共查询到19条相似文献,搜索用时 93 毫秒
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目的:通过体外抗氧化测试,评价五味子藤总木脂素固形物的抗氧化活性。方法:采用分光光度法检测五味子藤总木脂素固形物的总抗氧化能力(T-AOC)及对超氧阴离子自由基的清除能力。结果:五味子藤总木脂素固形物的总抗氧化值及对超氧阴离子自由基的抑制率均呈现浓度依赖性;当浓度为0.2 mg/mL时,总抗氧化值为(8.89±0.34) U/mg,约为同浓度VC的1/5,对超氧阴离子自由基的抑制率为(26.932±1.031)%,约为同浓度VC的1/4。结论:五味子藤总木脂素固形物具有一定的抗氧化活性。 相似文献
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采用索氏提取法提取水浸预处理后的五味子中的木脂素,考察了虹吸次数、液固比、颗粒粒径及乙醇浓度对萃取率的影响,采用响应面法进行优化. 结果表明,五味子醇甲的最佳提取条件为:乙醇浓度98.05%(j),液固比67.20 mL/g,颗粒平均粒度0.33 mm,该条件下理论萃取率为80.90%,实际萃取率为77.17%;五味子甲素的最佳提取条件为:乙醇浓度96.38%(j),液固比72.52 mL/g,颗粒平均粒度0.21 mm,该条件下五味子甲素理论萃取率为77.11%,实际萃取率为74.31%;五味子乙素的提取最佳条件为:乙醇浓度96.38%(j),液固比72.52 mL/g,颗粒平均粒度0.21 mm,该条件下理论萃取率为77.13%,实际萃取率为74.23%. 各因素对木脂素提取率影响的显著性顺序为乙醇浓度>液固比>颗粒粒径. 相似文献
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以二氯乙烷作溶剂,炔酸烯丙酯在K2CO3催化下,可以顺利发生分子内串联环化反应得标题化合物,产率65% ~88%.产物经1HNMR、13CNMR和GC-MS确证. 相似文献
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天然木脂素是由含酚羟基的苯丙素类化合物在生物酶催化下,通过氧化偶联反应而形成的。因此,以氧化偶联反应为关键步骤来仿生合成各类木脂素是最常用也是最快捷的合成途径。文章将就如何利用氧化偶联反应,在生物体外进行简单快捷的构建芳基萘类木脂素的基本骨架结构展开相关论述。 相似文献
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一种从富含多酚多糖的西葫芦组织中快速提取RNA的方法 总被引:1,自引:0,他引:1
对比研究了一步法、尿素/氯化锂法、热酚法、LUG法等4种方法,建立了一种适合西葫芦组织RNA提取的改进的CTAB方法。用该方法从西葫芦叶中提取的RNA在琼脂糖凝胶电泳上清楚显示出28S和18S两条链.A250/280值为2.08、A250/280值为2.0,表明提取的RNA没有多酚、多糖、蛋白质污染;RNA产量为210μg·g^-1鲜质量;对RT—PCR的结果进行测序,通过NCBI—BLAST比对可知,与印度南瓜的同源性达89%。用该方法从西葫芦果肉中也获取了高质量的RNA。改进的CTAB法全程只需2h,不用液氮、亚精胺、二硫苏糖醇、蛋白酶K等试剂。 相似文献
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Gilbert O. Fruhwirth Albin Hermetter 《European Journal of Lipid Science and Technology》2007,109(11):1128-1140
Cucurbita pepo subsp. pepo var. Styriaca is a phylogenetically young member of the Cucurbita spp. since the mutation leading to dark green seeds with stunted outer hulls arose only in the 19th century. This mutation defined the so‐called Styrian oil pumpkin and facilitated the production of Styrian pumpkin seed oil. It is a regional specialty oil in the south‐eastern part of Europe. In this article, we describe the production and economic value of this edible oil as well as its composition on a molecular basis, including fatty acids, vitamins, phytosterols, minerals, polyphenols, and the compounds responsible for its pigments, taste and flavor. We also describe contaminants of Styrian pumpkin seed oil and the most relevant field pests of the Styrian oil pumpkin plant. Finally, we review the putative beneficial health effects of Styrian oil pumpkin seeds and of their products. 相似文献
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Biologically Active Digests from Pumpkin Oil Cake Protein: Effect of Cross-linking by Transglutaminase 下载免费PDF全文
Ljiljana Popović Žužana Stolić Jelena Čakarević Aleksandra Torbica Jelena Tomić Mirjana Šijački 《Journal of the American Oil Chemists' Society》2017,94(10):1245-1251
