共查询到20条相似文献,搜索用时 15 毫秒
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The reactivity of the antibovine monoclonal antibodies (mAbs) comprising temporary cluster TC1 was compared with that of two OvCD45R mAbs on sheep cells. Three of the mAbs--CC31, CC99 and CC103--did not cross-react with sheep cells. All the workshop mAbs precipitated two molecules of apparent molecular weight (MW) 200 kDa and 220 kDa while the antisheep CD45R mAb 20-96 precipitated a single band of 220 kDa. Cell surface expression was examined by single colour FACS (fluorescence activated cell sorting) analysis of efferent and afferent lymph cells and peripheral blood lymphocytes and the distribution of the antigens on CD4+, CD8+ and T19+ (WC1) and B cells was determined by two colour fluorescence staining. By cellular distribution and immunohistology the TC1 mAbs could be divided into four distinct groups which differed from a fifth group comprising the two OvCD45R antibodies. 相似文献
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The high yield strength and elastic modulus of metallic glasses suggests they could perform an important role in structural applications. To produce materials with a high strength-to-weight ratio and excellent mechanical energy absorption, it is advantageous to form amorphous alloys as cellular solids. Using the elastic properties of slip cast amorphous Fe45Ni45Mo7B3 ribbons, a metallic glass honeycomb was manufactured with a unique manufacturing approach. First, prototypes were manufactured with a porosity of 97?pct, a cell wall thickness of 0.03?mm, and a cell size of 3?mm. Experimentally measured mechanical properties were reasonably similar to analytical models. This suggests that a three-times improvement in the yield strength along the out-of-plane direction is achievable when compared with crystalline aluminum honeycombs. An analytical model was developed to predict the relative density and the compressive stress (?? 3 * ) in the out-of-plane (X 3) direction of the ??teardrop?? cellular structure. The predictions are validated by initial experimental results and compare well with existing analytical models for hexagonal cellular materials. 相似文献
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FA Zuckermann C Peavey WM Schnitzlein D Schabacker RJ Husmann H Yang A Saalmüller JK Lunney 《Canadian Metallurgical Quarterly》1998,60(3-4):367-387
Swine cell binding analyses of a set of 48 monoclonal antibodies (mAbs), including eleven standards, assigned to the CD44 and CD45 subset group of the Second International Swine CD Workshop yielded 13 clusters. Although none of these corresponded to CD44, seven mAbs formed a cluster which was identified as being specific for restricted epitopes of CD45 (CD45R). In addition, a T-cell subset specific cluster comprised of four mAbs was also identified. Two mAbs (STH106 and SwNL 554.1) reacted exclusively with CD8 bright lymphocytes, the other two (2B11 and F01G9) with a subset of CD4 lymphocytes. The other 10 clusters were either specific for MHC-class I like molecules or overlapped with clusters identified by the adhesion molecule subgroup and are therefore just briefly discussed in this report. The specificity of all the mAbs in the CD45R cluster was verified by their ability to immunoprecipitate distinct proteins and to react with CHO cells expressing individual isoforms of CD45. Three CD45R mAbs (3a56, MIL5, -a2) did react with a 210 kDa isoform(s), while another three (STH267, FG2F9, 6E3/7) only recognized a 226 kDa isoform(s). The remaining one (MAC326) precipitated both a 210 and 226 kDa protein. The specificity of all the mAbs in the CD45R cluster, and of the CD45 common mAbs, was confirmed by their reactivity with CHO cells transfected with cDNAs encoding the extracellular and transmembrane portions of distinct CD45R isoforms. Those mAbs recognizing a 210 kDa protein reacted with CHO cells expressing the CD45RC isoform, while those capable of precipitating a 226 kDa, but not the 210 kDa, polypeptide recognized CHO cells expressing either the CD45RAC and the relatively rare CD45RA isoform. MAC326 was unique in its inability to react with CHO cells engineered to produce the CD45RC and CD45RAC isoforms. Thus, three mAbs (6E3/7, STH267, and FG2F9) appear to be specific for an epitope(s) encoded by the A exon, while one (MAC326) recognizes a determinant encoded by the C exon. The remaining three mAbs (3a56, -a2, MIL5) are apparently specific for an epitope(s) which results from the fusion of the C exon to the invariant leader sequence and is destroyed by inclusion of the A exon. All three CD45 common mAbs, K252.1E4, MAC323 and 74.9.3, did react with the CHO cells lines expressing either the CD45RA, CD45RC, CD45RAC or CD45RO isoforms, but not with untransfected CHO cells. When the natural expression of CD45 isoforms was examined by reacting lymphocytes with CD45R mAbs, a high level expression of isoforms containing the A exon-generated domain was detected in all B cells while the majority of CD4+ T cells had undetectable or lower expression density of this protein than B cells. In contrast, the density of expression of the CD45 isoform(s) containing the C exon-generated domain ranged from undetectable to high in CD4+ T cells whereas the amounts were approximately ten-fold lower in B cells. Overall this panel of CD45 mAbs will be very useful in analyzing the maturation and differentiation of swine lymphoid cells subsets. 相似文献
