Evaluation of total phenol content and antioxidant properties of encapsulated grape seed extract in yoghurt

The effect of encapsulated (En) and nonencapsulated (NE) grape seed extract (GSE) at 1% level added to milk before its fermentation was evaluated on the physicochemical properties, total phenolic content and antioxidant activity of the resulting yoghurt. Encapsulated GSE resulted in a product comparable to the control, based on sensory properties, acidity, water‐holding capacity, viscosity and colour, along with a threefold increase in total phenolic content and fourfold increase in antioxidant activity. The GSE addition resulted in no effect on the viability of Streptococcus thermophilus and Lactobacillus bulgaricus subsp. delbrueckii counts. The product with NE‐GSE had poor sensory properties and lesser polyphenol stability during storage as compared to En‐GSE yoghurt.     

Effect of flour type on Maillard reaction and acrylamide formation during toasting of bread crisp model systems and mitigation strategies

Maillard reaction along with caramelisation are the main chemical reactions occurring in bakery products. They are referred to as non enzymatic browning. In this work, the effect of flour type (wheat, rye and whole-wheat flours) and process conditions were investigated by using a bread crisp model system made up of flour, water and yeast. The bread was toasted at different temperature for different times. In a second set of experiments several additives (glycine, 0.1% on flour; asparaginase, 2000 U kg⁻¹ of flour and an antioxidant extract from green tea) were added to the basic formulations in order to test their ability in reducing the formation of potentially harmful Maillard reaction compounds such as HMF and acrylamide. Browning development and water content along with antioxidant activity were also monitored.Rye model systems produced more HMF and acrylamide at all temperature tested, while wholewheat systems produced less HMF, but more acrylamide than wheat one. The addition of glycine was effective in reducing acrylamide formation, and it increased browning development, antioxidant activity and HMF formation. Asparaginase reduced acrylamide formation up to 88% and had no effect on browning development and antioxidant activity. The addition of exogenous antioxidant compounds from green tea did not produce clear effect on acrylamide formation, thus it was not useful as mitigation strategy in bakery products, likely because of the low fat content.     

Quality of wheat bread enriched with onion extract and polyphenols content and antioxidant activity changes during bread storage

We determined the effect of onion skin extract (OE) on the quality of wheat bread: contents of total polyphenols and flavonols, antioxidative activity and their changes during storage. With increasing doses of OE to bread, contents of total polyphenols and flavonols and the antioxidative activity were observed to increase. A higher total flavonol content was found in bread with 1% OE stored at 24 °C than in bread stored at 4 °C. There was no such relationship in the bread with 0.5% OE. Changes in flavonols content during storage for 72 h were insignificant. No clear trends of change in the antioxidant activity could be found at storage of breads. The addition of OE caused no changes in the yield and volume of bread and contributed to a decreasing value of L* parameter and to increasing values of ΔE, a* and b* parameters of bread crumb colour. Consumers accepted the smell and taste of bread with the addition of OE to a lesser extent than the control bread. Bread with 1% OE the taste was rated very low. Consumers rated the taste of bread with 1% OE low because it was bitter. Breads enriched with OE had a proper external appearance as well as crumb and crust features. Roasted onion aroma and taste were perceptible in the obtained breads. A bitterness was perceptible in breads baked with the addition of 1% of OE.     

Effect of amino acids on Maillard reaction product formation and total antioxidant capacity in white pan bread

Maillard reaction products (MRPs) can be an important dietary antioxidant source. Bread is the most popular bakery product; however, limited information is available on the antioxidant activities of MRPs generated during bread-baking. This paper reports the effect of different amino acids on bread properties, melanoidin formation and antioxidant activities. Totally six amino acids at four different levels were evaluated. Increasing the amount of amino acid led to darker bread crust, higher melanoidin content and stronger antioxidant activity. Among the six amino acids, bread with glycine had the highest melanoidin content and ABTS radical scavenging activity up to 1079.77 ± 8.43 μmol TE mg⁻¹. Bread with lysine had the highest DPPH radical scavenging activity up to 281.97 ± 10.52 μmol TE mg⁻¹. Bread with alanine had the highest crust metal chelating activity compared with others. Adding certain dietary amino acids is a potential approach to enhance the antioxidant capacity of bread products.     

