谷物蛋白分离分析方法

介绍了目前分离分析谷物蛋白的最新方法。用ＡＵＣ缓冲液和排阻色谱（ＧＰ－ＨＰＬＣ）能有效分离米糠蛋白;用毛细管电泳和碱性硼酸钠缓冲液分离小麦蛋白,可得到４０多个醇溶蛋白组分和２０多个低分子量谷蛋白亚基（ＬＭＷ－ＧＳ）,以相可鉴别品种差异。     

小麦醇溶蛋白和谷蛋白亚基的高效毛细管电泳分离研究

初步探讨了小麦醇溶蛋白和低分子量谷蛋白亚基（ＬＭＷ－ＧＳ）高效毛细管电泳（ＨＰＣＥ）分离的分辨芳及其重复性。结果显示：利用碱性硼酸钠缓冲液系统及一个改进的毛细管冲洗程序分离麦醇溶蛋白，可获得较高的分辨度和重要性。通常在３０ｍｉｎ内一个品种较易分离出４０个左右醇溶蛋白组分，而且重复分离之间峰迁移时间和峰高的平均相对标准偏差（ＲＳＤ）可分别保持在０．２％和４％左右。利用ｐＨ２．５酸性磷酸缓冲液（含２０     

文摘

文摘以牛奶为基础的酸性食用凝胶的生产方法（ＢＵ－ＤＯＬＦＳＥＮ，Ｇ；ＮＩＥＣＳＥＮ，Ｐ．Ｍ）ＰＣＴＩｎｔｅｒｎａｔｉｏｎａｌＰａｔｅｎｔＡｐｐｌｉｃａｔｉｏｎ（１９９４）ＷＯ９４／２１１２９ＡＩ，７ｐｐ［英文］ＤＳＡ．Ｐ１０７（１９９５）８０８文章报...     

现代分离分析技术与银杏叶提取物中生物活性成分的研究

重点介绍超临界流体萃取和色谱（ＳＦＥ和ＳＦＣ）、高效液相色谱（ＨＰＬＣ）、高效毛细管电泳（ＨＰＣＥ）、核磁共振（ＮＭＲ）、热喷雾液相色谱－质谱（ＴＳＰ－ＬＣ－ＭＳ）等联用技术在银杏叶提取物（ＧＢＥ）中生物活性成分的分离、纯化和结构鉴定等应用领域的研究进展。     

大豆原料及其分离蛋白的SDS-PAGE图谱研究

将大豆原料制备成脱脂豆粕、“碱提酸沉”分离蛋白和超滤法分离蛋白,采用ＳＤＳ－ＰＡＧＥ技术研究其中的电泳图谱变化。在所有样品的ＳＤＳ－ＰＡＧＥ图谱中共分离出１７种蛋白组分。其分子量（Ｍｒ）分别是：１号带,２２８４０（２,３）号带,２６０２０：４号带,３００８０;５号带,３２８２０：（６、７）号带,３６８５０：８号带,４６５００：９号带,５１４３０：１０号带,５６９２０：１１号带,６５７９０：１２号带     

ITMA‘95展出起毛,剪毛,磨毛机械的介绍

ＩＴＭＡ’９５展出起毛、剪毛、磨毛机械的介绍郑绥新（海宁纺织机械实业总公司）本次展览会共有十六家起毛、剪毛、磨毛厂商参展。其中有意大利的ＬＡＦＥＲ、ＣＯＭＥＴ、ＣＡＲＵ、ＬＡＭＰＥＲＴＩ、ＲＩＭＡＲ、ＰＡＯＬＯ、ＣＲＯＳＴＡ、德国的ＨＥＲＧＥＲＴ、Ｓ...     

第八届北京木工机械展主要厂商及展品简介

公司名称产品及业务（续） ＩＴＡＬＹ意大利Ａ ＣＯＳＴＡ Ｓ．Ｐ．Ａ．封边机、横切机、木地板生 产线ＡＬＦＡＭＡＣＣＨＩＮＥ Ｄ ＲＡＦ－框架装配机、双横切锯ＦＯＮＩ ＧＩＵＳＥＰＰＥＡＮＧＥＬＯ ＣＲＥＭＯＮＡ原木制备设备Ｓ．Ｐ．Ａ．ＡＲＴＵＲＯ ＳＡＬＩＣＥ Ｓ．Ｐ、Ａ．家俱自动关闭铰链ＡＵＴＥＣＯ Ｓ．Ｒ．Ｌ．多轴四面铣刨机ＢＡＬＥＳＴＲＩＮＩ ＲＥＮＺＯ实木餐椅生产机械Ｓ．Ｐ．Ａ．ＢＩＥＳＳＥ ＧＲＯＵＰ木工机械ＢＭＡ ＡＢＲＡＳＩＶＥＳ砂纸ＢＲＥＶＥＴＴＩＭＡ双锯斜角切割机、固定、涂 胶、基砖材料接…     

乌骨鸡,罗曼鸡卵黄蛋白质电泳分析

本文通过ＩＥＦ－ＰＡＧＥ、ＳＤＳ－ＰＡＧＥ、ＰＡＧＥ对乌骨鸡、罗曼鸡卵黄蛋白质组成，、比例、等电点以及水溶性各种蛋一进行了初步研究。在ＩＥＦ－ＰＡＧＥ中，乌骨鸡卵黄蛋白分出２５条蛋白区带，罗曼鸡卵黄蛋白分出２３条蛋区带，ＳＤＳ－ＰＡＧＥ、ＰＡＧＥ中罗鸡水溶性蛋白均分出１４条蛋白区带，乌骨鸡水溶性蛋白均分出１１条蛋白区带。     

顶青霉木聚糖酶的分离纯化与鉴定

使用硫酸铵分级沉淀、ＳｅｐｈａｄｅｘＧ－２５凝胶色谱脱盐、ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－２５和ＣＭ－ＳｅｐｈａｄｅｘＣ－５０离子交换色谱等分离纯化技术,从顶青霉（Ｐｅｎｃｉｌｉｕｍｃｏｒｙｌｏｐｈｉｌｕｍ）培养液中分离到３种木聚糖酶组分,分别称为ＰａｒｔＡ、ＰａｒｔＢ和ＰａｒｔＣ．ＰａｒｔＢ和ＰａｒｔＣ经ＳｅｐｈａｃｒｙｌＳ－２００凝胶过滤色谱和ＣｏｎＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和色谱进一步分离纯化,分别得到２个纯木聚糖酶组分．ＰａｒｔＢ经ＳＤＳ－ＰＡＧＥ鉴定为单带,相对分子质量为２４２００;ＰａｒｔＣ经ＳＤＳ－ＰＡＧＥ鉴定基本为单带,相对分子质量为４８３００     

L－缬氨酸菌种选育

以硫酸二乙酯（ＤＥＳ）诱变处理黄色短杆菌（Ｂｒｅｖｉｂａｃｔｅｒｉｕｍｆｌａｖｕｍ）ＸＱ５１２２，得到突变株Ｖ３－３６＜Ｌｅｕ￣１、α－ＡＢ￣ｒ、ＡＨＶ￣ｒ），在１０％葡萄糖培养基中可积累２．３％Ｌ－缬氨酸。以亚硝基胍（ＮＴＧ）诱变Ｖ４－１５３，得到一株突变株（Ｌｅｕ￣１、α－ＡＢ￣ｒ、ＡＨＶ￣ｒ、２－ＴＡ￣ｒ），再进行单菌落分离，得到突变株ＺＱ－２，能在培养基中积累Ｌ－缬氨酸４．２％～４．５％，最高达５．５７％．     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press