The objective of this study was to show that biologically active hydrolysates can be obtained by simulated human gastrointestinal digestion (HGD) of transglutaminase cross-linked pumpkin oil cake protein (Tg-C) which was previously reported as a potential functional food additive. A two-stage in vitro digestion model system (by pepsin and α chymotrypsin and trypsin, simultaneously) was used to simulate the process of HGD on native and Tg-C major storage pumpkin oil seed/cake protein, cucurbitin (C). The biologically active potential of the digests was evaluated, measuring the angiotensin-converting-I enzyme (ACE) inhibitory and anti-oxidant capacity. The ACE inhibitory activity was determined in both final digests, with IC50 = 0.30 ± 0.04 mg/ml for C and IC50 = 0.28 ± 0.01 for Tg-C. The anti-oxidant potency of the examined proteins was enhanced by the digestion process. The 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation activities and reducing power testing showed that all the hydrolysates act as a radical quencher and reducing agents. Overall, the results showed that the cross-linking by Tg did not influence the digestion process, as well as having no effect on the biological activity of the hydrolysates. These also indicate that Tg-C, if used as functional food additive, after food consumption can be digested and become a source of peptides exerting positive effects on human health. 相似文献
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Chaojie Wang Wenling Li Fangyuan Chen Yaqian Cheng Xin Huang Bingxue Zou Yunli Wang Wenlong Xu Shuping Qu 《International journal of molecular sciences》2022,23(15)
Ethylene biosynthesis and signal transduction play critical roles in plant sex differentiation. ACS (1-aminocyclopropane-1-carboxylic acid synthase) is a rate-limiting enzyme in ethylene biosynthesis. However, the understanding of the ACS gene family in Cucurbita maxima is limited. Here, we identified and characterized 13 ACS genes in the C. maxima genome. All ACS genes could be divided into three groups according to a conserved serine residue at the C-terminus. Thirteen CmaACS genes were found to be randomly distributed on 10 of the 20 chromosomes of C. maxima. The ACS gene exhibits different tissue-specific expression patterns in pumpkin, and four ACS genes (CmaACS1, CmaACS4, CmaACS7, and CmaACS9) were expressed specifically in both the female and male flowers of C. maxima. In addition, the expression levels of CmaACS4 and CmaACS7 were upregulated after ethephon and IAA treatments, which ultimately increased the number of female flowers, decreased the position of the first female flower and decreased the number of bisexual flowers per plant. These results provide relevant information for determining the function of the ACS genes in C. maxima, especially for regulating the function of ethylene in sex determination. 相似文献
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Fathi T. Halaweish 《Journal of chemical ecology》1993,19(1):29-37
In addition to cucurbitacins E andI, cucurbitacins D,1, 3-epi-isocucurbitacin D,2, and B,3, were isolated from the fruits ofCucurbita texana and structurally identified by UV, IR,1H NMR,13C NMR, and MS, and 2-O--glucopyranosylcucurbitacin I was identified. These compounds have not been reported previously as constituents of this species. The isolation of 3-epi-isocucurbitacin D2 together with normal cucurbitacins suggests that isocucurbitacins occur naturally. Evidence is also discussed that isocucurbitacins are biosynthesized one step ahead of normal cucurbitacin. 相似文献
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超临界流体CO2萃取南瓜籽油的初步研究 总被引:4,自引:0,他引:4
以超临界流体CO2萃取技术对南瓜籽中的油脂提取进行了初步研究.研究了不同物料粒度、萃取压力、萃取温度、萃取时间及CO2流量对南瓜籽油萃取率的影响. 相似文献