Sourdough on quinoa and buckwheat gluten-free breads: Evaluation of autochthonous starter fermentation on bread nutritional and technological properties

The aim of this work was to evaluate the effect of two autochthonous strains of lactic acid bacteria as sourdough (SD) starters on gluten-free bread technological quality and in vitro starch hydrolysis and antioxidant activity. Two strains of Limosilactobacillus fermentum isolated from buckwheat (BW) and quinoa flours were used. SDs were prepared from wholegrain quinoa or BW flours. Bread technological properties, total polyphenol content and in vitro antioxidant activity and starch hydrolysis and dialysability were assessed. Specific bread volumes were between 2.61 and 2.76 cm³ g⁻¹. Breads with quinoa-based SD had overall higher technological quality than breads made with BW SDs, shown by a softer, well-aerated crumb, with an opener crumb structure and larger air area. Crumb firming during storage was reduced up to 29% when quinoa SD was used, and up to 42% when BW SD was added. Quinoa breads showed higher polyphenols and FRAP values, whereas BW breads had significantly higher ABTS values. Total starch hydrolysis and dialysability were significantly reduced when SD was used. Starch hydrolysis was reduced up to 42% and 25% when quinoa and BW SD were used, respectively. SD application produced breads with higher technological quality and modified in vitro starch hydrolysis and antioxidant capacity. Even though these effects seemed to be influenced by pH, a biological effect was also observed.     

Physicochemical and sensory properties of soy bread made with germinated,steamed, and roasted soy flour

For the development of healthful gluten-free soy bread acceptable to consumers, we evaluated the effects of various processing procedures for soy flour on bread quality, in terms of beany flavour and texture. We pretreated soy flour by both non-heating (raw:NS and germinated:GS) and heating (steamed:SS and roasted:RS) methods. In addition, to improve the loaf volume, we added 1% hydroxypropyl-methylcellulose (HPMC) to RS flour. Lipoxygenase activity was retained in the non-heat-treated flours (279 U/g for NS and 255 U/g for GS), but was significantly reduced in the heat-treated flours (106 U/g for SS and 69 U/g for RS). Moreover, heat-treated flour had higher isoflavone and ferric reducing antioxidant power than had non-heat-treated flour. However, RS flour had the lowest moisture content and lowest L^∗ value. The GS bread had the highest specific loaf volume (3.53 cm³/g), followed by NS (2.96 cm³/g), RS (2.25 cm³/g), and SS (1.81 cm³/g) bread. GS bread had the lowest hardness (1.53 N), followed by NS (1.65 N), RS (2.00 N), and SS (3.75 N) bread. The addition of 1% HPMC to RS increased the loaf volume (2.44 cm³/g), but decreased the bread’s hardness (1.80 N). As to the sensory properties, the bread with heat-treated flour was perceived to have a less beany odour and taste than was the bread with non-heat-treated flour. However, the latter had a better appearance than the former. These results indicated that soy flour pretreatment could enhance the loaf volume and reduce the beany flavour of whole soy bread.     

番石榴叶提取物对无糖面包品质及抗氧化能力的影响

目的:开发一种无糖、抗氧化、低成本的面包。方法:将番石榴叶提取物和新橙皮苷二氢查耳酮作为功能性配料添加到面包中,通过检测面包的比容、质构、保水性能、自由基清除能力对其品质和抗氧化能力进行分析。结果:番石榴提取物的添加量与面包综合品质之间不存在明显的线性关系,但是番石榴提取物的添加量与面包的总酚含量、DPPH自由基和羟基自由基清除率呈正相关。当番石榴叶提取物添加量为7.5%时,制备出的面包综合品质最佳、感官评价达78.6分,且面包总酚含量、DPPH自由基和羟基自由基清除率能力分别提升了85%,70.3%,141.7%,面包具有较强抗氧化能力。结论:添加番石榴叶提取物能够改善无糖面包品质,大幅提升其抗氧化能力。     

Effect of combination of essential oils on physicochemical and sensorial attributes of burfi in comparison with individual essential oil and BHA

The study investigated the addition of essential oils (EO) of tumeric, ginger and cardamon (as sources of natural antioxidants ) to burfie, an Indian heat desiccated dairy dessert. Essential oil incorporation significantly (P < 0.05) increased antioxidant activity of the product. Samples with a combination of EOs had a higher antioxidant activity than with addition of individual EOs, but antioxidant activity was less (P < 0.05) than samples with addition of butylated hydroxyanisole (BHA). The addition of EOs had no significant (P > 0.05) effect on instrumental colour, textural attributes and composition of the burfi samples, but flavour was significantly affected. Clustering revealed the sample with added BHA had the best attributes followed by the sample with addition of a combination of all the EOs. The combination of EOs significantly (P < 0.05) delayed the growth of the native microbiota in the burfi.     

