度、料液比、反应时间为主要考察因素,进行L9（3^4）正交实验,对竹叶黄酮提取条件进行系统研究,确定黄酮最佳提取工艺为乙醇提取浓度75％,反应温度70℃,料液比1：20,反应时间5．5h。     

竹叶中绿色素的稳定性研究

研究了用食用乙醇浸提竹叶制得的绿色素的稳定性。结果表明,该色素在碱性条件下对热、阳光和紫外光均具有良好的稳定性。