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韶钢炼轧厂生产的 45号钢在常规生产检验中发现材料规格对力学性能初检合格率有影响 .为此 ,随机抽取16、18、2 8、3 0mm 4个规格、11个炉号的 45号钢进行正火工艺试验 ,探讨了正火工艺对 45号钢力学性能的影响 .结果表明 :直径小于 2 5mm的 45号钢 ,正火工艺采用GB/T699- 1999标准推荐的工艺执行 ,即 85 0℃×40min ,力学性能达到标准要求 ;直径大于或等于 2 5mm的 45号钢 ,正火工艺采用 880℃× 3 0~ 60min也可达标 .金相检验表明 :这两种工艺处理试样的金相组织均为块状铁素体和大小均匀的珠光体 ,这种组织能够满足强度和塑性的理想配合 相似文献
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K Nishikawa T Miyasaki N Tsukaguchi Y Noma K Nakagawa N Narita 《Canadian Metallurgical Quarterly》1996,44(6):548-553
Flow cytometry is a powerful tool for immunophenotyping of acute leukemia. Gating of leukemic cells is currently performed on the two dimensional display of forward scatter (FSC) and side scatter (SSC). However, this gating method can not discriminate leukemic cells from contaminated normal cells. Therefore, we used a CD45 monoclonal antibody to detect leukemic cells (CD45dlm cells) in combination with the SSC parameter. This CD45-SSC gating method was very useful for immunotyping of AML and ALL, especially leukemia with a low percentage of blasts. We recommend this new gating method for more accurate immunophenotyping of acute leukemia. 相似文献
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Forty-five patients with Williams syndrome (WS) were evaluated for oral abnormalities. The mean age of the patients was 9.25 years, the median age was 6.7 years, and the majority (62.2%) were male. Hypodontia was present in 11.1% of the patients. Abnormal tooth morphology was noted in 12.5% of the primary dentitions and 40.7% of the permanent dentitions. With the exception of the primary mandibular central incisors of males, all mesiodistal incisor crown dimensions were statistically significantly smaller when compared with norms (P < 0.05). At least one hypoplastic enamel defect was present in 9.4% of patients with primary teeth and in 18.5% with permanent teeth. No patients exhibited generalized enamel hypoplasia. More than half of the patients (59.1%) were both caries and restoration free, while only 13.6% presented with clinically active caries. Tongue thrusting was present in 67.7% of the sample, while more than 50% of the patients present with excessive interdental spacing. Patients exhibited a higher than normal prevalence of Class II and III occlusions, open and deep bites and anterior crossbites. No single dental finding was pathognomonic of WS, however two constellations of findings, each occurring in approximately one-third of the sample, were observed: 1) microdontia, anterior crossbite, tongue thrusting, and excessive interdental spacing, and 2) microdontia, deep or open bite, and excessive interdental spacing. 相似文献
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The flow in a junction manhole is analyzed using hydraulic modeling and a semiempirical approach. A large number of experiments was conducted to obtain insight in the complex hydraulic features of flows in either one or both of the approach junction branches. Given their significance in applications, in addition to supercritical flow conditions, junction flow with mixed approach flows were also considered. The results include information regarding the main wave structure, with detailed discussions of locations and heights of so-called waves A, B, and C. Further, the swell height at the downstream manhole end was determined and it was found that choking of the supercritical flow structure occurs mainly because of a capacity limit at this outlet. Then, both the minimum and maximum flow limitations were determined. If the discharge is smaller than the minimum discharge, there is a transition from supercritical to subcritical junction flow. If, on the other hand, the discharge is larger than the maximum discharge, supercritical junction flow breaks down and a pressurized two-phase flow is established, which can be responsible for dangerous phenomena such as manhole geysering. 相似文献
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研究了大规格 45V非调质钢加热工艺、终轧温度和冷却速度等轧制工艺对综合力学性能的影响。并研究了获得良好综合力学性能而优化的轧制工艺 相似文献
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介绍了安钢第二炼钢厂45号钢的生产工艺,确定了45号钢工艺参数及控制措施。 相似文献
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针对传统AOD配气方式的缺点,研究了基于魏季和等的脱碳精炼数学模型的配气模型,使AOD脱碳配气可根据钢水碳含量的变化自动调整氧氩比、根据钢水温度变化自动调整供氧强度,以及根据炉役中炉容比的变化自动调整最大供氧强度。该模型应用于AOD不锈钢冶炼系统中,既提高了氧气利用率,又缩短了冶炼时间,而且吨钢氧耗和还原硅消耗也略有改善,取得了较好的效果,为AOD不锈钢冶炼智能化打下了良好的基础。 相似文献
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F Górski 《Canadian Metallurgical Quarterly》1976,29(13):1169-1171