Antibacterial Effect of Grapefruit Seed Extract (GSE) on Makgeolli‐Brewing Microorganisms and Its Application in the Preservation of Fresh Makgeolli

To develop a new preservation method, the antimicrobial activity of grapefruit seed extract (GSE) against Makgeolli‐brewing microorganisms and food‐borne pathogens was assessed, and a general analysis and sensory evaluation of fresh Makgeolli with added GSE was made. The minimum inhibitory concentration (MIC) values of GSE against 10 strains of Makgeolli‐brewing microorganism were 0.0122 to 1.5625 μL/mL. The MIC values against 6 strains of food‐borne pathogens were 0.0061 to 0.7813 μL/mL. On addition of 0.1% (v/v) and 0.2% GSE in bottled fresh Makgeolli, no significant difference in the pH, or the contents of total acids, ethanol, or methanol in the Makgeolli, were observed compared with control Makgeolli (with no GSE), during the preservation period (8 weeks) at 10 °C. In the Makgeolli with 0.1% and 0.2% GSE, the total bacterial counts decreased significantly by 4.9% (P < 0.01) and 11.2% (P < 0.001), respectively, versus the control. The decreases in yeast count were significantly lessened by 15.33% and 15.24% (both P < 0.001), respectively, after 8 weeks of storage, compared with the control. In the sensory evaluation of Makgeolli with 0.1% and 0.2% GSE, the refreshment and overall acceptability received significantly better scores than the control (P < 0.01), with no change in sweetness, bitterness, sourness, turbidity, color, or odor. These results suggest that GSE controls the growth of Makgeolli‐brewing microorganisms and extends the shelf life (ca. 2 wk), without decreasing overall acceptance.     

Optimisation of sourdough bread incorporation into wheat bread by response surface methodology: Bioactive and nutritional properties

The optimisation of the quantity of sourdough (A) prepared with two different fermentation methods, [spontaneous fermentation (F1) vs. starter of lactic acid bacteria‐added fermentation (F2)], instant active dry yeast (B) and wheat bran (C) for a nutritionally improved bread formulation has been studied by evaluating the bioavailability and bioactive properties. The bread produced according to the optimised formula and fermentation types of F1 (OB_F1) and F2 (OB_F2) was compared with control bread (CB). The optimised levels for F1 were 11.45% for sourdough, 1.10% for dry yeast and 1.58% for wheat bran and for F2 6.99% for sourdough, 1.02% for dry yeast and 38.84% for wheat bran. The addition of sourdough significantly (P < 0.05) affected antioxidant activity, total phenolic content, in vitro ash and protein digestibility, and enzyme resistance starch contents of bread. The F1 fermentation method was found to be more effective in terms of bread properties examined.     

Characterization of antioxidant chitosan film incorporated with Zataria multiflora Boiss essential oil and grape seed extract

Antioxidant chitosan based edible films were developed incorporated with Zataria multiflora Boiss essential oil (ZEO) (5 and 10 g/L) and grape seed extract (GSE) (10 g/L) alone and in combination. The physico-mechanical, wettability, swelling index, color, total phenol and antioxidant characteristics of the films were investigated. Films without any agents were used as control sample. All films, with the exception of 10 g/L GSE + 10 g/L ZEO film, exhibited lower strength and elongation values, and only the addition of 10 g/L ZEO to GSE film improved the water vapor transmission rate of chitosan films. Only GSE containing films had higher swelling index. The incorporation of GSE and ZEO into chitosan film increased the wettability of the surface, total phenol and antioxidant activity. Neat chitosan and ZEO incorporated films had a light yellowish color, whereas GSE + ZEO films were gray.     

