Multiple tarsal coalitions in the same foot

I reviewed 60 cases of tarsal coalition seen at Texas Scottish Rite Hospital for Children over the last 10 years. Twenty-nine patients had plain films only and were excluded from the study. One patient was diagnosed with synovitis at the time of surgery and was also excluded. Thirty patients with 53 coalitions had computed tomographic (CT) and, in many cases, surgical documentation of a tarsal coalition. Of these 30 patients, six had multiple coalitions in the same foot. This article briefly summarizes the six cases and reviews the literature of multiple tarsal coalitions, both before and after the use of CT. I suggest that multiple coalitions in the same foot are more common than once thought and recommend CT evaluation of both feet in transaxial and coronal planes in patients with suspected tarsal coalition.     

Repetitive axonal stimulation of the same single motor unit: a longitudinal tracking study

We elicited three single motor unit action potentials (S-MUAPs) via multiple point stimulation and subjected them to repetitive stimulation (RS) in 3 healthy subjects. We tracked each S-MUAP and its RS trains over two separate sessions as well as the compound motor action potential (CMAP) RS trains obtained from the same muscle following whole nerve stimulation. Repetitive axonal stimulation yields consistent results when carefully performed with minimal variation in each S-MUAP RS train from session to session, providing previously unobtainable data regarding neuromuscular junction function in the same single motor unit over time. In this small, normative sample, no significant correlation between S-MUAP and CMAP RS responses was observed.     

Storage telemetric system for detection of radionuclide tracers in humans

The action of a psychotropic substance, N-ethyl-2(2-methoxy-5--sulfamido-benzamidomethyl)-pyrrolidine (sulpiride, Dobren), on cellular metabolism has been studied by means of the in vitro lymphocyte activation technique. The effect of the continuous presence of the compound in the culture, at a concentration permitting cellular vitality was evaluated. Furthermore the effect of the drug on the culture during the lymphocyte stimulating phase and during the incorporation phase of 3H-thymidine was separately evaluated. The results indicate that the sample population can be divided into two different groups depending on the modality of the response to the drug in vitro taken as activity index (A.I.). Whether our conclusion has any practical significance remains to be elucidated.     

The cortico-related zones of the rabbit claustrum-study of the claustrocortical connections based on the retrograde axonal transport of fluorescent tracers

The claustrocortical connections in the rabbit were assessed for the first time by the method of axonal retrograde transport of two fluorescent tracers (Fast Blue and Diamidino Yellow). The material consisted of 23 adult New Zealand rabbits. Projection zones of spindle-like form, connected with the precentral, postcentral, temporal and occipital cortices have been delineated. They are organized topographically both in the anteroposterior and ventrodorsal direction. The precentral (motor) projection zone is localized in the anterodorsal part of the claustrum. It may be divided into two separate parts that project to the medial and lateral part of the precentral cortex. The large postcentral (somatosensory) zone occupies mainly the central part, whereas the temporal (auditory) and occipital (visual) zones are situated in the posteroventral part of the claustrum. The overlap of various claustral projection zones is differentiated, the largest being that of the somatosensory zones. In comparison to the results of study of claustral projection zones performed on other species, presumably on the rat and cat, its seems plausible to conclude that the extension of claustral projection zones and degree of their overlap in the rabbit represent an intermediate character.     

Median eminence-afferent vasoactive intestinal peptide (VIP) neurons in the hypothalamus: localization by simultaneous tract tracing and immunocytochemistry

Retrograde tract tracing and immunocytochemistry were used to investigate the CNS source of the VIP that is present in high concentrations in the hypophysial portal blood and has been shown to have a stimulatory effect on pituitary prolactin secretion. Fluoro-gold (FG), which enters the CNS through areas devoid of the blood-brain barrier, such as median eminence, was injected peripherally. Brain sections from FG-treated animals were immunostained for VIP. A small population of VIP-containing cell bodies in the parvocellular and periventricular parts of the paraventricular nucleus (PVN) was also labeled with FG. Vasoactive intestinal peptide-immunoreactive perikarya not labeled with FG were also observed in the PVN, as well as FG-labeled cells that did not contain VIP. The results suggest that some VIP-producing neurons in the PVN project to the median eminence and are, therefore, functionally related to pituitary regulation; the function of other VIP neurons in the PVN is unknown.     

Eukaryotic lipid-biosynthetic enzymes: the same but not the same

The discovery that different eukaryotic enzymes catalyse the same lipid-biosynthetic reaction has paralleled the recognition that segregated pools of lipids have unique biological functions. This review considers enzymes involved in the synthesis of diverse classes of lipids--glycerolipid precursors, phospholipids, sterols and eicosanoids--and summarizes recent data that show that these duplicate enzymes are frequently encoded by different genes and have unique subcellular locations. Does this duality merely represent redundancy or do the different isoforms provide pools of lipids for specific biological purposes?     