Effect of phenolic acids on the rheological properties and proteins of hard wheat flour dough and bread

BACKGROUND: The effects of different phenolic acids on the rheological properties and gluten proteins of hard wheat flour dough and bread were investigated. Caffeic, ferulic, syringic and gallic acids were each blended with hard wheat flour at a concentration of 4.44 µmol L^?1 g^?1 flour. RESULTS: Mixing time and tolerance were reduced with the addition of phenolic acids. The phenolic acids reduced the maximum resistance to extension (R_max) and increased the extensibility of dough, with effects in the following order: gallic < syringic < ferulic < caffeic acid. The effect on R_max was more pronounced in overmixed dough. Loaf volume was most significantly decreased with the addition of caffeic acid. Extraction of sodium dodecyl sulfate‐soluble high‐molecular‐weight proteins was increased in both mixed and fermented doughs by the addition of ferulic and caffeic acids. The order of influence of the phenolic acids on the rheological properties and protein structure of dough and bread was consistent with that of their antioxidant activity. CONCLUSION: The addition of caffeic and ferulic acids reduced R_max and increased the extensibility of hard wheat flour dough by modifying the high‐molecular‐weight gluten, which resulted in decreased bread volume. Copyright © 2011 Society of Chemical Industry     

Effectiveness of Zataria multiflora Boiss essential oil and grape seed extract impregnated chitosan film on ready-to-eat mortadella-type sausages during refrigerated storage

BACKGROUND: The effectiveness of chitosan films containing Zataria multiflora Boiss essential oil (ZEO) (5 and 10 g kg^?1) and grape seed extract (GSE) (10 g kg^?1) on lipid oxidation and microbial (lactic acid bacteria, aerobic mesophiles and inoculated Listeria monocytogenes) characteristics of mortadella sausage at 4 °C for 21 days was evaluated. The release of total phenolics (TPs) into sausage was also assessed. RESULTS: All films exhibited antibacterial activity against L. monocytogenes on agar culture media. Chitosan films containing ZEO were the most effective on the growth of bacteria. The growth of L. monocytogenes was significantly inhibited by ZEO‐GSE containing films especially during storage of the sausages for 6 days. Aerobic mesophiles and lactic acid bacteria were the most sensitive and resistant groups to films by 0.1–1.1 and 0.1–0.7 log cycles reduction, respectively. Sausages wrapped by 10 g kg^?1 GSE + 10 g kg^?1 ZEO films had the lowest degrees of lipid oxidation, which was 23% lower than the control. The TPs of ZEO films decreased to zero after 6 days, whereas TPs of GSE films followed a slight decrease during the storage. CONCLUSION: Antimicrobial/antioxidant chitosan film could be developed by incorporating GSE and ZEO for extending the shelf life of mortadella sausage. Copyright © 2011 Society of Chemical Industry     

Impact of thermostable amylases during bread making on wheat bread crumb structure and texture

Thermostable amylases [Bacillus subtilis α-amylase (BSuA) and B. stearothermophilus maltogenic amylase (BStA)] with different modes of action and impacts on firming properties were added during straight-dough bread making. BSuA continuously degraded the starch fraction during bread making. Its action resulted in larger gas cells than in control bread, but did not change initial firmness. In contrast to BSuA, BStA mainly degraded starch at the end of the baking phase and during bread cooling, which caused little if any impact on bread crumb texture. However, it led to higher initial firmness readings than for the control breads. Neither BSuA nor BStA were inactivated during bread making. The results evidence that starch properties have a large impact on bread crumb structure and initial firmness and are highly influenced by the mode of action of the enzyme.     

Bread and durum wheat compared for antioxidants contents,and lipoxygenase and peroxidase activities

Potential beneficial components, including proteins, total phenolics, total flavonoids, carotenoids, tocopherols, and DPPH radical scavenging activity, were investigated in wholemeal of ten bread (T. aestivum L.) and ten durum (T. durum Desf.) novel wheat genotypes. In addition, the activity rate of lipoxygenase (LOX) and peroxidase (POD) enzymes implicated in the antioxidant metabolism was determined. The protein contnet and the antioxidant properties varied according to the two different wheat species, as well as, between the different bread and durum wheat genotypes themselves. The results indicated significant differences in proteins and antioxidant compounds between bread and durum wheat. Higher total proteins, wet gluten and antioxidants contents, combined with lower LOX and POD activities, point to a higher nutritive value of durum wheat than bread wheat.     

Flavour of sourdough wheat bread crumb

This study investigates the effect of adding sourdough to wheat bread dough on the production of flavour compounds in wheat bread crumb. The sourdoughs were fermented with starter cultures of lactic acid bacteria alone and in combination with sourdough yeasts. The volatile compounds in the bread crumb were isolated by a dynamic headspace technique and extraction analysis, analysed by gas chromatography (GC), and identified on the basis of GC retention times for reference compounds and mass spectrometry (MS). The chemical analyses were combined with sensory evaluation. The volume of the loaves increased significantly when the doughs had 5–20% sourdough added compared with the control bread (bread without sourdough). In the sourdough bread, the content of acetic acid, 2-methylpropanoic acid, and 3-methylbutanoic acid was generally higher, and loaves made with the addition of sourdoughs fermented withLactobacillus plantarum, L. delbrueckii, orL. sanfrancisco had a higher content of 2- or 3-methyl-1-butanol than control bread. Interactions were seen between the starter cultures and the sourdough yeasts, and the production of the following compounds was increased depending on the starter culture used and on the sourdough yeast: ethanol, 2-methylpropanol, 2/3-methyl-1-butanol, 2-phenylethanol, benzyl alcohol, acetic acid, 2-methylpropanoic acid, and 3-methylpropanoic acid. Bread made with an addition of 5% to 15% sourdough fermented withL. sanfrancisco had a pleasant, mild and sour odour and taste.L. plantarum bread had a strong, sour and unpleasant odour and a metallic sour taste with a sour aftertaste, but when the sourdough was also supplemented with the sourdough yeastSaccharomyces cerevisiae, the bread attained a more aromatic wheat bread flavour, which may be caused, in part, by a higher content of 2/3-methyl-1-butanol, 2-methylpropanoic acid, 3-methylbutanoic acid and 2-phenylethanol.     

Changes in polyphenolic content and antioxidant activity after thermal treatments of grape seed extract and grape pomace

This study was undertaken to investigate the effect of heat treatment on polyphenolic content and antioxidant activity in grape pomace (GP) and grape seed extract (GSE). GP and GSE were heat treated in a furnace or autoclaved at 100 °C for 15, 30 and 60 min. Structural modifications of the polyphenols during thermal processes were determined by HPLC–MS, and the antioxidant activity was determined by ABTS, DPPH and photochemiluminescence methods. In general, furnace thermal treatment of GSE and GP did not affect the total extractable polyphenols content, tannin content, procyanidin components and the antioxidant activity. Autoclave treatment caused an extensive hydrolysis of gallocatechin (70%), catechin (61%), epicatechin (65%), procyanidin B1 (75%) and procyanidin B2 (73%) in GSE, and an increase in gallic acid (71%), gallocatechin (100%) and epicatechin gallate (129%) in GP. Autoclave treatment did not affect the antioxidant activity of GSE or GP. It can be concluded that the effect of autoclave was more severe than furnace heat treatment modifying the phenolic profile in a different manner depending on the grape seed product used. These modifications were not related with changes in antioxidant activity.     

Antioxidant activity and functional properties of porcine plasma protein hydrolysate as influenced by the degree of hydrolysis

Antioxidant activity and functional properties of porcine blood plasma protein hydrolysates (PPH) prepared with Alcalase at 6.2%, 12.7% and 17.6% of degree of hydrolysis (DH) were investigated. The PPH showed stronger radical-scavenging ability and possessed stronger Cu²⁺-chelation ability and a reducing power compared to non-hydrolysed plasma protein (P < 0.05). The antioxidant activity of PPH, indicated by thiobarbituric acid-reactive substance (TBARS) values in a liposome-oxidising system, increased with increasing DH (P < 0.05). The Alcalase hydrolysis increased protein solubility from its original 68.46–81.79% (non-hydrolysed) to 82.95–94.94% (hydrolysed) over a broad pH range (3.0–8.0). However, hydrolysis decreased surface hydrophobicity and suppressed emulsifying and foaming capacity of the plasma protein. To identify antioxidant peptide, PPH was subjected to ultrafiltration, ion-exchange chromatography and reverse-phase high performance liquid chromatography (RP-HPLC), and the amino acid sequences of isolated peptides were determined by liquid chromatography/tendem mass spectrometry (LC–MS/MS). The peptide with the strongest antioxidant activity had the amino acid sequence of His-Asn-Gly-Asn. The results indicated that PPH could be used as a novel antioxidant but may be of limited utility as an emulsifying or foaming agent.