Analysis of the total activation cross section of all possible reactions producing the same radioactive nuclide for the

Firstly, according to the regulation of growth and decay of radioactive nuclides produced in reactions, a formula used to calculate the total activation cross section of all possible reactions producing the same radioactive nuclide for the same element is     

The Efe forager infant and toddler's pattern of social relationships: Multiple and simultaneous.

Extends previous observations on the multiple caretaking system of newborns and young infants among the Efe foragers of Zaire, to Efe infants and toddlers, whose social contacts with mother, father, adults, and children at 5 and 8 mo and at 1, 2, and 3 yrs were observed naturalistically. The Efe infant experiences a pattern of simultaneous and multiple relationships rather than a pattern that is initially focused on 1 person, most typically the mother, and that with development progresses to other relationships. This pattern is influenced by physical and social ecological factors and cultural practices. It is argued that (1) this pattern of social experience leads to a sense of self that incorporates other people and (2) some assumptions about the nature of social development, especially that early relationships are hierarchical and sequential in nature, require reevaluation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Multiple eruptive dermatofibromas in three men with HIV infection

Several recent studies have investigated the effects of growth hormone (GH) on the healing of fractures and bone ingrowth, but with conflicting results. The negative results may be due to antibody formation against injected GH or because some experimental models are able to prove only positive GH effects. In this study, we wanted to investigate the effect of GH on implant integration in bone. To avoid potential formation of antibodies against injected GH, we used a model with transgenic mice overexpressing bovine GH (bGH). Titanium implants were inserted in the forehead of the mice. 4 months after insertion, the implants were cut out en bloc with the surrounding bone. The calcified specimens were cut and ground to a thickness of approximately 10 microns. Histomorphometry demonstrated significantly more direct bone-to-metal contact in the transgenic mice than in the nontransgenic littermates. Our findings indicate that systemic administration of GH in humans may improve implant integration in bone.     

Analytical method development for the simultaneous quantitation of dexmedetomidine and three potential metabolites in plasma

Dexmedetomidine, a novel alpha 2-adrenergic receptor agonist, is being developed as an anesthetic adjunct for perioperative use. An assay method has been developed for the sensitive quantitation of dexmedetomidine and three metabolites in plasma: MPV-1305, MPV-1306 and MPV-1709. The method involves solid-phase extraction (C18 cartridge) of dexmedetomidine and metabolites followed by a two-step derivatization. The first step utilized BF3-MeOH to simultaneously mask a primary alcohol in MPV-1305 and a carboxylic acid in MPV-1306. The second step applied PFB-Cl to derivatize the imidazole ring for sensitive detection of these compounds by GC-negative chemical ionization MS at pg/ml concentrations. MPV-1709 was not derivatized in the process and was detected by GC-positive chemical ionization MS. Optimization of extraction and derivatization is discussed. The method is suitable for quantitation of dexmedetomidine, MPV-1305, MPV-1306 and MPV-1709 over concentration ranges of 0.1, 40, 0.5-100, 0.5-100 and 1.0-500 ng/ml, respectively. The method showed excellent specificity, linearity and sensitivity and is useful for profiling the pharmacokinetic disposition of these compounds.     

An array immunosensor for simultaneous detection of clinical analytes

A fluorescence-based immunosensor has been developed for simultaneous analysis of multiple samples. A patterned array of recognition elements immobilized on the surface of a planar waveguide is used to "capture" analyte present in samples; bound analyte is then quantified by means of fluorescent detector molecules. Upon excitation of the fluorescent label by a small diode laser, a CCD camera detects the pattern of fluorescent antigen:antibody complexes on the sensor surface. Image analysis software correlates the position of fluorescent signals with the identity of the analyte. This immunosensor was used to detect physiologically relevant concentrations of staphylococcal enterotoxin B (SEB), F1 antigen from Yersinia pestis, and D-dimer, a marker of sepsis and thrombotic disorders, in spiked clinical samples.     

Rapid detection of the Chlamydiaceae and other families in the order Chlamydiales: three PCR tests

Few identification methods will rapidly or specifically detect all bacteria in the order Chlamydiales, family Chlamydiaceae. In this study, three PCR tests based on sequence data from over 48 chlamydial strains were developed for identification of these bacteria. Two tests exclusively recognized the Chlamydiaceae: a multiplex test targeting the ompA gene and the rRNA intergenic spacer and a TaqMan test targeting the 23S ribosomal DNA. The multiplex test was able to detect as few as 200 inclusion-forming units (IFU), while the TaqMan test could detect 2 IFU. The amplicons produced in these tests ranged from 132 to 320 bp in length. The third test, targeting the 23S rRNA gene, produced a 600-bp amplicon from strains belonging to several families in the order Chlamydiales. Direct sequence analysis of this amplicon has facilitated the identification of new chlamydial strains. These three tests permit ready identification of chlamydiae for diagnostic and epidemiologic study. The specificity of these tests indicates that they might also be used to identify chlamydiae without culture or isolation.     

Comparison of automatic boundary detection and manual tracing technique in echocardiographic determination of left atrial volume

Previous reports have indicated that echocardiography with automatic boundary detection (ABD) is useful for the noninvasive estimation of left ventricular volume. However, few data exist regarding the measurement of left atrial (LA) volume, which also provides pivotal information in the clinical setting. Therefore, the feasibility of LA volume measurement by ABD in comparison with the manual tracing using modified Simpson's method (SM) was evaluated. Fifty-nine patients with coronary artery-disease with sinus rhythm were examined. Using ABD, a region of interest was set around the LA border and mitral annulus from an apical four-chamber view. The maximal and minimal LA volume (Vmax and Vmin) were measured from the volume waveform. Using the SM, the maximal and minimal LA volume were measured by the manual tracing on frozen frames at the apical four-chamber view. The ABD displayed a curve of LA volume change that consisted of passive emptying, diastasis, and active emptying phases during the left ventricular diastolic period. Under these conditions, the Vmax and Vmin were 43.7 +/- 11.2 ml and 21.1 +/- 7.6 ml, respectively, yielding the volume change of 22.6 +/- 6.0 ml. By the SM, Vmax and Vmin were 43.1 +/- 9.9 ml (r = 0.94, p < 0.0001, y(ABD) = 0.91x (SM) + 3.6) and 22.0 +/- 9.0 ml (r = 0.91, p < 0.0001, y = 0.94x + 0.7), respectively, and the volume change was 22.8 +/- 6.1 ml (r = 0.82, p < 0.0001, y = 0.84x + 3.8). These results indicate that the ABD from the apical four-chamber approach could provide an accurate estimation of LA volume change, suggesting the potential value of this method in assessing LA function, although some technical difficulties need to be further overcome.     

Posttraumatic hypothermia in the treatment of axonal damage in an animal model of traumatic axonal injury

Twenty-one-day-old BALB/c mice were shaved on the back to synchronize hair growth. On day 30 or 31, when at least 90% of mice exhibited hair regrowth in the shaved area, 1,25(OH)2D3 was applied topically to the shaved area daily for 5 days. On the 6th day, cyclophosphamide (Cytoxan, CTX) was injected i.p. to induce hair loss in the shaved area. Alopecia was induced in a dose-dependent manner by CTX treatment within 1 to 2 weeks. This effect was reduced significantly if mice were pre-treated with 1,25(OH)2D3, though only slight protection was observed in female mice. Interestingly, this 1,25(OH)2D3-mediated protection against hair loss was attenuated in male mice but became more significant in female mice when they were inoculated with the EMT-6 murine mammary tumor prior to treatment. More importantly, topical treatment with 1,25(OH)2D3 alone was able to inhibit EMT-6 tumor growth in both male and female BALB/c mice. Furthermore, 1,25(OH)2D3 pre-treatment also augmented the anti-tumor effect of CTX. Our results demonstrate that topical application of 1,25(OH)2D3 can protect against CTX-induced alopecia both in tumor-free and in tumor-bearing mice in a sex-dependent manner. Moreover, 1,25(OH)2D3 was shown, either alone or in combination with CTX, to inhibit tumor growth.     

Multiple endocrine neoplasia-type medullary thyroid carcinoma in three generations of a family

The familial accumulation of a multiple endocrine neoplasia (MEN) type 2a medullary thyroid carcinoma, is described based on the retrospective analysis of a family history. The proband was characterized by medullary carcinoma (MC) combined with phaeochromocytoma, her child had been shown to suffer from MC. In the third generation of the family C-cell hyperplasia and bilateral adrenal hyperplasia occurred. It is shown that in order to arrive at a correct decision as regards the therapy to be used, modern laboratory tests (serum calcitonin, CEA-analysis) and diagnostic imaging methods (ultrasound, computer tomography, magnetic resonance imaging, positron emission tomography, metaiodobenzylguanidine scintigraphy) should be used. It is emphasized that the available therapeutic means (surgery, radiotherapy, nuclear medicine) have to be carefully selected and, if necessary, combined. In medullary thyroid carcinoma associated tumours in other endocrine organs should be expected to occur. Family screening using blood chemical and genetic tests are recommended in asymptomatic cases, since their surgical treatment can in this way lead to complete recovery.     

炼钢–连铸区段3种典型工序界面技术研究进展

面对钢厂智能化发展的时代要求,炼钢–连铸区段工序界面技术受到越来越多冶金学者的关注,其不仅是解决工序关系集合协同–优化问题的重要手段,也影响着工序功能集合解析–优化和流程工序集合重构–优化的效果。本文对炼钢–连铸区段3种典型工序界面技术,即钢包运行控制、天车运行控制和生产运行模式优化的研究进展进行阐述,其中,钢包运行控制包括钢包热状态监测、钢包选配以及钢包调度,天车运行控制包括吊运任务的分配和同跨/异跨天车的协同调度,生产运行模式优化包括工序/设备产能、时间节奏与炉–机对应模式的匹配设计。此外,针对炼钢–连铸区段多工序协同运行的制约因素,指出工序界面技术协同的必要性,并对上述工序界面技术的协同机制与协同方案进行了阐述。